Study on the Regulation of ROS by Acupuncture and Moxibustion to Relieve Inflammation in the Treatment of Sciatica

Objective: To observe the analgesic effect of acupuncture and moxibustion on chronic constriction injury (CCI) pain model rats, and to investigate the mechanism of constriction constriction injury. Methods: 32 SPF SD rats were divided into Control Group, Sham Surgery Group, Model Group and Acupuncture and moxibustion Group, with 8 rats in each group. The Model Group, Acupuncture and moxibustion Group and sciatic nerve ligation were used to establish the CCI pain rat model. The sham operation group only separated the nerve, and the control group did not do any treatment. After modeling, the Acupuncture and moxibustion Group was given acupuncture treatment, while the control group and the Model Group did not do any intervention. 7 days for 1 course of treatment, continuous treatment for 3 courses of sampling and detection. Paw withdrawal mechanical threshold (PWMT) and Paw withdrawal thermal latency (PWTL) were observed. Biochemical detection of reactive oxygen species (ROS), malondialdehyde (MDA), Super Oxide Dismutase (SOD), glutathione (GSH);Serum, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) levels were detected by ELISA. Results: Compared with the control group, PWMT and PWTL in the Model Group were decreased (P α, IL-1β and IL-6 levels or contents were increased (P α, IL-1β and IL-6 levels or contents were decreased (P Conclusion: Acupuncture and moxibustion can reduce the ROS level, enhance the antioxidant capacity of the body, reduce the damage of sciatic nerve caused by inflammation, and relieve sciatic pain.

Identification of subsurface damage of 4H-SiC wafers by combining photo-chemical etching and molten-alkali etching

In this work,we propose to reveal the subsurface damage(SSD)of 4H-SiC wafers by photo-chemical etching and identify the nature of SSD by molten-alkali etching.Under UV illumination,SSD acts as a photoluminescence-black defect.The selective photo-chemical etching reveals SSD as the ridge-like defect.It is found that the ridge-like SSD is still crystalline 4H-SiC with lattice distortion.The molten-KOH etching of the 4H-SiC wafer with ridge-like SSD transforms the ridge-like SSD into groove lines,which are typical features of scratches.This means that the underlying scratches under mechanical stress give rise to the formation of SSD in 4H-SiC wafers.SSD is incorporated into 4H-SiC wafers during the lapping,rather than the chemical mechanical polishing(CMP).

Ultrahigh energy storage with superfast charge-discharge capability achieved in linear dielectric ceramic

Ceramic capacitors designed for energy storage demand both high energy density and efficiency.Achiev-ing a high breakdown strength based on linear dielectrics is of utmost importance.In this study,we present the remarkable performance of densely sintered(1-x)(Ca_(0.5)Sr_(0.5)TiO_(3))-xBa4Sm28/3Ti18O54 ceramics as energy storage materials,with a measured energy density(Wrec)of 4.9 J/cm^(3)and an ultra-high ef-ficiency(η)of 95%which is almost optimal in linear dielectric that has been reported.To unravel the underlying mechanisms,we conducted a systematic investigation on the influence of adding paraelec-tric Ba_(4)Sm_(28/3)Ti_(18)O_(54)(BST)on both microstructure and macroscopic electrical properties of Ca_(0.5)Sr_(0.5)TiO_(3)(CST).Notably,the addition of BST effectively reduces the grain size of CST.The conduction mechanism is primarily governed by grain boundaries,where high-density grain boundaries act as barriers to charge carrier transport due to their elevated resistivity.Moreover,the activation energy associated with grain boundaries increases with rising resistivity,implying a lower concentration of free vacancies within these regions.The increased barrier height for oxygen vacancy migration at grain boundaries compensates for the grain boundary defects,thereby resulting in enhanced breakdown strength.This characteristic offers a substantial advantage in terms of thermal and frequency stability(25-175℃,1-100 Hz).This work introduces a candidate material with outstanding comprehensive energy storage properties.

Zhuangtongyin Ameliorates Cardiomyocyte Pyroptosis in Rats with Coronary Heart Disease by Regulating Caspase-1

Background: Recent studies have demonstrated that pyroptosis has an important impact on the pathological process of many cardiovascular diseases, and inhibiting this process can significantly reduce pathological damage. At the same time, some studies have proved that Zhuangtongy in (ZTY) has a certain protective effect on the myocardium of rats with coronary heart disease. Therefore, the aim of this study is to observe and explore the effects of ZTY on cardiomyocytes and its mechanism of action in rats with coronary heart disease based on the Caspase-1 mediated cellular pyroptosis pathway. Methods: Fifty SD rats were randomly divided into blank group, sham-operation group, model group, ZTY group and cysteinyl aspartate specific protease (Caspase-1) inhibitor group. The blank group did not receive any treatment, and the other groups established a rat model of coronary artery disease by ligating the left anterior descending branch of the coronary artery, but the sham-operation group was only threaded without ligation. After that, the sham-operated and model groups were gavaged with 0.1 ml/kg distilled water, the ZTY group was gavaged with 13.6 g/kg ZTY decoction based on the previous study, and the Caspase-1 inhibitor group was injected intraperitoneally with 3 mg/kg Ac-YVAD-cmk solvent once a day for four consecutive weeks. Transmission electron microscopy was used to observe the ultrastructure of cardiomyocytes, HE staining was used to observe the morphology of cardiac tissue, and enzyme-linked immunosorbent assay (Elisa) was used to detect serum interleukin-1β (IL-1β), interleukin-18 (IL-18) and C-reactive protein (CRP) in the rats. In addition, fluorescent probe was used to detect serum Reactive oxygen species (ROS). Real-time fluorescence quantitative PCR was used to detect the expression of mRNAs of ASC, Nlrp3, Caspase-1 and gasdermin-D (GSDMD), and the protein expression of Caspase-1 and GSDMD was detected by immunohistochemistry. Results: There was no significant difference between the results of the blank and sham-operated groups. Compared with the blank group, the transmission electron microscopy results showed swollen and ruptured cardiomyocyte membranes in the model group, with pore formation, severe mitochondrial swelling, membrane lysis, and cristae breakage. The staining results showed myofibril breakage, severe intercellular oedema and vacuolation in the model group. The inflammatory factors IL-1β, IL-18, CRP and ROS were significantly elevated (P β, IL-18, CRP, ROS and the expression of ASC, Nlrp3, Caspase-1, and GSDMD mRNA were significantly elevated (P Conclusion: ZTY can reduce myocardial injury by lowering the expression of ROS and other inflammatory factors, inhibiting Caspase-1 mediated cellular pyroptosis, thus reducing myocardial inflammation and protecting cardiomyocytes.

THE NONCONFORMING CROUZEIX-RAVIART ELEMENT APPROXIMATION AND TWO-GRID DISCRETIZATIONS FOR THE ELASTICEIGENVALUE PROBLEM

In this paper,we extend the work of Brenner and Sung[Math.Comp.59,321–338(1992)]and present a regularity estimate for the elastic equations in concave domains.Based on the regularity estimate we prove that the constants in the error estimates of the nonconforming Crouzeix-Raviart element approximations for the elastic equations/eigenvalue problem are independent of Laméconstant,which means the nonconforming Crouzeix-Raviart element approximations are locking-free.We also establish two kinds of two-grid discretization schemes for the elastic eigenvalue problem,and analyze that when the mesh sizes of coarse grid and fine grid satisfy some relationship,the resulting solutions can achieve the optimal accuracy.Numerical examples are provided to show the efficiency of two-grid schemes for the elastic eigenvalue problem.