期刊文献+
共找到2篇文章
< 1 >
每页显示 20 50 100
造血干细胞Hdac3基因促进鼠淋巴细胞分化
1
作者 吕文琼 舒逸 +7 位作者 杨碧洁 张航 张佳 张虹洋 周永杰 石毓君 邹琳 张鹏辉 《第三军医大学学报》 CAS CSCD 北大核心 2019年第9期844-850,共7页
目的建立造血干细胞特异性Hdac3条件性敲除小鼠模型,探讨Hdac3基因对小鼠造血发育的影响。方法 Hdac3^(fl/fl)小鼠与Mx1-Cre小鼠杂交获得Hdac3^(fl/fl)Mx1-Cre小鼠,PCR鉴定小鼠基因型。连续注射poly(I:C),诱导Cre表达,得到造血干细胞Hd... 目的建立造血干细胞特异性Hdac3条件性敲除小鼠模型,探讨Hdac3基因对小鼠造血发育的影响。方法 Hdac3^(fl/fl)小鼠与Mx1-Cre小鼠杂交获得Hdac3^(fl/fl)Mx1-Cre小鼠,PCR鉴定小鼠基因型。连续注射poly(I:C),诱导Cre表达,得到造血干细胞Hdac3敲除鼠Hdac3^(fl/fl)Mx1-Cre^+(CKO)及对照组小鼠Hdac3^(+/+)Mx1-Cre^+(WT),Q-PCR分析Hdac3 mRNA表达水平。流式细胞术检测小鼠外周血淋巴细胞数量及比例。HE染色观察小鼠胸腺、脾脏形态学改变。流式细胞术检测小鼠外周血CD3^+、B220^+细胞比例。克隆形成实验分析小鼠造血干/祖(LSK)细胞的分化能力。结果成功建立造血干细胞Hdac3^(fl/fl)Mx1-Cre^+(CKO)小鼠模型。与对照组相比,CKO小鼠外周血淋巴细胞绝对数量(P<0.001)及比例(P<0.01)均显著降低;细胞亚群分析显示,CKO小鼠外周血CD3^+淋巴细胞及B220^+淋巴细胞显著减少(P<0.01,P<0.001),CD11b^+髓系细胞比例显著增加(P<0.01)。HE染色结果显示,实验组鼠胸腺和脾淋巴细胞数减少。克隆形成实验显示,实验组小鼠粒系克隆数无差异,前B细胞样克隆缺失。结论造血干细胞中Hdac3基因促进造血干细胞向淋巴细胞分化。 展开更多
关键词 组蛋白去乙酰化酶3 造血干细胞 分化 淋巴细胞发育
下载PDF
Influence of Real Gas Properties on Aerodynamic Losses in a Supercritical CO_(2) Centrifugal Compressor
2
作者 yang Mingyang CAI Ruikai +2 位作者 ZHUGE Weilin yang bijie ZHANG yangjun 《Journal of Thermal Science》 SCIE EI CAS CSCD 2024年第6期2032-2046,共15页
Supercritical carbon dioxide(SCO_(2))centrifugal compressor is a key component of a closed Brayton cycle system based on SCO_(2).A comprehensive understanding of the loss mechanism within the compressor is vital for i... Supercritical carbon dioxide(SCO_(2))centrifugal compressor is a key component of a closed Brayton cycle system based on SCO_(2).A comprehensive understanding of the loss mechanism within the compressor is vital for its optimized design.However,the physical properties of SCO_(2) are highly nonlinear near the critical point,and the internal flow of the compressor is closely related to its properties,which inevitably influences the generation of aerodynamic losses within the compressor.This paper presents a comprehensive investigation of the compressor's loss mechanism with an experimentally validated numerical method.The real gas model of CO_(2) embodied in the Reynolds-Averaged Navier-Stokes(RANS)model was used for the study.Firstly,the numerical simulation method was validated against the experimental results of Sandia SCO_(2) compressor.Secondly,performance and loss distribution of the compressor were compared among three fluids including SCO_(2),ideal CO_(2)(ICO_(2))and ideal air(IAir).The results showed that the performance of SCO_(2) was comparable to IAir under low flow coefficient,however markedly inferior to the other two fluids at near choke condition.Loss distribution among the three fluids was distinctive.In the impeller,SCO_(2) was the most inefficient,followed by ICO_(2) and IAir.The discrepancies were magnified as the flow coefficient increased.This is due to a stronger Blade-to-Blade pressure gradient that intensifies boundary layer accumulation on walls of the shroud/hub.Furthermore,owing to the reduced sonic speed of SCO_(2),a shock wave appears earlier at the throat region and SCO_(2) encounters more intenseboundarylayerseparation. 展开更多
关键词 SCO_(2) centrifugal compressor loss mechanism real gas properties
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部