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Effect of Long-Term Fertilization on Soil Productivity and Nitrate Accumulation in Gansu Oasis 被引量:15
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作者 yang sheng-mao LI Feng-min +4 位作者 SUO Dong-rang GUO Tian-wen WANG Jian-guo SONG Bing-ling JIN Shao-ling 《Agricultural Sciences in China》 CAS CSCD 2006年第1期57-67,共11页
A long-term (1982-2001) field experiment was conducted in a calcareous soil under wheat (Triticum aestivum L.)-wheat (Triticum aestivum L.)-maize (Zea mays L.) rotation system at Zhangye, Gansu Province, China... A long-term (1982-2001) field experiment was conducted in a calcareous soil under wheat (Triticum aestivum L.)-wheat (Triticum aestivum L.)-maize (Zea mays L.) rotation system at Zhangye, Gansu Province, China to determine the effects of long-term fertilization on crop yield, nutrients interactions, content and accumulation of nitrate-N in soil profiles. Twenty- four plots in a split-plot factorial with a combination of eight treatments (from nitrogen (N), phosphorus (P), potassium (K) and farmyard manure (M) applications) and 3 replications were selected. Main treatments were M and without M, and the sub-treatments were no-fertilizer (CK), N, NP and NPK. When P and K fertilizers were part of treatments, their ratio to N was 1N:0.22P:0.42K. All M, P and K fertilizers were applied as the basal dressing. The grain yield was harvested each experimental period and straw yield for the period from 1988 to 1997. After crop harvest in 2000, the soil was sampled from the 0-20, 20-60, 60-100, 100-140 and 140-180 cm depths to determine NO3^--N content. Maize yield of CK in 2000 was only 28.2% of that in 1984, and wheat in 2001 was 25.7% of that observed in 1982. Average impact of fertilizers on grain yield decreased in the order of N 〉 M 〉 P 〉 K. Yield response to N and P fertilizers increased with progress of the experiment. The impact of K fertilizer showed no increase in grain yield during the initial 6 years (1982-1987), moderate increase in the next 5 years (1988-1992), and considerable increase in the last 9 years (1993-2001). The straw yield trend was similar to grain yield. Accumulation and distribution of NO3^--N in soil was significantly affected by annual fertilizations. Mineral fertilizers (NP and NPK) led to NO3^- -N accumulation in most subsoil layers, with major impact in the 20-140 cm depth. The combination of mineral fertilizers and farmyard manure (MNP and MNPK) reduced soil NO3^--N accumulation in comparison to mineral fertilizers, It can be argued that long-term fertilization significantly enhanced grain and straw yield in this rotation scheme. The findings of this research suggest that it is important to balance application of mineral fertilizers and farmyard manure in order to protect soil and underground water from potential NO3^--N pollution while sustaining high productivity in the oasis agro-ecosystem. 展开更多
关键词 long-term fertilization accumulation of nitrate-N crop yields nutrients interactions farmyard manure Nbalance sheet Zhangye Oasis
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长期生物炭添加对稻田土壤细菌和真菌反硝化N_(2)O排放的影响 被引量:1
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作者 王梦洁 蒋文婷 +6 位作者 徐有祥 刘玉学 吕豪豪 汪玉瑛 杨生茂 何莉莉 蔡延江 《环境科学》 EI CAS CSCD 北大核心 2024年第8期4923-4931,共9页
由细菌和真菌驱动的反硝化作用是稻田土壤氧化亚氮(N_(2)O)排放的主要来源,一般认为生物炭通过影响细菌反硝化过程来减少N_(2)O排放,而对真菌反硝化的相关影响机制尚不清楚.以中国科学院常熟农业生态实验基地长期秸秆炭化还田试验田为对... 由细菌和真菌驱动的反硝化作用是稻田土壤氧化亚氮(N_(2)O)排放的主要来源,一般认为生物炭通过影响细菌反硝化过程来减少N_(2)O排放,而对真菌反硝化的相关影响机制尚不清楚.以中国科学院常熟农业生态实验基地长期秸秆炭化还田试验田为对象,通过室内厌氧培养和分子生物学技术研究了长期不同生物炭施加量下(空白、2.25 t·hm^(-2)和22.5 t·hm^(-2),分别用BC0、BC1和BC10表示)稻田土壤细菌和真菌反硝化产生N_(2)O的相对贡献及相关微生物的作用机制.结果表明,与BC0相比,生物炭处理显著降低了N_(2)O排放速率、反硝化势以及N_(2)O累积排放量,且3个处理中细菌反硝化贡献均大于真菌反硝化;其中,BC10的细菌反硝化相对贡献率(62.9%)相较于BC0(50.8%)显著增加,BC10的真菌反硝化相对贡献率(37.1%)显著低于BC0(49.2%).生物炭施加显著增加了细菌反硝化功能基因(nirK、nirS和nosZ)的丰度,减低了fungal nirK基因的丰度.相关分析结果表明真菌反硝化贡献率与N_(2)O排放速率显著正相关,与土壤pH、TN、SOM和DOC显著负相关.生物炭可能通过提高pH和碳氮含量等来抑制反硝化真菌的生长,降低相关功能基因丰度,从而减弱了真菌反硝化过程NO还原为N_(2)O的能力,使真菌反硝化过程产生N_(2)O的贡献率显著下降,从而减少了稻田土壤反硝化N_(2)O排放.本研究有助于拓宽对稻田土壤反硝化过程的认知,并为生物炭施用调控真菌反硝化N_(2)O排放提供理论基础. 展开更多
关键词 生物炭 水稻土 氧化亚氮(N_(2)O) 真菌反硝化 反硝化
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