Chinese cabbage(Brassica rapa ssp. pekinensis) has a long cultivation history and is one of the vegetable crops with the largest cultivation area in China. However, salt stress severely damages photosynthesis and horm...Chinese cabbage(Brassica rapa ssp. pekinensis) has a long cultivation history and is one of the vegetable crops with the largest cultivation area in China. However, salt stress severely damages photosynthesis and hormone metabolism, nutritional balances, and results in ion toxicity in plants. To better understand the mechanisms of salt-induced growth inhibition in Chinese cabbage, RNA-seq and physiological index determination were conducted to explore the impacts of salt stress on carbon cycle metabolism and photosynthesis in Chinese cabbage. Here, we found that the number of thylakoids and grana lamellae and the content of starch granules and chlorophyll in the leaves of Chinese cabbage under salt stress showed a time-dependent response, first increasing and then decreasing. Chinese cabbage increased the transcript levels of genes related to the photosynthetic apparatus and carbon metabolism under salt stress, probably in an attempt to alleviate damage to the photosynthetic system and enhance CO_(2) fixation and energy metabolism. The transcription of genes related to starch and sucrose synthesis and degradation were also enhanced;this might have been an attempt to maintain intracellular osmotic pressure by increasing soluble sugar concentrations. Soluble sugars could also be used as potential reactive oxygen species(ROS) scavengers, in concert with peroxidase(POD)enzymes, to eliminate ROS that accumulate during metabolic processes. Our study characterizes the synergistic response network of carbon metabolism and photosynthesis under salt stress.展开更多
The Backscatter communication has gained widespread attention from academia and industry in recent years. In this paper, A method of resource allocation and trajectory optimization is proposed for UAV-assisted backsca...The Backscatter communication has gained widespread attention from academia and industry in recent years. In this paper, A method of resource allocation and trajectory optimization is proposed for UAV-assisted backscatter communication based on user trajectory. This paper will establish an optimization problem of jointly optimizing the UAV trajectories, UAV transmission power and BD scheduling based on the large-scale channel state signals estimated in advance of the known user trajectories, taking into account the constraints of BD data and working energy consumption, to maximize the energy efficiency of the system. The problem is a non-convex optimization problem in fractional form, and there is nonlinear coupling between optimization variables.An iterative algorithm is proposed based on Dinkelbach algorithm, block coordinate descent method and continuous convex optimization technology. First, the objective function is converted into a non-fractional programming problem based on Dinkelbach method,and then the block coordinate descent method is used to decompose the original complex problem into three independent sub-problems. Finally, the successive convex approximation method is used to solve the trajectory optimization sub-problem. The simulation results show that the proposed scheme and algorithm have obvious energy efficiency gains compared with the comparison scheme.展开更多
Protoplast-based transient gene expression system has been widely used in plant genome editing because of its simple operation and less time-consuming.In order to establish a universal protoplast-based transient trans...Protoplast-based transient gene expression system has been widely used in plant genome editing because of its simple operation and less time-consuming.In order to establish a universal protoplast-based transient transfection system for verifying activities of genome editing vectors containing targets in Brassica,we systematically optimized factors affecting protoplast isolation and transient gene expression.We established an efficient protoplast-based transient gene expression system(PTGE)in Chinese cabbage,achieving high protoplast yield of 4.9×10^(5)·g^(-1)FW,viability over 95%,and transfection efficiency of 76%.We showed for the first time that pretreatment of protoplasts with a hypotonic MMG could significantly enhance the transfection efficiency.Furthermore,protoplasts incubated at 37℃ for 6 min improved the transfection efficiency to 86%.We also demonstrated that PTGE worked well(more than 50%transfection efficiency)in multiple Brassica species including cabbage,Pak Choi,Chinese kale,and turnip.Finally,PTGE was used for validating the activities of CRISPR/Cas9 vectors containing targets in Chinese cabbage,cabbage,and pak choi,demonstrating the broad applicability of the established PTGE for genome editing in Brassica crops.展开更多
目的探究超声造影联合血清甲胎蛋白(alpha-fetoprotein,AFP)对微波消融术治疗原发性肝癌的预测价值。方法收集2020年7月至2022年8月于树兰(杭州)医院就诊的148例原发性肝癌患者,采用随机数字表法分为微波消融组和对照组,每组74例。微波...目的探究超声造影联合血清甲胎蛋白(alpha-fetoprotein,AFP)对微波消融术治疗原发性肝癌的预测价值。方法收集2020年7月至2022年8月于树兰(杭州)医院就诊的148例原发性肝癌患者,采用随机数字表法分为微波消融组和对照组,每组74例。微波消融组患者采用化疗联合微波消融术治疗,对照组患者仅采用化疗,比较两组患者的预后情况。根据微波消融组患者预后情况分为预后良好组与预后不良组,分析预后良好组与预后不良组患者超声造影微波消融灶截面积、血清AFP指标差异。采用受试者操作特征曲线下面积(area under the curve,AUC)分析诊断价值,采用Pearson法分析相关性。结果预后良好组、预后不良组、微波消融组、对照组累计生存率分别为100.00%、10.71%、49.11%、14.51%。术后6个月,预后良好组与预后不良组超声造影微波消融灶截面积均增加,且预后良好组大于预后不良组(P<0.05);预后良好组血清AFP水平下降,预后不良组水平上升(P<0.05)。超声造影微波消融灶截面积、血清AFP及联合检测预测原发性肝癌预后的AUC分别为0.760、0.740、0.833。超声造影微波消融灶截面积与死亡患者生存时间呈正相关,血清AFP与生存时间呈负相关(P<0.05)。结论超声造影联合血清AFP对原发性肝癌疗效有较好的预测价值。展开更多
基金financially supported by the Natural Science Foundation of Hebei Province-Innovation Group Research Project(Grant No.C2020204111)the National Natural Science Foundation of China(Grant No.31930098)+3 种基金the Science Fund for Distinguished Young Scholars of Hebei Province(Grant No.C2021204049)the Hebei Province Outstanding Youth Fund(Grant No.BJ2021024)the Hebei Provincial Key Research Projects(21326344D)Hebei International Joint Research Base of Modern Agricultural Biotechnology.
文摘Chinese cabbage(Brassica rapa ssp. pekinensis) has a long cultivation history and is one of the vegetable crops with the largest cultivation area in China. However, salt stress severely damages photosynthesis and hormone metabolism, nutritional balances, and results in ion toxicity in plants. To better understand the mechanisms of salt-induced growth inhibition in Chinese cabbage, RNA-seq and physiological index determination were conducted to explore the impacts of salt stress on carbon cycle metabolism and photosynthesis in Chinese cabbage. Here, we found that the number of thylakoids and grana lamellae and the content of starch granules and chlorophyll in the leaves of Chinese cabbage under salt stress showed a time-dependent response, first increasing and then decreasing. Chinese cabbage increased the transcript levels of genes related to the photosynthetic apparatus and carbon metabolism under salt stress, probably in an attempt to alleviate damage to the photosynthetic system and enhance CO_(2) fixation and energy metabolism. The transcription of genes related to starch and sucrose synthesis and degradation were also enhanced;this might have been an attempt to maintain intracellular osmotic pressure by increasing soluble sugar concentrations. Soluble sugars could also be used as potential reactive oxygen species(ROS) scavengers, in concert with peroxidase(POD)enzymes, to eliminate ROS that accumulate during metabolic processes. Our study characterizes the synergistic response network of carbon metabolism and photosynthesis under salt stress.
文摘The Backscatter communication has gained widespread attention from academia and industry in recent years. In this paper, A method of resource allocation and trajectory optimization is proposed for UAV-assisted backscatter communication based on user trajectory. This paper will establish an optimization problem of jointly optimizing the UAV trajectories, UAV transmission power and BD scheduling based on the large-scale channel state signals estimated in advance of the known user trajectories, taking into account the constraints of BD data and working energy consumption, to maximize the energy efficiency of the system. The problem is a non-convex optimization problem in fractional form, and there is nonlinear coupling between optimization variables.An iterative algorithm is proposed based on Dinkelbach algorithm, block coordinate descent method and continuous convex optimization technology. First, the objective function is converted into a non-fractional programming problem based on Dinkelbach method,and then the block coordinate descent method is used to decompose the original complex problem into three independent sub-problems. Finally, the successive convex approximation method is used to solve the trajectory optimization sub-problem. The simulation results show that the proposed scheme and algorithm have obvious energy efficiency gains compared with the comparison scheme.
基金financially supported by the Key project of National Natural Science Foundation of China (Grant No.32330096)Innovative Research Group Project of Hebei Natural Science Foundation (Grant No.C2024204246)+3 种基金S&T Program of Hebei (Grant Nos.21372901D23567601H)Natural Science Foundation of Hebei (Grant No.C2023204119)the Starting Grant from Hebei Agricultural University (Grant No.YJ201958)。
文摘Protoplast-based transient gene expression system has been widely used in plant genome editing because of its simple operation and less time-consuming.In order to establish a universal protoplast-based transient transfection system for verifying activities of genome editing vectors containing targets in Brassica,we systematically optimized factors affecting protoplast isolation and transient gene expression.We established an efficient protoplast-based transient gene expression system(PTGE)in Chinese cabbage,achieving high protoplast yield of 4.9×10^(5)·g^(-1)FW,viability over 95%,and transfection efficiency of 76%.We showed for the first time that pretreatment of protoplasts with a hypotonic MMG could significantly enhance the transfection efficiency.Furthermore,protoplasts incubated at 37℃ for 6 min improved the transfection efficiency to 86%.We also demonstrated that PTGE worked well(more than 50%transfection efficiency)in multiple Brassica species including cabbage,Pak Choi,Chinese kale,and turnip.Finally,PTGE was used for validating the activities of CRISPR/Cas9 vectors containing targets in Chinese cabbage,cabbage,and pak choi,demonstrating the broad applicability of the established PTGE for genome editing in Brassica crops.
文摘目的探究超声造影联合血清甲胎蛋白(alpha-fetoprotein,AFP)对微波消融术治疗原发性肝癌的预测价值。方法收集2020年7月至2022年8月于树兰(杭州)医院就诊的148例原发性肝癌患者,采用随机数字表法分为微波消融组和对照组,每组74例。微波消融组患者采用化疗联合微波消融术治疗,对照组患者仅采用化疗,比较两组患者的预后情况。根据微波消融组患者预后情况分为预后良好组与预后不良组,分析预后良好组与预后不良组患者超声造影微波消融灶截面积、血清AFP指标差异。采用受试者操作特征曲线下面积(area under the curve,AUC)分析诊断价值,采用Pearson法分析相关性。结果预后良好组、预后不良组、微波消融组、对照组累计生存率分别为100.00%、10.71%、49.11%、14.51%。术后6个月,预后良好组与预后不良组超声造影微波消融灶截面积均增加,且预后良好组大于预后不良组(P<0.05);预后良好组血清AFP水平下降,预后不良组水平上升(P<0.05)。超声造影微波消融灶截面积、血清AFP及联合检测预测原发性肝癌预后的AUC分别为0.760、0.740、0.833。超声造影微波消融灶截面积与死亡患者生存时间呈正相关,血清AFP与生存时间呈负相关(P<0.05)。结论超声造影联合血清AFP对原发性肝癌疗效有较好的预测价值。
