Background Cotton is an economically important crop.It is crucial to find an effective method to improve cotton yield,and one approach is to decrease the abscission of cotton bolls and buds.However,the lack of knowled...Background Cotton is an economically important crop.It is crucial to find an effective method to improve cotton yield,and one approach is to decrease the abscission of cotton bolls and buds.However,the lack of knowledge of the genetic and molecular mechanisms underlying cotton boll abscission traits has hindered genetic improvements.Results Pearson’s correlation analysis revealed a significant positive correlation between boll abscission rates 1(AR1)and boll abscission rates 2(AR2).A genome-wide association study was conducted on 145 loci that exhibited high polymorphism and were uniformly distributed across 26 chromosomes(pair).The study revealed 18,46,and 62 markers that were significantly associated with boll abscission,fiber quality,and yield traits(P<0.05),explaining 1.75%–7.13%,1.16%–9.58%,and 1.40%–5.44%of the phenotypic variation,respectively.Notably,the marker MON_SHIN-1584b was associated with the cotton boll abscission trait,whereas MON_CGR5732a was associated with cotton boll abscission and fiber quality traits.Thirteen of the marker loci identified in this study had been previously reported.Based on phenotypic effects,six typical cultivars with elite alleles related to cotton boll abscission,fiber quality,and yield traits were identified.These cultivars hold great promise for widespread utilization in breeding programs.Conclusions These results lay the foundation for understanding the molecular regulatory mechanism of cotton boll abscission and provide data for the future improvement of cotton breeding.展开更多
Background Understanding the mechanism of male sterility is crucial for producing hybrid seeds and developing sterile germplasm resources.However,only a few cytoplasmic male sterility(CMS)lines of cotton have been pro...Background Understanding the mechanism of male sterility is crucial for producing hybrid seeds and developing sterile germplasm resources.However,only a few cytoplasmic male sterility(CMS)lines of cotton have been produced due to several challenges,like inadequate variation of agronomic traits,incomplete sterility,weak resilience of restorer lines,and difficulty in combining strong dominance.Therefore,the morphological and cytological identification of CMS in cotton will facilitate hybrid breeding.Results Two F_(2) segregating populations of cotton were constructed from cytoplasmic male sterile lines(HaA and 01A,maternal)and restorer lines(HaR and 26R,paternal).Genetic analysis of these populations revealed a segregation ratio of 3:1 for fertile to sterile plants.Phenotypic analysis indicated no significant differences in traits of flower bud development between sterile and fertile plants.However,sterile plants exhibited smaller floral organs,shortened filament lengths,and anther atrophy on the flowering day in comparison with the fertile plants.When performed scanning electron microscopy(SEM),the two F_(2) populations revealed morphological variations in the anther epidermis.Cellular analysis showed no significant differences in pollen development before pollen maturation.Interestingly,between the pollen maturation and flowering stages,the tapetum layer of sterile plants degenerated prematurely,resulting in abnormal pollen grains and gradual pollen degradation.Conclusion The results of this study suggest that fertility-restoring genes are controlled by a single dominant gene.Sterile plants exhibit distinctive floral morphology,which is characterized by stamen atrophy and abnormal anthers.Pollen abortion occurs between pollen maturity and flowering,indicating that premature tapetum degradation may be the primary cause of pollen abortion.Overall,our study provides a theoretical basis for utilizing CMS in hybrid breeding and in-depth investigation of the dominant configuration of cotton hybrid combinations,mechanisms of sterility,and the role of sterile and restorer genes.展开更多
Correction:J Cotton Res 7,20(2024)https://doi.org/10.1186/s42397-024-00180-3 Following publication of the original article(Shui et al.2024),the author found 5 errors in the published article.1.One of the author’s nam...Correction:J Cotton Res 7,20(2024)https://doi.org/10.1186/s42397-024-00180-3 Following publication of the original article(Shui et al.2024),the author found 5 errors in the published article.1.One of the author’s name has been corrected from Gou Chunping to Guo Chunping.2.The reference(Zhao SQ.2016)in Table 2 has been updated to:Zhao SQ.Analysis on the major gene and multigene mixed inheritance and QTL mapping for early maturity traits in upland cotton.Chin Acad Agric Sci.2016.https://doi.org/10.3969/j.issn.201600501.(in Chinese with English abstract).3.In’Results’part,’Phenotype analysis of 238 cotton boll abscission among cotton accessions’paragraph,the phenotype analysis of AR1 ranging from 19.27%–63.79%,which was wrongly written as 19.27%-63.97%.4.The‘2018KRL’is modified to‘2018KEL’in Table 1.展开更多
Background: Cotton fiber yield is a complex trait,which can be influenced by multiple agronomic traits.Unravelling the genetic basis of cotton fiber yield-related traits contributes to genetic improvement of cotton.Re...Background: Cotton fiber yield is a complex trait,which can be influenced by multiple agronomic traits.Unravelling the genetic basis of cotton fiber yield-related traits contributes to genetic improvement of cotton.Results: In this study,503 upland cotton varieties covering the four breeding stages(BS1–BS4,1911–2011)in China were used for association mapping and domestication analysis.One hundred and forty SSR markers significantly associated with ten fiber yield-related traits were identified,among which,29 markers showed an increasing trend contribution to cotton yield-related traits from BS1 to BS4,and 26 markers showed decreased trend effect.Four favorable alleles of 9 major loci(R^(2)≥3)were strongly selected during the breeding stages,and the candidate genes of the four strongly selected alleles were predicated according to the gene function annotation and tissue expression data.Conclusions :The study not only uncovers the genetic basis of 10 cotton yield-related traits but also provides genetic evidence for cotton improvement during the cotton breeding process in China.展开更多
Background:Naturally-colored cotton has become increasingly popular because of their natural properties of coloration,UV protection,flame retardant,antibacterial activity and mildew resistance.But poor fiber quality a...Background:Naturally-colored cotton has become increasingly popular because of their natural properties of coloration,UV protection,flame retardant,antibacterial activity and mildew resistance.But poor fiber quality and limited color choices are two key issues that have restricted the cultivation of naturally-colored cotton.To identify the possible pathways participating in fiber pigmentation in naturally-colored cottons,five colored cotton accessions in three different color types(with green,brown and white fiber)were chosen for a comprehensive analysis of phenylpropanoid metabolism during fiber development.Results:The expression levels of flavonoid biosynthesis pathway genes in brown cotton fibers were significantly higher than those in white and green cotton fibers.Total flavonoids and proanthocyanidin were higher in brown cotton fibers relative to those in white and green cotton fibers,which suggested that the flavonoid biosynthesis pathway might not participate in the pigmentation of green cotton fibers.Further expression analysis indicated that the genes encoding enzymes for the synthesis of caffeic acid derivatives,lignin and lignan were activated in the developing fibers of the green cotton at 10 and 15 days post-anthesis.Conclusions:Our results strengthen the understanding of phenylpropanoid metabolism and pigmentation in green and brown cotton fibers,and may improve the breeding of naturally-colored cottons.展开更多
目的:探讨体外动态心脏模型与基于案例和问题学习(case and problem-based learning,CPBL)教学方法相结合在心脏超声科住院医师规范化培训(下文简称住培)中的应用效果。方法:采用试验对照研究方法,选取在南京医科大学附属无锡第二医院...目的:探讨体外动态心脏模型与基于案例和问题学习(case and problem-based learning,CPBL)教学方法相结合在心脏超声科住院医师规范化培训(下文简称住培)中的应用效果。方法:采用试验对照研究方法,选取在南京医科大学附属无锡第二医院心脏超声科进行住培的80名学员为研究对象。将2019年9月至2020年8月入科的40名学员设为试验组,2018年9月至2019年8月入科的40名学员设为对照组。试验组接受体外动态心脏模型与CPBL相结合的教学方法,对照组接受常规教具与CPBL相结合的教学方法。比较两组学员出科时的考核成绩,并对两组学员进行教学满意度问卷调查。结果:试验组学员的理论考试成绩、读片成绩、操作考核成绩和人文素养成绩分别为(42.72±1.78)分、(43.00±1.91)分、(44.27±2.24)分、(43.37±2.35)分均高于对照组学员的(41.82±1.75)分、(41.55±2.26)分、(43.05±2.64)分、(41.57±2.48)分,其差异均具有统计学意义(均P<0.05)。问卷调查结果显示,试验组学员比对照组学员在"加深理论知识理解"[92.5%(37/40)比75.0%(30/40)]、"培养临床思维能力"[90.0%(36/40)比70.0%(28/40)]、"提高影像读片能力"[92.5%(37/40)比72.5%(29/40)]、"提高上机操作能力"[90.0%(36/40)比67.5%(27/40)]、"提高学习兴趣"[90.0%(36/40)比72.5%(29/40)]和"提高学习效率"[92.5%(37/40)比75.0%(30/40)]方面的选择比例高,差异均具有统计学意义(均P<0.05)。结论:体外动态心脏模型与CPBL相结合的教学方法用于心脏超声科住培,能够调动住院医师的主观能动性,有助于完成住培培养目标、提高住培的教学效果。展开更多
基金Key Laboratory of Cotton Biology Open Fund(CB2022A11)National Natural Science Foundation of China(32260510)+3 种基金Innovation talent Program in Sciences and Technologies of Xinjiang Production and Construction Corps,China(2021CB028)Key Programs for Science and Technology Development of Shihezi city,Xinjiang Production and Construction Crops,China(2022NY01)Science and Technology Planning of Shuanghe city,Xinjiang Production and Construction Crops,China(2021NY02)key programs for science and technology development in agricultural field of Xinjiang Production and Construction Corps,China.
文摘Background Cotton is an economically important crop.It is crucial to find an effective method to improve cotton yield,and one approach is to decrease the abscission of cotton bolls and buds.However,the lack of knowledge of the genetic and molecular mechanisms underlying cotton boll abscission traits has hindered genetic improvements.Results Pearson’s correlation analysis revealed a significant positive correlation between boll abscission rates 1(AR1)and boll abscission rates 2(AR2).A genome-wide association study was conducted on 145 loci that exhibited high polymorphism and were uniformly distributed across 26 chromosomes(pair).The study revealed 18,46,and 62 markers that were significantly associated with boll abscission,fiber quality,and yield traits(P<0.05),explaining 1.75%–7.13%,1.16%–9.58%,and 1.40%–5.44%of the phenotypic variation,respectively.Notably,the marker MON_SHIN-1584b was associated with the cotton boll abscission trait,whereas MON_CGR5732a was associated with cotton boll abscission and fiber quality traits.Thirteen of the marker loci identified in this study had been previously reported.Based on phenotypic effects,six typical cultivars with elite alleles related to cotton boll abscission,fiber quality,and yield traits were identified.These cultivars hold great promise for widespread utilization in breeding programs.Conclusions These results lay the foundation for understanding the molecular regulatory mechanism of cotton boll abscission and provide data for the future improvement of cotton breeding.
基金supported by the Fund for the Biological Breeding-Major Projects in National Science and Technology(2023ZD04038)the Key Project for Agricultural Breakthrough in Core Technology of Xinjiang Production and Construction Crops(NYHXGG,2023AA102)the Key Project for Science and Technology Development of Shihezi city,Xinjiang Production and Construction Crops(2022NY01)。
文摘Background Understanding the mechanism of male sterility is crucial for producing hybrid seeds and developing sterile germplasm resources.However,only a few cytoplasmic male sterility(CMS)lines of cotton have been produced due to several challenges,like inadequate variation of agronomic traits,incomplete sterility,weak resilience of restorer lines,and difficulty in combining strong dominance.Therefore,the morphological and cytological identification of CMS in cotton will facilitate hybrid breeding.Results Two F_(2) segregating populations of cotton were constructed from cytoplasmic male sterile lines(HaA and 01A,maternal)and restorer lines(HaR and 26R,paternal).Genetic analysis of these populations revealed a segregation ratio of 3:1 for fertile to sterile plants.Phenotypic analysis indicated no significant differences in traits of flower bud development between sterile and fertile plants.However,sterile plants exhibited smaller floral organs,shortened filament lengths,and anther atrophy on the flowering day in comparison with the fertile plants.When performed scanning electron microscopy(SEM),the two F_(2) populations revealed morphological variations in the anther epidermis.Cellular analysis showed no significant differences in pollen development before pollen maturation.Interestingly,between the pollen maturation and flowering stages,the tapetum layer of sterile plants degenerated prematurely,resulting in abnormal pollen grains and gradual pollen degradation.Conclusion The results of this study suggest that fertility-restoring genes are controlled by a single dominant gene.Sterile plants exhibit distinctive floral morphology,which is characterized by stamen atrophy and abnormal anthers.Pollen abortion occurs between pollen maturity and flowering,indicating that premature tapetum degradation may be the primary cause of pollen abortion.Overall,our study provides a theoretical basis for utilizing CMS in hybrid breeding and in-depth investigation of the dominant configuration of cotton hybrid combinations,mechanisms of sterility,and the role of sterile and restorer genes.
文摘Correction:J Cotton Res 7,20(2024)https://doi.org/10.1186/s42397-024-00180-3 Following publication of the original article(Shui et al.2024),the author found 5 errors in the published article.1.One of the author’s name has been corrected from Gou Chunping to Guo Chunping.2.The reference(Zhao SQ.2016)in Table 2 has been updated to:Zhao SQ.Analysis on the major gene and multigene mixed inheritance and QTL mapping for early maturity traits in upland cotton.Chin Acad Agric Sci.2016.https://doi.org/10.3969/j.issn.201600501.(in Chinese with English abstract).3.In’Results’part,’Phenotype analysis of 238 cotton boll abscission among cotton accessions’paragraph,the phenotype analysis of AR1 ranging from 19.27%–63.79%,which was wrongly written as 19.27%-63.97%.4.The‘2018KRL’is modified to‘2018KEL’in Table 1.
基金This work was supported by the National Natural Science Foundation of China(31760402)Young and Middle-aged Science and Technology Leading Talents of Xinjiang Production and Construction Corps(2019CB027).
文摘Background: Cotton fiber yield is a complex trait,which can be influenced by multiple agronomic traits.Unravelling the genetic basis of cotton fiber yield-related traits contributes to genetic improvement of cotton.Results: In this study,503 upland cotton varieties covering the four breeding stages(BS1–BS4,1911–2011)in China were used for association mapping and domestication analysis.One hundred and forty SSR markers significantly associated with ten fiber yield-related traits were identified,among which,29 markers showed an increasing trend contribution to cotton yield-related traits from BS1 to BS4,and 26 markers showed decreased trend effect.Four favorable alleles of 9 major loci(R^(2)≥3)were strongly selected during the breeding stages,and the candidate genes of the four strongly selected alleles were predicated according to the gene function annotation and tissue expression data.Conclusions :The study not only uncovers the genetic basis of 10 cotton yield-related traits but also provides genetic evidence for cotton improvement during the cotton breeding process in China.
基金This work was financially supported by the National Natural Science Foundation of China(31471540)National Transgenic Plant Research of China(2016ZX08005–001)to Zhang XL+3 种基金This project was also supported by the Fundamental Research Funds for the Central Universities(2662017JC030)Funding was also provided by Science and Technology Innovation Talent program(2020CB017)Scientific and Technological Breakthrough and Achievement Transformation Projects of Xinjiang Production and Construction Corps(2016 AC027)Scientific Research Project of Shihezi(2016HZ09)to You CY.
文摘Background:Naturally-colored cotton has become increasingly popular because of their natural properties of coloration,UV protection,flame retardant,antibacterial activity and mildew resistance.But poor fiber quality and limited color choices are two key issues that have restricted the cultivation of naturally-colored cotton.To identify the possible pathways participating in fiber pigmentation in naturally-colored cottons,five colored cotton accessions in three different color types(with green,brown and white fiber)were chosen for a comprehensive analysis of phenylpropanoid metabolism during fiber development.Results:The expression levels of flavonoid biosynthesis pathway genes in brown cotton fibers were significantly higher than those in white and green cotton fibers.Total flavonoids and proanthocyanidin were higher in brown cotton fibers relative to those in white and green cotton fibers,which suggested that the flavonoid biosynthesis pathway might not participate in the pigmentation of green cotton fibers.Further expression analysis indicated that the genes encoding enzymes for the synthesis of caffeic acid derivatives,lignin and lignan were activated in the developing fibers of the green cotton at 10 and 15 days post-anthesis.Conclusions:Our results strengthen the understanding of phenylpropanoid metabolism and pigmentation in green and brown cotton fibers,and may improve the breeding of naturally-colored cottons.
文摘目的:探讨体外动态心脏模型与基于案例和问题学习(case and problem-based learning,CPBL)教学方法相结合在心脏超声科住院医师规范化培训(下文简称住培)中的应用效果。方法:采用试验对照研究方法,选取在南京医科大学附属无锡第二医院心脏超声科进行住培的80名学员为研究对象。将2019年9月至2020年8月入科的40名学员设为试验组,2018年9月至2019年8月入科的40名学员设为对照组。试验组接受体外动态心脏模型与CPBL相结合的教学方法,对照组接受常规教具与CPBL相结合的教学方法。比较两组学员出科时的考核成绩,并对两组学员进行教学满意度问卷调查。结果:试验组学员的理论考试成绩、读片成绩、操作考核成绩和人文素养成绩分别为(42.72±1.78)分、(43.00±1.91)分、(44.27±2.24)分、(43.37±2.35)分均高于对照组学员的(41.82±1.75)分、(41.55±2.26)分、(43.05±2.64)分、(41.57±2.48)分,其差异均具有统计学意义(均P<0.05)。问卷调查结果显示,试验组学员比对照组学员在"加深理论知识理解"[92.5%(37/40)比75.0%(30/40)]、"培养临床思维能力"[90.0%(36/40)比70.0%(28/40)]、"提高影像读片能力"[92.5%(37/40)比72.5%(29/40)]、"提高上机操作能力"[90.0%(36/40)比67.5%(27/40)]、"提高学习兴趣"[90.0%(36/40)比72.5%(29/40)]和"提高学习效率"[92.5%(37/40)比75.0%(30/40)]方面的选择比例高,差异均具有统计学意义(均P<0.05)。结论:体外动态心脏模型与CPBL相结合的教学方法用于心脏超声科住培,能够调动住院医师的主观能动性,有助于完成住培培养目标、提高住培的教学效果。
