目的探讨人乳头状瘤病毒(HPV)与鼻腔鼻窦内翻性乳头状瘤(SNIP)发生、复发及恶变的关系。方法检索Pubmed、Web of science、Embase、Cochrane Library、CNKI、维普、中国知网及万方等数据库,查找2000~2017年国内外公开发表的HPV与SNIP发...目的探讨人乳头状瘤病毒(HPV)与鼻腔鼻窦内翻性乳头状瘤(SNIP)发生、复发及恶变的关系。方法检索Pubmed、Web of science、Embase、Cochrane Library、CNKI、维普、中国知网及万方等数据库,查找2000~2017年国内外公开发表的HPV与SNIP发生、复发及恶变相关的所有病例对照研究文献,应用RevMan 5.3统计软件对各研究原始数据进行统计分析。结果 Meta分析示SNIP组HPV感染率明显高于对照组(P<0.01,OR=19.72,95%CI为7.91~49.18);复发组HPV感染率明显高于对照组(P<0.01,OR=3.10,95%CI为1.90~5.08);恶变组HPV感染率明显高于对照组(P<0.01,OR=2.09,95%CI为1.41~3.09)。结论 HPV感染与SNIP的发生、复发及恶变密切相关。展开更多
A method for the determination of human immunoglobulin G（IgG） based on a colloidal gold label by fluorospectrophotometry was developed. The sandwich immunoreaction among goat-anti-human IgG, human IgG and goat-anti-...A method for the determination of human immunoglobulin G（IgG） based on a colloidal gold label by fluorospectrophotometry was developed. The sandwich immunoreaction among goat-anti-human IgG, human IgG and goat-anti-human IgG labeled with colloidal gold nanoparticles was applied in this experiment. First, a sandwich im- munocomplex was formed on the surface of 96 well clear polystyrene high bind stripwellTM microplate. After the formation of the sandwich immunocomplex, a solution was added to dissociate the immunocomplex at room temperature. Then the solution of each well was transferred into the corresponding test tube. Thirdly, the rhodamine B solution was injected into each test tube. The rhodamine B chloraurate was extracted into ether that was measured with a spectrofluorometer. The experimental results indicate that the fluorescence intensity increased with the increase of human IgG concentration. The fluorescence intensity of rhodamine B chloraurate at 570 nm was proportional to the logarithm of human IgG concentration in a range from 10 to 5 × 10^5 ng/mL. It was shown that the determination of human IgG was easily made with the proposed fluorospectrophotometry.展开更多
Be able to form large particles with protein via electrostatic force to enhance the intensity of resonance light scattering（RLS）,（NH4）2SO4 was used as a RLS probe to determine bovine serum albumin（BSA）,human ser...Be able to form large particles with protein via electrostatic force to enhance the intensity of resonance light scattering（RLS）,（NH4）2SO4 was used as a RLS probe to determine bovine serum albumin（BSA）,human serum albumin（HSA） and ovum albumin（OVA）.The experimental conditions were investigated,including the acidity and saturation degree of （NH4）2SO4.Under the optimum conditions,the enhanced RLS intensity is proportional to the concentration of BSA,HSA and OVA in the ranges of 5×10-8―1×10-6,2.5×10-8―8×10-7 and 5×10-8―1.5×10-6 mol/L,respectively.The detection limits for BSA,HAS and OVA are 6.6×10-9,3.8×10-9 and 7.4×10-9 mol/L,respectively.The effects of foreign substances were also examined.The practical and synthetic samples were analyzed with satisfactory results.展开更多
he electrochemistry of cytochrome C was investigated at a spectrographicgraphite electrode. In phosphate buffer solution (pH= 7. 0) , cytochrome C showedstable and quasi-reversible response. The formal potential E￣(o...he electrochemistry of cytochrome C was investigated at a spectrographicgraphite electrode. In phosphate buffer solution (pH= 7. 0) , cytochrome C showedstable and quasi-reversible response. The formal potential E￣(o') was 0. 015 V (at25℃ , vs. SCE) and the heterogeneous electron transfer rate constant k_s obtainedvaried form 1. 10×10￣(-3) cm · s￣(-1) to 1. 80k×10￣(-3) cm · s￣(-1). The thermodynamic pa-rameters of the electron transfer reaction of cvtochrome C was also estimated. Fur-thermore, the effect of the various electrode surface states on the electrochemistryof cytochrome C was discussed.展开更多
Homogeneous ionic liquid microextraction was developed for the simultaneceus extraction of dimetho- morph, mefenacet, isoprothiolane and oxadiazon from soil. 1-Butyl-3-methylimidazolium tetrafluoroborate was used as e...Homogeneous ionic liquid microextraction was developed for the simultaneceus extraction of dimetho- morph, mefenacet, isoprothiolane and oxadiazon from soil. 1-Butyl-3-methylimidazolium tetrafluoroborate was used as extraction solvent, and ammonium hexafluorophosphate was used as ion-pairing agent. High-performance liquid chromatography（HPLC） was employed for separation and determination of the analytes. The calibration curves show good linear relationship（r〉0.9988）. The recoveries are between 74.2% and 97.9% with relative standard devia- tions（RSDs） lower than 5.97%. The present method is free of volatile organic solvents, and expenditures of sample, extraction time and solvent are lower, compared with ultrasonic and Soxhlet extraction. There was no obvious diffe- rence in the extraction recoveries of pesticides obtained by the three extraction methods.展开更多
The authors performed ionic liquid-based microwave-assisted liquid-liquid microextraction（IL-based MALLME） coupled with high performance liquid chromatographic separation for the determination of 6 sulfonamides （SA...The authors performed ionic liquid-based microwave-assisted liquid-liquid microextraction（IL-based MALLME） coupled with high performance liquid chromatographic separation for the determination of 6 sulfonamides （SAs） from animal oils. The target analytes were extracted from animal oil samples with sodium hydroxide solution containing 1-butyl-3-methylimidazolium tetrafluoroborateand as the extraction solvent under microwave irradiation. The experimental parameters of the IL-based MALLME, including types of ILs, volume of IL, amount of ion-pairing agent（NHaPF6）, pH value of sample solution, and extraction temperature and time were evaluated. The limits of detection and quantification obtained were in a range of 0.4--0.5μg/kg and a range of 1.2--1.8μg/kg, respectively. The accuracy of the method was evaluated by analyzing five spiked animal oil samples at two fortified levels（5 and 50μg/kg）, and the recoveries of SAs varied from 81.4% to 114.5% with relative standard deviations ranging from 0.8% to 9.0%.展开更多
文摘A method for the determination of human immunoglobulin G（IgG） based on a colloidal gold label by fluorospectrophotometry was developed. The sandwich immunoreaction among goat-anti-human IgG, human IgG and goat-anti-human IgG labeled with colloidal gold nanoparticles was applied in this experiment. First, a sandwich im- munocomplex was formed on the surface of 96 well clear polystyrene high bind stripwellTM microplate. After the formation of the sandwich immunocomplex, a solution was added to dissociate the immunocomplex at room temperature. Then the solution of each well was transferred into the corresponding test tube. Thirdly, the rhodamine B solution was injected into each test tube. The rhodamine B chloraurate was extracted into ether that was measured with a spectrofluorometer. The experimental results indicate that the fluorescence intensity increased with the increase of human IgG concentration. The fluorescence intensity of rhodamine B chloraurate at 570 nm was proportional to the logarithm of human IgG concentration in a range from 10 to 5 × 10^5 ng/mL. It was shown that the determination of human IgG was easily made with the proposed fluorospectrophotometry.
文摘Be able to form large particles with protein via electrostatic force to enhance the intensity of resonance light scattering（RLS）,（NH4）2SO4 was used as a RLS probe to determine bovine serum albumin（BSA）,human serum albumin（HSA） and ovum albumin（OVA）.The experimental conditions were investigated,including the acidity and saturation degree of （NH4）2SO4.Under the optimum conditions,the enhanced RLS intensity is proportional to the concentration of BSA,HSA and OVA in the ranges of 5×10-8―1×10-6,2.5×10-8―8×10-7 and 5×10-8―1.5×10-6 mol/L,respectively.The detection limits for BSA,HAS and OVA are 6.6×10-9,3.8×10-9 and 7.4×10-9 mol/L,respectively.The effects of foreign substances were also examined.The practical and synthetic samples were analyzed with satisfactory results.
文摘he electrochemistry of cytochrome C was investigated at a spectrographicgraphite electrode. In phosphate buffer solution (pH= 7. 0) , cytochrome C showedstable and quasi-reversible response. The formal potential E￣(o') was 0. 015 V (at25℃ , vs. SCE) and the heterogeneous electron transfer rate constant k_s obtainedvaried form 1. 10×10￣(-3) cm · s￣(-1) to 1. 80k×10￣(-3) cm · s￣(-1). The thermodynamic pa-rameters of the electron transfer reaction of cvtochrome C was also estimated. Fur-thermore, the effect of the various electrode surface states on the electrochemistryof cytochrome C was discussed.
基金Supported by the National Natural Science Foundation of China（No.51209024）.
文摘Homogeneous ionic liquid microextraction was developed for the simultaneceus extraction of dimetho- morph, mefenacet, isoprothiolane and oxadiazon from soil. 1-Butyl-3-methylimidazolium tetrafluoroborate was used as extraction solvent, and ammonium hexafluorophosphate was used as ion-pairing agent. High-performance liquid chromatography（HPLC） was employed for separation and determination of the analytes. The calibration curves show good linear relationship（r〉0.9988）. The recoveries are between 74.2% and 97.9% with relative standard devia- tions（RSDs） lower than 5.97%. The present method is free of volatile organic solvents, and expenditures of sample, extraction time and solvent are lower, compared with ultrasonic and Soxhlet extraction. There was no obvious diffe- rence in the extraction recoveries of pesticides obtained by the three extraction methods.
文摘The authors performed ionic liquid-based microwave-assisted liquid-liquid microextraction（IL-based MALLME） coupled with high performance liquid chromatographic separation for the determination of 6 sulfonamides （SAs） from animal oils. The target analytes were extracted from animal oil samples with sodium hydroxide solution containing 1-butyl-3-methylimidazolium tetrafluoroborateand as the extraction solvent under microwave irradiation. The experimental parameters of the IL-based MALLME, including types of ILs, volume of IL, amount of ion-pairing agent（NHaPF6）, pH value of sample solution, and extraction temperature and time were evaluated. The limits of detection and quantification obtained were in a range of 0.4--0.5μg/kg and a range of 1.2--1.8μg/kg, respectively. The accuracy of the method was evaluated by analyzing five spiked animal oil samples at two fortified levels（5 and 50μg/kg）, and the recoveries of SAs varied from 81.4% to 114.5% with relative standard deviations ranging from 0.8% to 9.0%.
