Objective A high sodium(HS)diet is believed to affect bone metabolism processes.Clarifying its impact on osseointegration of titanium(Ti)implants holds significant implications for postoperative dietary management of ...Objective A high sodium(HS)diet is believed to affect bone metabolism processes.Clarifying its impact on osseointegration of titanium(Ti)implants holds significant implications for postoperative dietary management of implanted patients.Methods This investigation probed the impact of sodium ions(Na^(+))on neovascularization and osteogenesis around Ti implants in vivo,utilizing micro-computed tomography,hematoxylin and eosin staining,and immunohistochemical analyses.Concurrently,in vitro experiments assessed the effects of varied Na^(+)concentrations and exposure durations on human umbilical vein endothelial cells(HUVECs)and MC3T3-E1 cells.Results In vivo,increased dietary sodium(0.8%-6.0%)led to a substantial decline in CD34 positive HUVECs and new bone formation around Ti implants,alongside an increase in inflammatory cells.In vitro,an increase in Na^(+)concentration(140-150 mmol/L)adversely affected the proliferation,angiogenesis,and migration of HUVECs,especially with prolonged exposure.While MC3T3-E1 cells initially exhibited less susceptibility to high Na^(+)concentrations compared to HUVECs during short-term exposure,prolonged exposure to a HS environment progressively diminished their proliferation,differentiation,and osteogenic capabilities.Conclusion These findings suggest that HS diet had a negative effect on the early osseointegration of Ti implants by interfering with the process of postoperative vascularized bone regeneration.展开更多
Previous studies have shown that proliferation of endogenous neural precursor cells cannot alone compensate for the damage to neurons and axons. From the perspective of neural plastici- ty, we observed the effects of ...Previous studies have shown that proliferation of endogenous neural precursor cells cannot alone compensate for the damage to neurons and axons. From the perspective of neural plastici- ty, we observed the effects of functional electrical stimulation treatment on endogenous neural precursor cell proliferation and expression of basic fibroblast growth factor and epidermal growth factor in the rat brain on the infarct side. Functional electrical stimulation was performed in rat models of acute middle cerebral artery occlusion. Simultaneously, we set up a placebo stimulation group and a sham-operated group. Immunohistochemical staining showed that, at 7 and 14 days, compared with the placebo group, the numbers of nestin (a neural precursor cell marker)-positive cells in the subgranular zone and subventricular zone were increased in the functional electrical stimulation treatment group. Western blot assays and reverse-transcription PCR showed that total protein levels and gene expression of epidermal growth factor and basic fibroblast growth factor were also upregulated on the infarct side. Prehensile traction test results showed that, at 14 days, prehension function of rats in the functional electrical stimulation group was significantly better than in the placebo group. These results suggest that functional electrical stimulation can promote endogenous neural precursor cell proliferation in the brains of acute cerebral infarction rats, enhance expression of basic fibroblast growth factor and epidermal growth factor, and improve the motor function of rats.展开更多
基于新的全球表面温度数据集CMST(China merged surface temperature),全面评估了参加国际耦合模式比较计划第5阶段(CMIP5)的27个全球气候模式1900—2017年的气候模拟结果(1900—2005年为模式历史模拟,2006—2017年为不同典型浓度路径...基于新的全球表面温度数据集CMST(China merged surface temperature),全面评估了参加国际耦合模式比较计划第5阶段(CMIP5)的27个全球气候模式1900—2017年的气候模拟结果(1900—2005年为模式历史模拟,2006—2017年为不同典型浓度路径下的预估)。泰勒图及各种统计参数的对比表明,一些模式无论在历史模拟时段,还是在历史模拟和近期预估拼接时段,都稳定、较好地模拟出了观测序列的变化特征。利用筛选出模拟效果相对较优的9个模式,系统比较了其集合平均MT9(mean model top 9)与所有模式的集合平均MAM(mean all models)。分析结果表明:无论在哪种排放路径下,不管是时间变化,还是从空间分布方面,多数模式可能高估了亚洲区域增暖趋势,导致MAM过高估计了亚洲区域温度变化幅度与长期趋势,而优选的模式集合MT9明显比MAM更接近于观测值。进一步,采用了MT9的预估结果分析了2018—2099年的亚洲区域预估的地表升温幅度:到2099年,在RCP2.6浓度路径下,MT9预估亚洲地区的升温幅度较小,约为0.08℃;在RCP4.5浓度路径下,升温约为1.20℃;在RCP8.5浓度路径下,升温将达3.54℃,这些结果均略小于所有模式集合MAM的升温幅度,因而更加合理;同时还基于MT9预估分析了2018—2099年的温度距平的空间变化。展开更多
The quantity of soil microbes and the structure of ammonium oxidizing bacterial (AOB) community were analyzed using the dilution plate counting and most probable number method (MPN), and denaturing gradient gel el...The quantity of soil microbes and the structure of ammonium oxidizing bacterial (AOB) community were analyzed using the dilution plate counting and most probable number method (MPN), and denaturing gradient gel electrophoresis (DGGE), respectively. Fertilizer application tended to increase the number of soil microbes and alter the AOB community compared to the control with no fertilizer application (CK). Among the eight fertilizer treatments, soil samples from the treatments of mineral fertilizers (e.g., N, P, K) in combination with farmyard manure (M) had greater number.s of soil microbes and more complex structure of AOB community than those receiving mineral fertilizers alone. The principal component analyses (PCA) for ammonium oxidizing bacterial community structure showed that the eight fertilizer treatments could be divided into two PCA groups (PCA1 and PCA2). For the soil sampled after rice harvest, PCA1 included NP, NM, NPM and NPKM fertilizer treatments, while PCA2 was consisted of CK, N, M and NPK fertilizer treatments. For soil samples collected after wheat harvest, PCA1 was consisted of M, NM, NPM and NPKM fertilizer treatments, while PCA2 was composed of CK, N, NP and NPK fertilizer treatments. For a given rotation, the richness of AOB community in PCA1 was greater than that in PCA2. In addition, AOB community structure was more complex in the soil after rice harvest than that after wheat harvest. The results indicated that different fertilizer treatments resulted in substantial changes of soil microbe number and AOB community. Furthermore, mineral fertilizers (N, NP, NPK) combined with farmyard manure were effective for increasing the quantity of soil microbes, enriching AOB community, and improving the soil biofertility.展开更多
The Measures on Aid and Management for Urban Vagrants and Beggars, issued by the State Council in mid-July, took effect on August 1. It marks a further improvement of China’s mechanism to aid urban vagrants and begga...The Measures on Aid and Management for Urban Vagrants and Beggars, issued by the State Council in mid-July, took effect on August 1. It marks a further improvement of China’s mechanism to aid urban vagrants and beggars. The new regulation has been enacted to replace the two-decade-old Measures for the Internment and Deportation of Urban Vagrants and Beggars. This policy reform has stemmed from the astounding case of Sun Zhigang, a young man that was brutally beaten to death while in custody at the Guangzhou Detention Center.展开更多
Farmer workers are a new powerful workforce that has sprung up amid China's reform and door opening and in the process of the country's industrialization and urbanization. They have made great contributions to urban...Farmer workers are a new powerful workforce that has sprung up amid China's reform and door opening and in the process of the country's industrialization and urbanization. They have made great contributions to urban prosperity, rural development and their country's modernization. At present, however, farmer workers face numerous difficulties and problems in income, work safety, social security, vocational training, the schooling of their children and living conditions. To safeguard the legitimate rights and interests of farmer workers, the State Council in March 2006 issued Ideas of the State Council on Solving Farmer Workers-related Problems. In the document, the State Council promises to strengthen and improve leadership over work regarding farmer workers and prioritize the solving of farmer workers-related problems.展开更多
Lu Xueyi graduated from the Philosophy Department of Beijing University in 1962. He pursued graduate studies at the Philosophy Institute of the Chinese Academy of Social Sciences during 1962-1965. He has long been eng...Lu Xueyi graduated from the Philosophy Department of Beijing University in 1962. He pursued graduate studies at the Philosophy Institute of the Chinese Academy of Social Sciences during 1962-1965. He has long been engaged in the study of sociology, social structure and rural development. He worked once as director of the Sociology Institute of CASS. After 1998 he.. has served as member of the Academic Committee of CASS. In 1996 he became president of China Sociology Society. He was a deputy to the Eighth and Ninth National People's Congresses. He is author of The Chinese Countryside and Chinese Farmers amid Reforms, Chinese Society in a Transitional Period, Evolution of China's Social Structure and On Agriculture, the Countryside and Peasantry.展开更多
A root hair is a polarly elongated single-celled structure that derives from a root epidermal cell and func-tions in uptake of water and nutrients from the surrounding environment.Previous reports have demon-strated t...A root hair is a polarly elongated single-celled structure that derives from a root epidermal cell and func-tions in uptake of water and nutrients from the surrounding environment.Previous reports have demon-strated that short periods of high pH inhibit root hair extension;but the effects of long-term high-pH treat-ment on root hair growth are still unclear.Here,we report that the duration of root hair elongation is signicantly prolonged with increasing external pH,which counteracts the effect of decreasing root hair elongation rate and ultimately produces longer root hairs,whereas loss of actin-depolymerizing factor 8 and 11(ADF8/11)function causes shortening of root hair length at high pH(pH 7.4).Accumulation of ADF8/11 at the tips of root hairs is inhibited by high pH,and increasing environmental pH affects the actinlament(F-actin)meshwork at the root hair tip.At high pH,the tip-focused F-actin meshwork is absent in root hairs of the adf8/11 mutant,actinlaments are disordered at the adf8/11 root hair tips,and actin turn-over is attenuated.Secretory and recycling vesicles do not aggregate in the apical region of adf8/11 root hairs at high pH.Together,our results suggest that,under long-term exposure to high extracellular pH,ADF8/11 may establish and maintain the tip-focused F-actin meshwork to regulate polar trafcking of secretory/recycling vesicles at the root hair tips,thereby promoting root hair elongation.展开更多
Background:The pathogenesis of multiple sclerosis(MS)and experimental autoimmune encephalomyelitis(EAE-an animal model of MS)is primarily mediated by T cells.However,recent studies have only focused on interleukin(IL)...Background:The pathogenesis of multiple sclerosis(MS)and experimental autoimmune encephalomyelitis(EAE-an animal model of MS)is primarily mediated by T cells.However,recent studies have only focused on interleukin(IL)-17-secreting CD4^(+)T-helper cells,also known as Th17 cells.This study aimed to compare Th17 cells and IL-17-secreting CD8^(+)T-cytotoxic cells(Tc17)in the context of MS/EAE.Methods:Female C57BL/6 mice were immunized with myelin oligodendrocyte glycoprotein peptides 35-55(MOG35-55),pertussis toxin,and complete Freund's adjuvant to establish the EAE animal model.T cells were isolated from the spleen(12-14 days postimmunization).CD4^(+)and CD8^(+)T cells were purified using isolation kit and then differentiated into Th17 and Tc17,respectively,using MOG35-55 and IL-23.The secretion levels of interferon-(IFN-γ)and IL-17 were measured via enzyme-linked immunosorbent assay using cultured CD4^(+)and CD8^(+)T cell supernatants.The pathogenicity of Tc17 and Th17 cells was assessed through adoptive transfer(tEAE),with the clinical course assessed using an EAE score(0-5).Hematoxylin and eosin as well as Luxol fast blue staining were used to examine the spinal cord.Purified CD8^(+)CD3^(+)and CD4^(+)CD3^(+)cells differentiated into Tc17 and Th17 cells,respectively,were stimulated with MOG35-55 peptide for proliferation assays.Results:The results showed that Tc17 cells(15,951±19855VS.55,709±4196cpm;p<0.050)exhibited a weaker response to highest dose(20μg/mL)MOG35-55 than Th17 cells.However,this response was not dependent on Th17 cells.After the 48h stimulation,at the highest dose(20μg/mL)of MOG35-55.Tc17 cells secreted lower levels of IFN-(280.00±15.00vs.556.67±15.28pg/mL,p<0.050)and IL-17(102.67±5.86 pg/mL vs.288.33±12.58 pg/mL;p<0.050)than Th17 cells.Similar patterns were observed for IFN-γsecretion at 96 and 144h.Furthermore,Tc17 cell-induced tEAE mice exhibited similar EAE scores to Th17 cell-induced tEAE mice and also showed similar inflammation and demyelination.Conclusion:The degree of pathogenicity of Tc17 cells in EAE is lower than that of Th17 cells.Future investigation on different immune cells and EAE models is warranted to determine the mechanisms underlying MS.展开更多
基金funded by the Wenzhou Public Welfare Science and Technology Project(Y2020118)Zhejiang Provincial Science and Technology Project for Public Welfare(LQ23H140001)Wenzhou Medical University Basic Scientific Research Operating Expenses(KYYW202230).
文摘Objective A high sodium(HS)diet is believed to affect bone metabolism processes.Clarifying its impact on osseointegration of titanium(Ti)implants holds significant implications for postoperative dietary management of implanted patients.Methods This investigation probed the impact of sodium ions(Na^(+))on neovascularization and osteogenesis around Ti implants in vivo,utilizing micro-computed tomography,hematoxylin and eosin staining,and immunohistochemical analyses.Concurrently,in vitro experiments assessed the effects of varied Na^(+)concentrations and exposure durations on human umbilical vein endothelial cells(HUVECs)and MC3T3-E1 cells.Results In vivo,increased dietary sodium(0.8%-6.0%)led to a substantial decline in CD34 positive HUVECs and new bone formation around Ti implants,alongside an increase in inflammatory cells.In vitro,an increase in Na^(+)concentration(140-150 mmol/L)adversely affected the proliferation,angiogenesis,and migration of HUVECs,especially with prolonged exposure.While MC3T3-E1 cells initially exhibited less susceptibility to high Na^(+)concentrations compared to HUVECs during short-term exposure,prolonged exposure to a HS environment progressively diminished their proliferation,differentiation,and osteogenic capabilities.Conclusion These findings suggest that HS diet had a negative effect on the early osseointegration of Ti implants by interfering with the process of postoperative vascularized bone regeneration.
基金the National Natural Science Foundation of China,grants No.30772304,30973166,and 81171863
文摘Previous studies have shown that proliferation of endogenous neural precursor cells cannot alone compensate for the damage to neurons and axons. From the perspective of neural plastici- ty, we observed the effects of functional electrical stimulation treatment on endogenous neural precursor cell proliferation and expression of basic fibroblast growth factor and epidermal growth factor in the rat brain on the infarct side. Functional electrical stimulation was performed in rat models of acute middle cerebral artery occlusion. Simultaneously, we set up a placebo stimulation group and a sham-operated group. Immunohistochemical staining showed that, at 7 and 14 days, compared with the placebo group, the numbers of nestin (a neural precursor cell marker)-positive cells in the subgranular zone and subventricular zone were increased in the functional electrical stimulation treatment group. Western blot assays and reverse-transcription PCR showed that total protein levels and gene expression of epidermal growth factor and basic fibroblast growth factor were also upregulated on the infarct side. Prehensile traction test results showed that, at 14 days, prehension function of rats in the functional electrical stimulation group was significantly better than in the placebo group. These results suggest that functional electrical stimulation can promote endogenous neural precursor cell proliferation in the brains of acute cerebral infarction rats, enhance expression of basic fibroblast growth factor and epidermal growth factor, and improve the motor function of rats.
文摘基于新的全球表面温度数据集CMST(China merged surface temperature),全面评估了参加国际耦合模式比较计划第5阶段(CMIP5)的27个全球气候模式1900—2017年的气候模拟结果(1900—2005年为模式历史模拟,2006—2017年为不同典型浓度路径下的预估)。泰勒图及各种统计参数的对比表明,一些模式无论在历史模拟时段,还是在历史模拟和近期预估拼接时段,都稳定、较好地模拟出了观测序列的变化特征。利用筛选出模拟效果相对较优的9个模式,系统比较了其集合平均MT9(mean model top 9)与所有模式的集合平均MAM(mean all models)。分析结果表明:无论在哪种排放路径下,不管是时间变化,还是从空间分布方面,多数模式可能高估了亚洲区域增暖趋势,导致MAM过高估计了亚洲区域温度变化幅度与长期趋势,而优选的模式集合MT9明显比MAM更接近于观测值。进一步,采用了MT9的预估结果分析了2018—2099年的亚洲区域预估的地表升温幅度:到2099年,在RCP2.6浓度路径下,MT9预估亚洲地区的升温幅度较小,约为0.08℃;在RCP4.5浓度路径下,升温约为1.20℃;在RCP8.5浓度路径下,升温将达3.54℃,这些结果均略小于所有模式集合MAM的升温幅度,因而更加合理;同时还基于MT9预估分析了2018—2099年的温度距平的空间变化。
基金the National Key Technology R&D Program of China (2006BAD05B06 and 2006BAD 02A14-13)
文摘The quantity of soil microbes and the structure of ammonium oxidizing bacterial (AOB) community were analyzed using the dilution plate counting and most probable number method (MPN), and denaturing gradient gel electrophoresis (DGGE), respectively. Fertilizer application tended to increase the number of soil microbes and alter the AOB community compared to the control with no fertilizer application (CK). Among the eight fertilizer treatments, soil samples from the treatments of mineral fertilizers (e.g., N, P, K) in combination with farmyard manure (M) had greater number.s of soil microbes and more complex structure of AOB community than those receiving mineral fertilizers alone. The principal component analyses (PCA) for ammonium oxidizing bacterial community structure showed that the eight fertilizer treatments could be divided into two PCA groups (PCA1 and PCA2). For the soil sampled after rice harvest, PCA1 included NP, NM, NPM and NPKM fertilizer treatments, while PCA2 was consisted of CK, N, M and NPK fertilizer treatments. For soil samples collected after wheat harvest, PCA1 was consisted of M, NM, NPM and NPKM fertilizer treatments, while PCA2 was composed of CK, N, NP and NPK fertilizer treatments. For a given rotation, the richness of AOB community in PCA1 was greater than that in PCA2. In addition, AOB community structure was more complex in the soil after rice harvest than that after wheat harvest. The results indicated that different fertilizer treatments resulted in substantial changes of soil microbe number and AOB community. Furthermore, mineral fertilizers (N, NP, NPK) combined with farmyard manure were effective for increasing the quantity of soil microbes, enriching AOB community, and improving the soil biofertility.
文摘The Measures on Aid and Management for Urban Vagrants and Beggars, issued by the State Council in mid-July, took effect on August 1. It marks a further improvement of China’s mechanism to aid urban vagrants and beggars. The new regulation has been enacted to replace the two-decade-old Measures for the Internment and Deportation of Urban Vagrants and Beggars. This policy reform has stemmed from the astounding case of Sun Zhigang, a young man that was brutally beaten to death while in custody at the Guangzhou Detention Center.
文摘Farmer workers are a new powerful workforce that has sprung up amid China's reform and door opening and in the process of the country's industrialization and urbanization. They have made great contributions to urban prosperity, rural development and their country's modernization. At present, however, farmer workers face numerous difficulties and problems in income, work safety, social security, vocational training, the schooling of their children and living conditions. To safeguard the legitimate rights and interests of farmer workers, the State Council in March 2006 issued Ideas of the State Council on Solving Farmer Workers-related Problems. In the document, the State Council promises to strengthen and improve leadership over work regarding farmer workers and prioritize the solving of farmer workers-related problems.
文摘Lu Xueyi graduated from the Philosophy Department of Beijing University in 1962. He pursued graduate studies at the Philosophy Institute of the Chinese Academy of Social Sciences during 1962-1965. He has long been engaged in the study of sociology, social structure and rural development. He worked once as director of the Sociology Institute of CASS. After 1998 he.. has served as member of the Academic Committee of CASS. In 1996 he became president of China Sociology Society. He was a deputy to the Eighth and Ninth National People's Congresses. He is author of The Chinese Countryside and Chinese Farmers amid Reforms, Chinese Society in a Transitional Period, Evolution of China's Social Structure and On Agriculture, the Countryside and Peasantry.
基金supported by the National Natural Science Foundation of China (grants 31970195,31700161,32170331,32170330,and 6232300303)Hainan Yazhou Bay Seed Lab grant B23YQ1510,China Postdoctoral Science Foundation grant 2022M720058the Fundamental Research Funds for the Central Universities grants lzujbky-2022-ey06,lzujbky-2023-I02 and lzuibky-2023-pd10.
文摘A root hair is a polarly elongated single-celled structure that derives from a root epidermal cell and func-tions in uptake of water and nutrients from the surrounding environment.Previous reports have demon-strated that short periods of high pH inhibit root hair extension;but the effects of long-term high-pH treat-ment on root hair growth are still unclear.Here,we report that the duration of root hair elongation is signicantly prolonged with increasing external pH,which counteracts the effect of decreasing root hair elongation rate and ultimately produces longer root hairs,whereas loss of actin-depolymerizing factor 8 and 11(ADF8/11)function causes shortening of root hair length at high pH(pH 7.4).Accumulation of ADF8/11 at the tips of root hairs is inhibited by high pH,and increasing environmental pH affects the actinlament(F-actin)meshwork at the root hair tip.At high pH,the tip-focused F-actin meshwork is absent in root hairs of the adf8/11 mutant,actinlaments are disordered at the adf8/11 root hair tips,and actin turn-over is attenuated.Secretory and recycling vesicles do not aggregate in the apical region of adf8/11 root hairs at high pH.Together,our results suggest that,under long-term exposure to high extracellular pH,ADF8/11 may establish and maintain the tip-focused F-actin meshwork to regulate polar trafcking of secretory/recycling vesicles at the root hair tips,thereby promoting root hair elongation.
基金Scientific Research Project of Hunan Provincial Health Commission,Grant/Award Number:C2023030765University-Hospital Joint-Fund of Hunan University of Chinese Medicine,Grant/Award Number:2022XYLH198+2 种基金Key Plans of Hunan Administration Traditional Chinese Medicine,Grant/Award Number:A2023039Technology Plan Project of Zhuzhou City,Hunan Province,Grant/Award Number:2021-009Fund for Creative Research Group of Affiliated First Hospital of Hunan Traditional Chinese Medical College,Grant/Award Number:2021 B-003。
文摘Background:The pathogenesis of multiple sclerosis(MS)and experimental autoimmune encephalomyelitis(EAE-an animal model of MS)is primarily mediated by T cells.However,recent studies have only focused on interleukin(IL)-17-secreting CD4^(+)T-helper cells,also known as Th17 cells.This study aimed to compare Th17 cells and IL-17-secreting CD8^(+)T-cytotoxic cells(Tc17)in the context of MS/EAE.Methods:Female C57BL/6 mice were immunized with myelin oligodendrocyte glycoprotein peptides 35-55(MOG35-55),pertussis toxin,and complete Freund's adjuvant to establish the EAE animal model.T cells were isolated from the spleen(12-14 days postimmunization).CD4^(+)and CD8^(+)T cells were purified using isolation kit and then differentiated into Th17 and Tc17,respectively,using MOG35-55 and IL-23.The secretion levels of interferon-(IFN-γ)and IL-17 were measured via enzyme-linked immunosorbent assay using cultured CD4^(+)and CD8^(+)T cell supernatants.The pathogenicity of Tc17 and Th17 cells was assessed through adoptive transfer(tEAE),with the clinical course assessed using an EAE score(0-5).Hematoxylin and eosin as well as Luxol fast blue staining were used to examine the spinal cord.Purified CD8^(+)CD3^(+)and CD4^(+)CD3^(+)cells differentiated into Tc17 and Th17 cells,respectively,were stimulated with MOG35-55 peptide for proliferation assays.Results:The results showed that Tc17 cells(15,951±19855VS.55,709±4196cpm;p<0.050)exhibited a weaker response to highest dose(20μg/mL)MOG35-55 than Th17 cells.However,this response was not dependent on Th17 cells.After the 48h stimulation,at the highest dose(20μg/mL)of MOG35-55.Tc17 cells secreted lower levels of IFN-(280.00±15.00vs.556.67±15.28pg/mL,p<0.050)and IL-17(102.67±5.86 pg/mL vs.288.33±12.58 pg/mL;p<0.050)than Th17 cells.Similar patterns were observed for IFN-γsecretion at 96 and 144h.Furthermore,Tc17 cell-induced tEAE mice exhibited similar EAE scores to Th17 cell-induced tEAE mice and also showed similar inflammation and demyelination.Conclusion:The degree of pathogenicity of Tc17 cells in EAE is lower than that of Th17 cells.Future investigation on different immune cells and EAE models is warranted to determine the mechanisms underlying MS.
文摘目的 探讨谷氨酰转肽酶(gamma-glutamyl transferase,GGT)<300 U/L临床疑诊为胆道闭锁患儿的临床特征。方法 收集2012年6月至2018年9月由广西医科大学第一附属医院儿科转至小儿外科收治的GGT<300 U/L临床疑诊为胆道闭锁的84例患儿的临床资料,根据术中胆道造影的诊断结果分为胆道闭锁组和胆汁淤积性肝炎组;其中确诊胆道闭锁33例(胆道闭锁组),39.29%(33/84),胆汁淤积性肝炎51例(胆汁淤积性肝炎组),60.71%(51/84)。分析两组患儿在肝功能、胆固醇、血乳酸、血氨、血常规和肝脏病理学等方面的特点和差异。结果 胆道闭锁组就诊日龄及手术日龄显著高于胆汁淤积性肝炎组,差异具有统计学意义(P<0.05)。胆道闭锁组GGT、总胆汁酸(total bile acid,TBA)值显著高于胆汁淤积性肝炎组,差异具有统计学意义(P<0.05)。GGT和TBA的曲线下面积(area under the curve,AUC)分别为0.715和0.72;GGT、TBA值两项指标联合分析,AUC为0.739,诊断价值均为中等,所得阈值灵敏性及特异性均不高。胆道闭锁组肝纤维化的比例显著高于胆汁淤积性肝炎组,其中21.21%(7/33)的胆道闭锁患儿存在肝硬化,所有胆汁淤积性肝炎组的患儿无肝硬化,胆道闭锁组肝纤维化程度与就诊日龄、GGT呈正相关(R=0.503,F=6.321,P=0.001)。胆道闭锁肝组织胆栓形成和胆管增生均显著高于胆汁淤积性肝炎(P<0.05)。结论 GGT<300 U/L的疑似胆道闭锁的患儿,建议早期进行肝组织病理学检查或剖腹探查提高诊断的准确率。