Hupo powder promotes autophagy of menstrual blood-derived stem cells from patients with endometriosis

Objective:To explore the effect of Hupo powder(HP)on autophagy in menstrual blood-derived stem cells(MenSCs)with endometriosis(EMT).Methods:EMT MenSCs(E-MenSCs)and healthy MenSCs(H-MenSCs)were isolated from the menstrual blood of patients with EMT and healthy female participants,respectively.We identified their stem cells’characteristics via adipogenic and osteogenic differentiation.Twelve male SpragueeDawley rats received 0.9% NaCl and HP-dispensing granules by gastric irrigation to prepare blank serum and medicated serum,respectively.We used serum concentrations of 5%,10%,and 20%,each at administered times of 12,24,and 48 h to select the best condition.These cells were divided into three groups:blank serum of the control group,blank serum of the model group,and medicated serum of the HP group.H-MenSCs were used in the control group,while E-MenSCs were used in the model and HP groups.We analyzed cell viability using a cell counting kit-8 assay,observed cell morphology,evaluated the amounts of auto-phagosomes and autolysosomes by transmission electron microscopy,and detected the protein expression of autophagy markers(LC3-II and Beclin1)by Western blot.Results:E-MenSCs and H-MenSCs became long fusiform with a diffuse radial pattern,forming lipid droplets and calcium nodules after adipogenic and osteogenic differentiation.We then used the best conditiond 20% serum and 48 hdfor the subsequent experiments.In contrast to the model group,the HP group exhibited lower cell viability(=0.007),larger amounts of autophagosomes and autolysosomes(P<0.001 and P=0.001,respectively),and higher expression of LC3-II and Beclin1(P=0.021 and P=0.019,respectively).Conclusion:Hupo powder can promote autophagy in E-MenSCs,which might be one of the mechanisms underlying its therapeutic effects.

Surface modulation of MoS_(2)/O-ZnIn_(2)S_(4) to boost photocatalytic H_(2)evolution

To realize the continuous production of hydrogen energy,the efficient photocatalysts are required in the heterogeneous reaction for water splitting.Herein,we reported a surface modulation strategy,via doping oxygen atoms to tune the surface state of ZnIn_(2)S_(4)nanosheets with cocatalyst MoS_(2)modification,to enhance water adsorption and surface catalytic reaction for boosting the photocatalytic activity.Consequently,MoS_(2)/O-ZnIn_(2)S_(4)photocatalysts showed a remarkably superior photocatalytic H_(2)production performance of 4.002 mmol g^(-1)h^(-1)and an apparent quantum yield(AQY)of~2.53%,5.4 folds higher than ZnIn_(2)S_(4).Using operando infrared spectroscopy and DFT calculation,we revealed the dynamic structural evolution,as well as the active sites for water adsorption and the catalytic reaction at the MoS_(2)/O ZnIn_(2)S_(4)interface.This work reveals the effect of surface modulation on the photocatalytic activity for MoS_(2)/O-ZnIn_(2)S_(4)and offers a feasible method to devise excellent nanomaterial photocatalysts for H_(2)production.

Assessment of ecological civilization construction from the perspective of environment and health in China

This study innovatively evaluated ecological civilization in China from the perspective of environment and health.A Composite Environmental Health Index(CEHI)was constructed based on the Driving force-Pressure-State-Impact-Response(DPSIR)and Coupling Coordination Degree(CCD)models.Results showed that significant and sustained improvements were observed in the ecological environment after ecological civilization,while economic development continued to progress at a steady pace.However,the advancement in population health(impact subsystem),exhibited comparatively modest progress,potentially linked to issues such as demographic aging and the enduring consequences of past exposure to environmental pollutants.At the provincial level,the regional development was uneven.The CEHI performance was highest in the eastern regions,followed by the central regions,with the western regions showing the least progress.Beijing,Guangdong,Jiangsu,Shanghai,and Zhejiang emerged as top performers with higher CEHI scores,which can be attributed to their favorable geographical positioning and the response subsystem.Conversely,northeastern regions(Heilongjiang,Jilin,and Liaoning)and northwestern regions(Shaanxi,Gansu,Ningxia,and Qinghai)experienced limited advancements in post-ecological civilization implementation.For these underperforming regions,there is a pressing need to intensify efforts aimed at enhancing their response subsystems.In summary,China's pursuit of ecological civilization has yielded significant successes,potentially offering valuable insights for other nations striving for sustainable development.The ecological civilization model's integration of ecological environmental protection into economic,political,cultural,and social constructs may serve as a meaningful reference for the sustainable development of other countries.