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Toll-like receptor 4-related regulation of Ornithogalum caudatum extract on inflammatory responses in LPS activated macrophages
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作者 Xin HAN Jian SONG +7 位作者 Jing ZHANG ya-mei li Ying FAN Dan-yang SHAO li-shuang HOU li-hua liAN Ji-xing NAN Yan-ling WU 《中国药理学与毒理学杂志》 CAS CSCD 北大核心 2018年第4期284-285,共2页
OBJECTIVE To investigate toll-like receptor 4(TLR4)-related the regulation of Ornithogalum caudatum extract(OCE) on inflammatory responses in lipopolysaccharide(LPS) activated macro.phages.METHODS Primary peritoneal m... OBJECTIVE To investigate toll-like receptor 4(TLR4)-related the regulation of Ornithogalum caudatum extract(OCE) on inflammatory responses in lipopolysaccharide(LPS) activated macro.phages.METHODS Primary peritoneal macrophage,Raw 264.7,and THP-1 were incubated in 96-well plate for 24 h and treated with OCE of the concentration of 0-400 μg/ml for 4 h.The viability of cells was measured by MTT assay.Specific concentrations of OCE were added into the medium of primary peri.toneal macrophage,Raw 264.7,and THP-1,respectively,then following with lipopolysaccharides(LPS).Cells were harvested and the total cellular protein and nuclear protein were extracted,and the protein content was determined using BCA protein assay Kit.The expressions of TLR4,inducible nitric oxide synthase(iNOS),cyclooxygenase 2(COX-2),α-inhibitor of NF-κB(IκB-α) and nuclear factor-κB(NF-κB) were assayed by Western blot.The expressions of interleukin-1α(IL-1α),interleukin-1β(IL-1β),interleukin-18(IL-18),and tumor necrosis factor-α(TNF-α) were measured by RT-PCR.RESULTS The results of MTT showed that OCE has no cytotoxicity in Raw 264.7 cells between 1.56 μg/ml and 400 μg/ml.Compared with normal group,the expressions of TLR4,iNOS,COX-2,NF-κB and IL-1α,IL-1β,IL-18,TNF-α,the level of nitric oxide(NO) were significantly increased by LPS stimulation,while OCE pretreat.ment reduced these increase induced by LPS.However,OCE pretreatment reversed the reduction of IκB-α after LPS stimulation.CONCLUSION OCE might suppress TLR4 expression and block the inflamma.tion process of NF-κB and iNOS,further decrease the expression of COX-2 and inhibit the release of inflammatory factors. 展开更多
关键词 尾砂提取物 巨噬细胞 炎症 临床分析 治疗方法
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Establishing a Quality Control System of Hedyotis corymbosa
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作者 Yan liN Hong-Quan li +6 位作者 Bo-Hou XIA Zhi-Min ZHANG ya-mei li Xiao-Qing ZHANG Ping WU li-Mei liN Duan-Fang liAO 《Digital Chinese Medicine》 2018年第4期302-309,共8页
Objective Hedyotis corymbosa(Shui Xian Cao,水线草,HC)is the main antitumor medicine in China and other southeast Asian countries.A comprehensive quality control system of HC was established in this study,as the curren... Objective Hedyotis corymbosa(Shui Xian Cao,水线草,HC)is the main antitumor medicine in China and other southeast Asian countries.A comprehensive quality control system of HC was established in this study,as the current system was ineffective.Methods Microscopy was used to observe the characteristics of the powder and cross-sectional tissue.Thin layer chromatography(TLC)was employed to separate HC from Hedyotis diffusa(Bai Hua She She Cao,白花蛇舌草,HD).Simultaneously,high-performance liquid chromatography(HPLC)was used to establish the characteristic spectrum of HC and determine the concentrations of hedyotiscone A.Regarding the issue of systematicness,concentrations of water,total ash,acid-insoluble ash,heavy metals(including lead,arsenic,mercury,cadmium and copper)and pesticide residue were measured by the methods described in the 2015 edition of Chinese Pharmacopoeia.Results Calcium oxalate needle crystal bundles and oil drops were observed in the cross-sectional tissue.Three characteristic blue fluorescent spots were observed in the HC samples by TLC.There were five distinct characteristic peaks in HC but not in HD,and hedyotiscone A was the sharpest characteristic peak.The generated linear equation of hedyotiscone A was Y=3.15×108X+312.04(r2=1.000)between 0.00005270 and 0.002108 mg/mL,and the average recovery was 100.42%.Precision[relative standard deviation(RSD)0.92%],repeatability(RSD 0.14%),and recovery(RSD 0.85%)met the requirements for quantitative analysis.Hedyotiscone A concentrations ranged from 0.00017%~0.00113%across different habitats.Concentrations of water,ash and acid-insoluble ash were 8.80%~11.2%,7.00%~15.3%and 0.440%~7.70%,respectively.Concentrations of Pb,As,Hg,Cd and Cu were 1.60~4.60,0.650~1.60,0.0300~0.100,0.240~0.690 and 10.1~16.6μg/g,respectively.Pesticide residues were less than 0.00100 ppm.Conclusion The quality control system can effectively distinguish HC from HD,so as to comprehensively control the quality of HC,to provide references for the clinical use of HC. 展开更多
关键词 Hedyotis corymbosa Hedyotis diffusa Hedyotiscone A TLC HPLC Quality standard
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Disaster effects of climate change in High Mountain Asia:State of art and scientific challenges Disaster effects of climate change in High Mountain Asia:State of art and scientific challenges 被引量:1
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作者 Hao WANG Bin-Bin WANG +13 位作者 Peng CUI Yao-Ming MA Yan WANG Jian-Sheng HAO Yu WANG ya-mei li li-Jun SUN Jiao WANG Guo-Tao ZHANG Wei-Mo li Yu LEI Wen-Qing ZHAO Jin-Bo TANG Chao-Yue li 《Advances in Climate Change Research》 SCIE CSCD 2024年第3期367-389,共23页
High Mountain Asia(HMA)shows a remarkable warming tendency and divergent trend of regional precipitation with enhanced meteorological extremes.The rapid thawing of the HMA cryosphere may alter the magnitude and freque... High Mountain Asia(HMA)shows a remarkable warming tendency and divergent trend of regional precipitation with enhanced meteorological extremes.The rapid thawing of the HMA cryosphere may alter the magnitude and frequency of nature hazards.We reviewed the influence of climate change on various types of nature hazards in HMA region,including their phenomena,mechanisms and impacts.It reveals that:1)the occurrences of extreme rainfall,heavy snowfall,and drifting snow hazards are escalating;accelerated ice and snow melting have advanced the onset and increased the magnitude of snowmelt floods;2)due to elevating trigger factors,such as glacier debuttressing and the rapid shift of thermal and hydrological regime of bedrock/snow/ice interface or subsurface,the mass flow hazards including bedrock landslide,snow avalanche,ice-rock avalanches or glacier detachment,and debris flow will become more severe;3)increased active-layer detachment and retrogressive thaw slumps slope failures,thaw settlement and thermokarst lake will damage many important engineering structures and infrastructure in permafrost region;4)multi-hazards cascading hazard in HMA,such as the glacial lake outburst flood(GLOF)and avalanche-induced mass flow may greatly enlarge the destructive power of the primary hazard by amplifying its volume,mobility,and impact force;and 5)enhanced slope instability and sediment supply in the highland areas could impose remote catastrophic impacts upon lowland regions,and threat hydropower security and future water shortage.In future,ongoing thawing of HMA will profoundly weaken the multiple-phase material of bedrock,ice,water,and soil,and enhance activities of nature hazards.Compounding and cascading hazards of high magnitude will prevail in HMA.As the glacier runoff overpasses the peak water,low flow or droughts in lowland areas downstream of glacierized mountain regions will became more frequent and severe.Addressing escalating hazards in the HMA region requires tackling scientific challenges,including understanding multiscale evolution and formation mechanism of HMA hazard-prone systems,coupling thermo‒hydro‒mechanical processes in multi-phase flows,predicting catastrophes arising from extreme weather and climate events,and comprehending how highland hazards propagate to lowlands due to climate change. 展开更多
关键词 High Mountain Asia Climate change Cryosphere degradation Nature hazards Disaster risk
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New Diterpenoids from Clerodendranthus spicatus
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作者 ya-mei li Bing Xiang +2 位作者 Xiao-Zheng li Yong-Ming Yan Yong-Xian Cheng 《Natural Products and Bioprospecting》 CAS 2017年第3期263-267,共5页
Two new diterpenoids,neoorthosiphonones B and C(1 and 2),and one known diterpenoid,were isolated from the aerial parts of Clerodendranthus spicatus.Their structures including absolute configurations were determined by... Two new diterpenoids,neoorthosiphonones B and C(1 and 2),and one known diterpenoid,were isolated from the aerial parts of Clerodendranthus spicatus.Their structures including absolute configurations were determined by comprehensive spectroscopic analyses and X-ray crystallographic methods.No compound was found to inhibit fibronectin production at the concentration of 20μM. 展开更多
关键词 Clerodendranthus spicatus DITERPENOID Neoorthosiphonones B and C
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Combined Effects of Electroacupuncture and Behavioral Training on Learning-Memory Ability and Event-Related Potential P300 in Rats with Mid/Advanced Cerebral Infarction 被引量:13
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作者 Qian Yu Xiao-Hong li +5 位作者 Wei Jiang ya-mei li Xiao-Dong Duan Wei Wei Jing Fu lin Huang 《Chinese Medical Journal》 SCIE CAS CSCD 2018年第18期2172-2178,共7页
Background: The effectiveness of the combination of electroacupuncture (EA) and behavioral training (BT) for mid/advanced cerebral infarction (M/ACI) and related mechanisms remains unclear. This study aimed to ... Background: The effectiveness of the combination of electroacupuncture (EA) and behavioral training (BT) for mid/advanced cerebral infarction (M/ACI) and related mechanisms remains unclear. This study aimed to investigate the combined effects on the learning-memory ability and event-related potential P300 in rats with M/ACI. Methods: Eighty rats with M/ACI were divided into Group Model (M), Group EA, Group BT, and Group EA-BT (n = 20) according to the random number with five healthy rats in Group Control (CON). On the 6th week after modeling, EA, BT, and EA-BT were given to Group EA, Group BT, and Group EA-BT, respectively, whereas Group M and Group CON were not given any intervention. Y-maze test and P300 were recorded before and after the intervention. Results: After intervention, the P300 latency was lower and the amplitude was higher in the Group EA-BT, Group EA, and Group BT than before (for latency, t = -7.638, -4.334, and -5.916; for amplitude, t = 8.125, 3.846, and 5.238; P 〈 0.01 ), with Group EA-BT superior to Group EA (for latency, t = -3.708; for amplitude, t = 3.653; P 〈 0.01) and Group BT (for latency, t = -2.067; for amplitude, t = 2.816; P 〈 0.05), with no significant difference between Group BT and EA (for latency, t = -1.439; for amplitude, t = 1.075; P 〉 0.05). While the performances of Y-maze tests in the Group EA-BT, Group EA, and Group BT were all better than before (t = 10.359, 4.520, and 7.791, P 〈 0.01), with Group EA-BT better than Group EA (t = 5.627, P 〈 0.01 ) and Group BT (t = 2.913, P 〈 0.01 ) respectively, and Group BT better than Group EA (t = 2.912, P 〈 0.01). Conclusion: EA or BT can affect P300 in rats with M/ACI, and the combination of these two methods can significantly improve the learning-memory ability. 展开更多
关键词 Cerebral Infarction ELECTROACUPUNCTURE Event-Related Potentials Exercise Therapy P300 RATS
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Application and prospect of toxicity quality markers of Chinese materia medica based on metabolomics 被引量:6
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作者 Peng-jie Zhang ya-mei li +4 位作者 Ya-ni Zhang Wei Huang Yu-bo li Yan-jun Zhang Chang-xiao liu 《Chinese Herbal Medicines》 CAS 2018年第2期108-116,共9页
In view of the problem that the quality evaluation indicators divorced from effectiveness and safety in Chinese materia medica(CMM), Prof. Chang-xiao Liu et al have put forward a concept of quality markers(Q-marker... In view of the problem that the quality evaluation indicators divorced from effectiveness and safety in Chinese materia medica(CMM), Prof. Chang-xiao Liu et al have put forward a concept of quality markers(Q-markers) of CMM, which provided a new thought for improving the CMM quality control method. The toxicity Q-markers of CMM are described as follows, the early toxicity prediction of CMM, the discovery, formation and susceptibility research of toxicity Q-markers of CMM. Metabolomics is a systematic biology approach, closely related to the toxicity phenotype of organism, has unique advantages in toxicity Q-markers research. The toxicity Q-markers of CMM are explained from the following four aspects in this review: 1) the establishment of toxicity prediction model of CMM, which is based on metabolomics,pattern recognition, and other techniques; 2) the discovery of toxicity Q-markers of CMM based on metabolomic, which is meaning that the toxicity Q-markers are interpreted regarding to the chemical composition analysis of CMM, preliminary screening and verification of the toxicity Q-markers and toxicity mechanism research; 3) the research on the formation of toxicity Q-markers based on metabolomics,which is described from biosynthetic pathway analysis and the secondary metabolites analysis of toxicity Q-markers; and 4) the susceptibility study of toxicity Q-markers based on metabolomics, namely, the key targets, and metabolic pathways of toxicity Q-markers are explained according to the target organ toxicity susceptibility caused by CMM, and then the toxicity susceptibility mechanism of CMM is clarified. Therefore, the application of metabolomics helps to systematically and comprehensively discover the quality control indicators related to the CMM safety, which further provides a rational evidence for the clinical application of toxicity of CMM. 展开更多
关键词 Chinese materia medica metaholomics toxicity Q-markers
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Influence of renal function by transplant operation for living-related kidney donors in a short term
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作者 Chong-Wei Zhang Guo Huang +3 位作者 Qing-Wei Wang Xing-Chao liu ya-mei li li Wang 《Chinese Medical Journal》 SCIE CAS CSCD 2019年第5期618-621,共4页
To the Editor:Data from the Global Observatory on Donation and Transplantation (GODT) indicate that of the 126,670 solid organs reported to be transplanted in 2015, kidneys made up 66% of these organs.
关键词 RENAL function TRANSPLANT operation living-related KIDNEY DONORS
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