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A NOVEL POLYMER ENZYME LINKED IMMUNOBLOTTING ASSAY METHOD AND ITS APPLICATIONS(Ⅱ) 被引量:1
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作者 Feng Hua LIU Feng LIU +2 位作者 yan zhuo deng Yun E ZENG Ren Xi zhuo (Dept. of Chem., Dept. of Anal. and Testing Scl.,Wuhan Univ., Wuhan,430072) 《Chinese Chemical Letters》 SCIE CAS CSCD 1994年第5期387-388,共2页
By using the characteristic that poly (N-isopropylacrylamide) can be adhered to callulose acetais-nitrate membrane in high retentior tightly and ramdly,we developed a novel immunoamay method- polymer enayme Hnked immu... By using the characteristic that poly (N-isopropylacrylamide) can be adhered to callulose acetais-nitrate membrane in high retentior tightly and ramdly,we developed a novel immunoamay method- polymer enayme Hnked immunoblotting amay method.Human serum HBsAg can detected as little as 10ng/ml with this immunoassay system. 展开更多
关键词 ITS METHOD APPLICATIONS AND
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INDIRECT THERMO-OPTICAL DETECTION FOR CAPILLARY ELECTROPHORESIS SEPARATION OF AMINO ACIDS
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作者 Ji Cun REN Bing Cheng LI +1 位作者 yan zhuo deng Jie Ke CHENG(Department of Chemistry. Department of Analysis and Testing Science Wuhan University. Wuhan. 130072) 《Chinese Chemical Letters》 SCIE CAS CSCD 1994年第9期767-768,共2页
In this letter, we first reported a novel method-indirect thermo-optical detection used in CE separation of native amino acids. By using methylene blue as background aborber,the detecion limit for lysine was 5×10... In this letter, we first reported a novel method-indirect thermo-optical detection used in CE separation of native amino acids. By using methylene blue as background aborber,the detecion limit for lysine was 5×10(-5)mol/L. 展开更多
关键词 THERMO AMINO CAPILLARY DETECTION FOR INDIRECT OF OPTICAL SEPARATION ACIDS
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A NOVEL POLYMER ENZYME LINKED IMMUNOASSAY METHOD AND ITS APPLICATIONS
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作者 Feng LIU Feng Hua LIU +2 位作者 Ren Xi zhuo yan zhuo deng Yun E ZHEN (Department of Chemistry, Wuhan Univ., Wuhan 430072) 《Chinese Chemical Letters》 SCIE CAS CSCD 1994年第4期325-326,共2页
During the course of study, we found that both poly (N-isopropylacrylamide ) (PNIP) and PNIP-Ab (enzyme-labelled antibody)could be adhere tightly to a cellulose acetale-nitrate membrane, and that the retention of PNI... During the course of study, we found that both poly (N-isopropylacrylamide ) (PNIP) and PNIP-Ab (enzyme-labelled antibody)could be adhere tightly to a cellulose acetale-nitrate membrane, and that the retention of PNIP-Ab on the membrane increased over 30-fold when compared with the unconjugated Ab.Thus we used this characteristic to develop a novel immunoassay method-polymer enzyme linked immunoassay method: homogeneous antigen-antibody reaction and heterogeneous separation process. When applied for detection of human serum HBsAg, this immunoassay system can detect as little as 1 ng/ml of human serum HBsAg. 展开更多
关键词 ITS A NOVEL POLYMER ENZYME LINKED IMMUNOASSAY METHOD AND ITS APPLICATIONS
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Application of Simultaneous Technique of Capillary Zone Electrophoresis Photothermal Interference Spectrometry for Monitoring Chelating Reaction of Light Rare Earth with Tribromoarsenazo
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作者 Yong Gang HU yan zhuo deng Jie Ke CHENG(Department of Chenustry, Center of Analysis and Testing, Wuhan Umversity, Wuhan 430072) 《Chinese Chemical Letters》 SCIE CAS CSCD 1997年第5期425-426,共2页
A novel high sensihtity, small-volume photothermal intheence detector has beenintroduced for capillap zone electrophoresis separation analysis. The utility of thes sdriulboconstecheque for momtomp chelating reachon of... A novel high sensihtity, small-volume photothermal intheence detector has beenintroduced for capillap zone electrophoresis separation analysis. The utility of thes sdriulboconstecheque for momtomp chelating reachon of light rare earth with tribromoarsenazo has beenreported. 展开更多
关键词 Application of Simultaneous Technique of Capillary Zone Electrophoresis Photothermal Interference Spectrometry for Monitoring Chelating Reaction of Light Rare Earth with Tribromoarsenazo
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