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Positive Selection Analysis of VP1 Genes of Worldwide Human Enterovirus 71 Viruses 被引量:8
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作者 Wei-feng SHI Zhong zhang +4 位作者 Ai-she DUN yan-zhou zhang Guang-fu YU Dong-ming ZHUANG Chao-dong ZHU 《Virologica Sinica》 SCIE CAS CSCD 2009年第1期59-64,共6页
Human enterovirus 71 viruses have been long circulating throughout the world. In this study, we performed a positive selection analysis of the VP1 genes of capsid proteins from Enterovirus 71 viruses. Our results show... Human enterovirus 71 viruses have been long circulating throughout the world. In this study, we performed a positive selection analysis of the VP1 genes of capsid proteins from Enterovirus 71 viruses. Our results showed that although most sites were under negative or neutral evolution, four positions of the VP1 genes were under positive selection pressure. This might account for the spread and frequent outbreaks of the viruses and the enhanced neurovirulence. In particular, position 98 might be involved in neutralizing antibodies, modulating the virus-receptor interaction and enhancing the virulence of the viruses. Moreover, both positions 145 and 241 might correlate to determine the receptor specificity. However, these positions did not display much difference in amino acid polymorphism. In addition, no position in the VP1 genes of viruses isolated from China was under positive selection. 展开更多
关键词 Human enterovirus 71 Positive selection pressure VP1
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Selection Pressure on Haemagglutinin Genes of H9N2 Influenza Viruses from Different Hosts 被引量:1
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作者 Wei-feng SHI Ai-she DUN +4 位作者 Zhong zhang yan-zhou zhang Guang-fu YU Dong-ming ZHUANG Chao-dong ZHU 《Virologica Sinica》 SCIE CAS CSCD 2009年第1期65-70,共6页
Positive selection and differential selective pressure analyses were carried out to study Haemagglutinin (HA) genes of H9N2 influenza viruses from different hosts in this paper. Results showed that, although most posi... Positive selection and differential selective pressure analyses were carried out to study Haemagglutinin (HA) genes of H9N2 influenza viruses from different hosts in this paper. Results showed that, although most positions in HAs were under neutral or purifying evolution, a few positions located in the antigenic regions and receptor binding sites were subject to positive selection and some of them were even positively selected at the population level. In addition, there were always some positions differentially selected for viruses from different hosts. Both selection pressure working on HA codons and positions differentially selected might account for the extension of the host range and adaptations to different hosts of H9N2 influenza viruses. 展开更多
关键词 H9N2 Avian influenza virus HAEMAGGLUTININ Selection pressure
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Proteotyping:A New Approach Studying Influenza Virus Evolution at the Protein Level 被引量:1
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作者 Wei-feng SHI Zhong zhang +3 位作者 Lei PENG yan-zhou zhang Bin LIU Chao-dong ZHU 《中国病毒学》 CSCD 2007年第5期405-411,共7页
Phylogenetic methods have been widely used to detect the evolution of influenza viruses.However,previous phylogenetic studies of influenza viruses do not make full use of the genetic information at the protein level a... Phylogenetic methods have been widely used to detect the evolution of influenza viruses.However,previous phylogenetic studies of influenza viruses do not make full use of the genetic information at the protein level and therefore cannot distinguish the subtle differences among viral genes.Proteotyping is a new approach to study influenza virus evolution.It aimed at mining the potential genetic information of the viral gene at the protein level by visualizing unique amino acid signatures(proteotypes).Neuraminidase gene fragments of some H5N1 avian influenza viruses were used as an example to illustrate how the proteotyping method worked.Bayesian analysis confirmed that the NA gene tree was mainly divided into three lineages.The NA proteotype analysis further suggested there might be multiple proteotypes within these three lineages and even within single genotypes.At the same time,some proteotypes might even involve more than one genotype.In particular,it also discovered some amino acids of viruses of some genotypes might co-reassort.All these results proved this approach could provide additional information in contrast to results from standard phylogenetic tree analysis. 展开更多
关键词 鸟类流行感冒病毒 蛋白质 基因型 H5N1 神经氨酸酶 蛋白分型技术
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Application of DNA barcoding to the identification of Hymenoptera parasitoids from the soybean aphid (Aphis glycines) in China 被引量:2
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作者 Qing-Song Zhou Yu-Qiang Xi +7 位作者 Fang Yu Xu zhang Xue-Jun Li Chun-Lai Liu Ze-Qing Niu Chao-Dong Zhu Ge-Xia Qiao yan-zhou zhang 《Insect Science》 SCIE CAS CSCD 2014年第3期363-373,共11页
Aphis glycines Matsumura is an important pest of soybean in Asia and North America. Hymenoptera parasitoids play a key role in the control of the soybean aphid. Thecorrect identification of parasitoids is a critical s... Aphis glycines Matsumura is an important pest of soybean in Asia and North America. Hymenoptera parasitoids play a key role in the control of the soybean aphid. Thecorrect identification of parasitoids is a critical step that precedes the assessment of their potential biological control agents. Accurate identification of the majority of the speciesattacking the soybean aphid often requires elaborate specimen preparation and expert taxonomic knowledge. In this study, we facilitated the identification of soybean aphidparasitoids by applying a DNA barcoding approach following a preliminary morphological identification. We generated DNA sequence data from the mitochondrial COI gene andthe D2 region of 28S rDNA to assess the genetic variation within and between parasitoid species emerging from the soybean aphid in China. Fifteen Hymenoptera parasitoid speciesbelonging to 10 genera of five families were identified with little intra-specific variation (0.09% ± 0.06% for 28S and 0.36% ± 0. 18% for COI) and large inter-specific divergence(30.46% ± 3.42% for 28S and 20.4% ± 1.20% for COI). 展开更多
关键词 biological control COL 28S-D2 molecular identification species delimitation
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A new surgical technique for post-myocardial infarction ventricular septal rupture with hemodynamic instability 被引量:2
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作者 Chao Liu Li-Li Xiao +7 位作者 Lu Wang Fu-Qiang Sun Xing-Xing Yao yan-zhou zhang Xiao-Yan Zhao Ling Li Xiao-Fang Wang Jian-Zeng Dong 《Chinese Medical Journal》 SCIE CAS CSCD 2021年第8期981-983,共3页
Ventricular septal rupture(VSR)is a rare but lethal complication of acute myocardial infarction(AMI).[1]Emergent repair surgery provides the only chance for survival for patients with VSR;however,the perioperative mor... Ventricular septal rupture(VSR)is a rare but lethal complication of acute myocardial infarction(AMI).[1]Emergent repair surgery provides the only chance for survival for patients with VSR;however,the perioperative mortality rate remains extremely high at the early phase.[2] Elective surgery is widely utilized in real-world clinical practice and is associated with improved prognosis.[3] Nevertheless,the results of elective surgery might be a manifestation of survival bias,as it is usually performed in relatively stable patients with VSR who are expected to survive the early stage and have a better prognosis than patients complicated with hemodynamic instability in the early stage.[4]In this study,we aimed to present a novel surgical repair technique that can be safely,feasibly,and effectively used in hemodynamically unstable patients with VSR in the acute phase and to report the preliminary results. 展开更多
关键词 RUPTURE ACUTE SURGICAL
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Isolation of microsatellite loci in the pollinating fig wasp of Ficus hispida, Ceratosolen solmsi 被引量:1
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作者 Hao Yu Tong-Xin zhang +4 位作者 Hao-Yuan Hu Li-Ming Niu Hui Xiao yan-zhou zhang Da-Wei Huang 《Insect Science》 SCIE CAS CSCD 2008年第4期303-306,共4页
Microsatellite loci were isolated for Ceratosolen solmsi, pollinator of the dioecious Ficus hispida. We developed nine polymorphic microsatellite loci based on the method of polymerase chain reaction isolation of micr... Microsatellite loci were isolated for Ceratosolen solmsi, pollinator of the dioecious Ficus hispida. We developed nine polymorphic microsatellite loci based on the method of polymerase chain reaction isolation of microsatellite arrays (PIMA). Enrichment of genomic libraries was performed by random amplified polymorphic DNA (RAPD). A subset of 38 positive clones was sequenced; 15 clones showed microsatellite loci. We tested 15 designed primer pairs and nine of them produced polymorphic amplification in 48 individual wasps collected from different fruits of the dioecious host fig Ficus hispida in China. Among the 48 individuals, 49 alleles were obtained at the nine loci. The observed heterozygosity ranged between 0.357 and 0.634. 展开更多
关键词 FICUS fig wasp MARKERS microsatellite loci POLYMORPHISM PRIMERS
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