Li-promoted C_(3)N_(4) catalyst for efficient isomerization of glucose into fructose at 50℃ in water

Efficient and selective glucose-to-fructose isomerization is a crucial step for production of oxygenated chemicals derived from sugars,which is usually catalyzed by base or Lewis acid heterogeneous catalyst.However,high yield and selectivity of fructose cannot be simultaneously obtained under mild conditions which hamper the scale of application compared with enzymatic catalysis.Herein,a Li-promoted C_(3)N_(4) catalyst was exploited which afforded an excellent fructose yield(40.3 wt%)and selectivity(99.5%)from glucose in water at 50℃,attributed to the formation of stable Li–N bond to strengthen the basic sites of catalysts.Furthermore,the so-formed N_(6)–Li–H_(2)O active site on Li–C_(3)N_(4) catalyst in aqueous phase changes the local electronic structure and strengthens the deprotonation process during glucose isomerization into fructose.The superior catalytic performance which is comparable to biological pathway suggests promising applications of lithium containing heterogeneous catalyst in biomass refinery.

Longitudinal Studies on Illness Perceptions in Cancer Patients: A Scoping Review

Objective:To conduct a scoping review of longitudinal studies related to disease perception in cancer patients and to provide a reference for the improvement of disease perception in cancer patients.Methods:Using the scoping method,PubMed,Web of Science,The Cochrane Library,Medline,CNKI,Wanfang Database,VIP Chinese Scientific and Technical Journals Database,and China Biomedical Literature Database were searched up to July 2023 for analysis and discussion of the included literature.Results:A total of 19 articles were included,distributed across 9 countries including China,the Netherlands,and the United Kingdom,with 8 articles published in China.The study subjects included patients with breast cancer,colorectal cancer,laryngeal cancer,lung cancer,esophageal cancer,and bladder cancer.The content covered dynamic changes in disease perception,the impact of disease perception,and the influencing factors of disease perception.Conclusion:The number of longitudinal studies on disease perception in cancer patients is gradually increasing and becoming more diverse.However,issues such as a small total number of studies,single research methods,and short observation periods persist.In the future,it is necessary to conduct in-depth longitudinal research on disease perception in cancer patients,expand the scope of research fields,and provide more comprehensive theories to guide clinical treatment and nursing practices,ultimately improving the treatment outcomes and quality of life for cancer patients.

基于SEER数据库构建原发性纵隔生殖细胞瘤患者生存预测的列线图

背景与目的原发性纵隔生殖细胞瘤(primary mediastinal germ cell tumor,PMGCT)是一种相对罕见且偶尔会具有高度侵袭性的纵隔肿瘤。目前对PMGCT的疾病特异性生存期(disease special survival,DSS)的相关研究报道较少,大数据分析亦相对较少,DSS预后模型也较为少见。本研究旨在探讨影响PMGCT DSS的预后相关因素,并构建简便、有效、可对PMGCT患者DSS预后情况进行预测的列线图。方法回顾性分析从监测、流行病学和最终结果(Surveillance,Epidemiology,and End Results,SEER)数据库提取的1975年-2019年共347例PMGCT患者的临床病理资料。采用Kaplan-Meier法及Log-rank检验估计DSS。执行Cox比例风险回归模型筛选影响预后的独立危险因素,构建个体化列线图预测PMGCT患者的3年、5年、8年DSS。通过受试者工作特征(receiver operating characteristic,ROC)曲线、校正曲线及决策曲线分析(decision curve analysis,DCA)评估模型的预测精度。结果PMGCT患者的3年、5年、8年生存率分别为84.6%、83.6%、83.3%。单因素Cox回归分析显示组织学分型、手术与否、年龄、肿瘤大小、肿瘤转移情况及肿瘤分期6项因素可影响PMGCT的预后(P<0.05),多因素Cox回归分析显示组织学分型、手术与否、年龄、肿瘤大小是PMGCT患者预后的独立危险因素(P<0.05),利用这些独立危险因素构建了列线图模型。ROC的曲线下面积(area under the curve,AUC)为0.824,3年、5年、8年生存时间的校正曲线以及DCA曲线,三者结果提示本研究的列线图评估预测结果与真实结果之间有良好的一致性。结论PMGCT中组织学分型为精原细胞瘤的患者比非精原细胞瘤患者预后更佳,年龄>40岁、肿瘤大小≥15 cm且未进行过手术治疗的患者预后不佳。列线图模型可以对PMGCT患者的DSS进行准确直观的预测。

Physiological mechanisms underlying reduced photosynthesis in wheat leaves grown in the field under conditions of nitrogen and water deficiency

Reduced photosynthesis results directly from nitrogen or water deficiency in wheat plants,and leads to a decrease in grain yield.In this study,by measuring the effects of water and N deficiencies,both individually and combined,we characterized the responses of wheat(Triticum aestivum L.Yumai 49-198)plants to these two deficiencies using physiological measurements and comparative proteomics.Significant decreases in grain yield and leaf photosynthetic performance were observed in all deficiency conditions,and 106 photosynthetic proteins that showed responses were identified.Nitrogen deficiency induced the least change in photosynthetic proteins,and similar changes in most of these proteins were also observed for the combined nitrogen and water deficiencies.Water deficiency induced the largest change in photosynthetic proteins and resulted in the lowest 1000-kernel weight.Severe decreases in photosynthesis in both the water-deficiency and combined N and water deficiency groups were reflected mainly in an imbalanced ATP/NADPH ratio associated with the light reaction,which influences carbon metabolism in the Calvin cycle.Photorespiration was respectively stimulated or inhibited by N or water deficiency,while suppression of photorespiratory flux and activation of nitrogen recycling were observed in the combined N and water deficiency treatments.Comparison of photosynthetic proteins between experimental sites suggested that precipitation affected linear electron flow in the photoreaction,and thus photosynthetic efficiency.Our results provide a baseline for future studies of the roles of these photosynthetic proteins in the response to N or water deficiency and their effect on 1000-kernel weight.

Clinical Application of Quantitative Nursing for Lower Cranial Nerves Injury after Cerebellopontine Angle Tumors

Objective: To retrospectively analyze the clinical utility of quantitative nursing measures of 10 cases of lower cranial nerves injury after cerebellopontine angle tumors surgery to provide the experience for improving the recovery rate and living quality of these patients. Methods: The clinical data of 10 cases of lower cranial nerves injury after cerebellopontine angle tumors surgery was analyzed. For problems such as dysphagia and dyspnea of these patients, the nursing care focused on strict monitoring, timely oxygen inhalation nursing, posture nursing, ventilator nursing, swallowing function training, etc. Results: After received quantitative care, 10 patients with lower cranial nerves injury after cerebellopontine angle tumors surgery were recovered well, and their symptoms such as dysphagia and dyspnea were gradually improved and safely discharged. Conclusion: Lower cranial nerves injury is one of the serious complications after removal of cerebellopontine angle tumors, which impacts the life and health of patients. Caregivers should accurately understand and analyze the symptoms, and quantitative and targeted nursing measures for posterior cranial nerves injury are helpful in the postoperative rehabilitation of patients and improve their living quality.

Identification of a potential tumor suppressor gene, UBL3, in non-small cell lung cancer

Objective: Oncogenes have been shown to be drivers of non-small cell lung cancer(NSCLC), yet the tumor suppressing genes involved in lung carcinogenesis remain to be systematically investigated. This study aimed to identify tumor suppressing ubiquitin pathway genes(UPGs) that were critical to lung tumorigenesis.Methods: The 696 UPGs were silenced by an siRNA screening in NSCLC cells;the potential tumor suppressing UPGs were analyzed, and their clinical significance was investigated.Results: We reported that silencing of 11 UPGs resulted in enhanced proliferation of NSCLC cells, and four UPGs(UBL3, TRIM22, UBE2 G2, and MARCH1) were significantly downregulated in tumor samples compared to that in normal lung tissues and their expression levels were positively associated with overall survival(OS) of NSCLC patients. Among these genes, UBL3 was the most significant one. UBL3 expression was decreased in tumor samples compared to that in paired normal lung tissues in 59/86(68.6%) NSCLCs, was correlated with TNM stage and sex of NSCLC patients, and was significantly higher in non-smoking patients than in smoking patients. Silencing UBL3 accelerated cell proliferation and ectopic expression of UBL3 suppressed NSCLC in vitro and in vivo.Conclusions: These results showed that UBL3 represented a tumor suppressor in NSCLC and may have potential for use in therapeutics and for the prediction of clinical outcome of patients.

Generation of monoclonal antibodies against n-3 fatty acid desaturase

Dear Editor： Omega-3 polyunsaturated fatty acids （n-3 PUFAs） are essential fatty acids for normal cellular functions and have been used for prevention and treatment of many diseases, including coronary heart disease, dia- betes, and cancers. n-3 PUFAs and n-6 PUFAs have been shown to decrease and increase the severity of several human diseases, respectively. Unfortunately,

Aromatic bromination with hydrogen production on organic‐inorganic hybrid perovskite‐based photocatalysts under visible light irradiation

Aromatic bromides are important chemicals in nature and chemical industries.However,their tra‐ditional synthesis routes suffer from low atomic economy and pollutant formation.Herein,we show that organic-inorganic hybrid perovskite methylammonium lead bromide(MAPbBr_(3))nanocrystals stabilized in aqueous HBr solution can achieve simultaneous aromatic bromination and hydrogen evolution using HBr as the bromine source under visible light irradiation.By hybridizing MAPbBr_(3) with Pt/Ta_(2)O_(5) and poly(3,4‐ethylenedioxythiophene)polystyrene sulfonate as electron‐and hole‐transporting motifs,aromatic bromides were achieved from aromatic compounds with high yield(up to 99%)and selectivity(up to 99%)with the addition of N,N‐dimethylformamide or its analogs.The mechanistic studies revealed that the bromination proceeds via an electrophilic attack pathway and that HOBr may be the key intermediate in the bromination reaction.

Construction and Biological Function Analysis of a dldh Deletion Mutant Strain of Vibrio alginolyticus

[ Objeetlve] This study aimed to investigate the role of dihydrolipoamide dehydrogenase （DLDH） in the pathopoiesis of Vibrio alginolyticua. [ Method] Using suicide plasmid pRE112 as the vector, d/dh deletion mutant strain ZJO3Adldh was constructed with insertion inactivation mutation method by Overlap PCR and homologous recombination technology to compare and analyze the differences in the growth, swimming ability, enzyme activity, biofilm formation and pathogenicity between wild-type strain ZJ03 and deletion mutant strain ZJO3△dldh. [ Result] dldh deletion mutant strain ZJ03 △d/dh exhibited prolonged lag phase, significantly reduced swimming ability （P 〈 0.05 ）, significantly reduced enzyme activity （P 〈 0.05 ）, significantly reduced biofilm formation ability （P 〈 0.05 ） and remarkably reduced pathogenicity to Epinephelus coioides （P 〈0.05） compared to wild-type strain ZJ03. [ Conclusion] This study provided theoretical basis for revealing the pathogenic mechanisms of Vibrio from the perspective of bacterial energy metabolism.

Molecular Cloning and Bioinformatics Analysis of kdpD Gene from Vibrio alginolyticus

In this study, histidine kinase gene kdpD in the two-component regulatory system of Vibrio alginolyticus strain HY9901 was cloned for bioinformatics analysis. Sequence analysis revealed that kdpD gene （GenBank accession number： KJ544668） was 1 374 bp in length, encoding a putative protein of 457 amino acids. The predicted molecular weight （MW） of KdpE was 51.60 kD with a theoretical isoelectric point （pl） of 6.02. Using SignalP 4.0, TMHMM Server 2.0 and SoftBerry-Psite software, it was predicted that KdpE protein was equally located in Golgi apparatus, plasma membrane and endoplasmie reticulum （33.3%） , which did not contain a signal peptide but contained three transmembrane domains. KdpE protein had five casein kinase 1I phosphorylation sites, five protein kinase C phosphorylation sites and one cAMP- and cGMP-dependent protein kinase phosphorylation site. A phylogenetic tree was constructed by MEGA 5.0 software, which revealed that KdpD from V. alginolyticus had close genetic relationship with corresponding proteins from V. campbellii and V. parahaemolyticus. Using SWISS- MODEL Workspace, the three-dimensional structure of HATPase_c conserved domain in KdpD protein was constructed. These results may provide the basis for fur- ther studies on two-comoonent regulatory system KdpD/KdpE of V. alginolyticus.

Design and verification of an FPGA programmable logic element based on Sense-Switch pFLASH

This paper proposes a kind of programmable logic element(PLE)based on Sense-Switch pFLASH technology.By programming Sense-Switch pFLASH,all three-bit look-up table(LUT3)functions,partial four-bit look-up table(LUT4)functions,latch functions,and d flip flop(DFF)with enable and reset functions can be realized.Because PLE uses a choice of operational logic(COOL)approach for the operation of logic functions,it allows any logic circuit to be implemented at any ratio of combinatorial logic to register.This intrinsic property makes it close to the basic application specific integrated circuit(ASIC)cell in terms of fine granularity,thus allowing ASIC-like cell-based mappers to apply all their optimization potential.By measuring Sense-Switch pFLASH and PLE circuits,the results show that the“on”state driving current of the Sense-Switch pFLASH is about 245.52μA,and that the“off”state leakage current is about 0.1 pA.The programmable function of PLE works normally.The delay of the typical combinatorial logic operation AND3 is 0.69 ns,and the delay of the sequential logic operation DFF is 0.65 ns,both of which meet the requirements of the design technical index.

Structural Stability of Natural Magnesiochromite at High-Temperature-Pressure Conditions

The podiform chromitites in the Luobusha ophiolite have been thought to experience a very deep formation,but the maximum depth is still an open issue.Here,we have investigated the structural stability of natural magnesiochromite using the synchrotron-based powder X-ray diffraction and diamond anvil cells up to 48.6 GPa and 2450 K.The results have shown that spinel-type magnesiochromite first decomposes into corundum-type‘Cr_(2)O_(3)’+B1-type‘MgO’at 11–14 GPa and 1250–1450 K,then modified ludwigite(mLd)-type‘Mg_(2)Cr_(2)O_(5)’+corundum-type‘Cr_(2)O_(3)’at 14.3–20.5 GPa and 1300–2000 K,and finally CaTi_(2)O_(4)-type phase at 24.5 GPa.During the quenching procession from high-temperature-pressure conditions,the mLd-type phase appeared again and was kept at ambient conditions.We also obtained the isothermal equation states of spinel-type and CaTi_(2)O_(4)-type phases,revealing the composition effect on their elasticities.Based on the updated results,we propose chromitites could not experience pressure exceeding∼14.3 GPa(approximate maximum depth∼400 km)in the subduction-recycling genesis model.

Efficient epoxidation of propylene over non-noble nickel-based catalyst promoted by alkali metals

The application of non-noble metal catalysts in the catalytic direct gas-phase epoxidation of propylene with H2and O2to produce propylene oxide is valuable and challenging. The introduction of alkali metal promoters is one of the effective methods to improve the catalytic activity of catalysts. Herein, a series of alkali metal(Li, Na, K, Rb, and Cs)-promoted Ni/TS-1 catalysts were prepared to deeply understand the effect of alkali metals on the structure-activity relationship for gas-phase epoxidation of propylene. Among them, the Na-Ni/TS-1 catalyst exhibits the highest catalytic activity(propylene conversion of 7.35% and PO formation rate of 157.9 g h^(-1)kg_(cat)^(-1)) and the best stability(long-term stability exceeding 140 h at 200 ℃). X-ray absorption and photoelectron spectroscopy revealed that the electronic structure of Ni can be tuned by the addition of alkali metal promoters.NH3-TPD-MS, CO_(2)-TPD-MS, and C_(3)H_(6)-TPD-MS results indicate that the acidity of the catalyst can also be adjusted by the introduction of alkali metal, whereas the Na-Ni/TS-1 catalyst exhibits the strongest C_(3)H_(6) adsorption capacity. Thus, the suitable acid-base properties, unique electronic properties of Ni species, and the strongest propylene adsorption capacity resulted in improved propylene gas-phase epoxidation activity of Na-Ni/TS-1 catalyst. This study not only provides a new strategy for the practical application of nickel-based catalysts in the gas-phase epoxidation of propylene but also provides insights into the promoting effect of alkali metals.

DIW1 encoding a cladeⅠPP2C phosphatase negatively regulates drought tolerance by de-phosphorylating TaSnRK1.1 in wheat

Drought seriously impacts wheat production(Triticum aestivum L.),while the exploitation and utilization of genes for drought tolerance are insufficient.Leaf wilting is a direct reflection of drought tolerance in plants.Clade A PP2Cs are abscisic acid(ABA)co-receptors playing vital roles in the ABA signaling pathway,regulating drought response.However,the roles of other clade PP2Cs in drought tolerance,especially in wheat,remain largely unknown.Here,we identified a gain-of-function drought-induced wilting 1(DIW1)gene from the wheat Aikang 58 mutant library by map-based cloning,which encodes a cladeⅠprotein phosphatase 2C(TaPP2C158)with enhanced protein phosphatase activity.Phenotypic analysis of overexpression and CRISPR/Cas9 mutant lines demonstrated that DIW1/TaPP2C158 is a negative regulator responsible for drought resistance.We found that TaPP2C158 directly interacts with TaSnRK1.1 and de-phosphorylates it,thus inactivating the TaSnRK1.1–Ta AREB3 pathway.TaPP2C158 protein phosphatase activity is negatively correlated with ABA signaling.Association analysis suggested that C-terminal variation of TaPP2C158 changing protein phosphatase activity is highly correlated with the canopy temperature,and seedling survival rate under drought stress.Our data suggest that the favorable allele with lower phosphatase activity of TaPP2C158 has been positively selected in Chinese breeding history.This work benefits us in understanding the molecular mechanism of wheat drought tolerance,and provides elite genetic resources and molecular markers for improving wheat drought tolerance.

Recent advances in the development of perovskite@metal-organicframeworks composites

Metal-organic frameworks(MOFs)have received considerable attention because of their advantages of adjustable structure,high porosity,and rich active centers.Meanwhile,perovskite has attracted research interest due to its unique highemission quantum yield and excellent optoelectronic properties.However,the instability of perovskites under certain conditions hinders their more comprehensive development.A novel strategy encapsulates perovskite in the pores of MOFs to protect it from external interference and increase the active centers,thus improving the performance of perovskite@MOF composites.In this review,the latest research progress in the synthesis strategy,function,and application of perovskite@MOF composites is systematically summarized.Additionally,the challenges of further developing perovskite@MOF composites are discussed.Hopefully,this review provides creative inspiration to advance future studies on perovskite@MOF composites in this emerging field.

Hot-melt extrusion promotes dissolution,extends“spring-parachute”process and inhibits crystallization in supersaturating microparticle systems

Despite the potential advantages of amorphism-induced supersaturation,the merit of new amorphiza-tion formation methods on the properties of the amorphous drug including the stability of the amor-phous state,dissolution/solubility,supersaturation,and"spring-parachute"process is still poorly understood,particularly for certain amorphous supersaturating drug delivery systems(aSDDS).The present work aimed to explore the detailed merit of current attractive amorphization manufacturing methods(i.g.,hot-melt extrusion(HME)technique)on the property improvement of aSDDS in form of amorphous solid dispersion microparticles by employing a model Bcs II drug nitrendipine and a polyvinylpyrrolidone-based model polymer copovidone.Many asDDS systems were developed by various methods,and their physicochemical properties were characterized by SEM,PXRD and DSC.HME-triggered amorphization induced superior supersaturation by the observation of the highest dissolution and solubility.HME induced the optimal supersaturation duration by the observed greatest extension of"spring-parachute"process(e.g,maximum AUCspring-parachute).HME technique is comparable with other techniques for the stabilization of amorphous state during storage.All aSDDS systems by HME and other methods showed improved long-term stability of the amorphous state in comparison to the pure amorphous drug.Fourier transformation infrared spectroscopy,Noyes-Whitney equation,nucleation theory and Gibbs free energy of transfer(△G)were used to analyze the underlying mechanisms.Mo-lecular mechanism studies indicated that HME caused a stronger crystallization inhibition effect in the asDDS systems than other methods,but molecular interaction is not a dominant mechanism for property enhancement caused by HME.For the mechanism associated with the polymer itself(PVPVA64),it could inhibit the drug recrystallization,solubilize the drug spontaneously and cause the improved molecular interactions in all aSDDS systems.This study provided a deep insight into detailed advantage of HME-triggered supersaturation/amorphization and facilitated the applications of the technique both in the field of particuology and in pharmaceutical industry.

Improving pathway prediction accuracy of constraints-based metabolic network models by treating enzymes as microcompartments

Metabolic network models have become increasingly precise and accurate as the most widespread and practical digital representations of living cells.The prediction functions were significantly expanded by integrating cellular resources and abiotic constraints in recent years.However,if unreasonable modeling methods were adopted due to a lack of consideration of biological knowledge,the conflicts between stoichiometric and other constraints,such as thermodynamic feasibility and enzyme resource availability,would lead to distorted predictions.In this work,we investigated a prediction anomaly of EcoETM,a constraints-based metabolic network model,and introduced the idea of enzyme compartmentalization into the analysis process.Through rational combination of reactions,we avoid the false prediction of pathway feasibility caused by the unrealistic assumption of free intermediate metabolites.This allowed us to correct the pathway structures of L-serine and L-tryptophan.A specific analysis explains the application method of the EcoETM-like model and demonstrates its potential and value in correcting the prediction results in pathway structure by resolving the conflict between different constraints and incorporating the evolved roles of enzymes as reaction compartments.Notably,this work also reveals the trade-off between product yield and thermodynamic feasibility.Our work is of great value for the structural improvement of constraints-based models.

A photo-elutable and template-free isothermal amplification strategy for sensitive fluorescence detection of 5-formylcytosine in genomic DNA

5-Formylcytosine(5fC), as an important epigenetic modification, plays a vital role in diverse biological processes and multiple diseases by regulating gene expression. Owing to the extremely low abundance of 5fC in all mammalian tissues and high structural similarity with other cytosine derivatives, the precise and sensitive detection of 5fC is challenging. Herein, a photo-elutable and template-free isothermal amplification strategy has been proposed for the sensitive detection of 5fC in genomic DNA based on5fC-specific biotinylation, enrichment, photocleavage, and terminal deoxynucleotidyl transferase(Td T)-assisted fluorescence signal amplification, which is termed 5fC-PTIAS. By introducing the highly specific chemolabeling and the one-step photoelution processes, this strategy possesses a minimal nonspecific background as well as a much higher amplification efficiency. With the high signal-to-noise ratio, this strategy can achieve the accurate quantification of 5fC in various biological samples including mouse brain, kidney, and liver, with a limit of detection(LOD) of 0.025‰ in DNA(S/N=3). These results not only confirm the widespread distribution of 5fC but also indicate its significant variation in different tissues and ages. The bisulfite-and mass spectrometry-free strategy is highly sensitive, selective, and easily mastered, holding great promise in detecting other epigenetic modifications with much lower levels.

距离矢量路由混合SDN网络的一致性更新

混合SDN网络作为SDN网络与传统网络的过渡形态,在保留传统网络服务的同时,兼有部分软件定义网络易于管理维护的特点,但混合SDN网络的更新问题一直没能找到有效的解决方法,特别是当距离矢量路由参与混合的情况.本文将分段路由技术引入混合SDN网络更新,提出了一种分段路由在混合SDN网络更新的应用方案,结合顺序更新与两步复制机制,设计LFCA算法.LFCA算法首先尝试用分段路由拼接最终路径并将最终路径信息封装入数据包包头.而对于不可拼接的数据流,LFCA算法构建了顾及更新远端效应的动态拓扑集并基于回溯原理来评估每一步更新操作,通过不断回溯比较来最终找到最长一致性更新序列,对于仍未完成更新的节点利用两步复制机制完成更新.经过实验验证,与之前算法相比,LFCA算法可以极大地节省算法时间开销与存储资源开销.

Generation of B Cell-Deficient Pigs by Highly Efficient CRISPR/Cas9-Mediated Gene Targeting

Generating B cell-deficient mutant is the first step to produce human antibody repertoires in large animal models. In this study, we applied the clustered regularly interspaced short palindromic repeat （CRISPR）/CRISPR-associated （Cas） system to target the JH region of the pig IgM heavy chain gene which is crucial for B cell development and differentiation. Transfection of IgM-targeting Cas9 plasmid in primary porcine fetal fibroblasts （PFFs） enabled inducing gene knock out （KO） in up to 53.3% of colonies analyzed, a quarter of which harbored biallelic modification, which was much higher than that of the traditional homologous recombination （HR）. With the aid of somatic cell nuclear transfer （SCNT） technology, three piglets with the biallelic lgM heavy chain gene mutation were produced. The piglets showed no antibody-producing B cells which indicated that the biallelic mutation of the lgM heavy chain gene effectively knocked out the function of the IgM and resulted in a B cell-deficient phenotype. Our study suggests that the CRISPR/Cas9 system combined with SCNT technology is an efficient genome-editing approach in pigs.