The interest in curtailing environmental pollution issues through physical separation processes has inspired an extensive search for novel nanoporous materials with exceptional adsorption capabilities.Covalent triazin...The interest in curtailing environmental pollution issues through physical separation processes has inspired an extensive search for novel nanoporous materials with exceptional adsorption capabilities.Covalent triazine frameworks(CTFs),emerged as a class of crystalline covalent organic frameworks(COFs),have been widely examined for various separation applications,owing to their large porosity,high stability,and rich nitrogen(N)doping.The development of CTFs for efficient adsorption of mercury(Ⅱ)(Hg^(2+))is of great importance for the field,whereas it is rarely attempted,on account of limited synthetic strategies and unknown structural-property relations of conventional CTFs derived from ionothermal approaches.Herein,we report rational synthesis of a crystalline CTF with methylthio pendant arms for efficient removal of Hg^(2+)with an exceptional capacity of 751 mg·g^(-1),ranking at the top among previously-reported adsorbents.This work may open up new possibility in the synthesis of COFs for various separations.展开更多
目的:探讨核因子E2相关因子2(Nrf2)对小鼠神经干细胞(NSCs)增殖和分化能力的影响。方法:分离新生小鼠海马组织,培养NSCs,采用电穿孔法将Nrf2 siRNA序列转染至NSCs,分别用real time RT-PCR和Western Blot检测敲减效率;运用BrdU掺入实验、...目的:探讨核因子E2相关因子2(Nrf2)对小鼠神经干细胞(NSCs)增殖和分化能力的影响。方法:分离新生小鼠海马组织,培养NSCs,采用电穿孔法将Nrf2 siRNA序列转染至NSCs,分别用real time RT-PCR和Western Blot检测敲减效率;运用BrdU掺入实验、Tuj1免疫荧光染色和CCK-8法检测Nrf2靶向siRNA对NSCs的增殖、分化能力和细胞活力的影响。通过脑立体定位技术,在成年小鼠海马部位注射Nrf2抑制剂Brusatol,应用Western Blot检测Nrf2的蛋白表达水平;通过Ki67、DCX免疫荧光染色检测下调Nrf2对海马神经发生的影响。利用活性氧簇(ROS)水平测定法和real time RT-PCR分别检测活性氧水平和氧化应激相关通路超氧化物歧化酶(SOD1和SOD2)的表达。结果:Nrf2的siRNA序列转染至NSCs,real time RT-PCR结果显示siRNA的敲减效率为(54.56±7.05)%(P<0.01),Western Blot结果显示siRNA的敲减效率为(39.50±7.90)%(P<0.01);与NC-siRNA组相比,Nrf2-siRNA组的BrdU阳性率、细胞活力均下降(P<0.01),1%胎牛血清诱导分化后,Nrf2-siRNA组Tuj1阳性率下降(P<0.01);同时Nrf2-siRNA组SOD1和SOD2的mRNA水平均较NC-siRNA组下降(P<0.05),而ROS水平较NC-siRNA组升高(P<0.05)。脑内注射Nrf2抑制剂Brusatol后,与Control组相比,Brusatol组的海马组织Nrf2蛋白表达水平降低(P<0.01);Brusatol组Ki67阳性、DCX阳性细胞数量均减少(P<0.05和P<0.01);此外Brusatol组SOD1 mRNA和SOD2水平均较Control组下降(P<0.05),而ROS水平较Control组升高(P<0.05)。结论:Nrf2基因敲减导致NSCs氧化应激水平升高,进而抑制了NSCs增殖和分化能力。展开更多
毛豆,又称菜用大豆(Glycine max L.),其味道鲜香营养价值高,是一种重要的豆科蔬菜。在减施化肥条件下,腐殖酸作为替代化肥的有机肥料的最佳选择之一。本试验探讨腐殖酸水溶肥对毛豆生长的影响,用早熟毛豆品种沪宁95-1毛豆作为试验材料,...毛豆,又称菜用大豆(Glycine max L.),其味道鲜香营养价值高,是一种重要的豆科蔬菜。在减施化肥条件下,腐殖酸作为替代化肥的有机肥料的最佳选择之一。本试验探讨腐殖酸水溶肥对毛豆生长的影响,用早熟毛豆品种沪宁95-1毛豆作为试验材料,设置3次重复,分析0 g/L(T0),1 g/L(T1),2 g/L(T2)腐殖酸水溶肥浓度下对毛豆的发芽率、株高、叶绿素等含量的影响。结果表明,T1处理的发芽率比对照增加了15.0%,T1和T2处理的株高分别比对照增加了12.8%和6.8%,其中,T1处理有显著性促进效果;T1处理单株荚数、单株荚重和百粒重均显著高于对照,分别比对照增加42.3%、48.6%和20.7%。叶绿素含量和对照相比分别增加了12.7%和6.7%;T1和T2处理均显著性地促进了可溶性糖含量,分别增加了35.3%和63.5%。结果显示,施加1 g/L浓度的腐殖酸水溶肥可以提高毛豆的发芽率、株高、叶绿素含量和可溶性糖含量。展开更多
基金The National Natural Science Foundation of China(22078349,22005319,52170109)Self-deployment Program from Lanzhou Institute of Chemical Physics(E30159SQ).
文摘The interest in curtailing environmental pollution issues through physical separation processes has inspired an extensive search for novel nanoporous materials with exceptional adsorption capabilities.Covalent triazine frameworks(CTFs),emerged as a class of crystalline covalent organic frameworks(COFs),have been widely examined for various separation applications,owing to their large porosity,high stability,and rich nitrogen(N)doping.The development of CTFs for efficient adsorption of mercury(Ⅱ)(Hg^(2+))is of great importance for the field,whereas it is rarely attempted,on account of limited synthetic strategies and unknown structural-property relations of conventional CTFs derived from ionothermal approaches.Herein,we report rational synthesis of a crystalline CTF with methylthio pendant arms for efficient removal of Hg^(2+)with an exceptional capacity of 751 mg·g^(-1),ranking at the top among previously-reported adsorbents.This work may open up new possibility in the synthesis of COFs for various separations.
文摘目的:探讨核因子E2相关因子2(Nrf2)对小鼠神经干细胞(NSCs)增殖和分化能力的影响。方法:分离新生小鼠海马组织,培养NSCs,采用电穿孔法将Nrf2 siRNA序列转染至NSCs,分别用real time RT-PCR和Western Blot检测敲减效率;运用BrdU掺入实验、Tuj1免疫荧光染色和CCK-8法检测Nrf2靶向siRNA对NSCs的增殖、分化能力和细胞活力的影响。通过脑立体定位技术,在成年小鼠海马部位注射Nrf2抑制剂Brusatol,应用Western Blot检测Nrf2的蛋白表达水平;通过Ki67、DCX免疫荧光染色检测下调Nrf2对海马神经发生的影响。利用活性氧簇(ROS)水平测定法和real time RT-PCR分别检测活性氧水平和氧化应激相关通路超氧化物歧化酶(SOD1和SOD2)的表达。结果:Nrf2的siRNA序列转染至NSCs,real time RT-PCR结果显示siRNA的敲减效率为(54.56±7.05)%(P<0.01),Western Blot结果显示siRNA的敲减效率为(39.50±7.90)%(P<0.01);与NC-siRNA组相比,Nrf2-siRNA组的BrdU阳性率、细胞活力均下降(P<0.01),1%胎牛血清诱导分化后,Nrf2-siRNA组Tuj1阳性率下降(P<0.01);同时Nrf2-siRNA组SOD1和SOD2的mRNA水平均较NC-siRNA组下降(P<0.05),而ROS水平较NC-siRNA组升高(P<0.05)。脑内注射Nrf2抑制剂Brusatol后,与Control组相比,Brusatol组的海马组织Nrf2蛋白表达水平降低(P<0.01);Brusatol组Ki67阳性、DCX阳性细胞数量均减少(P<0.05和P<0.01);此外Brusatol组SOD1 mRNA和SOD2水平均较Control组下降(P<0.05),而ROS水平较Control组升高(P<0.05)。结论:Nrf2基因敲减导致NSCs氧化应激水平升高,进而抑制了NSCs增殖和分化能力。
