Three-dimensional porous bimetallic metal–organic framework/gelatin aerogels: A readily recyclable peroxymonosulfate activator for efficient and continuous organic dye removal

As promising catalysts for the degradation of organic pollutants,metal–organic frameworks(MOFs)often face limitations due to the particle agglomeration and challenging recovery in liquid-catalysis application,stemming from their powdery nature.Engineering macroscopic structures from pulverous MOF is thus of great importance for broadening their practical applications.In this study,three-dimensional porous MOF aerogel catalysts were successfully fabricated for degrading organic dyes by activating peroxymonosulfate(PMS).MOF/gelatin aerogel(MOF/GA)catalysts were prepared by directly integrating bimetallic FeCo-BDC with gelatin solutions,followed by freeze-drying and low-temperature calcination.The FeCo-BDC-0.15/GA/PMS system exhibited remarkable performance in degrading various organic dyes,eliminating 99.2%of rhodamine B within a mere 5 min.Compared to the GA/PMS system,there was over a 300-fold increase in the reaction rate constant.Remarkably,high removal efficiency was maintained across varying conditions,including different solution pH,co-existing inorganic anions,and natural water matrices.Radical trapping experiments and electron paramagnetic resonance analysis revealed that the degradation involved radical(SO_(4)^(-)·)and non-radical routes(^(1)O_(2)),of which ^(1)O_(2) was dominant.Furthermore,even after a continuous 400-min reaction in a fixed-bed reactor at a liquid hourly space velocity of 27 h^(-1),the FeCo-BDC/GA composite sustained a degradation efficiency exceeding 98.7%.This work presents highly active MOF-gelatin aerogels for dye degradation and expands the potential for their large-scale,continuous treatment application in organic dye wastewater management.

Multi-Scale Design and Optimization of Composite Material Structure for Heavy-Duty Truck Protection Device

In this paper,to present a lightweight-developed front underrun protection device(FUPD)for heavy-duty trucks,plain weave carbon fiber reinforced plastic(CFRP)is used instead of the original high-strength steel.First,the mechanical and structural properties of plain carbon fiber composite anti-collision beams are comparatively analyzed from a multi-scale perspective.For studying the design capability of carbon fiber composite materials,we investigate the effects of TC-33 carbon fiber diameter(D),fiber yarn width(W)and height(H),and fiber yarn density(N)on the front underrun protective beam of carbon fiber compositematerials.Based on the investigation,a material-structure matching strategy suitable for the front underrun protective beam of heavy-duty trucks is proposed.Next,the composite material structure is optimized by applying size optimization and stack sequence optimization methods to obtain the higher performance carbon fiber composite front underrun protection beam of commercial vehicles.The results show that the fiber yarn height(H)has the greatest influence on the protective beam,and theH1matching scheme for the front underrun protective beamwith a carbon fiber composite structure exhibits superior performance.The proposed method achieves a weight reduction of 55.21% while still meeting regulatory requirements,which demonstrates its remarkable weight reduction effect.

基于MATLAB GUI的正交试验设计与分析系统

在工程建设的各个阶段,为了减少试验次数,在较短的时间内达到预期的试验目的,近年来,正交试验设计是最常用的试验设计方法之一。但是正交试验法用在初步筛选时收敛速度缓慢、难于确定数据变化规律,增加试验次数、试验工作烦琐、费用昂贵的情况更显突出。最终设计完成了一个“正交试验设计与分析”软件。使用者根据自己试验内容,输入相关信息,即可生成对应正交试验方案。当试验内容完成之后,输入相关试验结果,即可自主分析得到试验结果。

TAZ促进胃癌中血管生成及相关机制的研究

目的:探究Hippo通路关键效应分子TAZ在胃癌组织中的表达及其在胃癌血管生成中的作用。方法:通过免疫组织化学法分析150例胃癌组织标本中TAZ和β-catenin的表达情况;将TAZ过表达质粒及干扰质粒通过慢病毒分别转染至胃癌细胞系MGC803和MKN28中,通过细胞功能实验检测内皮细胞成管、增殖及迁移能力;使用Western blot法检测转染后的胃癌细胞中TAZ及β-catenin的表达情况;采用酶联免疫吸附试验(ELISA)检测TAZ转染后血管内皮生长因子(vascular endothelial growth factor,VEGF)蛋白表达的变化。结果:免疫组织化学法结果显示150例胃癌组织中,TAZ阳性表达64例(阳性率43%),主要定位于细胞核,其表达与肿瘤分级、TNM分期、转移及微血管密度(microvessel density,MVD)有关(P<0.05)。此外,在TAZ阳性组中β-catenin阳性表达率为67.2%,明显高于TAZ阴性组,TAZ的表达与β-catenin呈正相关。在MKN28细胞系中上调TAZ的表达,与HUVEC细胞共培养后增强了内皮细胞增殖及管道形成能力,此外还通过促进β-catenin的表达,增强了内皮细胞的迁移能力;相反,在MGC803细胞系中下调TAZ的表达,与HUVEC共培养后减弱了内皮细胞增殖和管道形成能力,此外还通过降低β-catenin的表达,抑制了内皮细胞的迁移能力。结论:胃癌细胞TAZ的高表达可能通过促进β-catenin和VEGF的表达,进而增强胃癌血管生成能力。

趋化因子CXCL5在肾癌中的表达及临床意义

目的:明确趋化因子配体5(CXC-chemokine ligand 5,CXCL5)在肾癌中的表达情况,探讨其与临床病理特征的关系以及在肿瘤预后评估中的意义。方法:通过检索Cbio Portal数据库,分析CXCL5表达与肾癌进展和预后的关系。收集2012年11月至2014年6月天津医科大学肿瘤医院收治的105例肾癌患者的临床病理资料,62例组织标本中CXCL5表达患者作为试验组,选择正常肾脏和癌旁组织标本各20例作为对照组,采用免疫组织化学染色和实时定量RT-PCR法评价。根据CXCL5表达水平将患者分为高、中、低表达组,分析各组的表达情况与临床病理特征之间的关系,利用Kaplan-Meier生存分析评价CXCL5表达与患者术后生存率的关系。结果:数据库中的结果显示,CXCL5突变患者的总生存期与无病生存期明显缩短(P<0.05),疾病复发风险明显增加(P<0.05);而且,CXCL5 mRNA表达水平与肿瘤转移、分期、阳性淋巴结数量、患者总生存状态均有一定相关性。免疫组织化学法结果显示,肾癌组织中CXCL5的表达明显高于正常肾脏组织和癌旁组织(P<0.05);亚组分析显示,CXCL5的表达水平与临床分期、Fuhrman分级呈正相关(P<0.05)。此外,表达CXCL5的肾癌患者5年生存率(65%)明显低于不表达CXCL5的患者(79%),两者比较差异具有统计学意义(P<0.05)。结论:CXCL5在肾癌中高表达,其表达水平与临床分期和病理分级呈正相关,且与不良预后相关。因此,CXCL5有望成为判断肾癌预后的预测指标和新的治疗靶点。

人工智能在肿瘤组织病理标志物分析中的应用进展

肿瘤个体化治疗的进展对肿瘤组织病理标记物的精确诊断提出了更高的要求。数字病理(digital pathology,DP)的发展为人工智能(artificial intelligence,AI)辅助诊断在肿瘤组织病理图像分析中的应用提供了基础。基于卷积神经网络的深度学习(deep learning,DL)算法能够将DP图像与计算机分析技术相结合,有望成为定量评价肿瘤组织生物标志物的重要工具。本文概述了AI在组织病理学中的发展,并以目前研究相对广泛且与临床诊疗密切相关的分子病理指标Her-2、Ki-67及PD-L1的图像分析为具体案例,重点阐述了当前AI在肿瘤病理标志物分析中的研究进展。AI辅助的肿瘤病理诊断具有客观性强及可重复性高等优点,能够实现肿瘤组织标志物诊断的定量分析,从而克服病理医生人工判读的挑战,提高病理诊断的精确性。通过计算机工具构建肿瘤组织标志物的AI判读模式,是构建未来肿瘤智能诊疗体系的重要环节。

The calmodulin-dependent protein kinase II inhibitor KN-93 protects rat cerebral cortical neurons from N-methyl-D-aspartic acid-induced injury

In this study, primary cultured cerebral cortical neurons of Sprague-Dawley neonatal rats were treated with 0.25, 0.5, and 1.0 μM calmodulin-dependent protein kinase II inhibitor KN-93 after 50 μM N-methyI-D-aspartic acid-induced injury. Results showed that, compared with N-methyi-D- aspartic acid-induced injury neurons, the activity of cells markedly increased, apoptosis was significantly reduced, leakage of lactate dehydrogenase decreased, and intracellular Ca2＋ concentrations in neurons reduced after KN-93 treatment. The expression of caspase-3, phosphorylated calmodulin-dependent protein kinase II and total calmodulin-dependent protein kinase II protein decreased after KN-93 treatment. And the effect was apparent at a dose of 1.0 pM KN-93. Experimental findings suggest that KN-93 can induce a dose-dependent neuroprotective effect, and that the underlying mechanism may be related to the down-regulation of caspase-3 and calmodulin- dependent protein kinase II expression.

ENO1 expression and Erk phosphorylation in PDAC and their effects on tumor cell apoptosis in a hypoxic microenvironment

Objective: Hypoxia is an important feature of pancreatic ductal adenocarcinoma(PDAC). Previously, we found that hypoxia promotes ENO1 expression and PDAC invasion. However, the underlying molecular mechanism was remains unclear.Methods: The relationship between ENO1 expression and clinicopathological characteristics was analyzed in 84 patients with PADC. The effects of CoCl2-induced hypoxia and ENO1 downregulation on the apoptosis, invasion, and proliferation of PDAC cells were evaluated in vitro and in vivo. Hypoxia-and ENO1-induced gene expression was analyzed by transcriptomic sequencing.Results: The prognosis of PDAC with high ENO1 expression was poor(P < 0.05). High ENO1 expression was closely associated with histological differentiation and tumor invasion in 84 PDAC cases(P < 0.05). Hypoxia increased ENO1 expression in PDAC and promoted its migration and invasion. Apoptotic cells and the apoptosis marker caspase-3 in the CoCl_(2)-treated ENO1-sh group were significantly elevated(P < 0.05). Transcriptomic sequencing indicated that CoCl_(2)-induced PDAC cells initiated MAPK signaling. Under hypoxic conditions, PDAC cells upregulated ENO1 expression, thereby accelerating ERK phosphorylation and inhibiting apoptosis(P < 0.05). Consistent results were also observed in a PDAC-bearing mouse hindlimb ischemia model.Conclusions: Hypoxia-induced ENO1 expression promotes ERK phosphorylation and inhibits apoptosis, thus leading to PDAC survival and invasion. These results suggest that ENO1 is a potential therapeutic target for PDAC.

Tuning redox activity through delithiation induced protective layer and Fe-O coordination for Li-rich cathode with improved voltage and cycle performance

Li-rich layered transition metal oxides are one of the most promising cathode materials for their high energy density.However,the cathodes usually suffer from severe potential dropping and capacity fading during cycling,which are associated with the surface oxygen release and accompanied by cation densification and structural collapse.Herein,an integrative approach of simultaneous constructing uniform 3d Fe-ion doping in the transition metal layer and Li-rich Li_(5)FeO_(4) shell to grab the oxygen and prevent interfacial side reactions is proposed.The introduction of Fe induces higher redox potential and stronger 3 d Fe-O_(2)p covalent bond,triggering reversible anionic redox via a reductive coupling mechanism.And the delithiated product of Li-rich Li_(5)FeO_(4) not only acts as a protective layer alleviating the side reactions but also enhances the surface kinetic property.With the benefit of promoted reversibility of oxygen redox and enhanced surface stability,the cathode exhibits high reversible capacity and superior cycle performance.Density function theory calculation indicates that the O_(2)p non-bonding state in the cathode incorporated with Fe sits at a lower energy band,resulting in higher energy storage voltage and improved oxygen stability.Consequently,the modified cathode exhibits a discharge specific capacity of 307 m A h g^(-1)(1 C=250 m A g^(-1)),coulombic efficiency of 82.09%in the initial cycle at 0.1 C and 88.34%capacity retention after 100 cycles at 1 C.The work illustrates a strategy that could simultaneously enhance oxygen redox reversibility and interface stability by constructing lattice bond coordination and delithiation induced protective layer to develop Li-rich materials with high reversible capacity and long lifespan.

Approximate Solutions to the Discontinuous Riemann-Hilbert Problem of Elliptic Systems of First Order Complex Equations

Several approximate methods have been used to find approximate solutions of elliptic systems of first order equations. One common method is the Newton imbedding approach, i.e. the parameter extension method. In this article, we discuss approximate solutions to discontinuous Riemann-Hilbert boundary value problems, which have various applications in mechanics and physics. We first formulate the discontinuous Riemann-Hilbert problem for elliptic systems of first order complex equations in multiply connected domains and its modified well-posedness, then use the parameter extensional method to find approximate solutions to the modified boundary value problem for elliptic complex systems of first order equations, and then provide the error estimate of approximate solutions for the discontinuous boundary value problem.

The Influence Law of Oil Relative Permeability on Water Cut

Water cut is a key evaluation parameter for reservoir development evaluation. Relative permeability curve reflects reservoir characteristics and fluid characteristics. It is important to figure out the influence law of oil relative permeability on water cut. Based on the 269 relative permeability curves of Bohai oilfields, the distribution of oil index of Bohai oilfields were studied. On the basis, combined with Corey expression of relative permeability and fractional flow equation, the theoretical relationship between oil index and water cut increasing rate was established. Three end points of water cut increasing rate curve were proposed and the influence law between three end points and oil index was studied. The results show that the oil index has a linear relationship with three end points. When the value of water oil mobile ratio is large than 1, with the increase of oil index, maximum value of water cut increasing rate gradually increase. When the value of water oil mobile ratio is less than 10, oil index has great effect on recovery percent when water cut increasing rate reaches to the maximum value as well as water cut when water cut increasing rate reaches to the maximum value. The application of SS field shows that the theoretical value is consistent with the field data.

Regulation of MRI contrast and cellular retention time by cellular interfacial distribution of Gd agents:Implications for stem cell tracking

Human mesenchymal stem cells(hMSCs)were labeled with Dotarem or(Gd-DOTA)2-EM7(EM7Gd2)via electroporation(EP).Cellular transmission electron microscopy(TEM)reveals free distribution of Gd agents and formation of EM7Gd2 clusters in the cytosol.Cellular magnetic resonance imaging(MRI)reveals that the free Gd agents induce MRI signal enhancement effect due to its fast exocytosis and subsequent interaction with intercellular water molecules.The EM7Gd2 clusters exhibits a longer intracellular retention time and induce a persistent MRI signal reduction effect.The cellular MRI results are interpreted by taking into account both T1 and T2 relaxation rates and their correlation with cellular binding structures of Dotarem and EM7Gd2.

The role of SLC12A family of cation-chloride cotransporters and drug discovery methodologies

The solute carrier family 12(SLC12)of cation-chloride cotransporters(CCCs)comprises potassium chloride cotransporters(KCCs,e.g.KCC1,KCC2,KCC3,and KCC4)-mediated Cl^(-)extrusion,and sodium potassium chloride cotransporters(N[K]CCs,NKCC1,NKCC2,and NCC)-mediated Cl^(-)loading.The CCCs play vital roles in cell volume regulation and ion homeostasis.Gain-of-function or loss-of-function of these ion transporters can cause diseases in many tissues.In recent years,there have been considerable advances in our understanding of CCCs'control mechanisms in cell volume regulations,with many techniques developed in studying the functions and activities of CCCs.Classic approaches to directly measure CCC activity involve assays that measure the transport of potassium substitutes through the CCCs.These techniques include the ammonium pulse technique,radioactive or nonradioactive rubidium ion uptakeassay,and thallium ion-uptake assay.CCCs'activity can also be indirectly observed by measuring gaminobutyric acid(GABA)activity with patch-clamp electrophysiology and intracellular chloride concentration with sensitive microelectrodes,radiotracer^(36)Cl^(-),and fluorescent dyes.Other techniques include directly looking at kinase regulatory sites phosphorylation,flame photometry,22Nat uptake assay,structural biology,molecular modeling,and high-throughput drug screening.This review summarizes the role of CCCs in genetic disorders and cell volume regulation,current methods applied in studying CCCs biology,and compounds developed that directly or indirectly target the CCCs for disease treatments.

Quantum speed limit of the double quantum dot in pure dephasing environment under measurement

The quantum speed limit(QSL)of the double quantum dot(DQD)system has been theoretically investigated by adopting the detection of the quantum point contact(QPC)in the pure dephasing environment.The Mandelstam–Tamm(MT)type of the QSL bound which is based on the trace distance has been extended to the DQD system for calculating the shortest evolving time.The increase of decoherence rate can weaken the capacity for potential speedup(CPS)and delay the evolving process due to the frequently measurement localizing the electron in the DQD system.The system needs longer time to evolve to the target state as the enhancement of dephasing rate,because the strong interaction between pure dephasing environment and the DQD system could vary the oscillation of the electron.Increasing the dephasing rate can sharp the QSL bound,but the decoherence rate would weaken the former effect and vice versa.Moreover,the CPS would be raised by increasing the energy displacement,while the enhancement of the coupling strength between two quantum dots can diminish it.It is interesting that there has an inflection point,when the coupling strength is less than the value of the point,the increasing effect of the CPS from the energy displacement is dominant,otherwise the decreasing tendency of the CPS is determined by the coupling strength and suppress the action of the energy displacement if the coupling strength is greater than the point.Our results provide theoretical reference for studying the QSL time in a semiconductor device affected by numerous factors.

Electron transfer properties of double quantum dot system in a fluctuating environment

Using the innovative method of the additional Bloch vector,the electron transfer properties of a double quantum dot(DQD) system measured by a quantum point contact(QPC) in a fluctuating environment are investigated.The results show that the environmental noises in transverse and longitudinal directions play different roles in the dynamical evolution of the open quantum systems.Considering the DQD with symmetric energy level,the Fano factor exhibits a slight peak with the increase of transverse noise amplitude σ_(T),which provides a basis for distinguishing dynamical phenomena caused by different directional fluctuation noises in symmetric DQD structures by studying the detector output.In the case of asymmetric DQD,the dependence of a detector current involving the level displacement is distinct when increasing the transverse noise damping coefficient τ_(T) and the longitudinal noise damping coefficient τ_(ε) respectively.Meanwhile,the transverse noise damping coefficient τ_(T) could significantly reduce the Fano factor and enhance the stability of the quantum system compared with the longitudinal one.The Fano factors with stable values as the enhancement of noise amplitudes show different external influences from the detector measurement,and provide a numerical reference for adjusting the noise amplitudes in both transverse and longitudinal directions appropriately in a microscopic experimental process to offset the decoherence effect caused by the measurements.Finally,the research of average waiting time provides unique insights to the development of single electron transfer theory in the short-time limit.

Analysis of the fractal intrinsic quality in the ionization of Rydberg helium and lithium atoms

We study the fractal rhythm in the ionization of Rydberg helium and lithium atoms in an electric field by using the semiclassical method.The fractal structures present a nested relationship layer by layer in the initial launch angles of the ionized electrons versus the escape time,which is defined as the rhythm attractor,and exhibit similar rhythm endings.The gradually enhanced chaotic regions of the escape time plots tend to broaden as the scaled energy increases.In addition,the fractal rhythm changes synchronously with the oscillations of the kinetic energy spectrum.We note that the intrinsic quality of the fractal rhythm is closely related to the kinetic energy distribution,that is,the inherent dynamic properties of the Hamiltonian equations have an impact on the fractal regularities.In addition,different ionizing closed trajectories exhibit iterate properties and the inherent beauty of symmetry.Our results and analysis can not only reveal new laws in the ionization of Rydberg atoms,but also promote the establishment of the dynamic mechanism of fractals.

The Correlation between the Variation of Dissolved Inorganic Carbon Content and Cyanobacterial Blooms in Fubao Bay of Dianchi Lake

To explore variation of dissolved inorganic carbon content( DIC) caused by cyanobacterial blooms and provide a basis for formulating effective preventive and control measures of cyanobacterial blooms,the concentration of inorganic carbon and the concentration of planktonic algae were studied by sampling method,and the distribution and variation of the DIC and physicochemical factors in the ecological restoration area of Fubao Bay of Dianchi Lake were analyzed. Results indicated that the distribution of chlorophyll-a was significantly positive correlated with CO_3^(2-)( P < 0. 01) and pH values( P < 0. 05); and the distribution of chlorophyll-a was significantly negative correlated with CO_2( P <0. 01),DIC and HCO_3^-( P < 0. 05). In conclusion,the outbreak and extinction of cyanobacterial blooms is one of the important reasons for the variation of inorganic carbon form and concentration.

General Boundary Value Problems for Nonlinear Uniformly Elliptic Equations in Multiply Connected Infinite Domains

This article discusses the general boundary value problem for the nonlinear uniformly elliptic equation of second order in D (0.1) and the boundary condition,(0.2) in a multiply connected infinite domain D with the boundary T. The above boundary value problem is called Problem G. Problem G extends the work [8] in which the equation (0.1) includes a nonlinear lower term and the boundary condition (0.2) is more general. If the complex equation (0.1) and the boundary condition (0.2) meet certain assumptions, some solvability results for Problem G can be obtained. By using reduction to absurdity, we first discuss a priori estimates of solutions and solvability for a modified problem. Then we present results on solvability of Problem G.

The Study on University Literature Teaching based on Multimedia Technology

currently, the multimedia language teaching has gradually entered the university, the old and the young program, and gradually plays a great charm, and gradually influence and change the traditional language teaching. Multimedia is a new technology lived in a simple or synthesized form shown by the text, the graphics, the images, the animation as well as audio, video and other information, which processed through the computer＇s software and hardware. I apply this new technology in teaching that＇ s the multimedia teaching.

A novel method for the green utilization of waste fried oil

Waste fried oil was studied to prepare three different types of detergent such as soap,liquid soap and soap powder via saponification process.The preparation conditions of soap base were optimized by orthogonal experiment.The specific preparation processes included waste fried oil treatment,orange peel extract preparation,saponification,demoulding and drying.Results showed that the optimal con-ditions for saponification were as follows:ratio of pure waste fried oil to coconut oil=6∶4,alkali liquor(NaOH)mass fraction 30%,saponification temperature 70℃,orange peel extract concentration 15%.The mature soap was then used to make liquid soap and soap powder by surfactant(sodium dodecylbenzene sulfonate,coconut diethanol amide)addition,followed by grinding.The prepared detergent conforms to the production standard of strong decontamination ability,possesses stable performance,is gentle to skin and non-toxic.