Clinical Outcome of Internal Fixation and Fusion in the Treatment of Spinal Fractures by Paraspinal Muscular Space Approach

Objective:To explore the clinical effect of internal fixation and fusion with the paraspinal muscle gap approach in the treatment of spinal fracture patients.Methods:104 spinal fracture patients admitted to Central Hospital of TCM from October 2022 to April 2024 were selected as the study subjects and were randomly divided into the control group(n=52)and the observation group(n=52)according to the random number table method.The control group was treated with the conventional approach of internal fixation surgery,and the observation group was treated with the paraspinal muscular interspace approach of internal fixation fusion.The two groups’general data,surgical indexes,pain,lumbar spine function,and postoperative complications were observed.Results:The baseline data of the two groups of patients were not statistically significant(all P>0.05)while the intraoperative bleeding,the first postoperative time getting up from bed,and the length of hospital stay of the patients in the observation group were shorter than that of the control group(all P=0.000<0.001),and the duration of the operation was longer than that of the control group(t=2.644,P=0.010<0.05);at 3 months postoperatively,the VAS scores of the patients in the observation group were significantly lower than those in the control group(t=10.768,P=0.000<0.001),and the JOA score was higher than that of the control group(t=6.498,P=0.000<0.001);the total complication rate of patients in the observation group(3/5.77%)was significantly lower than that of the control group(12/23.08%)(χ^(2)=6.310,P=0.012<0.05).Conclusion:In the treatment of spinal fracture patients,compared with the conventional approach to internal fixation surgery,the paraspinal muscular gap approach to internal fixation and fusion treatment is less traumatic,postoperative lumbar spine function recovery is faster,and can reduce the incidence of postoperative complications.

Study on Pharmacological Effects of Calycosin in Astragali Radix

Calycosin is an important isoflavone compound in traditional Chinese medicine Astragali Radix.It not only has remarkable anti-oxidation,anti-inflammatory and anti-tumor functions,but also has the characteristics of small adverse reactions and low toxicity,so it has attracted wide attention of scholars and researchers.This paper reviewed the pharmacological effects and mechanisms of calycosin in recent years,in the hope of providing a theoretical basis for its clinical application.

Research Progress on the Biological Activity of Pectolinarigenin

Pectolinarigenin is a kind of flavonoid,which is an active ingredient in Eupatorium odoratum.Some studies have confirmed that pectolinarigenin has many biological activities,including anti-oxidation,anti-allergy,anti-inflammation,anti-tumor and so on.In this paper,the biological activity and related mechanism of pectolinarigenin were summarized in order to provide theoretical basis and reference for the further development and utilization of pectolinarigenin.

Blockchain-based continuous data integrity checking protocol with zero-knowledge privacy protection

The cloud computing technology has emerged,developed,and matured in recent years,consequently commercializing remote outsourcing storage services.An increasing number of companies and individuals have chosen the cloud to store their data.However,accidents,such as cloud server downtime,cloud data loss,and accidental deletion,are serious issues for some applications that need to run around the clock.For some mission and business-critical applications,the continuous availability of outsourcing storage services is also necessary to protect users'outsourced data during downtime.Nevertheless,ensuring the continuous availability of data in public cloud data integrity auditing protocols leads to data privacy issues because auditors can obtain the data content of users by a sufficient number of storage proofs.Therefore,protecting data privacy is a burning issue.In addition,existing data integrity auditing schemes that rely on semi-trusted third-party auditors have several security problems,including single points of failure and performance bottlenecks.To deal with these issues,we propose herein a blockchain-based continuous data integrity checking protocol with zero-knowledge privacy protection.We realize a concrete construction by using a verifiable delay function with high efficiency and proof of retrievability,and prove the security of the proposal in a random oracle model.The proposed construction supports dynamic updates for the outsourced data.We also design smart contracts to ensure fairness among the parties involved.Finally,we implement the protocols,and the experimental results demonstrate the efficiency of the proposed protocol.

Schisandrin B exerts anticancer effects on human gastric cancer cells through ROS-mediated MAPK,STAT3,and NF-κB pathways

Schisandrin B(Sch B)is a monomer with anti-cancer and anti-inflammatory effects,which are isolated from the plant Schisandra chinensis(Turcz)Baillon.We investigated the anti-gastric cancer(GC)effects of Sch B and its underlying molecular mechanisms.The Cell Counting Kit-8 assay was used to determine the effects of Sch B on the viability of GC and normal cell lines.Hoechst/propidium iodide staining and flow cytometry were used to assess the apoptosis induction of Sch B.Western blotting was used to evaluate the effects of Sch B on downstream apoptotic proteins.The DCFH-DA fluorescent probe was used to assess the regulatory effects of Sch B on reactive oxygen species(ROS)levels and related signaling pathways in GC cells.The results showed that Sch B could regulate the phosphorylation level of mitogen-activated protein kinase(MAPK)by upregulating ROS accumulation in gastric cancer cells,and then reduce the expression of nuclear factor kappa B(NF-κB)and phosphorylated transcription 3(p-STAT3).In addition,Sch B downregulated the cell cycle proteins cyclin-dependent kinase 2/4/6 and cyclin D1/E,and arrested cells in the G0/G1 phase.Moreover,it also inhibited cell migration,which was reversed with Nacetylcysteine pretreatment.In summary,Sch B has killing effects on GC cells by upregulating the production of intracellular ROS and regulating the MAPK/STAT3/NF-κB signaling pathway,leading to the migration arrest and apoptosis of GC cells.

Review on marine collagen peptides induce cancer cell apoptosis,necrosis,and autophagy by reducing oxidized free radicals

Marine collagen peptides(MCPs)are natural products prepared by hydrolyzing marine collagen protein through a variety of chemical methods or enzymes.MCPs have a range of structures and biological activities and are widely present in marine species.MCPs also have a small molecular weight,are easily modified,and absorbed by the body.These properties have attracted great interest from researchers studying antioxidant,anti-tumor,and anti-aging activities.MCPs of specific molecular weights have significant anti-tumor activity and no toxic side effects.Thus,MCPs have the potential use as anti-cancer adjuvant drugs.Free radicals produced by oxidation are closely related to human aging,cancer,arteriosclerosis,and other diseases,but their relationship with cancer is not well known.In this review,we focus on the antioxidant properties of MCPs in the treatment of cancer,highlighting their antioxidant molecular structure and potential for clinical practice.

Review of Pharmacological Effects and Mechanisms of Crotonoside

Crotonoside is an important alkaloid component in Croton tiglium.It has the effects of drastic purgation,expelling water and reducing swelling,eliminating phlegm and relieving pharynx.Studies have found that crotonoside can exert a variety of pharmacological effects such as anti-cancer,anti-inflammatory,anti-arrhythmic,and anti-acute myelogenous leukemia through a variety of ways.This paper reviewed the pharmacological effects and mechanisms of crotonoside.

Preparation Process,Pharmacological Activity and Molecular Mechanism of Valepotriate

In recent years,with the continuous development of the medicinal value of valepotriate and the great attention of scholars.The traditional extraction and purification process can no longer meet the current market standards and needs.The modern technology of valepotriate is of great significance to its application and development.This paper reviews the extraction and purification process,molding process and pharmacological activity of valepotriate,providing a theoretical basis for the research and application of valepotriate.

Pharmacological Effects and Molecular Mechanism of Osthole

Osthole has various pharmacological effects such as anti-cancer,anti-inflammation,prevention and treatment of cardiovascular diseases and neuroprotection.This paper reviews the advances in the research of the pharmacological effects and molecular mechanisms of osthole,in order to provide new ideas for further research and clinical application of osthole.

Research Progress on Biological Activity of Sciadopitysin

Sciadopitysin(SP)belongs to flavonoids.Recent studies showed that sciadopitysin has anti-tumor,anti-oxidation,treatment of osteoporosis,diabetic osteopathy,Alzheimer's disease and other effects.This study will review the biological activity and related action mechanism of the sciadopitysin in recent years,in order to provide theoretical basis and reference for the future research and drug development of the sciadopitysin.

Pharmacological Action and Molecular Mechanism of Amentoflavone

Amentoflavone(AMF)is a natural active substance extracted from Chinese herbal medicine Selaginella tamariscina,and has good anti-inflammatory,antitumor,hypoglycemic and neuroprotective pharmacological effects.This paper reviews the pharmacological action and mechanism of AMF,in order to lay a theoretical foundation for in-depth research and drug development of AMF.

Pharmacological Activities and Molecular Mechanisms of Arctigenin

Arctigenin has a variety of pharmacological effects,such as anti-inflammatory,anti-tumor,antiviral and hypoglycemic.In this paper,the pharmacological effects and mechanisms of arctigenin were reviewed,in order to provide a theoretical basis for further research and development of arctigenin.

Biological Activity and Molecular Mechanism of Bullatine G

Bullatine G has many biological effects such as anti-inflammatory,anti-anxiety,anti-tumor,anti-arrhythmia and anti-heart failure effect.In order to provide a theoretical basis for the further study and clinical application of bullatine G,this article reviews the biological activity of bullatine G in recent years.

Purification Process and Biological Activity of Daphnoretin

Daphnoretin,belonging to coumarin compounds,is the main active ingredient of Wikstroemia indica,and has anti-inflammatory,anti-depression,anti-tumor and other pharmacological activities.This article reviews the extraction and purification process,content determination methods and pharmacological activity of daphnoretin,in order to provide a theoretical reference for optimization of purification process,improvement of content determination technique and further clinical application of daphnoretin.

Optimization of Extraction, Purification and Identification Process of Bullatine G

The purification processes of bullatine G, such as acid water extraction, ammonia alkalization ether cold soaking method, ultrasonic assisted extraction and cellulase extraction, and the determination technologies of bullatine G content, such as UV-spectrophotometry, chromatography, near infrared spectroscopy (NLR), thin layer chromatography (TLC), high performance liquid chromatography-mass spectrometry (HPLC-MS) were reviewed, in order to provide a theoretical basis for the development and application of bullatine G.

Advances in Research of Pharmacological Effects of Peimine

Peimine is an important effective component of the traditional Chinese medicine Fritillariae Thunbergii Bulbus.It not only has multiple pharmacological activities such as anti-inflammatory,antitussive,analgesic and anti-cerebral ischemia-reperfusion injury,but also can play anti-cancer effects by inhibiting the proliferation of cancer cells,inducing apoptosis of cancer cells,and inhibiting the metastasis and invasion of cancer cells.The present article summarizes and reviews the pharmacological activities and action mechanism of peimine in recent years.

Study on the Anti-tumor Mechanism of Immunological Checkpoint Inhibitor PD-1

In recent years,the research of programmed death factor-1(PD-1)and its ligand(PD-L1)has made a great breakthrough in tumor therapy,and it is expected to change the clinical treatment manner of anti-tumor.In this paper,the role and mechanism of PD-1 inhibitors in anti-tumor are reviewed,thereby promoting their clinical application in anti-tumor immunotherapy.

Anti-inflammatory,Bacteriostatic and Anticancer Effects of Madecassoside

Madecassoside is a natural active component extracted from Centella asiatica.In recent years,a large number of studies have reported that madecassoside has a variety of biological activities,such as anticancer,anti-inflammatory and antibacterial effects,prevention and treatment of cardiovascular diseases,nerve damage,visceral damage and arthritis,and other pharmacological effects.In this paper,the pharmacological action and mechanism of madecassoside were reviewed to provide a theoretical basis for further research of madecassoside and drug development.

Research Progress on Purification Process Optimization and Content Determination of Zeaxanthin

At present,the purification process of zeaxanthin mainly includes organic solvent extraction,ultrasonic-assisted extraction and enzyme extraction,and the content determination technology mainly includes ultraviolet-spectrophotometry and high performance liquid chromatography.In this paper,the purification process and content determination technology of zeaxanthin in recent years are reviewed in order to provide ideas and theoretical basis for further research and application of zeaxanthin.

In silico design of anti-tumor mini-protein targeting MDM2

We designed a disulfide-crosslinked mini-protein with a two-helical topology consisting of L-and Damino acids,which was exceptionally stable in serum.Therefore,we further used it as a scaffold to design mini-proteins targeting p53 positive tumor cells.Based on bifunctional grafting,key residues from the transactivation domain of p53 and a designed unnatural amino acid were grafted into the helix constituted by L-amino acids to confer the mini-protein with MDM2 inhibitory activity.Meanwhile,ten Arg residues were introduced to improve its membrane penetrating capacity.Among the mini-proteins,UPROL-10e showed nano-molar binding affinity on MDM2 and cellular toxicity on p53 expressing HCT116cells.