Background:Glioma is a kind of tumor that easily deteriorates and originates from glial cells in nerve tissue.Honokiol is a bisphenol compound that is an essential monomeric compound extracted from the roots and bark ...Background:Glioma is a kind of tumor that easily deteriorates and originates from glial cells in nerve tissue.Honokiol is a bisphenol compound that is an essential monomeric compound extracted from the roots and bark of Magnoliaceae plants.It also has anti-infection,antitumor,and immunomodulatory effects.In this study,we found that honokiol induces cell apoptosis in the human glioma cell lines U87-MG and U251-MG.However,the mechanism through which honokiol regulates glioma cell apoptosis is still unknown.Methods:We performed RNA-seq analysis of U251-MG cells treated with honokiol and control cells.Protein-protein interaction(PPI)network analysis was performed,and the 10 top hub unigenes were examined via real-time quantitative PCR.Furthermore,MAPK signaling and ferroptosis were detected via western blotting.Results:332 differentially expressed genes(DEGs)were found,comprising 163 increased and 169 decreased genes.Analysis of the DEGs revealed that various biological processes were enriched,including‘response to hypoxia’,‘cerebellum development cellular response to hypoxia,’‘iron ion binding,’‘oxygen transporter activity,’‘oxygen binding,’‘ferric iron binding,’and‘structural constituent of cytoskeleton.’Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis revealed that the DEGs were enriched in the following pathways:‘mitogen-activated protein kinases(MAPK)’,‘Hypoxia-inducible factor 1(HIF-1)’,‘ferroptosis,’‘Peroxisome proliferator-activated receptor(PPAR),’‘Phosphatidylinositol-4,5-bisphosphate 3-kinase(PI3K)-protein kinase B(Akt),’and‘phagosome.’Among these pathways,the MAPK signaling pathway and ferroptosis were verified.Conclusion:This study revealed the potential mechanism by which honokiol induces apoptosis and provided a comprehensive analysis of DEGs in honokiol-treated U251-MG cells and the associated signaling pathways.These data could lead to new ideas for future research and therapy for patients with glioma.展开更多
In the present study, agar diffusion test and field trials were carried out using a mushroom (Pleurotus ostreatus) culti- var Shouyanping as the experimental material to determine the antibacterial activity of refin...In the present study, agar diffusion test and field trials were carried out using a mushroom (Pleurotus ostreatus) culti- var Shouyanping as the experimental material to determine the antibacterial activity of refined pyroligneous acid prepared from almond shell against Pseudomonas tolaasfi and its effects on mycelial growth of mushroom. The results revealed that 1.25%, 2.50% and 5.00% of refined pyroligneous acid obviously inhibited the occurrence of brown blotch disease in mushroom, but also greatly promoted mushroom mycelial growth. Therefore, 1.25% and 2.50% of refined pyroligneous acid can be used in fields to prevent brown blotch disease, and 5.00% refined pyroligneous acid can be used to control the incidence of brown blotch disease when it widely occurs in fields.展开更多
Connexin 43, a gap junction protein, is expressed mainly in glia in the central nervous system. Neuroinflammation plays an important role in central nervous system injury. Changes to glial connexin 43 levels and neuro...Connexin 43, a gap junction protein, is expressed mainly in glia in the central nervous system. Neuroinflammation plays an important role in central nervous system injury. Changes to glial connexin 43 levels and neuroinflammation may trigger brain injury and neurodegenerative diseases To illustrate the relationship between connexin 43 and neuroinflammation, this study investigated how connexin 43 expression levels change in lipopolysaccharide-stimulated rat C6 glioma cells. C6 cells were treated with 0.05, 0.25, 0.5, 1,2.5 and 5 IJg/mL lipopolysaccharide for 24 hours. The nitrite estimation-detected nitric oxide release level was elevated substantially after lipopolysaccharide stimulation. To test the transcriptional level changes of inducible nitric oxide synthase, tumor necrosis factor-a and connexin 43 mRNA, C6 cells were treated with 5 pg/mL lipopolysaccharide for 3 48 hours. Reverse transcription-PCR showed that the expression of inducible nitric oxide synthase and tumor necrosis factor-a mRNA increased over time, but connexin 43 mRNA levels increased in lipopolysaccharide-stimulated C6 cells at 3 and 6 hours, and then decreased from 12 to 48 hours. Connexin 43 protein expression was detected by immunofluorescence staining, and the protein levels matched the mRNA expression levels. These results suggest that connexin 43 expression is biphasic in lipopo^ysacchadde-induced neuroinflammation in C6 cells, which may be correlated with the connexin 43 compensatory mechanism.展开更多
[Objectives] This study was conducted to explore the antifungal activity of wood vinegar produced from apricot shell. [Methods]The minimal inhibitory concentration( MIC) of wood vinegar against Aspergillus fumigatus...[Objectives] This study was conducted to explore the antifungal activity of wood vinegar produced from apricot shell. [Methods]The minimal inhibitory concentration( MIC) of wood vinegar against Aspergillus fumigatus was determined on agar plates. Then,the mycelial growth and the morphology of the apical vesicles of A. fumigatus cultured in the medium supplemented with 1/2 MIC wood vinegar were observed to evaluate the effect of wood vinegar on A. fumigatus.RNA-Seq was performed to explore the antifungal mechanism of wood vinegar. [Results] The wood vinegar produced from apricot shell could inhibit the growth of A. fumigatus,with a MIC of 20 μl/ml. In the medium supplemented with 1/2 MIC wood vinegar,the mycelial growth of A. fumigatus was suppressed,some apical vesicles were incompletely developed. The gene ontology( GO) term enrichment analysis based on RNA-Seq showed that the differentially expressed genes( DEGs)were enriched in metabolic process,cellular process and single-organism process in the category of biological processes,cell,cell part,membrane,organelle and membrane part in the category of cellular components,catalytic activity and binding in the category of molecular function. The significantly up-regulated DEGs were mostly involved in cell,cell part,organelle,organelle part and macro molecular complex in the category of cellular component,while the significantly down-regulated DEGs were mostly involved in biological process,cellular process and single-organism process in the category of biological process,catalytic activity,binding,transporter activity,and nucleic acid binding transcription factor activity in the category molecular function. Moreover,KEGG( Kyoto Encyclopedia of Genes and Genomes) pathway analysis showed that the DEG unigenes involved in metabolism were the most,followed by those involved in genetic information processing.[Conclusions]The results indicated that the wood vinegar produced from apricot shell might inhibit the growth of A. fumigatus by interfering with the expression of multiple genes involved in multiple signaling pathways.展开更多
Cancer immunotherapy has significantly flourished and revolutionized the limited conventional tumor therapies,on account of its good safety and long-term memory ability.Discouragingly,low patient response rates and po...Cancer immunotherapy has significantly flourished and revolutionized the limited conventional tumor therapies,on account of its good safety and long-term memory ability.Discouragingly,low patient response rates and potential immune-related side effects make it rather challenging to literally bring immunotherapy from bench to bedside.However,it has become evident that,although the immunosuppressive tumor microenvironment(TME)plays a pivotal role in facilitating tumor progression and metastasis,it also provides various potential targets for remodeling the immunosuppressive TME,which can consequently bolster the effectiveness of antitumor response and tumor suppression.Additionally,the particular characteristics of TME,in turn,can be exploited as avenues for designing diverse precise targeting nanomedicines.In general,it is of urgent necessity to deliver nanomedicines for remodeling the immunosuppressive TME,thus improving the therapeutic outcomes and clinical translation prospects of immunotherapy.Herein,we will illustrate several formation mechanisms of immunosuppressive TME.More importantly,a variety of strategies concerning remodeling immunosuppressive TME and strengthening patients'immune systems,will be reviewed.Ultimately,we will discuss the existing obstacles and future perspectives in the development of antitumor immunotherapy.Hopefully,the thriving bloom of immunotherapy will bring vibrancy to further exploration of comprehensive cancer treatment.展开更多
Conventional firefighting clothing and fire masks can protect firemen’s safety to a certain extent,whereas cannot perceive environmental hazards and monitor their physical status in real time.Herein,we fabricated two...Conventional firefighting clothing and fire masks can protect firemen’s safety to a certain extent,whereas cannot perceive environmental hazards and monitor their physical status in real time.Herein,we fabricated two kinds of Janus graphene/poly(pphenylene benzobisoxazole)(PBO)fabrics by laser direct writing approach and evaluated their performance as intelligent firefighting clothes and fire masks.The results showed that the Janus graphene/PBO fabrics were virtually non-combustible and achieved the highest thermal protection time of 18.91 s ever reported in flame,which is due to the intrinsic flame-retardant nature of PBO fibers.The graphene/PBO woven fabrics-based sensor showed good repeatability and stability in human motion monitoring and NO_(2)gas detection.Furthermore,the piezoelectric fire mask was assembled with graphene/PBO nonwoven fabric as electrode layer and polyvinylidene fluoride(PVDF)electrostatic direct writing film as piezoelectric layer.The filtration efficiency of the fire mask reaches 95%for PM_(2.5)and 100%for PM_(3.0),indicating its effective filtration capability for smoke particles in fires.The respiratory resistance of the piezoelectric fire mask(46.8 Pa)was lower than that of commercial masks(49 Pa),showing that it has good wearing comfort.Besides,the piezoelectric fire mask can be sensitive to the speed and intensity of human breathing,which is essential for indirectly reflecting the health of the human body.Consequently,this work provides a facile approach to fabricate next-generation intrinsic flame-retardant smart textiles for smart firefighting.展开更多
As known,the benefits of photothermal therapy(PTT)are greatly limited by the heat tolerance of cancer cells resulting from overexpressed heat shock proteins(HSPs).Then HSPs further trigger the formation of stress gran...As known,the benefits of photothermal therapy(PTT)are greatly limited by the heat tolerance of cancer cells resulting from overexpressed heat shock proteins(HSPs).Then HSPs further trigger the formation of stress granules(SGs)that regulate protein expression and cell viability under various stress conditions.Inhibition of SG formation can sensitize tumor cells to PTT.Herein,we developed PEGylated pH(low)insertion peptide(PEG-pHLIP)-modified hollow copper sulfide nanoparticles(HCuS NPs)encapsulating the SG inhibitor ISRIB,with the phase-change material lauric acid(LA)as a gatekeeper,to construct a pH-driven and NIR photo-responsive controlled smart drug delivery system(IL@H-PP).The nano medicine could specifically target slightly acidic tumor sites.Upon irradiation,IL@H-PP realized PTT,and the light-controlled release of ISRIB could effectively inhibit the formation of PTT-induced SG to sensitize tumor cells to PTT,thereby increasing the antitumor effect and inducing potent immunogenic cell death(ICD).Moreover,IL@H-PP could promote the production of reactive oxygen species(ROS)by tumor-associated macrophages(TAMs),repolarizing them towards the M1 phenotype and remodeling the immunosuppressive microenvironment.In vitro/vivo results revealed the potential of PTT combined with SG inhibitors,which provides a new paradigm for antitumor and antimetastases.展开更多
With the rapid development of urban, the scale of the city is expanding day by day. The road environment is becoming more and more complicated. The vehicle ego-localization in complex road environment puts forward imp...With the rapid development of urban, the scale of the city is expanding day by day. The road environment is becoming more and more complicated. The vehicle ego-localization in complex road environment puts forward imperative requirements for intelligent driving technology. The reliable vehicle ego-localization, including the lane recognition and the vehicle position and attitude estimation, at the complex traffic intersection is significant for the intelligent driving of the vehicle. In this article, we focus on the complex road environment of the city, and propose a pose and position estimation method based on the road sign using only a monocular camera and a common GPS (global positioning system). Associated with the multi-sensor cascade system, this method can be a stable and reliable alternative when the precision of multi-sensor cascade system decreases. The experimental results show that, within 100 meters distance to the road signs, the pose error is less than 2 degrees, and the position error is less than one meter, which can reach the lane-level positioning accuracy. Through the comparison with the Beidou high-precision positioning system L202, our method is more accurate for detecting which lane the vehicle is driving on.展开更多
Acute myocardial infarction(AMI)remains a dominant origin of morbidity,mortality and disability worldwide.Increases in reactive oxygen species(ROS)are key contributor to excessive cardiac injury after AMI.Here we deve...Acute myocardial infarction(AMI)remains a dominant origin of morbidity,mortality and disability worldwide.Increases in reactive oxygen species(ROS)are key contributor to excessive cardiac injury after AMI.Here we developed an immobilized enzyme with Superoxide Dismutase(SOD)activity cross-link with Zr-based metal-organic framework(ZrMOF)(SOD-ZrMOF)for mitigate ROS-caused injury.In vitro and in vivo evidence indicates that SOD-ZrMOF exhibits excellent biocompatibility.By efficiently scavenging ROS and suppressing oxidative stress,SOD-ZrMOF can protect the function of mitochondria,reduce cell death and alleviate inflammation.More excitingly,long-term study using an animal model of AMI demonstrated that SOD-ZrMOF can reduce the infarct area,protect cardiac function,promote angiogenesis and inhibit pathological myocardial remodeling.Therefore,SOD-ZrMOF holds great potential as an efficacious and safe nanomaterial treatment for AMI.展开更多
Erratum to Nano Research 2023,16(5):7600-7608 https://doi.org/10.1007/s12274-023-5382-y The name of the third author in original paper was unfortunately misspelled.It should be“Huimin Wang”,instead of“Huiming Wang”.
Acetylcholinesterase(AChE) is a key enzyme used to detect organophosphorus pesticide residues by the enzyme inhibition method.An accidental discovery of a mutant strain with AChE activity was made in our laboratory du...Acetylcholinesterase(AChE) is a key enzyme used to detect organophosphorus pesticide residues by the enzyme inhibition method.An accidental discovery of a mutant strain with AChE activity was made in our laboratory during the process of AChE expression by Pichia pastoris.The pPIC9 K-Drosophila melanogaster acetylcholinesterase(DmAChE)-like expression vector was constructed by codon optimization of this mutant strain,which was transformed into P.pastoris GS115,and positive clones were selected on yeast peptone dextrose(YPD) plate with G418 at 4.0 mg/mL.The GS115-pPIC9 K-DmAChE-like strain was subjected to 0.5% methanol induction expression for 120 h,with a protein band at 4.3 kDa found by the tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) pattern of the fermentation supernatant.After preliminary purification by ammonium sulfate precipitation,the enzyme activity was detected to be 76.9 U/(mL·min).In addition,the pesticide sensitivity test proved that DmAChE-like is selective and sensitive to organophosphorus pesticides.展开更多
Two common surface-dust emission schemes using critical wind speed and friction velocity were compared with the regional climate model RegCM3 in East Asia. In the comparison, transport of mineral dust and its distribu...Two common surface-dust emission schemes using critical wind speed and friction velocity were compared with the regional climate model RegCM3 in East Asia. In the comparison, transport of mineral dust and its distribution were simulated from March to April, 2001. Simulation results were also compared with TOMS aerosol index, showing that obvious differences exist in dust emission quantity and its column burden simulated by the dust emission schemes of friction velocity and wind speed criteria. The results obtained by the wind speed criterion are higher than that by friction velocity, bringing forth the problem whether or not the dust emission scheme matches the model. The obvious difference in the two schemes also explains the uncertainty of simulating mineral dust aerosol by modeling.展开更多
1 Introduction Graphene is the first demonstration of a truly 2D atomic layer crystal, and has shown various excellent properties (e.g., ultrahigh carrier mobility, superior toughness, unique thermal conductivity, and...1 Introduction Graphene is the first demonstration of a truly 2D atomic layer crystal, and has shown various excellent properties (e.g., ultrahigh carrier mobility, superior toughness, unique thermal conductivity, and good optics performance). The family of 2D crystals has grown to include metals (e.g., NbS2, VS2, TaS2), semiconductors (e.g., MoS2, WS2, ZrS2), and insulators (e.g., hexagonal boron nitride (hBN)), many of which are stable at ambient conditions [1]. 2D layered metal dichalcogenides (LMDs) materials [2].展开更多
The results from some general circulation models show distinct radiative forcing (RF) by dust aerosol, which potentially has an effect on climate change. The direct RF and regional climatic effects of dust aerosol o...The results from some general circulation models show distinct radiative forcing (RF) by dust aerosol, which potentially has an effect on climate change. The direct RF and regional climatic effects of dust aerosol over the East Asian region are investigated in this study using NCAR's Community Atmospheric Model version 3.1. The negative RF at the top of the atmosphere (TOA) and the surface (SRF) has been revealed except for some high-albedo regions, which leads to a decrease in the surface air temperature and brings an increase of atmospheric radiative heating under both clear- and all-sky conditions. The decrease in the surface air temperature can be found over a wide region that includes the Indian peninsula and northwest China. It accompanies an increase in eastern China and the Korean peninsula, and temperature changes are not limited to regions with a large dust optical depth. A belt of vapor increase is revealed from the Indian peninsula extending east to northern China, while vapor content evidently deceases in southwest China. An increase in precipitation can also be found in the belt of increased vapor accompanying the reduction of precipitation across the regions to the south of 30~N. The pattern of rainfall change helps to offset the trend of increasing wetness in the south and increasing dryness in the north of China in recent years.展开更多
In the present paper, the rigidity of hypersurfaces in Euclidean space is revisited.The Darboux equation is highlighted and two new proofs of the rigidity are given via energy method and maximal principle, respectively.
The two most common inherited forms of colorectal cancer(CRC)are familial adenomatous polyposis and hereditary nonpolyposis colorectal cancer.They are caused by germline mutations in the APC and mismatch repair genes,...The two most common inherited forms of colorectal cancer(CRC)are familial adenomatous polyposis and hereditary nonpolyposis colorectal cancer.They are caused by germline mutations in the APC and mismatch repair genes,respectively.1 The simultaneous inheritance of both an APC gene mutation and a mismatch repair gene mutation is very rare.In this study,we reported a coinheritance of mutations in the APC and MLH1 genes in a 20-year-old patient with CRC and multiple polyposes.We described for the first time the somatic mutational profile of a colorectal tumor harboring concurrent germline mutations in the APC and MLH1 genes.展开更多
基金The study was supported by the Natural Science Foundation of Jilin Province(Grant No.20200201444JC).
文摘Background:Glioma is a kind of tumor that easily deteriorates and originates from glial cells in nerve tissue.Honokiol is a bisphenol compound that is an essential monomeric compound extracted from the roots and bark of Magnoliaceae plants.It also has anti-infection,antitumor,and immunomodulatory effects.In this study,we found that honokiol induces cell apoptosis in the human glioma cell lines U87-MG and U251-MG.However,the mechanism through which honokiol regulates glioma cell apoptosis is still unknown.Methods:We performed RNA-seq analysis of U251-MG cells treated with honokiol and control cells.Protein-protein interaction(PPI)network analysis was performed,and the 10 top hub unigenes were examined via real-time quantitative PCR.Furthermore,MAPK signaling and ferroptosis were detected via western blotting.Results:332 differentially expressed genes(DEGs)were found,comprising 163 increased and 169 decreased genes.Analysis of the DEGs revealed that various biological processes were enriched,including‘response to hypoxia’,‘cerebellum development cellular response to hypoxia,’‘iron ion binding,’‘oxygen transporter activity,’‘oxygen binding,’‘ferric iron binding,’and‘structural constituent of cytoskeleton.’Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis revealed that the DEGs were enriched in the following pathways:‘mitogen-activated protein kinases(MAPK)’,‘Hypoxia-inducible factor 1(HIF-1)’,‘ferroptosis,’‘Peroxisome proliferator-activated receptor(PPAR),’‘Phosphatidylinositol-4,5-bisphosphate 3-kinase(PI3K)-protein kinase B(Akt),’and‘phagosome.’Among these pathways,the MAPK signaling pathway and ferroptosis were verified.Conclusion:This study revealed the potential mechanism by which honokiol induces apoptosis and provided a comprehensive analysis of DEGs in honokiol-treated U251-MG cells and the associated signaling pathways.These data could lead to new ideas for future research and therapy for patients with glioma.
基金Supported by the fund of Science and Technology Department of Hebei Province(13226511)the fund of Chengde Municipal Finance Bureau(CZ2014002,CZ2013007)the fund of Chengde Municipal Science and Technology Bureau(20157029)
文摘In the present study, agar diffusion test and field trials were carried out using a mushroom (Pleurotus ostreatus) culti- var Shouyanping as the experimental material to determine the antibacterial activity of refined pyroligneous acid prepared from almond shell against Pseudomonas tolaasfi and its effects on mycelial growth of mushroom. The results revealed that 1.25%, 2.50% and 5.00% of refined pyroligneous acid obviously inhibited the occurrence of brown blotch disease in mushroom, but also greatly promoted mushroom mycelial growth. Therefore, 1.25% and 2.50% of refined pyroligneous acid can be used in fields to prevent brown blotch disease, and 5.00% refined pyroligneous acid can be used to control the incidence of brown blotch disease when it widely occurs in fields.
基金sponsored by the National Natural Science Foundation of China,No. 30901323
文摘Connexin 43, a gap junction protein, is expressed mainly in glia in the central nervous system. Neuroinflammation plays an important role in central nervous system injury. Changes to glial connexin 43 levels and neuroinflammation may trigger brain injury and neurodegenerative diseases To illustrate the relationship between connexin 43 and neuroinflammation, this study investigated how connexin 43 expression levels change in lipopolysaccharide-stimulated rat C6 glioma cells. C6 cells were treated with 0.05, 0.25, 0.5, 1,2.5 and 5 IJg/mL lipopolysaccharide for 24 hours. The nitrite estimation-detected nitric oxide release level was elevated substantially after lipopolysaccharide stimulation. To test the transcriptional level changes of inducible nitric oxide synthase, tumor necrosis factor-a and connexin 43 mRNA, C6 cells were treated with 5 pg/mL lipopolysaccharide for 3 48 hours. Reverse transcription-PCR showed that the expression of inducible nitric oxide synthase and tumor necrosis factor-a mRNA increased over time, but connexin 43 mRNA levels increased in lipopolysaccharide-stimulated C6 cells at 3 and 6 hours, and then decreased from 12 to 48 hours. Connexin 43 protein expression was detected by immunofluorescence staining, and the protein levels matched the mRNA expression levels. These results suggest that connexin 43 expression is biphasic in lipopo^ysacchadde-induced neuroinflammation in C6 cells, which may be correlated with the connexin 43 compensatory mechanism.
基金Supported by the Key Scientific and Technological Research Project for Universities of Hebei Province(ZD2018306)
文摘[Objectives] This study was conducted to explore the antifungal activity of wood vinegar produced from apricot shell. [Methods]The minimal inhibitory concentration( MIC) of wood vinegar against Aspergillus fumigatus was determined on agar plates. Then,the mycelial growth and the morphology of the apical vesicles of A. fumigatus cultured in the medium supplemented with 1/2 MIC wood vinegar were observed to evaluate the effect of wood vinegar on A. fumigatus.RNA-Seq was performed to explore the antifungal mechanism of wood vinegar. [Results] The wood vinegar produced from apricot shell could inhibit the growth of A. fumigatus,with a MIC of 20 μl/ml. In the medium supplemented with 1/2 MIC wood vinegar,the mycelial growth of A. fumigatus was suppressed,some apical vesicles were incompletely developed. The gene ontology( GO) term enrichment analysis based on RNA-Seq showed that the differentially expressed genes( DEGs)were enriched in metabolic process,cellular process and single-organism process in the category of biological processes,cell,cell part,membrane,organelle and membrane part in the category of cellular components,catalytic activity and binding in the category of molecular function. The significantly up-regulated DEGs were mostly involved in cell,cell part,organelle,organelle part and macro molecular complex in the category of cellular component,while the significantly down-regulated DEGs were mostly involved in biological process,cellular process and single-organism process in the category of biological process,catalytic activity,binding,transporter activity,and nucleic acid binding transcription factor activity in the category molecular function. Moreover,KEGG( Kyoto Encyclopedia of Genes and Genomes) pathway analysis showed that the DEG unigenes involved in metabolism were the most,followed by those involved in genetic information processing.[Conclusions]The results indicated that the wood vinegar produced from apricot shell might inhibit the growth of A. fumigatus by interfering with the expression of multiple genes involved in multiple signaling pathways.
文摘形成血管和淋巴管内层的内皮细胞是脉管系统的重要组成部分,并参与血管和淋巴系统疾病的发病机制。内皮细胞上的血管生成素(Angiopoietin,Ang)-具有免疫球蛋白和表皮生长因子同源性结构域的酪氨酸蛋白激酶(Tyrosine kinase receptors with immunoglobulin and EGF homology domains,Tie)轴是除了血管内皮生长因子受体途径外胚胎心血管和淋巴发育所必需的第二种内皮细胞特异性配体-受体信号传导系统。Ang-Tie轴参与调节产后血管生成与重塑、血管通透性和炎症,以维持血管平衡,因此,该系统在许多血管和淋巴系统疾病中发挥重要的作用。针对近年来Ang-Tie轴在血管和淋巴系统相关疾病中作用的研究进展,文中系统论述了Ang-Tie轴在炎症诱导的血管通透性、血管重塑、眼部新生脉管、剪切应力反应、动脉粥样硬化和肿瘤血管生成和转移中的作用,并总结了涉及Ang-Tie轴的相关治疗性抗体、重组蛋白和小分子药物。
基金This study was supported by National Natural Science Foundation of China(82173762)111 Project(B18035,China)the Key Research and Development Program of Science and Technology Department of Sichuan Province(2022JDJQ0050,2022YFS0334).
文摘Cancer immunotherapy has significantly flourished and revolutionized the limited conventional tumor therapies,on account of its good safety and long-term memory ability.Discouragingly,low patient response rates and potential immune-related side effects make it rather challenging to literally bring immunotherapy from bench to bedside.However,it has become evident that,although the immunosuppressive tumor microenvironment(TME)plays a pivotal role in facilitating tumor progression and metastasis,it also provides various potential targets for remodeling the immunosuppressive TME,which can consequently bolster the effectiveness of antitumor response and tumor suppression.Additionally,the particular characteristics of TME,in turn,can be exploited as avenues for designing diverse precise targeting nanomedicines.In general,it is of urgent necessity to deliver nanomedicines for remodeling the immunosuppressive TME,thus improving the therapeutic outcomes and clinical translation prospects of immunotherapy.Herein,we will illustrate several formation mechanisms of immunosuppressive TME.More importantly,a variety of strategies concerning remodeling immunosuppressive TME and strengthening patients'immune systems,will be reviewed.Ultimately,we will discuss the existing obstacles and future perspectives in the development of antitumor immunotherapy.Hopefully,the thriving bloom of immunotherapy will bring vibrancy to further exploration of comprehensive cancer treatment.
基金the National Natural Science Foundation of China(Nos.52073224 and 52202111)the Textile Vision Basic Research Program of China(No.J202110)+4 种基金the Key Research and Development Program of Xianyang Science and Technology Bureau,China(No.2021ZDYF-GY-0035)the Key Research and Development Program of Shaanxi Province,China(No.2022SF-470)the Key Research and Development Program of Shaanxi Province,China(No.2022GY-377)the Natural Science Foundation of Shaanxi Province(No.2021JQ-685)the Scientific Research Project of Shaanxi Provincial Education Department,China(No.22JC035).
文摘Conventional firefighting clothing and fire masks can protect firemen’s safety to a certain extent,whereas cannot perceive environmental hazards and monitor their physical status in real time.Herein,we fabricated two kinds of Janus graphene/poly(pphenylene benzobisoxazole)(PBO)fabrics by laser direct writing approach and evaluated their performance as intelligent firefighting clothes and fire masks.The results showed that the Janus graphene/PBO fabrics were virtually non-combustible and achieved the highest thermal protection time of 18.91 s ever reported in flame,which is due to the intrinsic flame-retardant nature of PBO fibers.The graphene/PBO woven fabrics-based sensor showed good repeatability and stability in human motion monitoring and NO_(2)gas detection.Furthermore,the piezoelectric fire mask was assembled with graphene/PBO nonwoven fabric as electrode layer and polyvinylidene fluoride(PVDF)electrostatic direct writing film as piezoelectric layer.The filtration efficiency of the fire mask reaches 95%for PM_(2.5)and 100%for PM_(3.0),indicating its effective filtration capability for smoke particles in fires.The respiratory resistance of the piezoelectric fire mask(46.8 Pa)was lower than that of commercial masks(49 Pa),showing that it has good wearing comfort.Besides,the piezoelectric fire mask can be sensitive to the speed and intensity of human breathing,which is essential for indirectly reflecting the health of the human body.Consequently,this work provides a facile approach to fabricate next-generation intrinsic flame-retardant smart textiles for smart firefighting.
基金supported by the National Natural Science Foundation of China(82173762,China)the Key Research and Development Program of Science and Technology Department of Sichuan Province(2022YFS0334,2022JDJQ0500,China)+1 种基金111 Project(B18035,China)the Fundamental of Research Funds for the Central Universities。
文摘As known,the benefits of photothermal therapy(PTT)are greatly limited by the heat tolerance of cancer cells resulting from overexpressed heat shock proteins(HSPs).Then HSPs further trigger the formation of stress granules(SGs)that regulate protein expression and cell viability under various stress conditions.Inhibition of SG formation can sensitize tumor cells to PTT.Herein,we developed PEGylated pH(low)insertion peptide(PEG-pHLIP)-modified hollow copper sulfide nanoparticles(HCuS NPs)encapsulating the SG inhibitor ISRIB,with the phase-change material lauric acid(LA)as a gatekeeper,to construct a pH-driven and NIR photo-responsive controlled smart drug delivery system(IL@H-PP).The nano medicine could specifically target slightly acidic tumor sites.Upon irradiation,IL@H-PP realized PTT,and the light-controlled release of ISRIB could effectively inhibit the formation of PTT-induced SG to sensitize tumor cells to PTT,thereby increasing the antitumor effect and inducing potent immunogenic cell death(ICD).Moreover,IL@H-PP could promote the production of reactive oxygen species(ROS)by tumor-associated macrophages(TAMs),repolarizing them towards the M1 phenotype and remodeling the immunosuppressive microenvironment.In vitro/vivo results revealed the potential of PTT combined with SG inhibitors,which provides a new paradigm for antitumor and antimetastases.
基金This work was supported by the Key Project of National Natural Science Foundation of China under Grant No. 61332015 and the Natural Science Foundation of Shandong Province of China under Grant Nos. ZR2013FM302 and ZR2017MF057.
文摘With the rapid development of urban, the scale of the city is expanding day by day. The road environment is becoming more and more complicated. The vehicle ego-localization in complex road environment puts forward imperative requirements for intelligent driving technology. The reliable vehicle ego-localization, including the lane recognition and the vehicle position and attitude estimation, at the complex traffic intersection is significant for the intelligent driving of the vehicle. In this article, we focus on the complex road environment of the city, and propose a pose and position estimation method based on the road sign using only a monocular camera and a common GPS (global positioning system). Associated with the multi-sensor cascade system, this method can be a stable and reliable alternative when the precision of multi-sensor cascade system decreases. The experimental results show that, within 100 meters distance to the road signs, the pose error is less than 2 degrees, and the position error is less than one meter, which can reach the lane-level positioning accuracy. Through the comparison with the Beidou high-precision positioning system L202, our method is more accurate for detecting which lane the vehicle is driving on.
基金supported by the National Natural Science Foundation of China(Grant Nos.81870328,U2004203,81800267,82170281)Henan Thousand Talents Program(Grant No.ZYQR201912131)+2 种基金Henan Medical Science and Technology Joint Building Program(Grant No.2018020002)Henan Province Youth Talent Promoting Project(Grant No.2020HYTP051)Excellent Youth Science Foundation of Henan Province(Grant No.202300410362).
文摘Acute myocardial infarction(AMI)remains a dominant origin of morbidity,mortality and disability worldwide.Increases in reactive oxygen species(ROS)are key contributor to excessive cardiac injury after AMI.Here we developed an immobilized enzyme with Superoxide Dismutase(SOD)activity cross-link with Zr-based metal-organic framework(ZrMOF)(SOD-ZrMOF)for mitigate ROS-caused injury.In vitro and in vivo evidence indicates that SOD-ZrMOF exhibits excellent biocompatibility.By efficiently scavenging ROS and suppressing oxidative stress,SOD-ZrMOF can protect the function of mitochondria,reduce cell death and alleviate inflammation.More excitingly,long-term study using an animal model of AMI demonstrated that SOD-ZrMOF can reduce the infarct area,protect cardiac function,promote angiogenesis and inhibit pathological myocardial remodeling.Therefore,SOD-ZrMOF holds great potential as an efficacious and safe nanomaterial treatment for AMI.
文摘Erratum to Nano Research 2023,16(5):7600-7608 https://doi.org/10.1007/s12274-023-5382-y The name of the third author in original paper was unfortunately misspelled.It should be“Huimin Wang”,instead of“Huiming Wang”.
基金supported by the National Development and Reform Commission of China(No.20111158)。
文摘Acetylcholinesterase(AChE) is a key enzyme used to detect organophosphorus pesticide residues by the enzyme inhibition method.An accidental discovery of a mutant strain with AChE activity was made in our laboratory during the process of AChE expression by Pichia pastoris.The pPIC9 K-Drosophila melanogaster acetylcholinesterase(DmAChE)-like expression vector was constructed by codon optimization of this mutant strain,which was transformed into P.pastoris GS115,and positive clones were selected on yeast peptone dextrose(YPD) plate with G418 at 4.0 mg/mL.The GS115-pPIC9 K-DmAChE-like strain was subjected to 0.5% methanol induction expression for 120 h,with a protein band at 4.3 kDa found by the tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) pattern of the fermentation supernatant.After preliminary purification by ammonium sulfate precipitation,the enzyme activity was detected to be 76.9 U/(mL·min).In addition,the pesticide sensitivity test proved that DmAChE-like is selective and sensitive to organophosphorus pesticides.
基金This work is sponsored by the National Key Program for Developing Basic Sciences of China (No.2006CB400506) National Natural Science Foundation of China (No. 40675007)+2 种基金 Natural Science Foundation of Yunnan Province of China (2005D0006M) It is also supported by Research for Potential Effect of the 0zonosphere Change in the Regional Climate and Environment (CCSF2007-46)which is supported by Project for Climate Chang of Chinese Meteorological Administration, Project for Innovative Research Team of High Performance Computing in Yunnan University, and Collaboration Project between China Meteorological Administration and Yunnan University.
文摘Two common surface-dust emission schemes using critical wind speed and friction velocity were compared with the regional climate model RegCM3 in East Asia. In the comparison, transport of mineral dust and its distribution were simulated from March to April, 2001. Simulation results were also compared with TOMS aerosol index, showing that obvious differences exist in dust emission quantity and its column burden simulated by the dust emission schemes of friction velocity and wind speed criteria. The results obtained by the wind speed criterion are higher than that by friction velocity, bringing forth the problem whether or not the dust emission scheme matches the model. The obvious difference in the two schemes also explains the uncertainty of simulating mineral dust aerosol by modeling.
基金supported by the National Natural Science Foundation of China (21705036)the Natural Science Foundation of Hunan Province, China (2018JJ3035)the Fundamental Research Funds for the Central Universities from Hunan University
文摘1 Introduction Graphene is the first demonstration of a truly 2D atomic layer crystal, and has shown various excellent properties (e.g., ultrahigh carrier mobility, superior toughness, unique thermal conductivity, and good optics performance). The family of 2D crystals has grown to include metals (e.g., NbS2, VS2, TaS2), semiconductors (e.g., MoS2, WS2, ZrS2), and insulators (e.g., hexagonal boron nitride (hBN)), many of which are stable at ambient conditions [1]. 2D layered metal dichalcogenides (LMDs) materials [2].
基金sponsored by the National Key Program for Developing Basic Sciences of China(No.2006CB400506),KZCX2-YW-Q11-03the Hundred Talents Program(Aerosol Characteristics and its Climatic Impact)of the Chinese Academyof Sciences+2 种基金sponsored by the Chinese Natural Science Foundation(40975092)the Natural Science Foundation(2009CD021)the Foundation of Younger Scholars in Science and Technology(2009CI046)of Yunnan Province
文摘The results from some general circulation models show distinct radiative forcing (RF) by dust aerosol, which potentially has an effect on climate change. The direct RF and regional climatic effects of dust aerosol over the East Asian region are investigated in this study using NCAR's Community Atmospheric Model version 3.1. The negative RF at the top of the atmosphere (TOA) and the surface (SRF) has been revealed except for some high-albedo regions, which leads to a decrease in the surface air temperature and brings an increase of atmospheric radiative heating under both clear- and all-sky conditions. The decrease in the surface air temperature can be found over a wide region that includes the Indian peninsula and northwest China. It accompanies an increase in eastern China and the Korean peninsula, and temperature changes are not limited to regions with a large dust optical depth. A belt of vapor increase is revealed from the Indian peninsula extending east to northern China, while vapor content evidently deceases in southwest China. An increase in precipitation can also be found in the belt of increased vapor accompanying the reduction of precipitation across the regions to the south of 30~N. The pattern of rainfall change helps to offset the trend of increasing wetness in the south and increasing dryness in the north of China in recent years.
基金supported by the National Natural Science Foundation of China(No.11871160)the Fundamental Research Funds for the Central Universities of China(No.ZYGX2016J135)
文摘In the present paper, the rigidity of hypersurfaces in Euclidean space is revisited.The Darboux equation is highlighted and two new proofs of the rigidity are given via energy method and maximal principle, respectively.
基金supported by the Key Research and Development Program of Anhui Province,China(No.2022e07020048)the Clinical Research Incubation Program of The Second Hospital of Anhui Medical University,China(No.2020LCZD07)+2 种基金the National Natural Science Foundation of China(No.81872438)the Program of Research and Development of Key Common Technologies and Engineering of Major Scientific and Technological Achievements in Hefei,China(No.2021YL007)the Collaborative Innovation Program of Hefei Science Center CAS(China)(No.2022HSC-CIP015).
文摘The two most common inherited forms of colorectal cancer(CRC)are familial adenomatous polyposis and hereditary nonpolyposis colorectal cancer.They are caused by germline mutations in the APC and mismatch repair genes,respectively.1 The simultaneous inheritance of both an APC gene mutation and a mismatch repair gene mutation is very rare.In this study,we reported a coinheritance of mutations in the APC and MLH1 genes in a 20-year-old patient with CRC and multiple polyposes.We described for the first time the somatic mutational profile of a colorectal tumor harboring concurrent germline mutations in the APC and MLH1 genes.