Mercury is a threatening pollutant in food,herein,we developed a Tb^(3+)-nucleic acid probe-based label-free assay for mix-and-read,rapid detection of mercury pollution.The assay utilized the feature of light-up fluor...Mercury is a threatening pollutant in food,herein,we developed a Tb^(3+)-nucleic acid probe-based label-free assay for mix-and-read,rapid detection of mercury pollution.The assay utilized the feature of light-up fluorescence of terbium ions(Tb^(3+))via binding with single-strand DNA.Mercury ion,Hg^(2+)induced thymine(T)-rich DNA strand to form a double-strand structure(T-Hg^(2+)-T),thus leading to fluorescence reduction.Based on the principle,Hg^(2+)can be quantified based on the fluorescence of Tb^(3+),the limit of detection was 0.0689μmol/L and the linear range was 0.1-6.0μmol/L.Due to the specificity of T-Hg^(2+)-T artificial base pair,the assay could distinguish Hg^(2+)from other metal ions.The recovery rate was ranged in 98.71%-101.34%for detecting mercury pollution in three food samples.The assay is low-cost,separation-free and mix-to-read,thus was a competitive tool for detection of mercury pollution to ensure food safety.展开更多
Soybean(Glycine max) is a facultative short-day plant with a sensitive photoperiod perception and reaction system, which allows it to adjust its physiological state and gene regulatory networks to seasonal and diurnal...Soybean(Glycine max) is a facultative short-day plant with a sensitive photoperiod perception and reaction system, which allows it to adjust its physiological state and gene regulatory networks to seasonal and diurnal changes in environmental conditions. In the past few decades, soybean cultivation has spread from East Asia to areas throughout the world. Biologists and breeders must now confront the challenge of understanding the molecular mechanism of soybean photoperiodism and improving agronomic traits to enable this important crop to adapt to geographical and environmental changes. In this review, we summarize the genetic regulatory network underlying photoperiodic responses in soybean. Genomic and genetic studies have revealed that the circadian clock, in conjunction with the light perception pathways, regulates photoperiodic flowering. Here, we provide an annotated list of 844 candidate flowering genes in soybean, with their putative biological functions. Many photoperiod-related genes have been intensively selected during domestication and crop improvement. Finally, we describe recent progress in engineering photoperiod-responsive genes for improving agronomic traits to enhance geographic adaptation in soybean, as well as future prospects for research on soybean photoperiodic responses.展开更多
We developed a physical model to fundamentally understand the conductive filament(CF)formation and growth behavior in the switching layer during electroforming process in the metal-oxide-based resistive random-access ...We developed a physical model to fundamentally understand the conductive filament(CF)formation and growth behavior in the switching layer during electroforming process in the metal-oxide-based resistive random-access memories(RRAM).The effects of the electrode and oxide layer properties on the CF morphology evolution,current-voltage characteristic,local temperature,and electrical potential distribution have been systematically explored.It is found that choosing active electrodes with lower oxygen vacancy formation energy and oxides with small Lorenz number(ratio of thermal and electrical conductivity)enables CF formation at a smaller electroforming voltage and creates a CF with more homogeneous morphology.This work advances our understanding of the kinetic behaviors of the CF formation and growth during the electroforming process and could potentially guide the oxide and electrode materials selection to realize a more stable and functional RRAM.展开更多
The original version of this Article did not acknowledge Rama K.Vasudevan(vasudevanrk@ornl.gov)as a corresponding author.This has now been corrected in both the PDF and HTML versions of the Article.
Spatially resolved time and voltage-dependent polarization dynamics in PbTiO3 thin films is explored using dynamic piezoresponse force microscopy(D-PFM)in conjunction with interferometric displacement sensing.This app...Spatially resolved time and voltage-dependent polarization dynamics in PbTiO3 thin films is explored using dynamic piezoresponse force microscopy(D-PFM)in conjunction with interferometric displacement sensing.This approach gives rise to 4D data sets containing information on bias-dependent relaxation dynamics at each spatial location without long-range electrostatic artifacts.To interpret these data sets in the absence of defined physical models,we employ a non-negative tensor factorization method which clearly presents the data as a product of simple behaviors allowing for direct physics interpretation.Correspondingly,we perform phase-field modeling finding the existence of‘hard’and‘soft’domain wall edges.This approach can be extended to other multidimensional spectroscopies for which even exploratory data analysis leads to unsatisfactory results due to many components in the decomposition.展开更多
基金financially supported by National Natural Science Foundation of China(22074100)the Young Elite Scientist Sponsorship Program by CAST(YESS20200036)+3 种基金the Researchers Supporting Project Number RSP-2021/138King Saud University,Riyadh,Saudi ArabiaTechnological Innovation R&D Project of Chengdu City(2019-YF05-31702266-SN)Sichuan University-Panzhihua City joint Project(2020CDPZH-5)。
文摘Mercury is a threatening pollutant in food,herein,we developed a Tb^(3+)-nucleic acid probe-based label-free assay for mix-and-read,rapid detection of mercury pollution.The assay utilized the feature of light-up fluorescence of terbium ions(Tb^(3+))via binding with single-strand DNA.Mercury ion,Hg^(2+)induced thymine(T)-rich DNA strand to form a double-strand structure(T-Hg^(2+)-T),thus leading to fluorescence reduction.Based on the principle,Hg^(2+)can be quantified based on the fluorescence of Tb^(3+),the limit of detection was 0.0689μmol/L and the linear range was 0.1-6.0μmol/L.Due to the specificity of T-Hg^(2+)-T artificial base pair,the assay could distinguish Hg^(2+)from other metal ions.The recovery rate was ranged in 98.71%-101.34%for detecting mercury pollution in three food samples.The assay is low-cost,separation-free and mix-to-read,thus was a competitive tool for detection of mercury pollution to ensure food safety.
基金supported by the National Key Research and Development Plan(2016YFD0101005)National Natural Science Foundation of China(31422041)+1 种基金China Postdoctoral Science Foundation Grant(2014M56013)the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences
文摘Soybean(Glycine max) is a facultative short-day plant with a sensitive photoperiod perception and reaction system, which allows it to adjust its physiological state and gene regulatory networks to seasonal and diurnal changes in environmental conditions. In the past few decades, soybean cultivation has spread from East Asia to areas throughout the world. Biologists and breeders must now confront the challenge of understanding the molecular mechanism of soybean photoperiodism and improving agronomic traits to enable this important crop to adapt to geographical and environmental changes. In this review, we summarize the genetic regulatory network underlying photoperiodic responses in soybean. Genomic and genetic studies have revealed that the circadian clock, in conjunction with the light perception pathways, regulates photoperiodic flowering. Here, we provide an annotated list of 844 candidate flowering genes in soybean, with their putative biological functions. Many photoperiod-related genes have been intensively selected during domestication and crop improvement. Finally, we describe recent progress in engineering photoperiod-responsive genes for improving agronomic traits to enhance geographic adaptation in soybean, as well as future prospects for research on soybean photoperiodic responses.
基金K.Z.and Y.C.acknowledge the support from the National Science Foundation(NSF)under the award number NSF 2132105the Texas Advanced Computing Center(TACC)at The University of Texas at Austin for providing HPC resources that have contributed to the research results reported within this paper(http://www.tacc.utexas.edu)+1 种基金Y.C.also acknowledges the startup funding from the University of Texas at ArlingtonP.G.(physical model,analysis and interpretation)was supported by the Center for Nanophase Materials Sciences,which is a DOE Office of Science User Facility.
文摘We developed a physical model to fundamentally understand the conductive filament(CF)formation and growth behavior in the switching layer during electroforming process in the metal-oxide-based resistive random-access memories(RRAM).The effects of the electrode and oxide layer properties on the CF morphology evolution,current-voltage characteristic,local temperature,and electrical potential distribution have been systematically explored.It is found that choosing active electrodes with lower oxygen vacancy formation energy and oxides with small Lorenz number(ratio of thermal and electrical conductivity)enables CF formation at a smaller electroforming voltage and creates a CF with more homogeneous morphology.This work advances our understanding of the kinetic behaviors of the CF formation and growth during the electroforming process and could potentially guide the oxide and electrode materials selection to realize a more stable and functional RRAM.
文摘The original version of this Article did not acknowledge Rama K.Vasudevan(vasudevanrk@ornl.gov)as a corresponding author.This has now been corrected in both the PDF and HTML versions of the Article.
基金This research used resources of the Compute and Data Environment for Science(CADES)at the Oak Ridge National Laboratory,which is supported by the Office of Science of the U.S Department of Energy under Contract No.DE-AC05-00OR22725This work was partially supported by the JSPSKAKENHI Grant Nos.15H04121,and 26220907(H.F.).
文摘Spatially resolved time and voltage-dependent polarization dynamics in PbTiO3 thin films is explored using dynamic piezoresponse force microscopy(D-PFM)in conjunction with interferometric displacement sensing.This approach gives rise to 4D data sets containing information on bias-dependent relaxation dynamics at each spatial location without long-range electrostatic artifacts.To interpret these data sets in the absence of defined physical models,we employ a non-negative tensor factorization method which clearly presents the data as a product of simple behaviors allowing for direct physics interpretation.Correspondingly,we perform phase-field modeling finding the existence of‘hard’and‘soft’domain wall edges.This approach can be extended to other multidimensional spectroscopies for which even exploratory data analysis leads to unsatisfactory results due to many components in the decomposition.
