It is known that the high level of sugar including glucose suppresses seed germination through ABA signal.ABI5 is an essential component to mediate ABA-dependent seed germination inhibition,but underlying mechanism ne...It is known that the high level of sugar including glucose suppresses seed germination through ABA signal.ABI5 is an essential component to mediate ABA-dependent seed germination inhibition,but underlying mechanism needs more investigation.Previous study demonstrated the PIF4 activated the expression of ABI5 to suppress seed germination in darkness.Here we reported that PIF4 also mediated the seed germination inhibition through ABI5 under high concentration of glucose treatment.Furthermore,we found that PIF4 interacted with PAP1,the central factor to control anthocyanin biosynthesis.Such interaction was confirmed in vitro and in planta.Biochemical and physiological analysis revealed that PAP1 bond the promoter of ABI5 to suppress its expression,thus enhanced seed germination under high concentration of glucose treatment.Specially,PAP1 competed with PIF4 to antagonize the activation of PIF4 on ABI5 expression,thus promoted seed germination under high glucose treatment.Given these,we uncover a novel role for PIF4 and PAP1 in controlling seed germination under high glucose treatment,and reveal their antagonistic mechanism by which coordinates ABI5 expression to control seed germination in response to the glucose signal.展开更多
Putrescine(Put)as the compound of plant polyamines is catalyzed by arginine decarboxylase(ADC),which is encoded by two members,ADC1 and ADC2 in Arabidopsis,and ADC2 is mainly responsible for Put biosynthesis.Accumulat...Putrescine(Put)as the compound of plant polyamines is catalyzed by arginine decarboxylase(ADC),which is encoded by two members,ADC1 and ADC2 in Arabidopsis,and ADC2 is mainly responsible for Put biosynthesis.Accumulated evidence demonstrates the important function of Put in plant growth and development,but its role in regulating seed germination under high temperature(HT)has not been reported yet.SOMNUS(SOM)is the negative regulator for seed germination thermoinhibition by altering downstream gibberellin(GA)and abscisic acid(ABA)metabolism.In this study,we found exogenous application of Put obviously alleviated the inhibition effect of HT on seed germination.Whereas pharmacological inhibition of endogenous Put level reduced seed germination under HT.Consistently,HT induced the rapid accumulation of Put level,and the adc2 mutant defi-ciency in Put biosynthesis also showed more sensitivity to HT stress.Furthermore,we found that the Put signal suppressed the expression of SOM and changed the transcriptional patterns of genes associated with GA/ABA metabolism.Genetic analysis also revealed SOM was epistatic to ADC2 to alter GA/ABA metabolism.Collectively,our finding reveals the novel function of Put in controlling seed germination under HT through SOM,and provides the possibility to develop Put as the innovational regulator for uniform seed germination under HT stress.展开更多
Plant branching development plays an important role in plant morphogenesis(aboveground plant type),the number and angle of branches are important agronomic characters that determine crop plant type.Effective branches ...Plant branching development plays an important role in plant morphogenesis(aboveground plant type),the number and angle of branches are important agronomic characters that determine crop plant type.Effective branches determine the number of panicles or pods of crops and then control the yield of crops.With the rapid development of plant genomics and molecular genetics,great progress has been made in the study of branching development.In recent years,a series of important branching-related genes have been validated from Arabidopsis thaliana,rice,pea,tomato and maize mutants.It is reviewed that plant branching development is controlled by genetic elements and plant hormones,such as auxin,cytokinin and lactones(or lactone derivatives),as well as by environment and genetic elements.Meanwhile,shoot architecture in crop breeding was discussed in order to provide theoretical basis for the study of crop branching regulation.展开更多
[Objectives]To explore the pharmacodynamic material basis of Sijicao granules for the treatment of eczema through chemical composition-network pharmacology.[Methods]First of all,the chemical constituents of Polygonum ...[Objectives]To explore the pharmacodynamic material basis of Sijicao granules for the treatment of eczema through chemical composition-network pharmacology.[Methods]First of all,the chemical constituents of Polygonum capitatum and Plantago asiatica from Sijicao granules were collected,and the relevant target information of the constituents was collected by TCMSP,PubChem,DisGeNET,GeneCards and STRING databases.Furthermore,Cytoscape 3.8.2 software was used to construct the chemical compounds-target network map of Sijicao granules.Finally,STRING database was used for PPI protein network analysis,GO functional enrichment analysis and KEGG pathway enrichment analysis of core targets,and molecular docking between core constituents and protein targets was also performed.[Results]30 constituents,including quercetin,kaempferol,luteolin,ellagic acid and gallic acid,were discovered to be the key effective compounds of Sijicao granules in the treatment of eczema.And its core action protein targets were PTGS2,NOS2,AKT1,TP53,IL6,HMOX1.What s more,through GO functional enrichment analysis of biological process(BP),cell component(CC),molecular function(MF)analysis and KEGG pathway enrichment analysis,the main pathways of action of Sijicao granules for the treatment of eczema including IL-17 signaling pathway,T cell receptor signaling pathway,cancer signaling pathway,TNF signaling pathway and Relaxin signaling pathway.In addition,molecular docking results displayed that the primary active constituents quercetin,kaempferol and luteolin were well combined with the core protein targets AKT1 and IL6.[Conclusions]Sijicao granules could play an important role for the treatment of eczema through multi-component,multi-target,multi-pathway and their interaction.展开更多
The designed Ce^3+-doped alkaline-earth silicate phosphors CamSr2 m-nBanSiO4:Ce^3+,Li^+ (CSBS:Ce^3+) were synthesized by a high temperature solid-state reaction. The crystal field splitting and the centroid sh...The designed Ce^3+-doped alkaline-earth silicate phosphors CamSr2 m-nBanSiO4:Ce^3+,Li^+ (CSBS:Ce^3+) were synthesized by a high temperature solid-state reaction. The crystal field splitting and the centroid shift from the flee ion energy of 5d configuration were approximated from the spectrum for Ca2SiO4, Sr2SiO4 and Ba2SiO4 phosphors. The single-phase purity was checked by means of X-ray diffraction. Here, when the doped concentration of Ca2. is less than 80% (m 〈 1.6), we report the structural phase transformation from monoclinic system [3-Ca2Si04 to orthorhombic system α′-Ca2SiO4. The phosphors excited by near-ultraviolet (NUV) light at wavelengths ranging from 200 to 400 nm demonstrate a broad asymmetric blue emission band. The emission peak wavelength redshifts firstly from 417 nm of Ca2SiO4 to 438 nm of Sro.3Cal.6SiO4, and then blueshifts to 411 nm of Sr2SiO4, and the end of 401 nm of Ba2SiOa. These results indicate that the tunable blue-emission of the phosphors can he realized through changing the solid solution components, which has a potential use as a blue component for fabricated precision modulation LEDs light sources and auxiliaries of SSC plastics films for different plant growths. We discuss in detail the possible mechanism and energy diagram of the tunable blue luminescence in CamSr2-M-nBaNSiO4:Ce^3+.Li^+ phosphors.展开更多
基金funded by the National Natural Science Foundation of China(Grant No.31970289).
文摘It is known that the high level of sugar including glucose suppresses seed germination through ABA signal.ABI5 is an essential component to mediate ABA-dependent seed germination inhibition,but underlying mechanism needs more investigation.Previous study demonstrated the PIF4 activated the expression of ABI5 to suppress seed germination in darkness.Here we reported that PIF4 also mediated the seed germination inhibition through ABI5 under high concentration of glucose treatment.Furthermore,we found that PIF4 interacted with PAP1,the central factor to control anthocyanin biosynthesis.Such interaction was confirmed in vitro and in planta.Biochemical and physiological analysis revealed that PAP1 bond the promoter of ABI5 to suppress its expression,thus enhanced seed germination under high concentration of glucose treatment.Specially,PAP1 competed with PIF4 to antagonize the activation of PIF4 on ABI5 expression,thus promoted seed germination under high glucose treatment.Given these,we uncover a novel role for PIF4 and PAP1 in controlling seed germination under high glucose treatment,and reveal their antagonistic mechanism by which coordinates ABI5 expression to control seed germination in response to the glucose signal.
基金the National Natural Science Foundation of China(Grant No.32170562).
文摘Putrescine(Put)as the compound of plant polyamines is catalyzed by arginine decarboxylase(ADC),which is encoded by two members,ADC1 and ADC2 in Arabidopsis,and ADC2 is mainly responsible for Put biosynthesis.Accumulated evidence demonstrates the important function of Put in plant growth and development,but its role in regulating seed germination under high temperature(HT)has not been reported yet.SOMNUS(SOM)is the negative regulator for seed germination thermoinhibition by altering downstream gibberellin(GA)and abscisic acid(ABA)metabolism.In this study,we found exogenous application of Put obviously alleviated the inhibition effect of HT on seed germination.Whereas pharmacological inhibition of endogenous Put level reduced seed germination under HT.Consistently,HT induced the rapid accumulation of Put level,and the adc2 mutant defi-ciency in Put biosynthesis also showed more sensitivity to HT stress.Furthermore,we found that the Put signal suppressed the expression of SOM and changed the transcriptional patterns of genes associated with GA/ABA metabolism.Genetic analysis also revealed SOM was epistatic to ADC2 to alter GA/ABA metabolism.Collectively,our finding reveals the novel function of Put in controlling seed germination under HT through SOM,and provides the possibility to develop Put as the innovational regulator for uniform seed germination under HT stress.
文摘Plant branching development plays an important role in plant morphogenesis(aboveground plant type),the number and angle of branches are important agronomic characters that determine crop plant type.Effective branches determine the number of panicles or pods of crops and then control the yield of crops.With the rapid development of plant genomics and molecular genetics,great progress has been made in the study of branching development.In recent years,a series of important branching-related genes have been validated from Arabidopsis thaliana,rice,pea,tomato and maize mutants.It is reviewed that plant branching development is controlled by genetic elements and plant hormones,such as auxin,cytokinin and lactones(or lactone derivatives),as well as by environment and genetic elements.Meanwhile,shoot architecture in crop breeding was discussed in order to provide theoretical basis for the study of crop branching regulation.
基金Supported by Science and Technology Planning Project of Guizhou Province[QKHJC-ZK(2022)362]Science and Technology Achievements Transfer and Transformation Project of Guizhou Provincial Department of Education[(2022)064]+1 种基金Higher Education Institution Engineering Research Center of Guizhou Provincial Department of Education[(2023)035,No.(2023)zk01]National Undergraduate Innovation Training Project(202210660131,202310660082).
文摘[Objectives]To explore the pharmacodynamic material basis of Sijicao granules for the treatment of eczema through chemical composition-network pharmacology.[Methods]First of all,the chemical constituents of Polygonum capitatum and Plantago asiatica from Sijicao granules were collected,and the relevant target information of the constituents was collected by TCMSP,PubChem,DisGeNET,GeneCards and STRING databases.Furthermore,Cytoscape 3.8.2 software was used to construct the chemical compounds-target network map of Sijicao granules.Finally,STRING database was used for PPI protein network analysis,GO functional enrichment analysis and KEGG pathway enrichment analysis of core targets,and molecular docking between core constituents and protein targets was also performed.[Results]30 constituents,including quercetin,kaempferol,luteolin,ellagic acid and gallic acid,were discovered to be the key effective compounds of Sijicao granules in the treatment of eczema.And its core action protein targets were PTGS2,NOS2,AKT1,TP53,IL6,HMOX1.What s more,through GO functional enrichment analysis of biological process(BP),cell component(CC),molecular function(MF)analysis and KEGG pathway enrichment analysis,the main pathways of action of Sijicao granules for the treatment of eczema including IL-17 signaling pathway,T cell receptor signaling pathway,cancer signaling pathway,TNF signaling pathway and Relaxin signaling pathway.In addition,molecular docking results displayed that the primary active constituents quercetin,kaempferol and luteolin were well combined with the core protein targets AKT1 and IL6.[Conclusions]Sijicao granules could play an important role for the treatment of eczema through multi-component,multi-target,multi-pathway and their interaction.
基金Project supported by National Key Research and Development Program(2016YFB0302403)the National Natural Science Foundation of China(21571059)+1 种基金Natural Science Foundation of Hunan Province(2015JJ2100)Hunan Provincial Innovation Foundation for Postgraduate(CX2017B180)
文摘The designed Ce^3+-doped alkaline-earth silicate phosphors CamSr2 m-nBanSiO4:Ce^3+,Li^+ (CSBS:Ce^3+) were synthesized by a high temperature solid-state reaction. The crystal field splitting and the centroid shift from the flee ion energy of 5d configuration were approximated from the spectrum for Ca2SiO4, Sr2SiO4 and Ba2SiO4 phosphors. The single-phase purity was checked by means of X-ray diffraction. Here, when the doped concentration of Ca2. is less than 80% (m 〈 1.6), we report the structural phase transformation from monoclinic system [3-Ca2Si04 to orthorhombic system α′-Ca2SiO4. The phosphors excited by near-ultraviolet (NUV) light at wavelengths ranging from 200 to 400 nm demonstrate a broad asymmetric blue emission band. The emission peak wavelength redshifts firstly from 417 nm of Ca2SiO4 to 438 nm of Sro.3Cal.6SiO4, and then blueshifts to 411 nm of Sr2SiO4, and the end of 401 nm of Ba2SiOa. These results indicate that the tunable blue-emission of the phosphors can he realized through changing the solid solution components, which has a potential use as a blue component for fabricated precision modulation LEDs light sources and auxiliaries of SSC plastics films for different plant growths. We discuss in detail the possible mechanism and energy diagram of the tunable blue luminescence in CamSr2-M-nBaNSiO4:Ce^3+.Li^+ phosphors.