V4 is a Gram-negative,plant growth promoting endophytic bacterium that promotes the growth of tea plants.The appearance of V4 is rod shaped,with average dimensions of 1.34−1.5×0.32−0.39μm and flagellum at both e...V4 is a Gram-negative,plant growth promoting endophytic bacterium that promotes the growth of tea plants.The appearance of V4 is rod shaped,with average dimensions of 1.34−1.5×0.32−0.39μm and flagellum at both ends.The complete genome contains one circular chromosome and two plasmids.It is 4,697,109 bp in size,and contains 4,189 protein-coding genes,four gene islands and two prophages.Taxonomic classification suggested that V4 was a strain of Erwinia aphidicola.It was possible to find genes involved in plant growth promotion traits present in the genome of V4.Meanwhile,V4 was consistent with plant growth-promoting endophytic bacteria containing key synthetic genes associated with IAA synthesis,and P-solubilization,siderophores.V4 has siderophore biosynthesis genes compared with plant pathogenic bacteria showing stronger survival ability and the ability to interaction with the host plant.In addition,V4 endophytic bacteria possess a higher copy number of genes for flagellar assembly,bacterial chemotaxis and P-pilus assembly indicating stronger colonization and communication ability with host plants compared with five other bacteria in comparative genomic analysis.Analysis of the V4 endophytic bacterium complete genome sequence provides novel insights into the endophytic bacteria-host plant relationship,and suggests many candidate genes for post-genomic experiments.展开更多
基金supported by the Natural Science Foundation of Anhui Province,Outstanding Youth Project(2008085J18)National Natural Science Foundation of China(NSFC,Grant No.31870679).
文摘V4 is a Gram-negative,plant growth promoting endophytic bacterium that promotes the growth of tea plants.The appearance of V4 is rod shaped,with average dimensions of 1.34−1.5×0.32−0.39μm and flagellum at both ends.The complete genome contains one circular chromosome and two plasmids.It is 4,697,109 bp in size,and contains 4,189 protein-coding genes,four gene islands and two prophages.Taxonomic classification suggested that V4 was a strain of Erwinia aphidicola.It was possible to find genes involved in plant growth promotion traits present in the genome of V4.Meanwhile,V4 was consistent with plant growth-promoting endophytic bacteria containing key synthetic genes associated with IAA synthesis,and P-solubilization,siderophores.V4 has siderophore biosynthesis genes compared with plant pathogenic bacteria showing stronger survival ability and the ability to interaction with the host plant.In addition,V4 endophytic bacteria possess a higher copy number of genes for flagellar assembly,bacterial chemotaxis and P-pilus assembly indicating stronger colonization and communication ability with host plants compared with five other bacteria in comparative genomic analysis.Analysis of the V4 endophytic bacterium complete genome sequence provides novel insights into the endophytic bacteria-host plant relationship,and suggests many candidate genes for post-genomic experiments.
