[Objective] This study aimed to investigate the effect and mechanism of miRNA-139-Sp in the occurrence and development of colorectal cancer. [ Method] Forty SPF grade C57BL/6 male mice, including 20 miRNA-139-Sp knock...[Objective] This study aimed to investigate the effect and mechanism of miRNA-139-Sp in the occurrence and development of colorectal cancer. [ Method] Forty SPF grade C57BL/6 male mice, including 20 miRNA-139-Sp knockout （KO） mice and 20 wild-type （WT） mice, were selected to establish the animal models of colorectal cancer. The occurrence and development of colorectal cancer in mice were observed. H＆E staining was performed to analyze histopatho- logical changes and immunohistochemical expression of proliferative proteins. The level of apoptosis of tumor cells was detected by TUNEL assay. [ Result] During cancer induction, the body weight of mice in miR-139-Sp KO group declined more significantly and recovered slower with lower survival rate compared with that in WT group. The colons of mice in miR-139-Sp KO group were shorter with more tumors than those in WT group. In addition, mice in KO group exhibited rapider progression of colorectal cancer, higher deterioration degree and lower apoptosis level of tumor cells. [ Conclusion] miRNA-139-Sp plays an important role in the occurrence and development of colorectal cancer, which can not only inhibit tumor degeneration and proliferation of tumor cells, but also promote the apoptosis of tumor cells.展开更多
基金Supported by Fund of Science and Technology Department of Zhejiang Province(2015C37086)
文摘[Objective] This study aimed to investigate the effect and mechanism of miRNA-139-Sp in the occurrence and development of colorectal cancer. [ Method] Forty SPF grade C57BL/6 male mice, including 20 miRNA-139-Sp knockout （KO） mice and 20 wild-type （WT） mice, were selected to establish the animal models of colorectal cancer. The occurrence and development of colorectal cancer in mice were observed. H＆E staining was performed to analyze histopatho- logical changes and immunohistochemical expression of proliferative proteins. The level of apoptosis of tumor cells was detected by TUNEL assay. [ Result] During cancer induction, the body weight of mice in miR-139-Sp KO group declined more significantly and recovered slower with lower survival rate compared with that in WT group. The colons of mice in miR-139-Sp KO group were shorter with more tumors than those in WT group. In addition, mice in KO group exhibited rapider progression of colorectal cancer, higher deterioration degree and lower apoptosis level of tumor cells. [ Conclusion] miRNA-139-Sp plays an important role in the occurrence and development of colorectal cancer, which can not only inhibit tumor degeneration and proliferation of tumor cells, but also promote the apoptosis of tumor cells.
