Dry environment on the expression of lacrimal gland S100A9,Anxa1,and Clu in rats via proteomics

●AIM:To investigate the underlying mechanism of dry environment(autumn dryness)affecting the lacrimal glands in rats.●METHODS:Twenty Sprague-Dawley rats were randomly divided into two groups.The rats were fed in specific pathogen free environment as the control group(n=10),and the rats fed in dry environment as the dryness group(n=10).After 24d,lacrimal glands were collected from the rats.The tissues morphology was observed by hematoxylineosin(HE)staining.Tandem mass tags(TMT)quantitative proteomics analysis technology was used to screen the differential expressed proteins of lacrimal glands between the two groups,then bioinformatics analysis was performed.Further,the immunohistochemical(IHC)method was used to verify the target proteins.●RESULTS:In dryness group,the lacrimal glands lobule atrophied,the glandular cavities enlarged,the sparse nuclear distribution and scattered inflammatory infiltration between the acinus were observed.The proteomics exhibited that a total of 195 up-regulated and 236 downregulated differential expressed proteins screened from the lacrimal glands of rats.It was indicated that the biological processes(BP)of differential expressed proteins mainly included cell processes and single BP.The cellular compositions of differential expressed proteins mainly located in cells,organelles.The molecular functions of differential expressed proteins mainly included binding,catalytic activity.Moreover,the Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis showed that the differential expressed proteins mainly involved lysosome,complement and coagulation cascade,and ribosome pathway.The IHC result verified that the up-regulated expression proteins of Protein S100A9(S100A9),Annexin A1(Anxa1),and Clusterin(Clu)in lacrimal glands of rats in dryness group were higher than control group.●CONCLUSION:The up-regulated expression proteins of S100A9,Anxa1,and Clu may be the potential mechanisms of dry eye symptoms caused by dry environment.This study provides clues of dry environments causing eye-related diseases for further studies.

Short-term Preoperative Octreotide for Thyrotropin-secreting Pituitary Adenoma

Background： Thyrotropin-secreting pituitary adenomas （TSHomas） are a rare cause of hyperthyroidism. Somatostatin （SST） analogs work by interacting with somatostatin receptors （SSTRs）. This study aimed to evaluate short-term preoperative octreotide （OCT） use in TSHoma patients and to investigate SSTR2 and SSTR5 expression and observe structural changes in tumor tissue. Methods： We reviewed records and samples from eight TSHoma patients treated between July 2012 and July 2015. We tested immunohistochemically for SSTR2/5 expression and examined TSHoma cells for morphological changes. Signed rank sum test was used to compare the efficacy of short-term preoperative OCT treatment. Results： OCT treatment （median time： 7.9 days, range： 3-16 days; median total dose： 1.8 mg, range： 0.94.2 mg） led to significant decrease in all patients＇ thyroid hormone levels （FT3 [nmol/L]： 8.33 [7.02, 12.29] to 4.67 [3.52, 5.37] [P = 0.008]; FT4 [pmol/L]： 25.36 [21.34, 28.99] to 16.66 [14.88, 21.49] [P = 0.016]; and TSH [gU/ml]： 5.80 [4.37, 6.78] to 0.57 [0.19, 1.24] [P = 0.008]）. All the eight tumor specimens expressed high SSTR2 protein levels; 5/8 expressed high SSTRS, but 3/8 that expressed low SSTR5 presented a significantly higher TS H suppression rate （P = 0.036）. Electron microscopy showed subcellular level impairments, including clumped nuclear chromatin and reduced cytoplasmic volume. Golgi complexes were observed in the OCT-treated TSHoma specimens. Conclusions： OCT can control hormone levels and damage the ultrastructure of tumor cells and organelles. Short-term response to OCT may be related to SSTR5 expression. Preoperative SST analog treatment for TSHoma could be considered as a combination therapy.

多元化的多样性:连续6周交通日志的购物出行分析(英文)

目的:探究个人的购物出行模式随时间的变化差异性。探讨个人在星期中的天变化,量化多样化的特征和识别个体间的异质性。创新点:1.使用连续6周的数据进行动态交通行为分析;2.建立二元逻辑回归模型,识别个体间购物出行模式的异质性。方法:1.通过描述性统计分析,解析不同群体之间的购物行为的差异、每周购物天数的分布以及每周每人购物天数的标准偏差(表1、图1和2);2.通过回归模型分析,得出解释变量对购物地点和频率的影响结论(表2和3);3.构建二元逻辑回归模型,识别个体间购物出行模式的异质性,找出具有常规购物行为的群体(表4)。结论:1.低收入和卡尔斯鲁厄居住地对日常购物目的地的多样性呈正影响关系,而全职工作和男性对日常购物目的地的多样性呈负影响关系;2.连续6周的日常购物出行次数受到35~44岁的年龄范围、2500~2999德国马克的家庭月收入以及市中心居住地区的积极影响;3.购物地点和频率的多样性是随着个人属性和居住地点以系统的方式变化。

Diagnostic Significance of Intraoperative Ultrasound Contrast in Evaluating the Resection Degree of Brain Glioma By Transmission Electron Microscopic Examination

Background：Contrast-enhanced ultrasound is a dynamic and continuous modality providing real-time view of vascularization and flow distribution patterns of different organs and tumors.In order to evaluate the diagnostic significance of intraoperative contrast-enhanced ultrasound in assessing the resection degree of brain glioma by transmission electron microscopic （TEM） examination,it is important to have specific knowledge about contrast-enhanced ultrasound.Methods：Ultrasound contrast was applied in operations of 120 cases of brain glioma,to evaluate the degree of tumor resection.Biopsy tissues were obtained the suspicious residual tumors surrounding the tumor cavity.The sensitivity and specificity of the residual tumors were determined by the intraoperative ultrasound contrast according to TEM examination results.Results：There were 44 cases of low-grade gliomas and 76 cases of high-grade gliomas.Three hundred and sixty biopsy tissues were obtained.The sensitivity of intraoperative ultrasound contrast in diagnosing the residual tumor was 62.2%,while the specificity degree of it was 92.8%.The consistency coefficient of the ultrasound contrast diagnosis and TEM examination results was 0.584 （Kappa =0.584）,which was between 0.4 and 0.6,therefore it was of medium consistency.Conclusions：Intraoperative ultrasound contrast was of a high sensitivity and specificity in evaluating the excision degree of tumor.The consistency of the residual tumor rate detected,respectively,by ultrasound contrast and TEM examination was of medium consistency.The application of intraoperative ultrasound contrast can improve the resection rate of brain glioma.

日本大阪都市圈居民的年龄、居住位置与家庭生命周期对小汽车使用与保有的影响（英文）

研究目的:探究城市居民小汽车交通行为变化的原因有多少来自人口统计特性的变化,有多少来自城市结构的改变。创新要点:考虑家庭成员的生活任务分工以及家庭资源的分配等对每个家庭成员出行方式的影响,构建基于家庭生命周期的小汽车出行行为模型,为控制小汽车出行对策的研究提供准确依据。研究方法:采用调查与统计理论,掌握居民的出行行为特征;基于经济计量学建模方法,构建居民小汽车出行行为联立方程模型。重要结论:城市结构关系的改变在抵消了人口统计特性的变化对小汽车交通产生的负面影响之后,小汽车交通总体呈上升趋势。