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Effects of Abscisic Acid on Cold Resistance in Digitaria sanguinalis(L.)Scop.
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作者 Rongbo ZHOU yihong hu +3 位作者 Li ZHOU Jie PENG Fan XIE Chenzhong JIN 《Agricultural Biotechnology》 2024年第1期15-16,19,共3页
[Objectives]This study was conducted to detect the protective effect of abscisic acid on chilling injury of Digitaria sanguinalis(L.)Scop,and whether this effect is related to antioxidant enzymes and osmotic adjustmen... [Objectives]This study was conducted to detect the protective effect of abscisic acid on chilling injury of Digitaria sanguinalis(L.)Scop,and whether this effect is related to antioxidant enzymes and osmotic adjustment.[Methods]D.sanguinalis plants were sprayed with abscisic acid solution,and exposed to cold stress at 15℃for 3 d after one day and then at 5℃for 25 to 30 d in a growth chamber.The changes of plant osmotic potential under this treatment were detected.[Results]Under low temperature stress,the osmotic potential of plants in the abscisic acid treatment and the control increased,but the osmotic potential level of the abscisic acid treatment plants was lower.The SOD activity of plants in the ABA treatment and the control decreased under low temperature stress.Under low temperature stress,the activity of catalase and peroxidase in ABA-treated plants was higher than that in control plants.[Conclusions]This study provides a theoretical basis for the impact of abscisic acid on the physiological response of D.sanguinalis to cold injury. 展开更多
关键词 Digitaria sanguinalis Abscisic acid CAT POD ROS SOD
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Study on Antioxidant Enzyme Activity and Physiology and Biochemistry of Solanum nigrum L. under Glyphosate Stress
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作者 Si LIU Tingting ZOU +3 位作者 Wenshuai ZENG Zihan MEI Chenzhong JIN yihong hu 《Agricultural Biotechnology》 2024年第2期1-4,7,共5页
[Objectives] This study was conducted to investigate the scientific prevention and control of Solanum nigrum L. [Methods] Through experiments on S. nigrum from different sources, it was found that glyphosate stress ha... [Objectives] This study was conducted to investigate the scientific prevention and control of Solanum nigrum L. [Methods] Through experiments on S. nigrum from different sources, it was found that glyphosate stress had significant effects on antioxidant enzyme activity and oxidative damage of sensitive S. nigrum plants. [Results] Sensitive S. nigrum showed oxidative damage under glyphosate stress, while resistant S. nigrum responded to adversity damage by improving its antioxidant enzyme activity. The experimental results showed that the antioxidant enzymes and reduced glutathione of S. nigrum had certain metabolic detoxification effects under glyphosate stress. [Conclusions] This study provides a theoretical basis for scientific prevention and control of S. nigrum , and has a certain reference value for revealing the glyphosate resistance mechanism of S. nigrum . 展开更多
关键词 GLYPHOSATE Solanum nigrum L. Antioxidant enzyme MALONDIALDEHYDE GLUTATHIONE
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Polysaccharide extract from Rosa laevigata fruit attenuates inflammatory obesity by targeting redox balance and gut interface in high-fat diet-fed rats 被引量:2
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作者 Xuejiao Zhang Chenzhong Jin +4 位作者 Han Liu yihong hu Yunyun Zhou Weiguo Wu Si Qin 《Food Science and Human Wellness》 SCIE CSCD 2023年第2期442-453,共12页
Low-molecular-weight polysaccharides(RLPs)extracted from Rosa laevigata fruits showed multiple biofunctions in Asia.This study aimed to investigate its anti-inflammatory obesity effect in high-fat dietfed rats and fur... Low-molecular-weight polysaccharides(RLPs)extracted from Rosa laevigata fruits showed multiple biofunctions in Asia.This study aimed to investigate its anti-inflammatory obesity effect in high-fat dietfed rats and further elucidated the underlying molecular mechanism by multi-omics methods.The results showed that RLPs administration had significantly restored immune organ indexes and reduced body weight gain.RNA-seq revealed that the effect of RLPs was partially attributed to its regulation on PPARs signaling by increasing the expressions of Scd,Acox3 and Hmgcs2,and on other redox-related pathways by decreasing the expressions of Cyp2 e1,Il1-r1 and Lbp.Moreover,16 S rRNA sequencing coupled with metagenome sequencing showed that RLPs had significantly reduced the ratio of Firmicutes/Bacteroidetes from 8.01 to2.37,and significantly increased the relative abundances of Alistipes,Prevotella,and Akkermansia from 0.36%,1.10%and 2.61%to 0.65%,2.37%and 4.42%,respectively.Spearman correlation analysis result indicated that the abundances of Lachnospiraceae,Prevotella and Bacteroidales were significantly negatively correlated with obesity phenotype,liver function and inflammatory factors.These results revealed that RLPs exerted significant anti-inflammatory obesity property partially via regulation on gut microbiota interface and the redox balance.Therefore,RLPs could be a promising functional food resource with the potential for redox imb alance-related diseases chemoprevention. 展开更多
关键词 Rosa laevigata POLYSACCHARIDES Multi-omics Redox Gut microbiota
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Effects of Different Seed Coating Agents on the Quality of"Grey-matter"Hybrid Rice Seeds
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作者 Yuan YUAN Manzhuo TANG +7 位作者 Hanyu NING Lianghui JIANG Chunxia LU Yan WANG Chenzhong JIN Jie PENG Xinhua KUANG yihong hu 《Agricultural Biotechnology》 CAS 2023年第3期119-124,共6页
[Objectives]This study was conducted to select suitable mixed seed coating agents for improving the quality of"grey-matter"hybrid rice seeds.[Methods]Three different mixed seed coating agents(A,B,C)were used... [Objectives]This study was conducted to select suitable mixed seed coating agents for improving the quality of"grey-matter"hybrid rice seeds.[Methods]Three different mixed seed coating agents(A,B,C)were used to coat the seeds of two varieties(Taiyou 390 and Zhenliangyou 8612)of hybrid rice with different"grey-matter"content(5%,15%,25%),and the results were investigated and compared.[Results]The combinations of treatment B(seed coating agent A+Linong)and treatment C(Manshijin+seed coating agent A)could significantly improve indexes including seed germination potential,germination rate,seed vigor,seedling height,fibrous roots and fresh weight of the two varieties with a"grey-matter"content greater than 15%,but had no significant effects on main root length,dry weight,leaf number and tiller number,and the effects of treatment B was better than those of treatment C.That is to say,mixed seed coating agent B(seed coating agent A+Linong)was suitable for use as a seed coating agent to improve the quality of"grey-matter"seeds.[Conclusions]This study provides a reference plan for the safe use of mixed seed batches with"grey-matter"deterioration. 展开更多
关键词 Hybrid rice Grey-matter seed Seed coating agent Seed vigor Seedling quality
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Effects of Polygonatum odoratum Polysaccharide on Reproductive Hormones in Male Rats Fed a High-fat Diet
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作者 Yixin YAN Gang ZHANG +5 位作者 Lingli BAI Xin TANG Mengjun YUAN yihong hu Yan WANG Chenzhong JIN 《Agricultural Biotechnology》 CAS 2023年第2期54-58,共5页
[Objectives]This study was conducted to investigate the effects of Polygonatum odoratum polysaccharide(POP)on organ relative weights and reproductive hormone levels in male rats fed a high-fat diet.[Methods]Thirty hea... [Objectives]This study was conducted to investigate the effects of Polygonatum odoratum polysaccharide(POP)on organ relative weights and reproductive hormone levels in male rats fed a high-fat diet.[Methods]Thirty healthy male Sprague-Dawley(SD)rats were randomly divided into two groups according to their body weight:10 in normal control group(Group NC,n=10)and 20 in experimental group(n=20).The rats in experimental group were fed a high-fat diet for eight weeks before they were further randomly divided into two groups:high fat group(Group HF)and high fat+400 mg/(kg·d)POP group(Group HF+POP).In Group HF+POP,the rats were administered with POP for another six weeks,before their blood plasma was collected,and the relative weights of their testis and epididymis were calculated.The plasma levels of testosterone(T),estrogen(E2),follicle-stimulating hormone(FSH),cortisol(C)and luteinizing hormone(LH)were measured by radioimmunoassay,and the plasma levels of sex hormone-binding globulin(SHBG)and insulin-like growth factor-1(IGF-1)were determined by enzyme-linked immunosorbent assay.[Results]Compared with Group HF,POP could effectively inhibit rat obesity caused by high-fat diets,increase the relative weights of their testis and epididymis,plasma levels of LH,E2,FSH,T,SHBG and IGF-1,and reduce the plasma level of E2.[Conclusions]Polygonatum odoratum polysaccharide(POP)is able to effectively regulate the level of reproductive hormones in high-fat diet fed rats,and helps to protect their reproductive function. 展开更多
关键词 Polygonatum odoratum polysaccharide High-fat diet Reproductive hormones OBESITY SD rats
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Effects of Polygonatum sibiricum Polysaccharide (PSP) on Inflammatory Factors in Rats Fed on High-fat Diet
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作者 Mingzhe SU Mingjun LI +3 位作者 Lingli BAI Chenzhong JIN yihong hu Yan WANG 《Agricultural Biotechnology》 CAS 2023年第4期8-11,共4页
[Objectives]This study was conducted to explore the effects of Polygonatum sibiricum polysaccharide(PSP)on chronic intestinal inflammation caused by high-fat diet.[Methods]Thirty five male healthy SD rats were randoml... [Objectives]This study was conducted to explore the effects of Polygonatum sibiricum polysaccharide(PSP)on chronic intestinal inflammation caused by high-fat diet.[Methods]Thirty five male healthy SD rats were randomly divided into a normal control group(NC,normal diet,n=10)and a high-fat diet group(HF,high-fat diet,n=25).After 8 weeks,an obesity model was established.The HF group was randomly divided into an HF group and a PSP treatment group[PSP,300 mg/(kg·d)].After 6 weeks of intervention with PSP,rat serum was collected,and the spleen and thymus were stripped,and weighed.Serum IgG,IgM,LPS and IL-1βand IL-6 contents were detected by ELISA,and HE staining was adopted to observe the pathological changes of the colon tissue.[Results]PSP reduced the level of LPS caused by high-fat diet and the levels of inflammatory factors IL-6 and TNF-α,increased the indexes of the thymus and spleen serving as immune organs,increased IgG and IgM contents,and alleviated pathological damage to the colon tissue caused by high-fat diet.[Conclusions]This study provides a theoretical basis and experimental basis for the development of drugs for treating metabolic diseases such as obesity and inflammation. 展开更多
关键词 PSP High-fat diet OBESITY SD rats
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Effects of Polygonatum sibiricum Polysaccharide on Antioxidant Capacity of the Liver in High-fat Diet Rats
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作者 Heyi GONG Zhifeng LI +2 位作者 Chenzhong JIN yihong hu Yan WANG 《Agricultural Biotechnology》 CAS 2023年第4期12-15,24,共5页
[Objectives]This study was conducted to investigate the effects of Polygonatum sibiricum polysaccharide(PSP)on antioxidant function in high-fat diet obese rats.[Methods]Thirty five healthy male SD rats were selected t... [Objectives]This study was conducted to investigate the effects of Polygonatum sibiricum polysaccharide(PSP)on antioxidant function in high-fat diet obese rats.[Methods]Thirty five healthy male SD rats were selected to establish an obesity model after feeding a high-fat for 8 weeks.They were then randomly divided into a normal group(NC),a high-fat diet group(HF),and an HF+P.sibiricum polysaccharide group[HF+PSP,300 mg/(kg·d)].After 6 weeks of PSP intervention,the serum and liver of rats were collected,and the activity of aspartate transaminase(AST)and alanine aminotransferase(ALT)in serum,the enzyme activity of superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GSH-Px)and malondialdehyde(MDA)in liver tissue were measured.The pathological changes of liver tissue were observed by HE staining.[Results]Compared with the HF group,PSP could effectively inhibit obesity caused by high-fat diet.It reduced body weight and serum AST and ALT levels,increased the contents of T-SOD,CAT and GSH-Px in the liver,and inhibited the accumulation of MDA content,thereby reducing damage to liver cells caused by a high-fat diet.It indicated that PSP could effectively inhibit obesity in high-fat diet rats and enhance their antioxidant capacity.[Conclusions]This study provides a reference for the study of the antioxidant capacity of PSP. 展开更多
关键词 Polygonatum sibiricum polysaccharide High-fat diet OBESITY SD rat
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Effects of Polygonatum odoratum Polysaccharides on the Quality of White Chili Peppers in Jars
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作者 Xiuqiang WANG Li ZHOU +4 位作者 Chenzhong JIN yihong hu Jing QIU Yunyun ZHOU Yan WANG 《Agricultural Biotechnology》 CAS 2023年第4期37-40,共4页
[Objectives]This study was conducted to investigate the effects of Polygonatum odoratum polysaccharides on the quality of white chili peppers in jars.[Methods]White chili peppers were pickled by adding different conce... [Objectives]This study was conducted to investigate the effects of Polygonatum odoratum polysaccharides on the quality of white chili peppers in jars.[Methods]White chili peppers were pickled by adding different concentrations of P.odoratum polysaccharides with traditional pickling technology,and its nitrite content,pH and sensory quality were analyzed and detected.[Results]The results showed that,compared with the control group without adding P.odoratum polysaccharides,the nitrite content in white chili peppers in jars decreased after adding P.odoratum polysaccharides.When the addition amount of P.odoratum polysaccharides was 0.002%,the inhibition rate of nitrite reached the maximum.Under the same fermentation time,P.odoratum polysaccharides could significantly reduce the pH value of pickles.When the addition amount of P.odoratum polysaccharide was 0.002%and the pickling time was 3 months,the sensory quality of white chili peppers in jars was the best.At this point,white chili peppers in jars had rich aroma and tasted soft and sour,and the sour and salinity were suitable.As the fermentation time continued to increase,the chili aroma of white chili peppers in jars gradually decreased,and the sour taste became more pronounced.[Conclusions]This study can provide reference for the safety and quality control of white chili peppers in jars. 展开更多
关键词 Polygonatum odoratum polysaccharides White chili peppers in jars Pickling process QUALITY
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An Offloading Scheme Leveraging on Neighboring Node Resources for Edge Computing over Fiber-Wireless (FiWi) Access Networks 被引量:3
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作者 Wei Chang yihong hu +2 位作者 Guochu Shou Yaqiong Liu Zhigang Guo 《China Communications》 SCIE CSCD 2019年第11期107-119,共13页
The computation resources at a single node in Edge Computing(EC)are commonly limited,which cannot execute large scale computation tasks.To face the challenge,an Offloading scheme leveraging on NEighboring node Resourc... The computation resources at a single node in Edge Computing(EC)are commonly limited,which cannot execute large scale computation tasks.To face the challenge,an Offloading scheme leveraging on NEighboring node Resources(ONER)for EC over Fiber-Wireless(FiWi)access networks is proposed in this paper.In the ONER scheme,the FiWi network connects edge computing nodes with fiber and converges wireless and fiber connections seamlessly,so that it can support the offloading transmission with low delay and wide bandwidth.Based on the ONER scheme supported by FiWi networks,computation tasks can be offloaded to edge computing nodes in a wider range of area without increasing wireless hops(e.g.,just one wireless hop),which achieves low delay.Additionally,an efficient Computation Resource Scheduling(CRS)algorithm based on the ONER scheme is also proposed to make offloading decision.The results show that more offloading requests can be satisfied and the average completion time of computation tasks decreases significantly with the ONER scheme and the CRS algorithm.Therefore,the ONER scheme and the CRS algorithm can schedule computation resources at neighboring edge computing nodes for offloading to meet the challenge of large scale computation tasks. 展开更多
关键词 EDGE COMPUTING OFFLOADING Fiber-wireless access networks delay
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Effects of Different Proportions of Spent Pleurotus ostreatus Substrate on Seed Germination and Seedling Growth of Mung Beans 被引量:1
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作者 Ling WANG Shuang YANG +2 位作者 yihong hu Chenzhong JIN Zhi ZENG 《Agricultural Biotechnology》 CAS 2019年第2期81-85,共5页
[Objectives] This study was conducted to investigate the effects of different proportions of spent Pleurotus ostreatus substrate on the germination and seedling growth of mung beans. [Methods] The cellulose-degrading ... [Objectives] This study was conducted to investigate the effects of different proportions of spent Pleurotus ostreatus substrate on the germination and seedling growth of mung beans. [Methods] The cellulose-degrading bacteria HB8 and HF1 were mixed with a commercially available microbial composting agent, respectively, for the composting of spent P. ostreatus substrate. Mung beans were cultivated with different proportions of spent mushroom substrate compost and soil. The seed germination rate, plant height, fresh weight and chlorophyll content of mung bean were used as indicators to investigate the effects of the organic fertilizer from spent P. ostreatus substrate on the growth of mung bean seedlings. [Results] The addition of cellulose-degrading bacteria can significantly improve the composting effect of the spent mushroom substrate. After 8 d of cultivation of mung beans with different ratios of the mushroom substrate organic fertilizer, 50% of the organic fertilizer can make the plant height, fresh weight and leaf chlorophyll content of mung bean seedlings reach the highest value and was suitable for mung bean breeding and cultivation. [Conclusions] This study provides scientific basis and technical indicators for the rapid and harmless treatment of spent mushroom substrate and its application in crop cultivation and nursery. 展开更多
关键词 Cellulose-degrading bacteria Organic fertilizer from SPENT PLEUROTUS ostreatus SUBSTRATE MUNG bean Seed germination rate
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Synthetic protease inhibitor-induced inclusions in PC12 cells Potential proteomic characterization of six subunits in the 26S proteasome
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作者 Mingxiu Tian Xing'an Li +6 位作者 Yingjiu Zhang yihong hu Ming Chang Tao Liu Danping Wang Yu Zhang Linsen hu 《Neural Regeneration Research》 SCIE CAS CSCD 2010年第22期1685-1693,共9页
Proteasome dysfunction during dopaminergic degeneration induces proteolytic stress, and is a contributing factor for the onset and formation of Lewy bodies. Results from our previous studies showed that synthetic prot... Proteasome dysfunction during dopaminergic degeneration induces proteolytic stress, and is a contributing factor for the onset and formation of Lewy bodies. Results from our previous studies showed that synthetic proteasome inhibitor-induced inclusions in PC12 cells contained six subunits in the 26S proteasome. In the present study, mass spectrometry analysis of single protein spots resolved by two-dimensional gel electrophoresis and identified by bioinformatic analysis of peptide mass fingerprint (PMF) data were performed to comprehensively characterize the proteomic profile of the proteasome subunits. Results showed that six subunits in the 26S proteasome were characterized through accurate assignment by PMF data-specific protein identification in protein databases. Additionally, identification of one of the proteasome subunits was further confirmed using a subunit-specific antibody against non-adenosine triphosphatase subunit 11 of the 19S regulatory particle. Results suggest that the potential proteomic profile of six subunits in the 26S proteasome could be established from proteasome inhibitor-induced inclusions in PC12 cells. 展开更多
关键词 PC12 cells proteasome inhibitor-induced inclusions proteasome subunit two-dimensional gel peptide mass fingerprints PROTEOMIC
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Optimization of Extraction of Saponins from Tribulus terrestris L.
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作者 Quan YU Zhisheng LI +3 位作者 Yuan ZENG Chenzhong JIN Yan WANG yihong hu 《Agricultural Biotechnology》 CAS 2019年第3期162-165,168,共5页
To promote the recovery of saponins from Tribulus terrestris L.,the water dissolution and ethanol precipitation method was adopted to extract saponins from the powder prepared from T.terrestris fruit.The optimal proce... To promote the recovery of saponins from Tribulus terrestris L.,the water dissolution and ethanol precipitation method was adopted to extract saponins from the powder prepared from T.terrestris fruit.The optimal process was to extract with 9 fold of the extracting water at the ethanol concentration of 85 for 3 times,each time 3.5 h.The effects of the factors ranked as the extracting water quantity > treatment time > treatment times > ethanol concentration.Under these optimal conditions,the recovery of saponins was 2.81%.After these crude saponins were purified by D101 macroporous adsorptive resin,the purity was found to be promoted through the UV scanning method,and the recovery of the refined saponins was up to 1.53%.Both of the recovery values were higher than that of the existing process. 展开更多
关键词 Tribulus terrestris L. SAPONINS ORTHOGONAL experiment RECOVERY MACROPOROUS ADSORPTIVE RESIN
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Optimization of Enzymatic Extraction of Sodium Chondroitin Sulfate From Bovine Nasal Bone
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作者 Jinmao TAN Shunli XIAO +7 位作者 An TAN Gaoyuan ZOU Shuanghui WANG yihong hu Yizhi Zhu Chenzhong JIN Na WU Yunyun ZHOU 《Agricultural Biotechnology》 CAS 2019年第4期186-189,共4页
Taking bovine nasal bone as a raw material,the sodium chondroitin sulfate was extracted by enzymolysis-ion adsorption-alcohol precipitation method,and the enzymolysis process was optimized.After single factor experime... Taking bovine nasal bone as a raw material,the sodium chondroitin sulfate was extracted by enzymolysis-ion adsorption-alcohol precipitation method,and the enzymolysis process was optimized.After single factor experiments,the response surface analysis was performed by software Design-Expert 8.0,and the optimal enzymolysis conditions were as follows:the trypsin concentration of 0.8%,the pH value of 9.0,the enzymolysis temperature at 52℃,and the enzymolysis time of 2.0 h.With this combination of conditions,the concentration of sodium chondroitin sulfate extract reached 11.3 g/L,the bovine nasal bone yield was 35%,and the finished product content was 92.5%,which provided guidance for industrial production. 展开更多
关键词 SODIUM CHONDROITIN sulfate TRYPSIN PHOTOMETRIC TITRATION Response surface methodology
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Study on the Toxicity of Acetochlor to Loaches
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作者 Yan WANG Peng DONG +7 位作者 Pei XU Yuxiang LIN Zexin CHEN yihong hu Chenzhong JIN Yiding WANG Kaifa GUO Qianxia Zhu 《Agricultural Biotechnology》 CAS 2020年第3期49-52,66,共5页
[Objectives] This study was conducted to investigate the toxic effect of acetochlor in loaches. [Methods]Loaches were put into the acetochlor solution with concentrations of 0,0. 062 5,0. 125,0. 25,0. 5 and 1 μl/L,re... [Objectives] This study was conducted to investigate the toxic effect of acetochlor in loaches. [Methods]Loaches were put into the acetochlor solution with concentrations of 0,0. 062 5,0. 125,0. 25,0. 5 and 1 μl/L,respectively. The effects on dismutase( SOD),peroxidase( POD),catalase( CAT) and glutathione( GSH) activity and malondialdehyde( MDA) content in the liver of loaches were analyzed after 12,24,48 and 72 h of exposure. [Results] The activity of SOD,POD,CAT and GSH increased with the stress time and concentration,reached their maximums at 48 h after acetochlor treatment,and then decreased.However,MDA content increased with the stress time and concentration. [Conclusions] Acetochlor had obvious oxidative stress on loaches,and the activity of SOD,POD,CAT and GSH showed an obvious induction-inhibition effect with the increase of acetochlor concentration and the extension of stress time. 展开更多
关键词 ACETOCHLOR LOACH Antioxidative enzyme system MALONDIALDEHYDE
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Research Progress on Biological Characteristics and Integrated Management of Digitaria sanguinalis
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作者 Wenxin JIN yihong hu +5 位作者 Chenzhong JIN Yunyun ZHOU Xuejiao ZHANG Yan WANG Shuanghui WANG Xiu LIU 《Agricultural Biotechnology》 CAS 2018年第6期85-88,共4页
Digitaria sanguinalis is an annual worst weed in the grass family that occurs in all parts of China.It mainly does harm to dryland crops and direct-sowing rice fields.In agricultural production,chemical herbicides are... Digitaria sanguinalis is an annual worst weed in the grass family that occurs in all parts of China.It mainly does harm to dryland crops and direct-sowing rice fields.In agricultural production,chemical herbicides are mainly used to control them,but the problems of environmental pollution and drug resistance caused by chemical herbicides have become more and more severe,which is in urgent need of integrated management.In this paper,the biological characteristics,chemical control,microbial control and allelopathy of D.sanguinalis were reviewed. 展开更多
关键词 Digitaria sanguinalis CHEMICAL HERBICIDE MICROBIAL CONTROL Allelopathic EFFECT
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Optimization of Ultrasonic-assisted Extraction of Polysaccharide from Hibiscus syriacus L.
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作者 Haibo DENG Bei NING +3 位作者 Shunfan Zhu Chenzhong JIN yihong hu Xuejiao ZHANG 《Agricultural Biotechnology》 CAS 2018年第3期161-164,共4页
The ultrasonic-assisted extraction of polysaccharides from Hibiscus syriacus L. at 180 W low power was optimized by the single factor tests and orthogonal test. Extraction temperature,ultrasonic treatment time,extract... The ultrasonic-assisted extraction of polysaccharides from Hibiscus syriacus L. at 180 W low power was optimized by the single factor tests and orthogonal test. Extraction temperature,ultrasonic treatment time,extraction times,and material-to-liquid ratio were considered as independent variables. The results suggested that the best extraction condition were determined as the extraction temperature at 80 ℃,the ultrasonic treatment time of 40 min,the material-to-liquid ratio at1∶ 20( g/ml),and the extraction times of 3. Under the optimal conditions,the extraction rate of polysaccharides from Hibiscus syriacus was 3. 114%. 展开更多
关键词 Hibiscus syriacus L. POLYSACCHARIDE Ultrasound-assisted extraction
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National Epidemiology and Evolutionary History of Four Hand, Foot and Mouth Disease-Related Enteroviruses in China from 2008 to 2016 被引量:40
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作者 Xuemin Fu Zhenzhou Wan +3 位作者 Yanpeng Li yihong hu Xia Jin Chiyu Zhang 《Virologica Sinica》 SCIE CAS CSCD 2020年第1期21-33,共13页
Hand, foot and mouth disease(HFMD) is a major public health concern in China. The most predominant enteroviruses that cause HFMD have traditionally been attributed to enterovirus A71(EVA71) and coxsackievirus A16(CVA1... Hand, foot and mouth disease(HFMD) is a major public health concern in China. The most predominant enteroviruses that cause HFMD have traditionally been attributed to enterovirus A71(EVA71) and coxsackievirus A16(CVA16). Since its first large outbreak in 2008, the dominant HFMD pathogens are constantly changing. In 2013 and 2015, CVA6 exceeded both EVA71 and CVA16 to become the leading cause of HFMD in some provinces. However, there still lacks a comprehensive overview on the molecular epidemiology and evolution of HFMD-related enteroviruses at the national level. In this study, we performed systematic epidemiological analyses of HFMD-related enteroviruses using the data of 64 published papers that met the inclusion criteria, and conducted phylogenetic analyses based on 12,080 partial VP1 sequences identified in China before 31 st June 2018. We found that EVA71 prevalence has decreased sharply but other enteroviruses have increased rapidly from 2008 to 2016 and that one subtype of each enterovirus is represented during the epidemic. In addition, four genotypes EVA71_C4, CVA16_B1, CVA6_D and CVA10_C are the most predominant enterovirus strains and collectively they cause over 90% of all HFMD cases in China according to the phylogenetic trees using representative partial VP1 sequences. These four major enterovirus genotypes have different geographical distributions, and they may cocirculate with other genotypes and serotypes. These results suggest that more molecular epidemiological studies should be performed on several enteroviruses simultaneously, and such information should have implications for virological surveillance, disease management, vaccine development and policy-making on the prevention and control of HFMD. 展开更多
关键词 ENTEROVIRUS Hand foot and MOUTH disease(HFMD) Molecular EPIDEMIOLOGY Evolution Genotype
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THE BOUND OF PRICE OF ANARCHY FOR MULTI-CLASS AND MULTI-CRITERIA TRAFFIC EQUILIBRIUM PROBLEM
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作者 Kedong CHEN Daoli Zhu +1 位作者 yihong hu Jianlin LIU 《Journal of Systems Science and Systems Engineering》 SCIE EI CSCD 2012年第1期77-93,共17页
In this paper, we use the variational method to study the efficiency loss of user equilibrium for the multi-class, multi-criterion traffic equilibrium with general tolls and a discrete set of value of. time. By introd... In this paper, we use the variational method to study the efficiency loss of user equilibrium for the multi-class, multi-criterion traffic equilibrium with general tolls and a discrete set of value of. time. By introducing three important parameters k1, k2, k3, we derive several bounds of price of anarchy for this problem when tolls are considered and not considered as part of the system cost, with the cost-based criterion. 展开更多
关键词 Value of time variational method price of anarchy multi-class multi-criterion traffic equilibrium
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