Under the guidance of strain tolerance, a new combination method for crude oil-degrading bacterial consortium was studied. Firstly, more than 50 efficient crude oil-degrading and biosurfactant producing bacteria were ...Under the guidance of strain tolerance, a new combination method for crude oil-degrading bacterial consortium was studied. Firstly, more than 50 efficient crude oil-degrading and biosurfactant producing bacteria were isolated from petroleum-contaminated soil and water in Tianjin Binhai New Area Oil field, China. Twenty-four of them were selected for further study. These strains were identified as belonging Pseudornonas aeruginosa, Bacillus subtilis, Brevibacillus brevis, Achrornobacter sp., Acinetobacter venetianus, Lysinibacillus rnacroides, Klebsiella oxytoca, Stenotrophornonas rhizophila, Rhodococcus sp. and Bacillus thuringiensis. A shake-flask degradation test revealed that 12 of these strains could degrade over 50% of 1% crude oil concentration in 7 d. Of these, 8 strains were able to produce biosurfactants. Furthermore, environmental tolerance experiments indicated that the majority of the strains had the ability to adapt to extreme environments including high temperatures, alkaline en- vironments and high salinity environments. A mixed bacterial agent comprising the strains WB2, W2, W3 and HA was developed based on the environmental tolerance tests and subjected to the pilot-scale degradation test indicating that this bacterial agent could degrade 85.2% of 0.8% crude oil concentration in 60 d. Our results suggest that the application of this mixed agent could remediate crude oil polluted soils in saline and alkaline environments.展开更多
The methane bubble plume attracts interest because it offers direct evidence of seafloor gas leakage and plays an indirect role in the exploration and identification of natural gas hydrate.In this study,based on estab...The methane bubble plume attracts interest because it offers direct evidence of seafloor gas leakage and plays an indirect role in the exploration and identification of natural gas hydrate.In this study,based on established plume models and their migration sections,three amplitude-class attributes were extracted from three formations for the migration sections of five plumes,and the correlation between the gas content and seismic attribute was obtained.As the gas content increases,the amplitude attribute correspondingly increases,and the linear correlation is relatively good.Moreover,correlation coefficients between gas content and amplitude attributes are close to 1.0.By using linear fitting,the relation model between the gas content of the plume and the seismic attribute was obtained.The relation model was subsequently used to invert the gas content from a real databearing plume.Comparison of the gas content section of the plume with the attribute section and real seismic section reveals common distribution characteristics,namely,the color of the section in the lower right corner is dark.If the amplitude value is large in the seismic section of the real plume,the amplitude attribute value is also large in the corresponding attribute section,and the inverted value of the gas content is also large(because gas content and amplitude are linearly correlated),which indicates that the plume bubbles of the section in the lower right corner is intensively distributed.Finally,the obtained gas content section of the plume can reflect the distribution of the plume bubble content more simply and intuitively,from which the distribution law of seafloor bubbles can be deduced,and this lays a foundation for the further estimation of the gas content of the plume and hydrate reserves.展开更多
Seed weight is a component of seed yield in rapeseed(Brassica napus L.).Although quantitative trait loci(QTL)for seed weight have been reported in rapeseed,only a few causal quantitative trait genes(QTGs)have been ide...Seed weight is a component of seed yield in rapeseed(Brassica napus L.).Although quantitative trait loci(QTL)for seed weight have been reported in rapeseed,only a few causal quantitative trait genes(QTGs)have been identified,resulting in a limitation in understanding of seed weight regulation.We constructed a gene coexpression network at the early seed developmental stage using transcripts of 20,408 genes in QTL intervals and 1017 rapeseed homologs of known genes from other species.Among the 10 modules in this gene coexpression network,modules 1 and 2 were core modules and contained genes involved in source–flow–sink processes such as synthesis and transportation of fatty acid and protein,and photosynthesis.A hub gene SERINE CARBOXYPEPTIDASE-LIKE 19(SCPL19)was identified by candidate gene association analysis in rapeseed and functionally investigated using Arabidopsis T-DNA mutant and overexpression lines.Our study demonstrates the power of gene coexpression analysis to prioritize candidate genes from large candidate QTG sets and enhances the understanding of molecular mechanism for seed weight at the early developmental stage in rapeseed.展开更多
Seed number per silique(SNPS)is one of seed yield components in rapeseed,but its genetic mechanism remains elusive.Here a double haploid(DH)population derived from a hybrid between female 6Q006with 35–40 SNPS and mal...Seed number per silique(SNPS)is one of seed yield components in rapeseed,but its genetic mechanism remains elusive.Here a double haploid(DH)population derived from a hybrid between female 6Q006with 35–40 SNPS and male 6W26 with 10–15 SNPS was investigated for SNPS in the year 2017,2018,2019 and 2021,and genotyped with Brassica 60K Illumina Infinium SNP array.An overlapping major QTL(qSNPS.C09)explaining 51.50%of phenotypic variance on average was narrowed to a 0.90 Mb region from 44.87 Mb to 45.77 Mb on chromosome C09 by BSA-seq.Subsequently,two DEGs in this interval were detected between extreme individuals in DH and F_2populations by transcriptome sequencing at7 and 14 days after pollination siliques.Of which,BnaC09g45400D encoded an adenine phosphoribosyltransferase 5(APT5)has a 48-bp InDel variation in the promoter of two parents.Candidate gene association analysis showed that this InDel variation was associated with SNPS in a nature population of rapeseed,where 54 accessions carrying the same haplotype as parent 6Q006 had higher SNPS than103 accessions carrying the same haplotype as parent 6W26.Collectively,the findings are helpful for rapeseed molecular breeding of SNPS,and provide new insight into the genetic and molecular mechanism of SNPS in rapeseed.展开更多
Over the past decades,sea ice in the polar regions has been significantly affecting local and even hemispheric climate through a positive ice albedo feedback mechanism.The role of fast ice,as opposed to drift ice,has ...Over the past decades,sea ice in the polar regions has been significantly affecting local and even hemispheric climate through a positive ice albedo feedback mechanism.The role of fast ice,as opposed to drift ice,has not been well-studied due to its relatively small coverage over the earth.In this paper,the optical properties and surface energy balance of land fast ice in spring are studied using in situ observations in Barrow,Alaska.The results show that the albedo of the fast ice varied between 0.57 and 0.85 while the transmittance increased from 1.3×10-3 to 4.1×10-3 during the observation period.Snowfall and air temperature affected the albedo and absorbance of sea ice,but the transmittance had no obvious relationship with precipitation or snow cover.Net solar shortwave radiation contributes to the surface energy balance with a positive 99.2%of the incident flux,with sensible heat flux for the remaining 0.8%.Meanwhile,the ice surface loses energy through the net longwave radiation by 18.7%of the total emission,while the latent heat flux accounts for only 0.1%.Heat conduction is also an important factor in the overall energy budget of sea ice,contributing 81.2%of the energy loss.Results of the radiative transfer model reveal that the spectral transmittance of the fast ice is determined by the thickness of snow and sea ice as well as the amount of inclusions.As major inclusions,the ice biota and particulates have a significant influence on the magnitude and distribution of the spectral transmittance.Based on the radiative transfer model,concentrations of chlorophyll and particulate in the fast ice are estimated at 5.51 mg/m^(2)and 95.79 g/m^(2),which are typical values in the spring in Barrow.展开更多
Graphene’s optical absorption coefficient increases linearly with the number of layers making it more effective in the construction of optical tuning graphene-based devices. Refractive index(RI) is one of the importa...Graphene’s optical absorption coefficient increases linearly with the number of layers making it more effective in the construction of optical tuning graphene-based devices. Refractive index(RI) is one of the important optical parameters of the graphene for accurately describing its optical characteristics and further applications. In view of the RI research of the multilayer graphene is lacking and existing measurement methods are complicated. Optical power tuning RI of multilayer graphene is investigated using a simple measurement and no temperature cross sensitivity all optical fiber sensing structure.Optical power tuning RI characteristics of multilayer graphene are studied by tuning the introducing broad band light power from 0.57 mW to 22.7 m W. Different thickness graphene coating shows different tuning efficiency. At 4.86-μm thickness,a 3.433-nm Bragg wavelength shift is obtained with 156.2-pm/mW wavelength versus optical power tuning sensitivity corresponding to 3.25×10~3 RI change and 0.154 URI/W(URI, unit of RI) RI optical power tuning efficiency.展开更多
Oceanic vertical mixing of the lower halocline water(LHW)in the Chukchi Borderland and Mendeleyev Ridge was studied based on in situ hydrographic and turbulent observations.The depth-averaged turbulent dissipation rat...Oceanic vertical mixing of the lower halocline water(LHW)in the Chukchi Borderland and Mendeleyev Ridge was studied based on in situ hydrographic and turbulent observations.The depth-averaged turbulent dissipation rate of LHW demonstrates a clear topographic dependence,with a mean value of 1.2×10^(-9) W/kg in the southwest of Canada Basin,1.5×10^(-9) W/kg in the Mendeleyev Abyssal Plain,2.4×10^(-9) W/kg on the Mendeleyev Ridge,and2.7×10^(-9) W/kg on the Chukchi Cap.Correspondingly,the mean depth-averaged vertical heat flux of the LHW is0.21 W/m^(2) in the southwest Canada Basin,0.30 W/m^(2) in the Mendeleyev Abyssal Plain,0.39 W/m^(2) on the Mendeleyev Ridge,and 0.46 W/m^(2) on the Chukchi Cap.However,in the presence of Pacific Winter Water,the upward heat released from Atlantic Water through the lower halocline can hardly contribute to the surface ocean.Further,the underlying mechanisms of diapycnal mixing in LHW—double diffusion and shear instability—was investigated.The mixing in LHW where double diffusion were observed is always relatively weaker,with corresponding dissipation rate ranging from 1.01×10^(-9) W/kg to 1.57×10^(-9) W/kg.The results also show a strong correlation between the depth-average dissipation rate and strain variance in the LHW,which indicates a close physical linkage between the turbulent mixing and internal wave activities.In addition,both surface wind forcing and semidiurnal tides significantly contribute to the turbulent mixing in the LHW.展开更多
As a part of certain region,agricultural development has inevitable connection with regional urbanization,and they are highly heterogeneous and complementary in space. Their internal relationship is manifested as agri...As a part of certain region,agricultural development has inevitable connection with regional urbanization,and they are highly heterogeneous and complementary in space. Their internal relationship is manifested as agricultural structure suits demand of urbanization development through adjustment in the process of urbanization,while urbanization is based on development of regional agriculture. From history of world agriculture and urbanization development,agriculture firstly provides primitive accumulation for urbanization development,and drives regional urbanization through promoting agricultural development. When urbanization develops to certain stage,urbanization accumulation increases and reaches the goal of " boosting urbanization through agriculture". If urbanization develops slowly,agricultural adjustment will lack motive force. On the contrary,weak agricultural development will lead to stagnation of urbanization. Therefore,adjustment of agricultural structure and urbanization development are interconnected,mutually promoted and restricted,and interdependent.展开更多
Background In recent decades,unmanned aerial vehicles(UAVs)have developed rapidly and been widely applied in many domains,including photography,reconstruction,monitoring,and search and rescue.In such applications,one ...Background In recent decades,unmanned aerial vehicles(UAVs)have developed rapidly and been widely applied in many domains,including photography,reconstruction,monitoring,and search and rescue.In such applications,one key issue is path and view planning,which tells UAVs exactly where to fly and how to search.Methods With specific consideration for three popular UAV applications(scene reconstruction,environment exploration,and aerial cinematography),we present a survey that should assist researchers in positioning and evaluating their works in the context of existing solutions.Results/Conclusions It should also help newcomers and practitioners in related fields quickly gain an overview of the vast literature.In addition to the current research status,we analyze and elaborate on advantages,disadvantages,and potential explorative trends for each application domain.展开更多
This work describes intermolecular acylfluorination of gem-difluoroenynes using acyl fluorides as both acyl source and fluorine source.Trifluoromethyl-substituted allenones or furans could be selectively achieved via ...This work describes intermolecular acylfluorination of gem-difluoroenynes using acyl fluorides as both acyl source and fluorine source.Trifluoromethyl-substituted allenones or furans could be selectively achieved via phosphine and silver catalysis.These approaches exhibit high regioselectivity,atom econ-omy and broad functionality tolerance.展开更多
Surface acoustic wave(SAW)resonators based on lithium tantalate(LT,LiTaO_(3))wafers are crucial elements of mobile communication filters.The use of intrinsic LT wafers typically brings about low fabrication accuracy o...Surface acoustic wave(SAW)resonators based on lithium tantalate(LT,LiTaO_(3))wafers are crucial elements of mobile communication filters.The use of intrinsic LT wafers typically brings about low fabrication accuracy of SAW resonators due to strong UV reflection in the lithography process.This hinders their resonance frequency control seriously in industrial manufacture.LT doping and chemical reduction could be applied to decrease the UV reflection of LT wafers for high lithographic precision.However,conventional methods fail to provide a fast and nondestructive approach to identify the UV performance of standard single-side polished LT wafers for highprecision frequency control.Here,we propose a convenient on-line sensing scheme based on the colorimetry of reduced Fe-doped LT wafers and build up an automatic testing system for industrial applications.The levels of Fe doping and chemical reduction are evaluated by the lightness and color difference of LT-based wafers.The correlation between the wafer visible colorimetry and UV reflection is established to refine the lithography process and specifically manipulate the frequency performance of SAW resonators.Our study provides a powerful tool for the fabrication control of SAW resonators and will inspire more applications on sophisticated devices of mobile communication.展开更多
The authors introduce the intactness-aware Mosaic data augmentation strategy,designed to tackle challenges such as low accuracy in detecting defects in insulation pull rods,limited timeliness in intelligent analysis,a...The authors introduce the intactness-aware Mosaic data augmentation strategy,designed to tackle challenges such as low accuracy in detecting defects in insulation pull rods,limited timeliness in intelligent analysis,and the absence of a comprehensive database for information on insulation pull rod defects.The proposed strategy incorporates the YOLOv5s algorithm for detecting defects in insulation pull rods.Initially,the YOLOv5s network was constructed,and a dataset containing photos of insulation pull rods with white spots,fractures,impurities,and bubble flaws was compiled to capture images of defects.The research presented a data enhancement approach to improve the images and establish a dataset for insulation pull rod defects.The YOLOv5s algorithm was applied for both training and testing purposes.A comparative analysis was conducted to assess the detection performance of YOLOv5s against a conventional target detector for identifying defects in insulation pull rods.Furthermore,the utility of Mosaic's data augmentation technique,which incorporates intactness awareness,was evaluated to enhance the accuracy of identifying insulation pull rod defects.The research findings indicate that the YOLOv5s algorithm is employed for intelligent detection and precise localisation of flaws.The intactnessaware Mosaic data augmentation strategy significantly improves the accuracy of detecting faults in insulation pull rods.The YOLOv5s model used achieves a performance index mAP@0.5:0.95 of 0.563 on the test set,distinct from the training set data.With a threshold of 0.5,the mAP@0.5 score is 0.904,indicating a substantial improvement in both detection efficiency and accuracy compared to conventional target detection methods.Innovative approaches for identifying defects in insulation pull rods are introduced.展开更多
Benzotriazole(BTA), an anticorrosion agent of slurry, is the main organic pollutant remaining after CMP of multilayer copper wiring, and also the main removal object of post CMP cleaning. The adsorption of BTA onto th...Benzotriazole(BTA), an anticorrosion agent of slurry, is the main organic pollutant remaining after CMP of multilayer copper wiring, and also the main removal object of post CMP cleaning. The adsorption of BTA onto the copper could form a dense Cu-BTA film, which makes the copper surface strongly passivated. According to this characteristic, quantitative analysis of BTA residue after cleaning is carried out by contact angle measurement and electrochemical measurement in this paper. A scanning electron microscope(SEM) with EDX was used to observe and analyze the BTA shape and elements. The efficiencies of organic alkali and inorganic alkali on the removal of BTA were studied. The corresponding reaction mechanism was also analyzed. The results show that the adsorption structure of Cu(I)-BTA cannot be destroyed in an alkaline environment with a pH less than 10; the effect of BTA removal by inorganic alkali is worse than that of the organic amine alkali with the coordination structure under the same pH environment; the FA/O Ⅱ chelating agent with the fraction of 200 ppm can effectively remove BTA residue on the surface of copper wafer.展开更多
Detection of human immunodeficiency virus(HIV)p24 protein at a single pg/ml concentration in point-of-care(POC)settings is important because it can facilitate acute HIV infection diagnosis with a detection sensitivity...Detection of human immunodeficiency virus(HIV)p24 protein at a single pg/ml concentration in point-of-care(POC)settings is important because it can facilitate acute HIV infection diagnosis with a detection sensitivity approaching that of laboratory-based assays.However,the limit of detection(LOD)of lateral flow immunoassays(LFAs),the most prominent POC diagnostic platform,falls short of that of laboratory protein detection methods such as enzyme-linked immunosorbent assay(ELISA).Here,we report the development and optimization of a thermal contrast amplification(TCA)LFA that will allow ultrasensitive detection of 8 pg/ml p24 protein spiked into human serum at POC,approaching the LOD of a laboratory test.To achieve this aim,we pursued several innovations as follows:(a)defining a new quantitative figure of merit for LFA design based on the specific to nonspecific binding ratio(BR);(b)using different sizes and shapes of gold nanoparticles(GNPs)in the systematic optimization of TCA LFA designs;and(c)exploring new laser wavelengths and power regimes for TCA LFA designs.First,we optimized the blocking buffer for the membrane and running buffer by quantitatively measuring the BR using a TCA reader.The TCA reader interprets the thermal signal(i.e.,temperature)of GNPs within the membrane when irradiated by a laser at the plasmon resonance wavelength of the particle.This process results in higher detection and quantitation of GNPs than in traditional visual detection(i.e.,color intensity).Further,we investigated the effect of laser power(30,100,200 mW),GNP size and shape(30 and 100 nm gold spheres,150 nm gold-silica shells),and laser wavelength(532,800 nm).Applying these innovations to a new TCA LFA design,we demonstrated that 100nm spheres with a 100 mW 532 nm laser provided the best performance(i.e.,LOD=8pg/ml).This LOD is significantly better than that of the current colorimetric LFA and is in the range of the laboratory-based p24 ELISA.In summary,this TCA LFA for p24 protein shows promise for detecting acute HIV infection in POC settings.展开更多
Optical microcavities play a significant role in the study of classical and quantum chaos.To date,most experimental explorations of their internal wave dynamics have focused on the properties of their inputs and outpu...Optical microcavities play a significant role in the study of classical and quantum chaos.To date,most experimental explorations of their internal wave dynamics have focused on the properties of their inputs and outputs,without directly interrogating the dynamics and the associated mode patterns inside.As a result,this key information is rarely retrieved with certainty,which significantly restricts the verification and understanding of the actual chaotic motion.Here we demonstrate a simple and robust approach to directly and rapidly map the internal mode patterns in chaotic microcavities.By introducing a local index perturbation through a pump laser,we report a spectral response of optical microcavities that is proportional to the internal field distribution.With this technique,chaotic modes with staggered mode spacings can be distinguished.Consequently,a complete chaos assisted tunneling(CAT)and its time-reversed process are experimentally verified in the optical domain with unprecedented certainty.展开更多
基金Supported by the National Basic Research Program of China(“973”Program:2014CB745100)the National Natural Science Foundation of China(21576197)+2 种基金Tianjin Research Program of Application Foundation and Advanced Technology(14JCQNJC06700)the Major Research Plan of Tianjin(16YFXTSF00460)Tianjin Penglai 19-3 Oil Spill Accident Compensation Project(19-3BC2014-03)
文摘Under the guidance of strain tolerance, a new combination method for crude oil-degrading bacterial consortium was studied. Firstly, more than 50 efficient crude oil-degrading and biosurfactant producing bacteria were isolated from petroleum-contaminated soil and water in Tianjin Binhai New Area Oil field, China. Twenty-four of them were selected for further study. These strains were identified as belonging Pseudornonas aeruginosa, Bacillus subtilis, Brevibacillus brevis, Achrornobacter sp., Acinetobacter venetianus, Lysinibacillus rnacroides, Klebsiella oxytoca, Stenotrophornonas rhizophila, Rhodococcus sp. and Bacillus thuringiensis. A shake-flask degradation test revealed that 12 of these strains could degrade over 50% of 1% crude oil concentration in 7 d. Of these, 8 strains were able to produce biosurfactants. Furthermore, environmental tolerance experiments indicated that the majority of the strains had the ability to adapt to extreme environments including high temperatures, alkaline en- vironments and high salinity environments. A mixed bacterial agent comprising the strains WB2, W2, W3 and HA was developed based on the environmental tolerance tests and subjected to the pilot-scale degradation test indicating that this bacterial agent could degrade 85.2% of 0.8% crude oil concentration in 60 d. Our results suggest that the application of this mixed agent could remediate crude oil polluted soils in saline and alkaline environments.
基金The Innovation and Enhancing School Project of Guangdong Ocean University under contract No.230419096the Joint Research on Exploration and Development Technology of Natural Gas Hydrate under contract No.2018YFE0208200+2 种基金the Teaching Team Project of Guangdong Ocean University under contract No.570220033the National Natural Science Fundation of China under contract Nos 42004103 and 41306050the Fund of Southern Marine Science and Engineering Guangdong Laboratory(Zhanjiang)under contract No.ZJW-2019-08。
文摘The methane bubble plume attracts interest because it offers direct evidence of seafloor gas leakage and plays an indirect role in the exploration and identification of natural gas hydrate.In this study,based on established plume models and their migration sections,three amplitude-class attributes were extracted from three formations for the migration sections of five plumes,and the correlation between the gas content and seismic attribute was obtained.As the gas content increases,the amplitude attribute correspondingly increases,and the linear correlation is relatively good.Moreover,correlation coefficients between gas content and amplitude attributes are close to 1.0.By using linear fitting,the relation model between the gas content of the plume and the seismic attribute was obtained.The relation model was subsequently used to invert the gas content from a real databearing plume.Comparison of the gas content section of the plume with the attribute section and real seismic section reveals common distribution characteristics,namely,the color of the section in the lower right corner is dark.If the amplitude value is large in the seismic section of the real plume,the amplitude attribute value is also large in the corresponding attribute section,and the inverted value of the gas content is also large(because gas content and amplitude are linearly correlated),which indicates that the plume bubbles of the section in the lower right corner is intensively distributed.Finally,the obtained gas content section of the plume can reflect the distribution of the plume bubble content more simply and intuitively,from which the distribution law of seafloor bubbles can be deduced,and this lays a foundation for the further estimation of the gas content of the plume and hydrate reserves.
基金provided by the National Natural Science Foundation of China(32201776)the Natural Science Foundation of Chongqing(cstc2019jcyj-bsh X0055,cstc2019jcyj-zdxm X0012)。
文摘Seed weight is a component of seed yield in rapeseed(Brassica napus L.).Although quantitative trait loci(QTL)for seed weight have been reported in rapeseed,only a few causal quantitative trait genes(QTGs)have been identified,resulting in a limitation in understanding of seed weight regulation.We constructed a gene coexpression network at the early seed developmental stage using transcripts of 20,408 genes in QTL intervals and 1017 rapeseed homologs of known genes from other species.Among the 10 modules in this gene coexpression network,modules 1 and 2 were core modules and contained genes involved in source–flow–sink processes such as synthesis and transportation of fatty acid and protein,and photosynthesis.A hub gene SERINE CARBOXYPEPTIDASE-LIKE 19(SCPL19)was identified by candidate gene association analysis in rapeseed and functionally investigated using Arabidopsis T-DNA mutant and overexpression lines.Our study demonstrates the power of gene coexpression analysis to prioritize candidate genes from large candidate QTG sets and enhances the understanding of molecular mechanism for seed weight at the early developmental stage in rapeseed.
基金supported by the National Basic Research Program of China(2015CB150201)the Natural Science Foundation of Chongqing(cstc2019jcyj-bshX0055,cstc2019jcyj-zdxmX0012cstc2020jcyj-msxmX0461)。
文摘Seed number per silique(SNPS)is one of seed yield components in rapeseed,but its genetic mechanism remains elusive.Here a double haploid(DH)population derived from a hybrid between female 6Q006with 35–40 SNPS and male 6W26 with 10–15 SNPS was investigated for SNPS in the year 2017,2018,2019 and 2021,and genotyped with Brassica 60K Illumina Infinium SNP array.An overlapping major QTL(qSNPS.C09)explaining 51.50%of phenotypic variance on average was narrowed to a 0.90 Mb region from 44.87 Mb to 45.77 Mb on chromosome C09 by BSA-seq.Subsequently,two DEGs in this interval were detected between extreme individuals in DH and F_2populations by transcriptome sequencing at7 and 14 days after pollination siliques.Of which,BnaC09g45400D encoded an adenine phosphoribosyltransferase 5(APT5)has a 48-bp InDel variation in the promoter of two parents.Candidate gene association analysis showed that this InDel variation was associated with SNPS in a nature population of rapeseed,where 54 accessions carrying the same haplotype as parent 6Q006 had higher SNPS than103 accessions carrying the same haplotype as parent 6W26.Collectively,the findings are helpful for rapeseed molecular breeding of SNPS,and provide new insight into the genetic and molecular mechanism of SNPS in rapeseed.
基金The National Major Research High Resolution Sea Ice Model Development Program of China under contract No.2018YFA0605903the National Natural Science Foundation of China under contract No.41776192the Fundamental Research Funds for the Central Universities under contract No.202165005。
文摘Over the past decades,sea ice in the polar regions has been significantly affecting local and even hemispheric climate through a positive ice albedo feedback mechanism.The role of fast ice,as opposed to drift ice,has not been well-studied due to its relatively small coverage over the earth.In this paper,the optical properties and surface energy balance of land fast ice in spring are studied using in situ observations in Barrow,Alaska.The results show that the albedo of the fast ice varied between 0.57 and 0.85 while the transmittance increased from 1.3×10-3 to 4.1×10-3 during the observation period.Snowfall and air temperature affected the albedo and absorbance of sea ice,but the transmittance had no obvious relationship with precipitation or snow cover.Net solar shortwave radiation contributes to the surface energy balance with a positive 99.2%of the incident flux,with sensible heat flux for the remaining 0.8%.Meanwhile,the ice surface loses energy through the net longwave radiation by 18.7%of the total emission,while the latent heat flux accounts for only 0.1%.Heat conduction is also an important factor in the overall energy budget of sea ice,contributing 81.2%of the energy loss.Results of the radiative transfer model reveal that the spectral transmittance of the fast ice is determined by the thickness of snow and sea ice as well as the amount of inclusions.As major inclusions,the ice biota and particulates have a significant influence on the magnitude and distribution of the spectral transmittance.Based on the radiative transfer model,concentrations of chlorophyll and particulate in the fast ice are estimated at 5.51 mg/m^(2)and 95.79 g/m^(2),which are typical values in the spring in Barrow.
基金Project supported by the Natural Science Foundation of Shandong Province,China(Grant Nos.ZR2009AM017 and ZR2013FM019)the National Postdoctoral Project of China(Grant Nos.200902574 and 20080441150)+1 种基金the Fund from the Educational Department of Shandong Province,China(Grant No.J06P14)the Opening Foundation of State Key Laboratory of Minning Disaster Prevention and Control Co-founded by Shandong Province and the Ministry of Science and Technology of China(Grant No.MDPC201602)
文摘Graphene’s optical absorption coefficient increases linearly with the number of layers making it more effective in the construction of optical tuning graphene-based devices. Refractive index(RI) is one of the important optical parameters of the graphene for accurately describing its optical characteristics and further applications. In view of the RI research of the multilayer graphene is lacking and existing measurement methods are complicated. Optical power tuning RI of multilayer graphene is investigated using a simple measurement and no temperature cross sensitivity all optical fiber sensing structure.Optical power tuning RI characteristics of multilayer graphene are studied by tuning the introducing broad band light power from 0.57 mW to 22.7 m W. Different thickness graphene coating shows different tuning efficiency. At 4.86-μm thickness,a 3.433-nm Bragg wavelength shift is obtained with 156.2-pm/mW wavelength versus optical power tuning sensitivity corresponding to 3.25×10~3 RI change and 0.154 URI/W(URI, unit of RI) RI optical power tuning efficiency.
基金The National Natural Science Foundation of China under contract No.42006037the Chinese Polar Environmental Comprehensive Investigation&Assessment Programs,Grant from the Scientific Research Fund of the Second Institute of Oceanography,MNR under contract No.JB904the National Key R&D Program of China under contract No.2019YFC1509102。
文摘Oceanic vertical mixing of the lower halocline water(LHW)in the Chukchi Borderland and Mendeleyev Ridge was studied based on in situ hydrographic and turbulent observations.The depth-averaged turbulent dissipation rate of LHW demonstrates a clear topographic dependence,with a mean value of 1.2×10^(-9) W/kg in the southwest of Canada Basin,1.5×10^(-9) W/kg in the Mendeleyev Abyssal Plain,2.4×10^(-9) W/kg on the Mendeleyev Ridge,and2.7×10^(-9) W/kg on the Chukchi Cap.Correspondingly,the mean depth-averaged vertical heat flux of the LHW is0.21 W/m^(2) in the southwest Canada Basin,0.30 W/m^(2) in the Mendeleyev Abyssal Plain,0.39 W/m^(2) on the Mendeleyev Ridge,and 0.46 W/m^(2) on the Chukchi Cap.However,in the presence of Pacific Winter Water,the upward heat released from Atlantic Water through the lower halocline can hardly contribute to the surface ocean.Further,the underlying mechanisms of diapycnal mixing in LHW—double diffusion and shear instability—was investigated.The mixing in LHW where double diffusion were observed is always relatively weaker,with corresponding dissipation rate ranging from 1.01×10^(-9) W/kg to 1.57×10^(-9) W/kg.The results also show a strong correlation between the depth-average dissipation rate and strain variance in the LHW,which indicates a close physical linkage between the turbulent mixing and internal wave activities.In addition,both surface wind forcing and semidiurnal tides significantly contribute to the turbulent mixing in the LHW.
基金Supported by Scientific Research Foundation of Hunan Provincial Department of Education(13B143)
文摘As a part of certain region,agricultural development has inevitable connection with regional urbanization,and they are highly heterogeneous and complementary in space. Their internal relationship is manifested as agricultural structure suits demand of urbanization development through adjustment in the process of urbanization,while urbanization is based on development of regional agriculture. From history of world agriculture and urbanization development,agriculture firstly provides primitive accumulation for urbanization development,and drives regional urbanization through promoting agricultural development. When urbanization develops to certain stage,urbanization accumulation increases and reaches the goal of " boosting urbanization through agriculture". If urbanization develops slowly,agricultural adjustment will lack motive force. On the contrary,weak agricultural development will lead to stagnation of urbanization. Therefore,adjustment of agricultural structure and urbanization development are interconnected,mutually promoted and restricted,and interdependent.
基金LHTD(20170003)and the Guangdong Laboratory of Artificial Intelligence and Digital Economy(SZ).
文摘Background In recent decades,unmanned aerial vehicles(UAVs)have developed rapidly and been widely applied in many domains,including photography,reconstruction,monitoring,and search and rescue.In such applications,one key issue is path and view planning,which tells UAVs exactly where to fly and how to search.Methods With specific consideration for three popular UAV applications(scene reconstruction,environment exploration,and aerial cinematography),we present a survey that should assist researchers in positioning and evaluating their works in the context of existing solutions.Results/Conclusions It should also help newcomers and practitioners in related fields quickly gain an overview of the vast literature.In addition to the current research status,we analyze and elaborate on advantages,disadvantages,and potential explorative trends for each application domain.
基金support from the National Natural Science Foundation of China(Nos.21971107,22071101,22271147).
文摘This work describes intermolecular acylfluorination of gem-difluoroenynes using acyl fluorides as both acyl source and fluorine source.Trifluoromethyl-substituted allenones or furans could be selectively achieved via phosphine and silver catalysis.These approaches exhibit high regioselectivity,atom econ-omy and broad functionality tolerance.
基金Youth Talent Support Program of Fujian Province(Eyas Plan of Fujian Province)National Natural Science Foundation of China(62175205)Natural Science Foundation of Fujian Province(2020J06009)。
文摘Surface acoustic wave(SAW)resonators based on lithium tantalate(LT,LiTaO_(3))wafers are crucial elements of mobile communication filters.The use of intrinsic LT wafers typically brings about low fabrication accuracy of SAW resonators due to strong UV reflection in the lithography process.This hinders their resonance frequency control seriously in industrial manufacture.LT doping and chemical reduction could be applied to decrease the UV reflection of LT wafers for high lithographic precision.However,conventional methods fail to provide a fast and nondestructive approach to identify the UV performance of standard single-side polished LT wafers for highprecision frequency control.Here,we propose a convenient on-line sensing scheme based on the colorimetry of reduced Fe-doped LT wafers and build up an automatic testing system for industrial applications.The levels of Fe doping and chemical reduction are evaluated by the lightness and color difference of LT-based wafers.The correlation between the wafer visible colorimetry and UV reflection is established to refine the lithography process and specifically manipulate the frequency performance of SAW resonators.Our study provides a powerful tool for the fabrication control of SAW resonators and will inspire more applications on sophisticated devices of mobile communication.
文摘The authors introduce the intactness-aware Mosaic data augmentation strategy,designed to tackle challenges such as low accuracy in detecting defects in insulation pull rods,limited timeliness in intelligent analysis,and the absence of a comprehensive database for information on insulation pull rod defects.The proposed strategy incorporates the YOLOv5s algorithm for detecting defects in insulation pull rods.Initially,the YOLOv5s network was constructed,and a dataset containing photos of insulation pull rods with white spots,fractures,impurities,and bubble flaws was compiled to capture images of defects.The research presented a data enhancement approach to improve the images and establish a dataset for insulation pull rod defects.The YOLOv5s algorithm was applied for both training and testing purposes.A comparative analysis was conducted to assess the detection performance of YOLOv5s against a conventional target detector for identifying defects in insulation pull rods.Furthermore,the utility of Mosaic's data augmentation technique,which incorporates intactness awareness,was evaluated to enhance the accuracy of identifying insulation pull rod defects.The research findings indicate that the YOLOv5s algorithm is employed for intelligent detection and precise localisation of flaws.The intactnessaware Mosaic data augmentation strategy significantly improves the accuracy of detecting faults in insulation pull rods.The YOLOv5s model used achieves a performance index mAP@0.5:0.95 of 0.563 on the test set,distinct from the training set data.With a threshold of 0.5,the mAP@0.5 score is 0.904,indicating a substantial improvement in both detection efficiency and accuracy compared to conventional target detection methods.Innovative approaches for identifying defects in insulation pull rods are introduced.
基金Project supported by the Major National Science and Technology Special Projects(No.2016ZX02301003-004-007)the Natural Science Foundation,China(No.61704046)the Hebei Natural Science Foundation Project(No.F2018202174)
文摘Benzotriazole(BTA), an anticorrosion agent of slurry, is the main organic pollutant remaining after CMP of multilayer copper wiring, and also the main removal object of post CMP cleaning. The adsorption of BTA onto the copper could form a dense Cu-BTA film, which makes the copper surface strongly passivated. According to this characteristic, quantitative analysis of BTA residue after cleaning is carried out by contact angle measurement and electrochemical measurement in this paper. A scanning electron microscope(SEM) with EDX was used to observe and analyze the BTA shape and elements. The efficiencies of organic alkali and inorganic alkali on the removal of BTA were studied. The corresponding reaction mechanism was also analyzed. The results show that the adsorption structure of Cu(I)-BTA cannot be destroyed in an alkaline environment with a pH less than 10; the effect of BTA removal by inorganic alkali is worse than that of the organic amine alkali with the coordination structure under the same pH environment; the FA/O Ⅱ chelating agent with the fraction of 200 ppm can effectively remove BTA residue on the surface of copper wafer.
基金Financial support for this project was provided by grants from the NIH(R41 AI134546–01)Medtronic-Bakken Endowed Chair for Engineering in Medicine to J.B.
文摘Detection of human immunodeficiency virus(HIV)p24 protein at a single pg/ml concentration in point-of-care(POC)settings is important because it can facilitate acute HIV infection diagnosis with a detection sensitivity approaching that of laboratory-based assays.However,the limit of detection(LOD)of lateral flow immunoassays(LFAs),the most prominent POC diagnostic platform,falls short of that of laboratory protein detection methods such as enzyme-linked immunosorbent assay(ELISA).Here,we report the development and optimization of a thermal contrast amplification(TCA)LFA that will allow ultrasensitive detection of 8 pg/ml p24 protein spiked into human serum at POC,approaching the LOD of a laboratory test.To achieve this aim,we pursued several innovations as follows:(a)defining a new quantitative figure of merit for LFA design based on the specific to nonspecific binding ratio(BR);(b)using different sizes and shapes of gold nanoparticles(GNPs)in the systematic optimization of TCA LFA designs;and(c)exploring new laser wavelengths and power regimes for TCA LFA designs.First,we optimized the blocking buffer for the membrane and running buffer by quantitatively measuring the BR using a TCA reader.The TCA reader interprets the thermal signal(i.e.,temperature)of GNPs within the membrane when irradiated by a laser at the plasmon resonance wavelength of the particle.This process results in higher detection and quantitation of GNPs than in traditional visual detection(i.e.,color intensity).Further,we investigated the effect of laser power(30,100,200 mW),GNP size and shape(30 and 100 nm gold spheres,150 nm gold-silica shells),and laser wavelength(532,800 nm).Applying these innovations to a new TCA LFA design,we demonstrated that 100nm spheres with a 100 mW 532 nm laser provided the best performance(i.e.,LOD=8pg/ml).This LOD is significantly better than that of the current colorimetric LFA and is in the range of the laboratory-based p24 ELISA.In summary,this TCA LFA for p24 protein shows promise for detecting acute HIV infection in POC settings.
基金the financial support from National Natural Science Foundation of China under Grant No.2018YFB2200400the National Science Foundation(JCYJ20180306172041577 NSF(61975041),JCYJ20180306172041577 NSF(11974092))the Shenzhen Fundamental research projects(JCYJ20180507183532343,JCYJ20180507184613841).
文摘Optical microcavities play a significant role in the study of classical and quantum chaos.To date,most experimental explorations of their internal wave dynamics have focused on the properties of their inputs and outputs,without directly interrogating the dynamics and the associated mode patterns inside.As a result,this key information is rarely retrieved with certainty,which significantly restricts the verification and understanding of the actual chaotic motion.Here we demonstrate a simple and robust approach to directly and rapidly map the internal mode patterns in chaotic microcavities.By introducing a local index perturbation through a pump laser,we report a spectral response of optical microcavities that is proportional to the internal field distribution.With this technique,chaotic modes with staggered mode spacings can be distinguished.Consequently,a complete chaos assisted tunneling(CAT)and its time-reversed process are experimentally verified in the optical domain with unprecedented certainty.