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PRRSV Nsp4基因突变株对β2M表达的影响
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作者 康磊 刘珂 +8 位作者 王建忠 尹才 邱亚峰 魏建超 李蓓蓓 邵东华 李宗杰 粟硕 马志永 《南京农业大学学报》 CAS CSCD 北大核心 2024年第3期507-514,共8页
[目的]本试验旨在探究猪繁殖与呼吸综合征病毒(PRRSV)Nsp4基因是否在抑制细胞β2M表达方面发挥关键性作用,以进一步研究PRRSV对抗原递呈的免疫抑制机制。[方法]建立PRRSV的反向遗传平台并成功拯救出PRRSV野生毒株SH2020。根据之前对Nsp... [目的]本试验旨在探究猪繁殖与呼吸综合征病毒(PRRSV)Nsp4基因是否在抑制细胞β2M表达方面发挥关键性作用,以进一步研究PRRSV对抗原递呈的免疫抑制机制。[方法]建立PRRSV的反向遗传平台并成功拯救出PRRSV野生毒株SH2020。根据之前对Nsp4基因不同结构域的研究以及氨基酸位点功能预测,在其DomainⅡ和DomainⅢ分别筛选出3个和5个不同的氨基酸位点,并在病毒感染性克隆质粒上对这些位点进行不同的氨基酸替换突变,再将构建好的感染性克隆质粒转染Marc-145细胞以拯救各个Nsp4突变PRRSV病毒,将拯救的PRRSV病毒以MOI=0.02感染PAM细胞,并通过RT-qPCR和Western blot验证PRRSV Nsp4基因是否能够在PAM细胞β2M表达方面发挥作用。[结果]成功拯救出PRRSV D2-5(Nsp4-A80L)突变毒株,并且在Marc-145细胞上的生长动力学特性与野生毒株SH2020相似;而Nsp4基因其余位点突变则会导致PRRSV生长受到显著抑制从而无法进行试验。Western blot和RT-qPCR试验结果证实PRRSV D2-5毒株能够显著促进β2M在Marc-145和PAM细胞的表达,而野生毒株SH2020则表现出了对β2M表达的抑制作用。[结论]Nsp4基因在PRRSV抑制细胞β2M表达方面发挥重要作用,并且Nsp4-A80L突变能够显著促进β2M的表达。 展开更多
关键词 PRRSV NSP4 Β2M 反向遗传平台 突变毒株
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宁夏贺兰山岩羊小反刍兽疫疫情来源分析
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作者 尹才 李知新 +12 位作者 李林 邸静 周海宁 张玉玲 白涛涛 王玉梅 赵源 林邱雄 吴亚文 马龙 李莉娟 王晓亮 曾巧英 《兽类学报》 CAS CSCD 北大核心 2024年第4期497-505,共9页
2018—2023年,宁夏贺兰山国家级自然保护区连续发现多起野生岩羊(Pseudois nayaur)不明原因死亡,通过解剖采样、实时荧光RT‑PCR检测,证实为小反刍兽疫病毒(PPRV)核酸阳性。使用Vero细胞分离出了部分毒株,采用RT‑PCR技术对宁夏地区病死... 2018—2023年,宁夏贺兰山国家级自然保护区连续发现多起野生岩羊(Pseudois nayaur)不明原因死亡,通过解剖采样、实时荧光RT‑PCR检测,证实为小反刍兽疫病毒(PPRV)核酸阳性。使用Vero细胞分离出了部分毒株,采用RT‑PCR技术对宁夏地区病死家养羊和野生动物病料组织扩增PPRV F基因的全长片段。使用DNASTAR Lasergene和MEGA X软件对岩羊PPRV F基因序列进行比对和分析,构建系统发育树分析其分子遗传特征。结果显示:宁夏地区不同年度家养羊和贺兰山岩羊感染的PPRV毒株F基因同源性均在99%以上,其中2023年毒株与2018年、2019年1月毒株同源性最高,为99.8%,与2014年、2016年宁夏家养羊PPR疫情毒株及China/XJYL/2013(KM091959.1)同源性为99.6%。遗传进化分析表明,宁夏野生岩羊及家养羊PPRV同源性较高,均属于基因Ⅳ系中亚分支。因此推测宁夏及周边省份流行的PPR疫情可能通过不同途径在贺兰山野生动物种群中扩散蔓延,需加强PPR等野生动物疫源疫病监测。 展开更多
关键词 宁夏贺兰山 岩羊 小反刍兽疫 分子遗传特征
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宁夏银北农村地区肉牛布鲁氏菌病监测与分析
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作者 贾会囡 刘剑辉 +2 位作者 王孝宗 尹才 李知新 《现代畜牧科技》 2024年第4期108-111,共4页
为探讨宁夏银北农村地区肉牛布鲁氏菌病的流行病学情况,试验在该地9个村庄开展,随机选择20个未接种布鲁氏菌疫苗的肉牛养殖场,每场采集20份全血,共400份样品,采用虎红平板凝集试验(RBPT)和竞争酶联免疫吸附试验(cELISA)检测肉牛血清抗... 为探讨宁夏银北农村地区肉牛布鲁氏菌病的流行病学情况,试验在该地9个村庄开展,随机选择20个未接种布鲁氏菌疫苗的肉牛养殖场,每场采集20份全血,共400份样品,采用虎红平板凝集试验(RBPT)和竞争酶联免疫吸附试验(cELISA)检测肉牛血清抗体情况,再采取实时荧光定量PCR(RT-qPCR)法对血清阳性样品进行检测,将qPCR结果为阳性的样品进一步采取AMOS-PCR法进行扩增分型。结果表明,RBPT和cELISA整体血清阳性率分别为为23.5%和27.5%,2种检测方法之间的Kappa值为0.816,一致性较好;RBPT和cELISA检测的群体平均阳性率分别为60%和65%;qPCR检测显示,只有5份血清样品布鲁氏菌阳性,经AMOS-PCR鉴定为牛种布鲁氏菌。说明牛种布鲁氏菌是引起该地区牛布鲁氏菌病的主要病原菌。通过探讨宁夏银北农村地区肉牛布鲁氏菌病的流行病学情况,发现该地肉牛布病具有较高的流行率,且检测发现感染布病的为牛种布鲁氏菌,为宁夏银北地区控制和预防布鲁氏菌的感染提供流行病学依据。 展开更多
关键词 农村地区 布鲁氏菌病 肉牛 监测 分析
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Caloric restriction-mimetics for the reduction of heart failure risk in aging heart: with consideration of gender-related differences 被引量:3
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作者 Lei Pang Xi Jiang +6 位作者 Xin Lian Jie Chen Er-Fei Song Lei-Gang Jin Zheng-Yuan Xia Hai-Chun Ma yin cai 《Military Medical Research》 SCIE CAS CSCD 2023年第1期113-133,共21页
The literature is full of claims regarding the consumption of polyphenol or polyamine-rich foods that offer some protection from developing cardiovascular disease(CVD). This is achieved by preventing cardiac hypertrop... The literature is full of claims regarding the consumption of polyphenol or polyamine-rich foods that offer some protection from developing cardiovascular disease(CVD). This is achieved by preventing cardiac hypertrophy and protecting blood vessels through improving the function of endothelium. However, do these interventions work in the aged human hearts? Cardiac aging is accompanied by an increase in left ventricular hypertrophy, along with diastolic and systolic dysfunction. It also confers significant cardiovascular risks for both sexes. The incidence and prevalence of CVD increase sharply at an earlier age in men than women. Furthermore, the patterns of heart failure differ between sexes, as do the lifetime risk factors. Do caloric restriction(CR)-mimetics, rich in polyphenol or polyamine, delay or reverse cardiac aging equally in both men and women? This review will discuss three areas:(1) mechanisms underlying age-related cardiac remodeling;(2) gender-related differences and potential mechanisms underlying diminished cardiac response in older men and women;(3) we select a few polyphenol or polyamine rich compounds as the CRmimetics, such as resveratrol, quercetin, curcumin, epigallocatechin gallate and spermidine, due to their capability to extend health-span and induce autophagy. We outline their abilities and issues on retarding aging in animal hearts and preventing CVD in humans. We discuss the confounding factors that should be considered for developing therapeutic strategies against cardiac aging in humans. 展开更多
关键词 Cardiovascular disease Cardiac aging Caloric restriction Gender difference Caloric restriction-mimetics Dietary compounds Clinical application
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Ionizable drug delivery systems for efficient and selective gene therapy 被引量:2
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作者 Yu-Qi Zhang Ran-Ran Guo +10 位作者 Yong-Hu Chen Tian-Cheng Li Wen-Zhen Du Rong-Wu Xiang Ji-Bin Guan Yu-Peng Li Yuan-Yu Huang Zhi-Qiang Yu yin cai Peng Zhang Gui-Xia Ling 《Military Medical Research》 SCIE CAS CSCD 2023年第6期818-847,共30页
Gene therapy has shown great potential to treat various diseases by repairing the abnormal gene function.However,a great challenge in bringing the nucleic acid formulations to the market is the safe and effective deli... Gene therapy has shown great potential to treat various diseases by repairing the abnormal gene function.However,a great challenge in bringing the nucleic acid formulations to the market is the safe and effective delivery to the specific tissues and cells.To be excited,the development of ionizable drug delivery systems(IDDSs)has promoted a great breakthrough as evidenced by the approval of the BNT162b2 vaccine for prevention of coronavirus disease 2019(COVID-19)in 2021.Compared with conventional cationic gene vectors,IDDSs can decrease the toxicity of carriers to cell membranes,and increase cellular uptake and endosomal escape of nucleic acids by their unique pH-responsive structures.Despite the progress,there remain necessary requirements for designing more efficient IDDSs for precise gene therapy.Herein,we systematically classify the IDDSs and summarize the characteristics and advantages of IDDSs in order to explore the underlying design mechanisms.The delivery mechanisms and therapeutic applications of IDDSs are comprehensively reviewed for the delivery of plasmid DNA(pDNA)and four kinds of RNA.In particular,organ selecting considerations and high-throughput screening are highlighted to explore efficiently multifunctional ionizable nanomaterials with superior gene delivery capacity.We anticipate providing references for researchers to rationally design more efficient and accurate targeted gene delivery systems in the future,and indicate ideas for developing next generation gene vectors. 展开更多
关键词 Ionizable nanomaterials Ionizable drug delivery systems(IDDSs) Nucleic acids Gene therapy
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国内外畜间布鲁氏菌病流行情况及防控
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作者 林邱雄 殷海燕 +3 位作者 尹才 王玉梅 王晓亮 李知新 《中国动物检疫》 CAS 2023年第10期67-73,共7页
布鲁氏菌病(简称布病)是由布鲁氏菌引起的一种人兽共患病,对养殖业和公共卫生安全造成了威胁。为了解全球畜间布病流行及分布情况,完善我国布病防控策略,收集了近几年国内外畜间布病调查研究数据,分析了畜间布病的流行特点。研究发现畜... 布鲁氏菌病(简称布病)是由布鲁氏菌引起的一种人兽共患病,对养殖业和公共卫生安全造成了威胁。为了解全球畜间布病流行及分布情况,完善我国布病防控策略,收集了近几年国内外畜间布病调查研究数据,分析了畜间布病的流行特点。研究发现畜间布病病例主要集中于发展中国家,我国畜间病例在多省份被发现且流行率总体呈现北高南低的特点。在参考国际布病净化经验的基础上,提出了我国布病防控策略,如加强监测及经费投入、养殖管理和引种检疫、宣传引导和防控培训、疫苗接种及阳性动物扑杀、布病净化等方面的工作,以期为我国畜间布病防控提供参考。 展开更多
关键词 布鲁氏菌病 畜间 流行病学 防控策略
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动物衣原体病诊断与防控
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作者 殷海燕 王晓亮 +5 位作者 邸静 尹才 周海宁 马龙 林邱雄 李知新 《现代畜牧科技》 2023年第11期96-99,共4页
动物衣原体病是由动物感染动物源性衣原体所致的一种常见人畜共患病。在自然界中不依赖人类的存在而存在,是一类典型的自然疫源性传染病。该病呈地方流行,常对养殖业和公共卫生造成很大危害。羊衣原体感染使少数发病羔羊前肢或后肢腕关... 动物衣原体病是由动物感染动物源性衣原体所致的一种常见人畜共患病。在自然界中不依赖人类的存在而存在,是一类典型的自然疫源性传染病。该病呈地方流行,常对养殖业和公共卫生造成很大危害。羊衣原体感染使少数发病羔羊前肢或后肢腕关节、跗关节出现关节炎,使母羊在最后1个月发生流产、死胎和产出病弱羔羊。该文重点探究动物衣原体病的诊断方法和防控方法,以期指导相关行业人员在深入了解动物衣原体病临床症状、病理变化等方面的基础上,通过使用多种方法进行诊断,并采取合适的治疗措施加以防控,以保障动物健康。 展开更多
关键词 动物衣原体病 衣原体 诊断 防治措施
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基于奇异谱分析的CORS大地高时序形变信息提取 被引量:5
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作者 李婉秋 王伟 +3 位作者 章传银 钟玉龙 尹财 朱钰 《大地测量与地球动力学》 CSCD 北大核心 2019年第8期825-831,共7页
为增强信号提取的信噪比,采用奇异谱分析(SSA)对测站大地高序列进行分解与重构,利用仿真实验确定嵌入维数与截断主分量个数的选取原则,进一步将SSA方法应用到CORS时序形变信息提取中。通过FFT分析发现,大地高时序以分解的季节性变化成... 为增强信号提取的信噪比,采用奇异谱分析(SSA)对测站大地高序列进行分解与重构,利用仿真实验确定嵌入维数与截断主分量个数的选取原则,进一步将SSA方法应用到CORS时序形变信息提取中。通过FFT分析发现,大地高时序以分解的季节性变化成分为主,重构后时序标准差明显减小,表明SSA方法可有效提高CORS大地高时序信噪比,起到降噪平滑作用。研究结果对地壳垂直形变特征表达及SSA应用拓展具有一定意义。 展开更多
关键词 CORS 大地高时序 信号提取 奇异谱分析 信噪比
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小反刍兽疫病毒F基因的序列分析 被引量:3
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作者 周海宁 尹才 +1 位作者 马龙 王晶钰 《动物医学进展》 北大核心 2018年第11期19-24,共6页
为弄清宁夏某羊场山羊感染的小反刍兽疫病毒(PPRV)F基因的分子特征,以宁夏某山羊养殖场采集的疑似小反刍兽疫病毒(PPRV)的病料为试验材料,提取病料RNA,扩增PPRV-F基因,阳性样品进行序列分析和系统进化树分析。同时,对该基因编码的F蛋白... 为弄清宁夏某羊场山羊感染的小反刍兽疫病毒(PPRV)F基因的分子特征,以宁夏某山羊养殖场采集的疑似小反刍兽疫病毒(PPRV)的病料为试验材料,提取病料RNA,扩增PPRV-F基因,阳性样品进行序列分析和系统进化树分析。同时,对该基因编码的F蛋白进行生物信息学分析,预测该蛋白的结构及理化特性。结果表明,成功扩增了PPRV-F基因,与预期大小相符,经测序及序列比对,证实获得病料为小反刍兽疫病毒(PPRV)阳性;系统进化分析表明,宁夏鉴定的PPRV与中国其他省份报道的8个毒株及印度、蒙古等国报道的7个毒株同属谱系Ⅳ;生物信息学分析显示,该蛋白为疏水蛋白,有信号肽、跨膜区。通过对宁夏分离病料的PPRV-F基因分析,提示宁夏感染的PPRV与中国其他省份分离的PPRV毒株同源;同时,对PPRV-F基因编码的F蛋白的生物信息学分析,为进一步研究该蛋白的生物学功能,研制基因工程疫苗奠定基础。 展开更多
关键词 小反刍兽疫病毒 F基因 克隆 序列分析
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日粮添加绞股蓝提取物对断奶仔猪生长性能、血液生化、免疫力及抗氧化功能的影响 被引量:2
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作者 邸静 魏彦明 +4 位作者 安泓霏 李知新 尹才 刘岩 赵源 《中国饲料》 北大核心 2021年第21期60-65,共6页
为研究日粮中添加不同水平的绞股蓝提取物对断奶仔猪生长性能、血液生化指标、免疫力及抗氧化功能的影响,试验选取21日龄体重相近的“杜×长×大”仔猪250头,随机分为5个处理组,每组5个重复,每个重复10头仔猪。对照组饲喂玉米-... 为研究日粮中添加不同水平的绞股蓝提取物对断奶仔猪生长性能、血液生化指标、免疫力及抗氧化功能的影响,试验选取21日龄体重相近的“杜×长×大”仔猪250头,随机分为5个处理组,每组5个重复,每个重复10头仔猪。对照组饲喂玉米-豆粕型基础日粮,其余4组在基础日粮中分别添加0.5%、1.0%、1.5%、2.0%的绞股蓝提取物。预饲期7 d,正式试验期28 d。结果表明:与对照组相比,1.0%和1.5%绞股蓝提取物组的仔猪试验末重和平均日增重显著提高8.80%、8.16%和17.65%、17.23%(P<0.05);绞股蓝提取物组料重比分别显著降低8.12%、13.20%、13.20%、7.61%(P<0.05),断奶仔猪的腹泻率分别显著降低23.52%、48.52%、59.96%、44.92%(P<0.05)。1.0%和1.5%绞股蓝提取物组的仔猪总胆固醇显著降低15.92%和13.49%(P<0.05);1.0%、1.5%和2.0%绞股蓝提取物组甘油三酯分别显著降低25.00%、30.36%、28.57%(P<0.05);1.0%和1.5%绞股蓝提取物组尿素氮分别显著降低24.91%和25.66%(P<0.05);1.0%、1.5%、2.0%绞股蓝提取物组IgA分别显著提高25.66%、23.89%和28.32%(P<0.05);1.0%和1.5%绞股蓝提取物组IgG分别显著提高52.69%和53.08%(P<0.05);绞股蓝提取物组总抗氧化能力(T-AOC)分别显著提高11.55%、29.35%、25.63%和12.22%(P<0.05);1.0%和1.5%绞股蓝提取物组超氧化物歧化酶(SOD)分别显著提高32.76%和30.70%(P<0.05);1.0%和1.5%绞股蓝提取物组丙二醛(MDA)分别显著降低31.47%和31.20%(P<0.05)。本试验条件下,断奶仔猪日粮中添加1.0%~1.5%剂量的绞股蓝提取物效果较好。 展开更多
关键词 绞股蓝提取物 生长性能 血液生化 免疫力 抗氧化 断奶仔猪
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宁夏鸡传染性支气管炎病原学检测及N基因序列分析 被引量:2
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作者 闵泽 周海宁 +4 位作者 马龙 尹才 马林 魏凡华 李知新 《中国动物检疫》 CAS 2022年第4期36-40,共5页
为了解宁夏地区鸡传染性支气管炎(infectious bronchitis,IB)流行情况,分析鸡传染性支气管炎病毒(infectious bronchitis virus,IBV)流行毒株的优势基因型,2021年9—11月,在宁夏8个县(区)部分规模化禽场和散养户,随机采集鸡咽喉/泄殖腔... 为了解宁夏地区鸡传染性支气管炎(infectious bronchitis,IB)流行情况,分析鸡传染性支气管炎病毒(infectious bronchitis virus,IBV)流行毒株的优势基因型,2021年9—11月,在宁夏8个县(区)部分规模化禽场和散养户,随机采集鸡咽喉/泄殖腔拭子646份,采用RT-PCR方法进行IBV核酸检测,对阳性样品进行病毒分离,对分离毒株的N基因进行遗传进化分析。结果显示:在646份样品中检出阳性88份,总阳性率为13.62%,其中西吉县、利通区阳性率较高,分别为36.00%、32.29%,70日龄蛋鸡阳性率高于120日龄以上鸡群。本试验成功分离出1株IBV毒株。基因分析显示,分离株属于QX型,与我国山东SDIB702/2012株亲缘关系最近,同源性为97.55%,与QX代表株P100株同源性为93.31%,与我国常用疫苗血清型H120、M41型毒株的同源性分别为86.67%、86.14%。结果表明:宁夏地区存在一定的IB流行,且呈现一定的地区、季节暴发特征,低日龄鸡群感染较严重,需要加强防控;流行的IBV毒株属于我国流行的优势基因型QX型,与我国常用的Mass型疫苗毒株同源性较低,现有疫苗可能难以对其产生有效的免疫保护作用。本次研究初步明确了宁夏地区蛋鸡群IB流行情况和流行毒株的遗传特点,可为宁夏地区的IB防控提供参考。 展开更多
关键词 鸡传染性支气管炎 流行病学调查 病毒分离 基因分析
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腹部外科手术切口裂开的原因分析及护理体会 被引量:4
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作者 尹彩 李雅莉 +1 位作者 梁梅燕 梁可为 《中外医学研究》 2021年第3期90-92,共3页
目的:分析腹部外科手术患者发生切口裂开的原因及护理干预措施。方法:选取2017年4月-2019年11月笔者所在医院腹部外科手术切口裂开的50例患者作为研究对象。根据入院时间先后顺序将其分为对照组和观察组,每组25例。对照组采取常规护理干... 目的:分析腹部外科手术患者发生切口裂开的原因及护理干预措施。方法:选取2017年4月-2019年11月笔者所在医院腹部外科手术切口裂开的50例患者作为研究对象。根据入院时间先后顺序将其分为对照组和观察组,每组25例。对照组采取常规护理干预,观察组采取综合护理干预。比较两组切口愈合质量、切口愈合时间、住院总时间、护理前后疼痛评分及正面情绪评分。结果:50例患者切口裂开原因中,切口感染26例,占52%;肥胖患者切口脂肪液化8例,占16%;贫血、低蛋白血症7例,占14%;糖尿病患者5例,占10%;腹压升高4例,占8%。观察组切口愈合优良率明显优于对照组,差异有统计学意义(P<0.05)。观察组切口愈合时间、住院时间均优于对照组,差异有统计学意义(P<0.05)。观察组疼痛评分明显低于对照组,正面情绪评分高于对照组,差异有统计学意义(P<0.05)。结论:对于接受腹部外科手术治疗患者,导致切口开裂的原因较为复杂,对患者展开综合护理可改善患者预后。 展开更多
关键词 腹部外科手术 切口裂开 护理
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2017年宁夏地区牛流行热血清流行病学调查 被引量:3
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作者 尹才 高闪电 +5 位作者 马龙 王玉梅 张成莲 周海宁 李知新 王晓亮 《中国奶牛》 2018年第12期35-37,共3页
为了解宁夏地区牛流行热的流行和分布情况,科学制定牛流行热防控措施,本研究于2017年5月和10月份在宁夏5个市22个县(市、区)63个场户采集牛血清共1 260份,使用间接ELISA方法进行牛流行热病毒抗体检测。试验结果显示,宁夏地区牛流行热病... 为了解宁夏地区牛流行热的流行和分布情况,科学制定牛流行热防控措施,本研究于2017年5月和10月份在宁夏5个市22个县(市、区)63个场户采集牛血清共1 260份,使用间接ELISA方法进行牛流行热病毒抗体检测。试验结果显示,宁夏地区牛流行热病毒抗体阳性样品有65份,阳性率为5.16%,散养户阳性率显著高于规模场,肉牛抗体阳性率高于奶牛。本次调查结果表明宁夏地区牛群中存在牛流行热病毒感染,但感染率较低。 展开更多
关键词 宁夏 牛流行热 血清流行病学调查
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Propofol postconditioning ameliorates hypoxia/reoxygenation induced H9c2 cell apoptosis and autophagy via upregulating forkhead transcription factors under hyperglycemia 被引量:9
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作者 Rong-Hui Han He-Meng Huang +9 位作者 Hong Han Hao Chen Fei Zeng Xiang Xie Dan-Yong Liu yin cai Liang-Qing Zhang Xin Liu Zheng-Yuan Xia Jing Tang 《Military Medical Research》 SCIE CSCD 2022年第3期286-302,共17页
Background:Administration of propofol,an intravenous anesthetic with antioxidant property,immediately at the onset of post-ischemic reperfusion(propofol postconditioning,P-PostC) has been shown to confer cardioprotect... Background:Administration of propofol,an intravenous anesthetic with antioxidant property,immediately at the onset of post-ischemic reperfusion(propofol postconditioning,P-PostC) has been shown to confer cardioprotection against ischemia–reperfusion(I/R) injury,while the underlying mechanism remains incompletely understood.The forkhead box O(FoxO) transcription factors are reported to play critical roles in activating cardiomyocyte survival signaling throughout the process of cellular injuries induced by oxidative stress and are also involved in hypoxic postconditioning mediated neuroprotection,however,the role of FoxO in postconditioning mediated protection in the heart and in particular in high glucose condition is unknown.Methods:Rat heart-derived H9c2 cells were exposed to high glucose(HG) for 48 h,then subjected to hypoxia/reoxygenation(H/R,composed of 8 h of hypoxia followed by 12 h of reoxygenation) in the absence or presence of postconditioning with various concentrations of propofol(P-PostC) at the onset of reoxygenation.After having identified the optical concentration of propofol,H9c2 cells were subjected to H/R and P-PostC in the absence or presence of FoxO1 or FoxO3a gene silencing to explore their roles in P-PostC mediated protection against apoptotic and autophagic cell deaths under hyperglycemia.Results:The results showed that HG with or without H/R decreased cell viability,increased lactate dehydrogenase(LDH) leakage and the production of reactive oxygen species(ROS) in H9c2 cells,all of which were significantly reversed by propofol(P-PostC),especially at the concentration of 25 μmol/L(P25)(P<0.05,NC vs.HG;HG vs.HG+HR;HG+HR+P12.5 or HG+HR+P25 or HG+HR+P50 vs.HG+HR).Moreover,we found that propofol(P25) decreased H9c2 cells apoptosis and autophagy that were concomitant with increased FoxO1 and FoxO3a expression(P<0.05,HG+HR+P25 vs.HG+HR).The protective effects of propofol(P25) against H/R injury were reversed by silencing FoxO1 or FoxO3a(P<0.05,HG+HR+P25 vs.HG+HR+P25+siRNA-1 or HG+HR+P25+siRNA-5).Conclusions:It is concluded that propofol postconditioning attenuated H9c2 cardiac cells apoptosis and autophagy induced by H/R injury through upregulating FoxO1 and FoxO3a under hyperglycemia. 展开更多
关键词 Hypoxia/reoxygenation injury HYPERGLYCEMIA High glucose Propofol postconditioning Apoptosis AUTOPHAGY Forkhead box O
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Mitochondrial carnitine palmitoyl transferase-Ⅱ inactivity aggravates lipid accumulation in rat hepatocarcinogenesis 被引量:8
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作者 Juan-Juan Gu Min Yao +5 位作者 Jie Yang yin cai Wen-Jie Zheng Li Wang Deng-Bing Yao Deng-Fu Yao 《World Journal of Gastroenterology》 SCIE CAS 2017年第2期256-264,共9页
AIM To investigate the dynamic alteration of mitochondrial carnitine palmitoyl transferase Ⅱ(CPT-Ⅱ) expression during malignant transformation of rat hepatocytes.METHODS Sprague-Dawley male rats were fed with normal... AIM To investigate the dynamic alteration of mitochondrial carnitine palmitoyl transferase Ⅱ(CPT-Ⅱ) expression during malignant transformation of rat hepatocytes.METHODS Sprague-Dawley male rats were fed with normal, high fat(HF), and HF containing 2-fluorenylacetamide(2-FAA) diet, respectively. According to the Hematoxylin and Eosin staining of livers, rats were divided into control, fatty liver, degeneration, pre-cancerous, and cancerous groups. Liver lipids were dyed with Oil Red O, CPT-Ⅱ alterations were analyzed by immunohistochemistry, and compared with CPT-Ⅱ specific concentration(μg/mg protein). Levels of total cholesterol(Tch), triglyceride(TG), and aminotransferases [alanine aminotransferase(ALT), aspartate aminotransferase(AST)] were determined by the routine methods.RESULTS After intake of HF and/or HF+2-FAA diets, the rat livers showed mass lipid accumulation. The lipid level in the control group was significantly lower than that in other groups. The changes of serum TG and Tch levels were abnormally increasing, 2-3 times more than those in the controls(P < 0.05). During the rat liver morphological changes from normal to cancer development process with hepatocyte injury, serum AST and ALT levels were significantly higher(4-8 times, P < 0.05) than those in the control group. The specific concentration of CPT-Ⅱ in liver tissues progressively decreased during hepatocyte malignant transformation, with the lowest CPT-Ⅱ levels in the cancer group than in any of the other groups(P < 0.05).CONCLUSION Low CPT-Ⅱ expression might lead to abnormal hepatic lipid accumulation, which should promote the malignant transformation of hepatocytes. 展开更多
关键词 Fatty liver Carnitine palmitoyl transferaseⅡ Malignant transformation of hepatocytes Dynamic expression Rat model
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Oncogenic Wnt3a expression as an estimable prognostic marker for hepatocellular carcinoma 被引量:5
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作者 Liu-Hong Pan Min Yao +4 位作者 yin cai Juan-Juan Gu Xu-Li Yang Li Wang Deng-Fu Yao 《World Journal of Gastroenterology》 SCIE CAS 2016年第14期3829-3836,共8页
AIM: To investigate member 3a of Wingless-type MMTV integration site family (Wnt3a) expression in cancerous and surrounding tissues and the relationship between clinicopathologic features of hepatocellular carcinoma (... AIM: To investigate member 3a of Wingless-type MMTV integration site family (Wnt3a) expression in cancerous and surrounding tissues and the relationship between clinicopathologic features of hepatocellular carcinoma (HCC) and Wnt3a expression.METHODS: Wnt3a expression and cellular distribution and clinicopathologic characteristics in cancerous tissue and matched surrounding tissues were analyzed in 80 HCC patients from January 2006 to August 2008 by tissue microarrays and immunohistochemistry. The overall and disease-free survival rates were estimated using the Kaplan-Meier method and compared with the log-rank test. The prognostic analysis was carried out with univariate and multivariate Cox regressions models.RESULTS: The incidence of oncogenic Wnt3a expression in the cancerous group was up to 96.25% (77 of 80), which was significantly higher (&#x003c7;<sup>2</sup> = 48.818, P &#x0003c; 0.001) than that in the surrounding group (46.25%, 37 of 80). Brown Wnt3a staining gradually increased with clinical staging that showed very strong staining in advanced HCC. The clinicopathologic features of high Wnt3a expression in HCC were related to poorly-differentiated grade (&#x003c7;<sup>2</sup> = 20.211, P &#x0003c; 0.001), liver cirrhosis (&#x003c7;<sup>2</sup> = 8.467, P &#x0003c; 0.004), hepatitis B virus (HBV) infection (&#x003c7;<sup>2</sup> = 12.957, P &#x0003c; 0.001), higher tumor-node-metastasis stage (&#x003c7;<sup>2</sup> = 22.960, P &#x0003c; 0.001), and 5-year survival rate (&#x003c7;<sup>2</sup> = 15.469, P &#x0003c; 0.001).CONCLUSION: Oncogenic Wnt3a expression associated with HBV infection and cirrhotic liver might be an independent prognostic factor for HCC. 展开更多
关键词 Hepatocellular carcinoma PROGNOSIS WNT3A Wnt/β -catenin signal Tissue microarrays IMMUNOHISTOCHEMISTRY
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优质护理干预对采血后皮下血肿形成的预防效果观察 被引量:1
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作者 尹彩 李雅莉 +1 位作者 梁梅燕 梁可为 《临床医学工程》 2021年第4期519-520,共2页
目的探讨优质护理干预对采血后皮下血肿形成的预防效果。方法选取2018年1月至2018年12月于我院接受采血的患者100例作为对照组,予以常规护理;选取2019年1月至2019年12月于我院接受采血的患者100例作为观察组,予以优质护理干预。比较两... 目的探讨优质护理干预对采血后皮下血肿形成的预防效果。方法选取2018年1月至2018年12月于我院接受采血的患者100例作为对照组,予以常规护理;选取2019年1月至2019年12月于我院接受采血的患者100例作为观察组,予以优质护理干预。比较两组的采血后皮下血肿发生率以及护理满意度。结果观察组的采血后皮下血肿发生率为1.00%,显著低于对照组的8.00%(P <0.05)。观察组的护理满意度为98.00%,显著高于对照组的87.00%(P <0.05)。结论优质护理干预能够有效降低患者采血后皮下血肿发生率,提高护理满意度,值得临床应用。 展开更多
关键词 优质护理干预 采血 皮下血肿 效果
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Reversal of multidrug resistance of hepatocellular carcinoma cells by metformin through inhibiting NF-κB gene transcription 被引量:6
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作者 Wei Wu Jun-Ling Yang +7 位作者 Yi-Lang Wang Han Wang Min Yao Li Wang Juan-Juan Gu yin cai Yun Shi Deng-Fu Yao 《World Journal of Hepatology》 CAS 2016年第23期985-993,共9页
AIM: To interfere with the activation of nuclear factor-κB(NF-κB) with metformin and explore its effect in reversing multidrug resistance(MDR) of hepatocellular carcinoma(HCC) cells.METHODS: Expression of P-glycopro... AIM: To interfere with the activation of nuclear factor-κB(NF-κB) with metformin and explore its effect in reversing multidrug resistance(MDR) of hepatocellular carcinoma(HCC) cells.METHODS: Expression of P-glycoprotein(P-gp) and NF-κB in human HepG 2 or HepG 2/adriamycin(ADM) cells treated with pC MV-NF-κB-small interference RNA(siR NA) with or without metformin, was analyzed by Western blot or fluorescence quantitative PCR. Cell viability was tested by CCK-8 assay. Cell cycle and apoptosis were measured by flow cytometry and Annexin-V-PE/7-AnnexinV apoptosis detection double staining assay, respectively. RESULTS: P-gp overexpression in HepG 2 and HepG 2/ADM cells was closely related to mdr1 mR NA(3.310 ± 0.154) and NF-κB mR NA(2.580 ± 0.040) expression. NF-κB gene transcription was inhibited by specific siR NA with significant down-regulation of P-gp and enhanced HCC cell chemosensitivity to doxorubicin. After pretreatment with metformin, Hep G2/ADM cells were sensitized to doxorubicin and P-gp was decreased through the NF-κB signaling pathway. The synergistic effect of metformin and NF-κB siR NA were found in HepG 2/ADM cells with regard to proliferation inhibition, cell cycle arrest and inducing cell apoptosis. CONCLUSION: Metformin via silencing NF-κB signaling could effectively reverse MDR of HCC cells by downregulating MDR1/P-gp expression. 展开更多
关键词 METFORMIN REVERSAL MULTIDRUG resistance HEPATOCELLULAR carcinoma
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Chemosensitization of HepG2 cells by suppression of NF-κB/p65 gene transcription with specific-si RNA 被引量:4
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作者 Yun Shi Si-Ye Wang +6 位作者 Min Yao Wen-Li Sai Wei Wu Jun-Ling Yang yin cai Wen-Jie Zheng Deng-Fu Yao 《World Journal of Gastroenterology》 SCIE CAS 2015年第45期12814-12821,共8页
AIM: To investigate small interfering RNA(si RNA)-mediated inhibition of nuclear factor-kappa B(NF-κB) activation and multidrug-resistant(MDR) phenotype formation in human Hep G2 cells. METHODS: Total RNA was extract... AIM: To investigate small interfering RNA(si RNA)-mediated inhibition of nuclear factor-kappa B(NF-κB) activation and multidrug-resistant(MDR) phenotype formation in human Hep G2 cells. METHODS: Total RNA was extracted from human Hep G2 or LO2 cells. NF-κB/p65 m RNA was amplified by nested reverse transcription polymerase chain reaction and confirmed by sequencing. NF-κB/p65 was analyzed by immunohistochemistry. Specific-si RNA was transfected to Hep G2 cells to knock down NF-κB/p65 expression. The effects on cell proliferation, survival, and apoptosis were assessed, and the level of NF-κB/p65 or P-glycoprotein(P-gp) was quantitatively analyzed by enzyme-linked immunosorbent assay.RESULTS: Hep G2 cells express NF-κB/p65 and express relatively less phosphorylated p65(P-p65) and little P-gp. After treatment of Hep G2 cells with different doses of doxorubicin, the expression of NF-κB/p65, P-p65, and especially P-gp were dose-dependently upregulated. After Hep G2 cells were transfected with NF-κB/p65 si RNA(100 nmol/L), the expression of NF-κB/p65, P-p65, and P-gp were downregulatedsignificantly and dose-dependently. The viability of Hep G2 cells was decreased to 23% in the combination NF-κB/p65 si RNA(100 nmol/L) and doxorubicin(0.5 μmol/L) group and 47% in the doxorubicin(0.5 μmol/L) group(t = 7.043, P < 0.001). CONCLUSION: Knockdown of NF-κB/p65 with si RNA is an effective strategy for inhibiting Hep G2 cell growth by downregulating P-gp expression associated chemosensitization and apoptosis induction. 展开更多
关键词 HEPATOCELLULAR CARCINOMA Nuclear factorκB Multidru
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基于非洲猪瘟病毒标准物质的荧光PCR检测试剂盒初步评价 被引量:4
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作者 马龙 尹才 +3 位作者 李莉 李杰 王晓亮 李知新 《中国动物检疫》 CAS 2021年第4期108-111,共4页
为探究非洲猪瘟病毒(African swine fever virus,ASFV)标准物质作为试剂盒评价体系的可行性,比较了市场上5种主流品牌ASFV荧光PCR检测试剂盒的检测性能。使用ASFV P72基因核酸标准物质作为模板,根据5种试剂盒说明书分别进行相应的荧光定... 为探究非洲猪瘟病毒(African swine fever virus,ASFV)标准物质作为试剂盒评价体系的可行性,比较了市场上5种主流品牌ASFV荧光PCR检测试剂盒的检测性能。使用ASFV P72基因核酸标准物质作为模板,根据5种试剂盒说明书分别进行相应的荧光定量PCR检测,结合扩增曲线、Ct值,分析不同试剂盒的敏感性、可重复性以及所需反应时间。结果显示:5种试剂盒的阴性、阳性对照均成立,最低检测限均为5.9×10^(-1)拷贝/μL;4个厂家的试剂盒线性关系R2>0.98,其中最优的R^(2)=0.994,离散度最小;各试剂盒的实际反应耗时与理论反应耗时均有一定差异(0.20~0.96 h)。结果表明,各生产厂家使用P72基因作为靶基因研制的ASFV荧光PCR检测试剂盒都可以使用ASFV标准物质作为评价体系,来评判试剂盒的检测性能。本试验为各实验室不同样品检测的ASFV荧光PCR检测试剂盒选择提供了一种可用的评价方法。 展开更多
关键词 非洲猪瘟病毒 标准物质 荧光PCR检测试剂盒 比对
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