The chlorophyll content has a direct effect on photosynthesis of crops.In order to explore a quick and convenient method for estimating the chlorophyll content of Brassica napus and facilitate efficient crop monitorin...The chlorophyll content has a direct effect on photosynthesis of crops.In order to explore a quick and convenient method for estimating the chlorophyll content of Brassica napus and facilitate efficient crop monitoring,we measured the actual value of chlorophyll with a SPAD-502 chlorophyll detector,and collected aerial images of B.napus with an unmanned aerial vehicle(UAV)carrying a RGB camera in this study.The total number of 270samples collected images were divided into regions according to the planting conditions of different B.napus varieties in the field.Then,according to the empirical formula,there were 36 colors’characteristic parameters calculated and combined.To estimate the chlorophyll content of rape,189 samples were included in the modeling set,while the other 81 samples were enrolled in the validation set for testing the accuracy of this model.After the combination of R(red),G(green)and B(blue)color channels,the results showed that the color characteristics B/(R+G),b,B/G,(G-B)/(G+B),g-b were highly connected with the measured value of chlorophyll SPAD,and the correlation coefficient between the combination based on B/(R+G)and SPAD value was 0.747.With R2=0.805,RMSE=3.343,and RE=6.84%,the regression model created using random forest had superior outcomes,according to the model comparison.This study offers a new method for quickly estimating the amount of chlorophyll in rapeseed and a workable reference for crop monitoring using the UAV platform.展开更多
Background:Nuclear receptor binding SET domain protein-3(NSD3)is a histone lysine methyltransferase and a crucial regulator of carcinogenesis in several cancers.We aimed to investigate the prognostic value and potenti...Background:Nuclear receptor binding SET domain protein-3(NSD3)is a histone lysine methyltransferase and a crucial regulator of carcinogenesis in several cancers.We aimed to investigate the prognostic value and potential function of NSD3 in 33 types of human cancer.Methods:The data were obtained from The Cancer Genome Atlas.Kaplan-Meier analysis,CIBERSORT,gene set enrichment analysis,and gene set variation analysis were performed.The expression of NSD3 was measured using quantitative real-time polymerase chain reaction and western blot.Results:The expression of NSD3 was altered in pan-cancer samples.Patients with higher levels of NDS3 generally had shorter overall survival and disease-specific survival.Levels of NSD3 were positively correlated with DNA copy number variation(CNV)in pan-cancer.NSD3 expression was also associated with tumor mutation burden and microsatellite instability.The levels of immune-cell infiltration differed significantly between high and low NSD3 expression.NSD3 negatively correlated with levels of CD8+T cells.Functional enrichment analysis showed that while NSD3 expression was positively associated with several immune cell-related and histone methylation-related pathways,it was negatively correlated with cell metabolism-related,drug transport-related,and drug metabolismrelated pathways.NSD3 levels in the cell lines tested were significantly different.In U251 and NCI-H23 cells,silencing NSD3 inhibited cell proliferation and promoted apoptosis.Conclusions:NSD3 expression was changed in pan-cancer samples that was also verified in cell lines.NSD3 was associated with CNV and immune-cell infiltration.A poor prognosis was predicted in patients with high expression of NSD3.NSD3 might hence be a potential marker for predicting tumor prognosis.展开更多
People continue to recognize the important role of Chinese medicine in the treatment of diseases,and there is an urgent need to clarify the mechanism of Chinese medicine.Because of the multi-target and multi-component...People continue to recognize the important role of Chinese medicine in the treatment of diseases,and there is an urgent need to clarify the mechanism of Chinese medicine.Because of the multi-target and multi-component characteristics of traditional Chinese medicine,it is difficult to elaborate from a single perspective.As a high-quality animal model,Caenorhabditis elegans with the characteristics of small size and fast reproduction,has been widely used in traditional Chinese medicine activity experiments.In this article,Caenorhabditis elegans,as model organisms used to screen the active components of traditional Chinese medicine were reviewed.展开更多
Design parameters at different scales in the pre-design phase could significantly impact both building energy consumption and photovoltaic(PV)power generation potential.However,existing studies often overlook the syne...Design parameters at different scales in the pre-design phase could significantly impact both building energy consumption and photovoltaic(PV)power generation potential.However,existing studies often overlook the synergistic effects of design parameters across multiple scales(block-building-facade scales)when evaluating these aspects.This paper aims to propose a workflow for the assessing building energy consumption and PV power generation potential of office blocks applicable in the pre-schematic design phase considering the synergistic influence of multi-scale design parameters,using building typology and parametric modelling approach.The study proposed a multi-scale design parameter classification system combined with parametric modelling.The study investigated 80 office blocks in Wuhan as the study case,which were classified into array type and enclosed type.Correlation analysis and multiple regression equations were used to quantify the single versus synergistic effects of different scale design parameters.Results suggest that focusing solely on a single scale during the pre-design stage is typically inadequate for understanding building energy potential.In contrast,multi-scale synergistic analysis boosts energy use intensity(EUI)by 7.56%and net energy use intensity(NEUI)by 33.96%.Under multi-scale synergistic conditions,the EUI of array type is more influenced by the building design parameters,while the NEUI is effected by the balance of multi-scales design parameters.While the EUI of enclosed types exhibit balanced effects across multi-scale design parameters,with NEUI results aligning closely with PV power generation potential.Multiple regression equations highlight building density and shape factor as key influencers for both array and enclosure layouts.This study offers designers a flexible and scalable workflow for evaluating building energy consumption and PV power generation potential in the pre-design phase.The findings can guide nearly-zero energy urban block planning to achieve a balance between energy supply and demand.展开更多
A new rhodamine B derivative T1 has been rationally synthesized and displayed selective Pd（Ⅱ）-amplified absorbance and fluorescence emission above 540 nm in methanol-water. Upon the addition of Pd（Ⅱ）, the spirol...A new rhodamine B derivative T1 has been rationally synthesized and displayed selective Pd（Ⅱ）-amplified absorbance and fluorescence emission above 540 nm in methanol-water. Upon the addition of Pd（Ⅱ）, the spirolactam ring was unfolded and a 1：1 metal-ligand complex formed, which can be used for ＇＇naked-eyes＂ detection. In addition, fluorescence imaging experiments of Pd^（2＋） in HepG2 living cells showed its valuable application in biological systems.展开更多
Although carbon monoxide(CO)-based treatments have demonstrated the high cancer efficacy by promoting mitochondrial damage and core-region penetrating ability,the efficiency was often compromised by protective autopha...Although carbon monoxide(CO)-based treatments have demonstrated the high cancer efficacy by promoting mitochondrial damage and core-region penetrating ability,the efficiency was often compromised by protective autophagy(mitophagy).Herein,cannabidiol(CBD)is integrated into biomimetic carbon monoxide nanocomplexes(HMPOC@M)to address this issue by inducing excessive autophagy.The biomimetic membrane not only prevents premature drugs leakage,but also prolongs blood circulation for tumor enrichment.After entering the acidic tumor microenvironment,carbon monoxide(CO)donors are stimulated by hydrogen oxide(H_(2)O_(2))to disintegrate into CO and Mn^(2+).The comprehensive effect of CO/Mn^(2+)and CBD can induce ROS-mediated cell apoptosis.In addition,HMPOC@Mmediated excessive autophagy can promote cancer cell death by increasing autophagic flux via classⅢPI3K/BECN1 complex activation and blocking autolysosome degradation via LAMP1 downregulation.Furthermore,in vivo experiments showed that HMPOC@M+laser strongly inhibited tumor growth and attenuated liver and lung metastases by downregulating VEGF and MMP9 proteins.This strategy may highlight the pro-death role of excessive autophagy in TNBC treatment,providing a novel yet versatile avenue to enhance the efficacy of CO treatments.Importantly,this work also indicated the applicability of CBD for triple-negative breast cancer(TNBC)therapy through excessive autophagy.展开更多
To the Editor： Several kinds of malignant tumors show perineural invasion （PNI）, which can cause locoregional recurrence and reduce the likelihood of survival after surgery. Our previous study has shown that PNI in...To the Editor： Several kinds of malignant tumors show perineural invasion （PNI）, which can cause locoregional recurrence and reduce the likelihood of survival after surgery. Our previous study has shown that PNI in cervical cancer is associated with other pathology risk features, and other studies have shown that it is a predictor of poor prognosis. These findings highlight the importance of clarifying the molecular mechanism of PNI.展开更多
基金Special Project for Protection and Utilization of Crop Germplasm Resources of the Ministry of Agriculture and Rural Affairs(No.2021-19210163,No.2021-19211041,No.202119210876)2021 Hubei Provincial Teaching Research Project:Research on course case base construction of agricultural engineering and information technology(No.2021351)。
文摘The chlorophyll content has a direct effect on photosynthesis of crops.In order to explore a quick and convenient method for estimating the chlorophyll content of Brassica napus and facilitate efficient crop monitoring,we measured the actual value of chlorophyll with a SPAD-502 chlorophyll detector,and collected aerial images of B.napus with an unmanned aerial vehicle(UAV)carrying a RGB camera in this study.The total number of 270samples collected images were divided into regions according to the planting conditions of different B.napus varieties in the field.Then,according to the empirical formula,there were 36 colors’characteristic parameters calculated and combined.To estimate the chlorophyll content of rape,189 samples were included in the modeling set,while the other 81 samples were enrolled in the validation set for testing the accuracy of this model.After the combination of R(red),G(green)and B(blue)color channels,the results showed that the color characteristics B/(R+G),b,B/G,(G-B)/(G+B),g-b were highly connected with the measured value of chlorophyll SPAD,and the correlation coefficient between the combination based on B/(R+G)and SPAD value was 0.747.With R2=0.805,RMSE=3.343,and RE=6.84%,the regression model created using random forest had superior outcomes,according to the model comparison.This study offers a new method for quickly estimating the amount of chlorophyll in rapeseed and a workable reference for crop monitoring using the UAV platform.
基金supported in part by the National Natural Science Foundation of China(Grant No.81703005)the Natural Science Foundation of Hunan Province(Grant Nos.2017JJ3195,2018JJ3317)the Key Research and Development Projects of Hunan Province(Grant No.2018SK2120).
文摘Background:Nuclear receptor binding SET domain protein-3(NSD3)is a histone lysine methyltransferase and a crucial regulator of carcinogenesis in several cancers.We aimed to investigate the prognostic value and potential function of NSD3 in 33 types of human cancer.Methods:The data were obtained from The Cancer Genome Atlas.Kaplan-Meier analysis,CIBERSORT,gene set enrichment analysis,and gene set variation analysis were performed.The expression of NSD3 was measured using quantitative real-time polymerase chain reaction and western blot.Results:The expression of NSD3 was altered in pan-cancer samples.Patients with higher levels of NDS3 generally had shorter overall survival and disease-specific survival.Levels of NSD3 were positively correlated with DNA copy number variation(CNV)in pan-cancer.NSD3 expression was also associated with tumor mutation burden and microsatellite instability.The levels of immune-cell infiltration differed significantly between high and low NSD3 expression.NSD3 negatively correlated with levels of CD8+T cells.Functional enrichment analysis showed that while NSD3 expression was positively associated with several immune cell-related and histone methylation-related pathways,it was negatively correlated with cell metabolism-related,drug transport-related,and drug metabolismrelated pathways.NSD3 levels in the cell lines tested were significantly different.In U251 and NCI-H23 cells,silencing NSD3 inhibited cell proliferation and promoted apoptosis.Conclusions:NSD3 expression was changed in pan-cancer samples that was also verified in cell lines.NSD3 was associated with CNV and immune-cell infiltration.A poor prognosis was predicted in patients with high expression of NSD3.NSD3 might hence be a potential marker for predicting tumor prognosis.
基金This work was financially supported by Scientific Research Fund Project of Education Department of Liaoning Province(LJKZ0960)Science Foundation of Shenyang Pharmaceutical University(GGJJ2021105).
文摘People continue to recognize the important role of Chinese medicine in the treatment of diseases,and there is an urgent need to clarify the mechanism of Chinese medicine.Because of the multi-target and multi-component characteristics of traditional Chinese medicine,it is difficult to elaborate from a single perspective.As a high-quality animal model,Caenorhabditis elegans with the characteristics of small size and fast reproduction,has been widely used in traditional Chinese medicine activity experiments.In this article,Caenorhabditis elegans,as model organisms used to screen the active components of traditional Chinese medicine were reviewed.
基金supported by the National Natural Science Foundation(No.52378020)Open Foundation of the State Key Laboratory of Subtropical Building and Urban Science(No.2023KA02)+1 种基金Fundamental Research Funds for the Central Universities(YCJJ20230576)Program for HUST Academic Frontier Youth Team(No.2019QYTD10).
文摘Design parameters at different scales in the pre-design phase could significantly impact both building energy consumption and photovoltaic(PV)power generation potential.However,existing studies often overlook the synergistic effects of design parameters across multiple scales(block-building-facade scales)when evaluating these aspects.This paper aims to propose a workflow for the assessing building energy consumption and PV power generation potential of office blocks applicable in the pre-schematic design phase considering the synergistic influence of multi-scale design parameters,using building typology and parametric modelling approach.The study proposed a multi-scale design parameter classification system combined with parametric modelling.The study investigated 80 office blocks in Wuhan as the study case,which were classified into array type and enclosed type.Correlation analysis and multiple regression equations were used to quantify the single versus synergistic effects of different scale design parameters.Results suggest that focusing solely on a single scale during the pre-design stage is typically inadequate for understanding building energy potential.In contrast,multi-scale synergistic analysis boosts energy use intensity(EUI)by 7.56%and net energy use intensity(NEUI)by 33.96%.Under multi-scale synergistic conditions,the EUI of array type is more influenced by the building design parameters,while the NEUI is effected by the balance of multi-scales design parameters.While the EUI of enclosed types exhibit balanced effects across multi-scale design parameters,with NEUI results aligning closely with PV power generation potential.Multiple regression equations highlight building density and shape factor as key influencers for both array and enclosure layouts.This study offers designers a flexible and scalable workflow for evaluating building energy consumption and PV power generation potential in the pre-design phase.The findings can guide nearly-zero energy urban block planning to achieve a balance between energy supply and demand.
基金supported by the National Natural Science Foundation of China (No. 21172178)
文摘A new rhodamine B derivative T1 has been rationally synthesized and displayed selective Pd（Ⅱ）-amplified absorbance and fluorescence emission above 540 nm in methanol-water. Upon the addition of Pd（Ⅱ）, the spirolactam ring was unfolded and a 1：1 metal-ligand complex formed, which can be used for ＇＇naked-eyes＂ detection. In addition, fluorescence imaging experiments of Pd^（2＋） in HepG2 living cells showed its valuable application in biological systems.
基金partially supported by the Natural Science Foundation of Hunan Province(2020JJ4005,2020JJ5421,2021JJ30096,China)National Natural Science Funds of China(82003931)+3 种基金The China Postdoctoral Science Foundation(2021M690974,China)Agricultural Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences(CAAS-ASTIP-IBFC04,China)Opening foundation of Key Laboratory of Study and Discovery of Small Targeted Molecules of Hunan Province(2022CG01,China)Key Research and Development Projects in Ningxia Autonomous Region(2022BFH02013,China)。
文摘Although carbon monoxide(CO)-based treatments have demonstrated the high cancer efficacy by promoting mitochondrial damage and core-region penetrating ability,the efficiency was often compromised by protective autophagy(mitophagy).Herein,cannabidiol(CBD)is integrated into biomimetic carbon monoxide nanocomplexes(HMPOC@M)to address this issue by inducing excessive autophagy.The biomimetic membrane not only prevents premature drugs leakage,but also prolongs blood circulation for tumor enrichment.After entering the acidic tumor microenvironment,carbon monoxide(CO)donors are stimulated by hydrogen oxide(H_(2)O_(2))to disintegrate into CO and Mn^(2+).The comprehensive effect of CO/Mn^(2+)and CBD can induce ROS-mediated cell apoptosis.In addition,HMPOC@Mmediated excessive autophagy can promote cancer cell death by increasing autophagic flux via classⅢPI3K/BECN1 complex activation and blocking autolysosome degradation via LAMP1 downregulation.Furthermore,in vivo experiments showed that HMPOC@M+laser strongly inhibited tumor growth and attenuated liver and lung metastases by downregulating VEGF and MMP9 proteins.This strategy may highlight the pro-death role of excessive autophagy in TNBC treatment,providing a novel yet versatile avenue to enhance the efficacy of CO treatments.Importantly,this work also indicated the applicability of CBD for triple-negative breast cancer(TNBC)therapy through excessive autophagy.
文摘To the Editor： Several kinds of malignant tumors show perineural invasion （PNI）, which can cause locoregional recurrence and reduce the likelihood of survival after surgery. Our previous study has shown that PNI in cervical cancer is associated with other pathology risk features, and other studies have shown that it is a predictor of poor prognosis. These findings highlight the importance of clarifying the molecular mechanism of PNI.
