Artificial fish nests are set in Lukou section and Hengyang section of Xiangjiang River from March 20 to May 10, 2019. The structure of artificial fish nest is bamboo frame, with the size of 2.5 m × 5.0 m × ...Artificial fish nests are set in Lukou section and Hengyang section of Xiangjiang River from March 20 to May 10, 2019. The structure of artificial fish nest is bamboo frame, with the size of 2.5 m × 5.0 m × 4 and a unit frame area of 60 m<sup>2</sup>. A total of 58,201 eggs were collected by 24 monitoring times, of which 30,441 were from Lukou and 27,760 from Hengyang. 3831 fish eggs identify 8 fish species using morphological or molecular biological methods. The results showed that the number of eggs peaked from the 8<sup>th</sup> day to the 24<sup>th</sup> day after the nest entered the water, and then decreased rapidly. There was significant negative correlation between egg number in per m<sup>2</sup> fish nest and days of fish nest (P < 0.01). The economic benefits of artificial fish nests were RMB 11.81 million. The artificial fish nest can not only increase the population of fish, but also has significant economic benefits.展开更多
Iron isotopic composition of the upper continental crust(UCC) is critical for understanding Fe mobilization and migration through the Earth. Because rocks exposed at Earth's surface have heterogeneous δ^(56)Fe, f...Iron isotopic composition of the upper continental crust(UCC) is critical for understanding Fe mobilization and migration through the Earth. Because rocks exposed at Earth's surface have heterogeneous δ^(56)Fe, finegrained clastic sediments can be used to estimate the average composition of UCC. In this study, we report δ^(56)Fe of loess-paleosol sequences from Yimaguan, Chinese Loess Plateau(CLP), to constrain the average Fe isotopic composition of UCC. The loess-paleosol sequences in this area formed in glacial-interglacial cycles and are characterized by varying degrees of weathering. Our data show that the loess-paleosol layers have extremely homogeneous Fe isotopic compositions with δ^(56)Fe ranging from 0.06‰ to 0.12‰, regardless of variations in the major element composition and weathering intensity. Our study indicates that since Fe isotopes are not significantly fractionated during loess deposition, the loess can be regarded as representative of UCC. It follows that the average δ^(56)Fe of UCC is 0.09‰± 0.03‰(2SD), consistent with previous estimates based on igneous rock data.展开更多
Zircon stability in silicate melts-which can be quantitatively constrained by laboratory measurements of zircon saturation-is important for understanding the evolution of magma.Although the original zircon saturation ...Zircon stability in silicate melts-which can be quantitatively constrained by laboratory measurements of zircon saturation-is important for understanding the evolution of magma.Although the original zircon saturation model proposed by Watson and Harrison(Earth Planet Sci Lett 64(2):295-304,1983) is widely cited and has been updated recently,the three main models currently in use may generate large uncertainties due to extrapolation beyond their respective calibrated ranges.This paper reviews and updates zircon saturation models developed with temperature and compositional parameters.All available data on zircon saturation ranging in composition from mafic to silicic(and/or peralkaline to peraluminous)at temperatures from 750 to 1400℃ were collected to develop two refined models(1 and 2) that may be applied to the wider range of compositions.Model 1 is given by lnCZr(melt)=(14.297±0.308)+(0.964 ± 0.066).M-(11113±374)/r,and model 2 given by lnCZr(melt)=(18.99±0.423)-(1.069±0.102)·lnG-(12288±593)/T,where CZr(melt) is the Zr concentration of the melt in ppm and parameters M [=(Na+K+2 Ca)/(Al·Si)](cation ratios) and G [=(3·Al2 O3+SiO2)/(Na2-O+K2 O+CaO+MgO+FeO)](molar proportions)represent the melt composition.The errors are at one sigma,and T is the temperature in Kelvin.Before applying these models to natural rocks,it is necessary to ensure that the zircon used to date is crystallized from the host magmatic rock.Assessment of the application of both new and old models to natural rocks suggests that model 1 may be the best for magmatic temperature estimates of metaluminous to peraluminous rocks and that model 2 may be the best for estimating magmatic temperatures of alkaline to peralkaline rocks.展开更多
Even with an unprecedented breakthrough of deep learning in electroencephalography(EEG),collecting adequate labelled samples is a critical problem due to laborious and time‐consuming labelling.Recent study proposed t...Even with an unprecedented breakthrough of deep learning in electroencephalography(EEG),collecting adequate labelled samples is a critical problem due to laborious and time‐consuming labelling.Recent study proposed to solve the limited label problem via domain adaptation methods.However,they mainly focus on reducing domain discrepancy without considering task‐specific decision boundaries,which may lead to feature distribution overmatching and therefore make it hard to match within a large domain gap completely.A novel self‐training maximum classifier discrepancy method for EEG classification is proposed in this study.The proposed approach detects samples from a new subject beyond the support of the existing source subjects by maximising the discrepancies between two classifiers'outputs.Besides,a self‐training method that uses unlabelled test data to fully use knowledge from the new subject and further reduce the domain gap is proposed.Finally,a 3D Cube that incorporates the spatial and frequency information of the EEG data to create input features of a Convolutional Neural Network(CNN)is constructed.Extensive experiments on SEED and SEED‐IV are conducted.The experimental evaluations exhibit that the proposed method can effectively deal with domain transfer problems and achieve better performance.展开更多
Objective:To investigate the therapeutic effects of co-transplantation with Schwann cells(SCs) and human embryonic nerve stem cells(NSCs) on macaque Parkinson’s disease(PD).Methods: Macaque autologous SCs and h...Objective:To investigate the therapeutic effects of co-transplantation with Schwann cells(SCs) and human embryonic nerve stem cells(NSCs) on macaque Parkinson’s disease(PD).Methods: Macaque autologous SCs and human embryonic NSCs were adopled for the treatment of macaque PD.Results:Six months after transplantation,positron emission computerized tomography showed that <sup>18</sup>F-FP-β-CIT was significantly concentrated in the injured striatum in the cotransplanted group.Immunohistoehemieal staining of transplanted area tissue showed migration of tyrosine hydroxylase positive cells from the transplant area to the surrounding area was significantly increased in the co-transplanted group.Conclusions:Co-transplantation of SCs and NSCs could effectively cure PD in macaques.SCs harvested from the autologous peripheral nerves can avoid rejection and the ethics problems,so it is expected to be applied clinically.展开更多
With the rapid development of wireless local area network (WLAN) technology, an important target of indoor positioning systems is to improve the positioning accuracy while reducing the online calibration effort to o...With the rapid development of wireless local area network (WLAN) technology, an important target of indoor positioning systems is to improve the positioning accuracy while reducing the online calibration effort to overcome signal time-varying. A novel fingerprint positioning algorithm, known as the adaptive radio map with updated method based on hidden Markov model (HMM), is proposed. It is shown that by using a collection of user traces that can be cheaply obtained, the proposed algorithm can take advantage of these data to update the labeled calibration data to further improve the position estimation accuracy. This algorithm is a combination of machine learning, information gain theory and fingerprinting. By collecting data and testing the algorithm in a realistic indoor WLAN environment, the experiment results indicate that, compared with the widely used K nearest neighbor algorithm, the proposed algorithm can improve the positioning accuracy while greatly reduce the calibration effort.展开更多
Background:Posttraumatic stress disorder(PTSD)has been associated with volumetric and white matter microstructural changes among general and veteran populations.However,regions implicated have greatly varied and often...Background:Posttraumatic stress disorder(PTSD)has been associated with volumetric and white matter microstructural changes among general and veteran populations.However,regions implicated have greatly varied and often conflict between studies,potentially due to confounding comorbidities within samples.This study compared grey matter volume and white matter microstructure among Australian combat veterans with and without a lifetime diagnosis of PTSD,in a homogenous sample assessed for known confounding comorbidities.Methods:Sixty-eight male trauma-exposed veterans(16 PTSD-diagnosed;mean age 69 years)completed a battery of psychometric assessments and underwent magnetic resonance and diffusion tensor imaging.Analyses included tractbased spatial statistics,voxel-wise analyses,diffusion connectome-based group-wise analysis,and volumetric analysis.Results:Significantly smaller grey matter volumes were observed in the left prefrontal cortex(P=0.026),bilateral middle frontal gyrus(P=0.021),and left anterior insula(P=0.048)in the PTSD group compared to controls.Significant negative correlations were found between PTSD symptom severity and fractional anisotropy values in the left corticospinal tract(R^(2)=0.34,P=0.024)and left inferior cerebellar peduncle(R^(2)=0.62,P=0.016).No connectome-based differences in white matter properties were observed.Conclusions:Findings from this study reinforce reports of white matter alterations,as indicated by reduced fractional anisotropy values,in relation to PTSD symptom severity,as well as patterns of reduced volume in the prefrontal cortex.These results contribute to the developing profile of neuroanatomical differences uniquely attributable to veterans who suffer from chronic PTSD.展开更多
BACKGROUND: Due to the lack of autograft transplant rejection, Schwann cells (SCs) can promote the proliferation of embryonic stem cells and the induction of dopaminergic neurons. Mesencephalic stem cells can be in...BACKGROUND: Due to the lack of autograft transplant rejection, Schwann cells (SCs) can promote the proliferation of embryonic stem cells and the induction of dopaminergic neurons. Mesencephalic stem cells can be induced to produce dopaminergic neurons. The therapeutic effects of co-grafts of SCs and neural stem cells (NSCs) deserves further study and verification in Parkinsonian animal models. OBJECTIVE: To investigate the effects of Schwann cells and mesencephalic NSC co-grafts in Parkinsonian animal models on animal behavior and histology. DESIGN: Randomized controlled experiment. SETTING: Fudan University; Institute of Neuroscience, Chinese Academy of Sciences. MATERIALS: The following animals were obtained from the Experimental Animal Center, Shanghai Institute for Biological Science, Chinese Academy of Sciences: 5 Sprague-Dawley rats, embryonic day (E) 13-16; 16 neonatal Sprague-Dawley rats, postnatal day 1-3; and 18 adult SD rats of both genders. Animal experimentation met animal ethical approval. METHODS: The experiment was performed at the Department of Anatomy, Histology and Embryology, Shanghai Medical Center, Fudan University from September 2005 to January 2007. The mesencephalic NSCs were obtained from the brains of SD rats at E 13-16, and SCs were harvested from the sciatic nerves of neonatal rats at day 1-3. Hemiparkinsonian rats (n =18) were selected for transplantation after estimating rotational behavior in response to apomorphine and were randomly assigned to three groups: control group, NSC group, and co-graft group. There were 6 rats in each group. Either phosphate buffered saline (PBS), NSCs, or SCs plus NSCs were transplanted into the right neostriatum of Parkinsonian rats, respectively. MAIN OUTCOME MEASURES: (1) Rotational behavior was induced by apomorphine (0.05 mg/kg, i.p.) 2, 4, 6, 8, and 10 weeks after transplantation, and the number of rotations were counted. (2) Differentiation and survival of dopaminergic neurons in the right neostriatum were quantified by tyrosine hydroxylase immunohistochemistry 10 weeks after grafting. RESULTS: All 18 Parkinsonian rats were included in the final analysis,without any loss. (1)Rotation behavior and turning: Compared with the control group, the percent of apomorphine-induced rotations were significantly decreased (P 〈 0.01 ) in both the NSC group and co-graft group. (2)Differentiation and survival of dopaminergic neurons in each group: TH-immunoreactive neurons were detected in the striatum of both the NSC group and co-graft group 10 weeks after transplantation. The neuronal volume, size of the nucleus, and neuronal numbers were larger in the co-graft group compared to the NSC group (P 〈 0.05). CONCLUSION: SC and NSC co-grafts not only improve Parkinsonian behavior in rats, but also improve the survival of NSCs.展开更多
With the rapid development of WLAN( Wireless Local Area Network) technology,an important target of indoor positioning systems is to improve the positioning accuracy while reducing the online computation.In this paper,...With the rapid development of WLAN( Wireless Local Area Network) technology,an important target of indoor positioning systems is to improve the positioning accuracy while reducing the online computation.In this paper,it proposes a novel fingerprint positioning algorithm known as semi-supervised affinity propagation clustering based on distance function constraints. We show that by employing affinity propagation techniques,it is able to use a fractional labeled data to adjust similarity matrix of signal space to cluster reference points with high accuracy. The semi-supervised APC uses a combination of machine learning,clustering analysis and fingerprinting algorithm. By collecting data and testing our algorithm in a realistic indoor WLAN environment,the experimental results indicate that the proposed algorithm can improve positioning accuracy while reduce the online localization computation,as compared with the widely used K nearest neighbor and maximum likelihood estimation algorithms.展开更多
This report describes an outbreak and treatment of pneumonia and enteritis in a snake farm with more than 3000 snakes containing Elaphe carinata(one-year-old)and Ptyas mucosus(three-month-old)seedlings in Huanggang,Hu...This report describes an outbreak and treatment of pneumonia and enteritis in a snake farm with more than 3000 snakes containing Elaphe carinata(one-year-old)and Ptyas mucosus(three-month-old)seedlings in Huanggang,Hubei,China.Gentamicin was used once in the early stage as treatment,administered orally with water or feed by owners,but mortality increased.Lobar pneumonia was confirmed by dissection and histopathology in infected snakes.Four main pathogenic bacteria were isolated and identified with culture and 16S rRNA sequencing:Staphylococcus sciuri,Salmonella enteritis,Vagococcus fluvialis and Providencia vermicola.Drug susceptibility tests were performed,and amikacin,gentamicin and cefitriaxone were chosen accordingly.After two rounds of treatment,the clinical signs for Elaphe carinata were under control,and the mortality was close to 0%after treatment.However,treatments for Ptyas mucosus seedlings did not work well,potentially because of poor administration technique and weak body condition.展开更多
Objective:This study aimed to evaluate the acute and chronic cytotoxicity and genotoxicity of 2,6-dichloro-1,4-benzoquinone(2,6-DCBQ).Methods:NIH3T3 cells were exposed to 2,6-DCBQ for 3 and 72 h,and relative cell viab...Objective:This study aimed to evaluate the acute and chronic cytotoxicity and genotoxicity of 2,6-dichloro-1,4-benzoquinone(2,6-DCBQ).Methods:NIH3T3 cells were exposed to 2,6-DCBQ for 3 and 72 h,and relative cell viability was calculated.NIH3T3 cells were treated with different concentrations of 2,6-DCBQ for 24 h.The solvent and positive controls were dimethyl sulfoxide(DMSO)and 0.5μmol/L ethylmethylsulfone,respectively.The values of Olive tail moment were measured by comet assay.NIH3T3 cells were then simultaneously treated with 5μg/mL cytochalasin B and different concentrations of 2,6-DCBQ.The solvent and positive controls were DMSO and 1μmol/L mitomycin C,respectively.Micronucleus rates were calculated after 48 h.Results:The half lethal doses of 2,6-DCBQ in NIH3T3 cells were 64.93μmol/L for 3 hand 13.46μmol/L for 72 h.The values of Olive tail moment in the 7.5 and 10μmol/L groups were significantly higher than those in the solvent control(P<0.05).Moreover,the micronucleus rates in the 10 and 15μmol/L groups were significantly higher than those in the solvent control(P <0.05).The results of comet assay and micronucleus test showed a dose-response relationship.Conclusion:2,6-DCBQ exhibited strong cytotoxicity and induced DNA and chromosomal damage in NIH3T3 cells.展开更多
Hexagrammos otakii is an important kind of economic fish species in East Asia. However, wild H. otakii resources have declined sharply in recent years as a result of human disturbance and habitat destruction. Thus, it...Hexagrammos otakii is an important kind of economic fish species in East Asia. However, wild H. otakii resources have declined sharply in recent years as a result of human disturbance and habitat destruction. Thus, it is crucial to protect the current resources of H. otakii. In this study, 35 novel single nucleotide polymorphism (SNP) markers were developed based on restriction-site associated DNA sequencing. The results showed that the observed heterozygosity and expected heterozygosity ranged from 0.1875 to 0.6562 and 0.2679 to 0.5079, respectively. The minor allele frequency ranged from 0.1875 to 0.4375. Polymorphic information content ranged from 0.229 to 0.375. Six SNPs were found to be deviated significantly from the HWE (P H. otakii.展开更多
Starch is a major storage carbohydrate in plants and is critical in crop yield and quality.Starch synthesis is intricately regulated by internal metabolic processes and external environmental cues;however,the precise ...Starch is a major storage carbohydrate in plants and is critical in crop yield and quality.Starch synthesis is intricately regulated by internal metabolic processes and external environmental cues;however,the precise molecular mechanisms governing this process remain largely unknown.In this study,we revealed that high red to far-red(high R:FR)light significantly induces the synthesis of leaf starch and the expression of synthesis-related genes,whereas low R:FR light suppress these processes.Arabidopsis phytochrome B(phyB),the primary R and FR photoreceptor,was identified as a critical positive regulator in this process.Downstream of phyB,basic leucine zipper transcription factor ELONGATED HYPOCOTYL5(HY5)was found to enhance starch synthesis,whereas the basic helix-loop-helix transcription factors PHYTOCHROME INTERACTING FACTORs(PIF3,PIF4,and PIF5)inhibit starch synthesis in Arabidopsis leaves.Notably,HY5 and PIFs directly compete for binding to a shared G-box cis-element in the promoter region of genes encoding starch synthases GBSS,SS3,and SS4,which leads to antagonistic regulation of their expression and,consequently,starch synthesis.Our findings highlight the vital role of phyB in enhancing starch synthesis by stabilizing HY5 and facilitating PIFs degradation under high R:FR light conditions.Conversely,under low R:FR light,PIFs predominantly inhibit starch synthesis.This study provides insight into the physiological and molecular functions of phyB and its downstream transcription factors HY5 and PIFs in starch synthesis regulation,shedding light on the regulatory mechanism by which plants synchronize dynamic light signals with metabolic cues to module starch synthesis.展开更多
文摘Artificial fish nests are set in Lukou section and Hengyang section of Xiangjiang River from March 20 to May 10, 2019. The structure of artificial fish nest is bamboo frame, with the size of 2.5 m × 5.0 m × 4 and a unit frame area of 60 m<sup>2</sup>. A total of 58,201 eggs were collected by 24 monitoring times, of which 30,441 were from Lukou and 27,760 from Hengyang. 3831 fish eggs identify 8 fish species using morphological or molecular biological methods. The results showed that the number of eggs peaked from the 8<sup>th</sup> day to the 24<sup>th</sup> day after the nest entered the water, and then decreased rapidly. There was significant negative correlation between egg number in per m<sup>2</sup> fish nest and days of fish nest (P < 0.01). The economic benefits of artificial fish nests were RMB 11.81 million. The artificial fish nest can not only increase the population of fish, but also has significant economic benefits.
基金financially supported by the National Science Foundation of China(41173031,41325011 and 41503001)the Fundamental Research Funds for the Central Universities(WK3410000004)
文摘Iron isotopic composition of the upper continental crust(UCC) is critical for understanding Fe mobilization and migration through the Earth. Because rocks exposed at Earth's surface have heterogeneous δ^(56)Fe, finegrained clastic sediments can be used to estimate the average composition of UCC. In this study, we report δ^(56)Fe of loess-paleosol sequences from Yimaguan, Chinese Loess Plateau(CLP), to constrain the average Fe isotopic composition of UCC. The loess-paleosol sequences in this area formed in glacial-interglacial cycles and are characterized by varying degrees of weathering. Our data show that the loess-paleosol layers have extremely homogeneous Fe isotopic compositions with δ^(56)Fe ranging from 0.06‰ to 0.12‰, regardless of variations in the major element composition and weathering intensity. Our study indicates that since Fe isotopes are not significantly fractionated during loess deposition, the loess can be regarded as representative of UCC. It follows that the average δ^(56)Fe of UCC is 0.09‰± 0.03‰(2SD), consistent with previous estimates based on igneous rock data.
基金financially supported by the Strategic Priority Research Program (B) of the Chinese Academy of Sciences (Grant No. XDB18010402)the National Natural Science Foundation of China (Grant No. 41702224)the Pearl River Talent Plan of Guangdong Province
文摘Zircon stability in silicate melts-which can be quantitatively constrained by laboratory measurements of zircon saturation-is important for understanding the evolution of magma.Although the original zircon saturation model proposed by Watson and Harrison(Earth Planet Sci Lett 64(2):295-304,1983) is widely cited and has been updated recently,the three main models currently in use may generate large uncertainties due to extrapolation beyond their respective calibrated ranges.This paper reviews and updates zircon saturation models developed with temperature and compositional parameters.All available data on zircon saturation ranging in composition from mafic to silicic(and/or peralkaline to peraluminous)at temperatures from 750 to 1400℃ were collected to develop two refined models(1 and 2) that may be applied to the wider range of compositions.Model 1 is given by lnCZr(melt)=(14.297±0.308)+(0.964 ± 0.066).M-(11113±374)/r,and model 2 given by lnCZr(melt)=(18.99±0.423)-(1.069±0.102)·lnG-(12288±593)/T,where CZr(melt) is the Zr concentration of the melt in ppm and parameters M [=(Na+K+2 Ca)/(Al·Si)](cation ratios) and G [=(3·Al2 O3+SiO2)/(Na2-O+K2 O+CaO+MgO+FeO)](molar proportions)represent the melt composition.The errors are at one sigma,and T is the temperature in Kelvin.Before applying these models to natural rocks,it is necessary to ensure that the zircon used to date is crystallized from the host magmatic rock.Assessment of the application of both new and old models to natural rocks suggests that model 1 may be the best for magmatic temperature estimates of metaluminous to peraluminous rocks and that model 2 may be the best for estimating magmatic temperatures of alkaline to peralkaline rocks.
基金supported in part by the National Natural Science Foundation of China under Grants 61866039in part by the Natural Science Foundation of Chongqing,China(No.cstc2019jscxmbdxX0021)+1 种基金in part by the Excellent Youths Project for Basic Research of Yunnan Province(No.202101AW070015)in part by the Key Cooperation Project of Chongqing Municipal Education Commission(No.HZ2021008).
文摘Even with an unprecedented breakthrough of deep learning in electroencephalography(EEG),collecting adequate labelled samples is a critical problem due to laborious and time‐consuming labelling.Recent study proposed to solve the limited label problem via domain adaptation methods.However,they mainly focus on reducing domain discrepancy without considering task‐specific decision boundaries,which may lead to feature distribution overmatching and therefore make it hard to match within a large domain gap completely.A novel self‐training maximum classifier discrepancy method for EEG classification is proposed in this study.The proposed approach detects samples from a new subject beyond the support of the existing source subjects by maximising the discrepancies between two classifiers'outputs.Besides,a self‐training method that uses unlabelled test data to fully use knowledge from the new subject and further reduce the domain gap is proposed.Finally,a 3D Cube that incorporates the spatial and frequency information of the EEG data to create input features of a Convolutional Neural Network(CNN)is constructed.Extensive experiments on SEED and SEED‐IV are conducted.The experimental evaluations exhibit that the proposed method can effectively deal with domain transfer problems and achieve better performance.
基金supported by the Hainan Natural Science Foundation, NO.310147
文摘Objective:To investigate the therapeutic effects of co-transplantation with Schwann cells(SCs) and human embryonic nerve stem cells(NSCs) on macaque Parkinson’s disease(PD).Methods: Macaque autologous SCs and human embryonic NSCs were adopled for the treatment of macaque PD.Results:Six months after transplantation,positron emission computerized tomography showed that <sup>18</sup>F-FP-β-CIT was significantly concentrated in the injured striatum in the cotransplanted group.Immunohistoehemieal staining of transplanted area tissue showed migration of tyrosine hydroxylase positive cells from the transplant area to the surrounding area was significantly increased in the co-transplanted group.Conclusions:Co-transplantation of SCs and NSCs could effectively cure PD in macaques.SCs harvested from the autologous peripheral nerves can avoid rejection and the ethics problems,so it is expected to be applied clinically.
基金supported by the National Natural Science Foundation of China(61571162)the Major National Science and Technology Project(2014ZX03004003-005)
文摘With the rapid development of wireless local area network (WLAN) technology, an important target of indoor positioning systems is to improve the positioning accuracy while reducing the online calibration effort to overcome signal time-varying. A novel fingerprint positioning algorithm, known as the adaptive radio map with updated method based on hidden Markov model (HMM), is proposed. It is shown that by using a collection of user traces that can be cheaply obtained, the proposed algorithm can take advantage of these data to update the labeled calibration data to further improve the position estimation accuracy. This algorithm is a combination of machine learning, information gain theory and fingerprinting. By collecting data and testing the algorithm in a realistic indoor WLAN environment, the experiment results indicate that, compared with the widely used K nearest neighbor algorithm, the proposed algorithm can improve the positioning accuracy while greatly reduce the calibration effort.
基金RSL Queensland funded this study as part of the PTSD Initiative at the Gallipoli Medical Research Foundation.The Australian Government Department of Veterans’Affairs provided transport for eligible participants。
文摘Background:Posttraumatic stress disorder(PTSD)has been associated with volumetric and white matter microstructural changes among general and veteran populations.However,regions implicated have greatly varied and often conflict between studies,potentially due to confounding comorbidities within samples.This study compared grey matter volume and white matter microstructure among Australian combat veterans with and without a lifetime diagnosis of PTSD,in a homogenous sample assessed for known confounding comorbidities.Methods:Sixty-eight male trauma-exposed veterans(16 PTSD-diagnosed;mean age 69 years)completed a battery of psychometric assessments and underwent magnetic resonance and diffusion tensor imaging.Analyses included tractbased spatial statistics,voxel-wise analyses,diffusion connectome-based group-wise analysis,and volumetric analysis.Results:Significantly smaller grey matter volumes were observed in the left prefrontal cortex(P=0.026),bilateral middle frontal gyrus(P=0.021),and left anterior insula(P=0.048)in the PTSD group compared to controls.Significant negative correlations were found between PTSD symptom severity and fractional anisotropy values in the left corticospinal tract(R^(2)=0.34,P=0.024)and left inferior cerebellar peduncle(R^(2)=0.62,P=0.016).No connectome-based differences in white matter properties were observed.Conclusions:Findings from this study reinforce reports of white matter alterations,as indicated by reduced fractional anisotropy values,in relation to PTSD symptom severity,as well as patterns of reduced volume in the prefrontal cortex.These results contribute to the developing profile of neuroanatomical differences uniquely attributable to veterans who suffer from chronic PTSD.
文摘BACKGROUND: Due to the lack of autograft transplant rejection, Schwann cells (SCs) can promote the proliferation of embryonic stem cells and the induction of dopaminergic neurons. Mesencephalic stem cells can be induced to produce dopaminergic neurons. The therapeutic effects of co-grafts of SCs and neural stem cells (NSCs) deserves further study and verification in Parkinsonian animal models. OBJECTIVE: To investigate the effects of Schwann cells and mesencephalic NSC co-grafts in Parkinsonian animal models on animal behavior and histology. DESIGN: Randomized controlled experiment. SETTING: Fudan University; Institute of Neuroscience, Chinese Academy of Sciences. MATERIALS: The following animals were obtained from the Experimental Animal Center, Shanghai Institute for Biological Science, Chinese Academy of Sciences: 5 Sprague-Dawley rats, embryonic day (E) 13-16; 16 neonatal Sprague-Dawley rats, postnatal day 1-3; and 18 adult SD rats of both genders. Animal experimentation met animal ethical approval. METHODS: The experiment was performed at the Department of Anatomy, Histology and Embryology, Shanghai Medical Center, Fudan University from September 2005 to January 2007. The mesencephalic NSCs were obtained from the brains of SD rats at E 13-16, and SCs were harvested from the sciatic nerves of neonatal rats at day 1-3. Hemiparkinsonian rats (n =18) were selected for transplantation after estimating rotational behavior in response to apomorphine and were randomly assigned to three groups: control group, NSC group, and co-graft group. There were 6 rats in each group. Either phosphate buffered saline (PBS), NSCs, or SCs plus NSCs were transplanted into the right neostriatum of Parkinsonian rats, respectively. MAIN OUTCOME MEASURES: (1) Rotational behavior was induced by apomorphine (0.05 mg/kg, i.p.) 2, 4, 6, 8, and 10 weeks after transplantation, and the number of rotations were counted. (2) Differentiation and survival of dopaminergic neurons in the right neostriatum were quantified by tyrosine hydroxylase immunohistochemistry 10 weeks after grafting. RESULTS: All 18 Parkinsonian rats were included in the final analysis,without any loss. (1)Rotation behavior and turning: Compared with the control group, the percent of apomorphine-induced rotations were significantly decreased (P 〈 0.01 ) in both the NSC group and co-graft group. (2)Differentiation and survival of dopaminergic neurons in each group: TH-immunoreactive neurons were detected in the striatum of both the NSC group and co-graft group 10 weeks after transplantation. The neuronal volume, size of the nucleus, and neuronal numbers were larger in the co-graft group compared to the NSC group (P 〈 0.05). CONCLUSION: SC and NSC co-grafts not only improve Parkinsonian behavior in rats, but also improve the survival of NSCs.
基金Sponsored by the National Natural Science Foundation of China(Grant No.61101122 and 61071105)
文摘With the rapid development of WLAN( Wireless Local Area Network) technology,an important target of indoor positioning systems is to improve the positioning accuracy while reducing the online computation.In this paper,it proposes a novel fingerprint positioning algorithm known as semi-supervised affinity propagation clustering based on distance function constraints. We show that by employing affinity propagation techniques,it is able to use a fractional labeled data to adjust similarity matrix of signal space to cluster reference points with high accuracy. The semi-supervised APC uses a combination of machine learning,clustering analysis and fingerprinting algorithm. By collecting data and testing our algorithm in a realistic indoor WLAN environment,the experimental results indicate that the proposed algorithm can improve positioning accuracy while reduce the online localization computation,as compared with the widely used K nearest neighbor and maximum likelihood estimation algorithms.
基金supported by the Fundamental Research Funds for the Central Universities,China(2662017QD015).
文摘This report describes an outbreak and treatment of pneumonia and enteritis in a snake farm with more than 3000 snakes containing Elaphe carinata(one-year-old)and Ptyas mucosus(three-month-old)seedlings in Huanggang,Hubei,China.Gentamicin was used once in the early stage as treatment,administered orally with water or feed by owners,but mortality increased.Lobar pneumonia was confirmed by dissection and histopathology in infected snakes.Four main pathogenic bacteria were isolated and identified with culture and 16S rRNA sequencing:Staphylococcus sciuri,Salmonella enteritis,Vagococcus fluvialis and Providencia vermicola.Drug susceptibility tests were performed,and amikacin,gentamicin and cefitriaxone were chosen accordingly.After two rounds of treatment,the clinical signs for Elaphe carinata were under control,and the mortality was close to 0%after treatment.However,treatments for Ptyas mucosus seedlings did not work well,potentially because of poor administration technique and weak body condition.
基金supported by grants from National Natural Science Foundation of China(No.81560524,81360421)the China Postdoctoral Science Foundation (No.2013M540686, 2014T70839)+1 种基金the Guangxi Natural Science Foundation(No.2012GXNSFBA053109)the Outstanding Young Middle-aged Excellent Teachers’ Training in Higher Education Institutions of Guangxi
文摘Objective:This study aimed to evaluate the acute and chronic cytotoxicity and genotoxicity of 2,6-dichloro-1,4-benzoquinone(2,6-DCBQ).Methods:NIH3T3 cells were exposed to 2,6-DCBQ for 3 and 72 h,and relative cell viability was calculated.NIH3T3 cells were treated with different concentrations of 2,6-DCBQ for 24 h.The solvent and positive controls were dimethyl sulfoxide(DMSO)and 0.5μmol/L ethylmethylsulfone,respectively.The values of Olive tail moment were measured by comet assay.NIH3T3 cells were then simultaneously treated with 5μg/mL cytochalasin B and different concentrations of 2,6-DCBQ.The solvent and positive controls were DMSO and 1μmol/L mitomycin C,respectively.Micronucleus rates were calculated after 48 h.Results:The half lethal doses of 2,6-DCBQ in NIH3T3 cells were 64.93μmol/L for 3 hand 13.46μmol/L for 72 h.The values of Olive tail moment in the 7.5 and 10μmol/L groups were significantly higher than those in the solvent control(P<0.05).Moreover,the micronucleus rates in the 10 and 15μmol/L groups were significantly higher than those in the solvent control(P <0.05).The results of comet assay and micronucleus test showed a dose-response relationship.Conclusion:2,6-DCBQ exhibited strong cytotoxicity and induced DNA and chromosomal damage in NIH3T3 cells.
文摘Hexagrammos otakii is an important kind of economic fish species in East Asia. However, wild H. otakii resources have declined sharply in recent years as a result of human disturbance and habitat destruction. Thus, it is crucial to protect the current resources of H. otakii. In this study, 35 novel single nucleotide polymorphism (SNP) markers were developed based on restriction-site associated DNA sequencing. The results showed that the observed heterozygosity and expected heterozygosity ranged from 0.1875 to 0.6562 and 0.2679 to 0.5079, respectively. The minor allele frequency ranged from 0.1875 to 0.4375. Polymorphic information content ranged from 0.229 to 0.375. Six SNPs were found to be deviated significantly from the HWE (P H. otakii.
基金supported by the National Natural Science Foundation of China(32270263)the Shandong Provincial Natural Science Foundation(ZR2022QC095,ZR2020MC023,ZR2022MC019).
文摘Starch is a major storage carbohydrate in plants and is critical in crop yield and quality.Starch synthesis is intricately regulated by internal metabolic processes and external environmental cues;however,the precise molecular mechanisms governing this process remain largely unknown.In this study,we revealed that high red to far-red(high R:FR)light significantly induces the synthesis of leaf starch and the expression of synthesis-related genes,whereas low R:FR light suppress these processes.Arabidopsis phytochrome B(phyB),the primary R and FR photoreceptor,was identified as a critical positive regulator in this process.Downstream of phyB,basic leucine zipper transcription factor ELONGATED HYPOCOTYL5(HY5)was found to enhance starch synthesis,whereas the basic helix-loop-helix transcription factors PHYTOCHROME INTERACTING FACTORs(PIF3,PIF4,and PIF5)inhibit starch synthesis in Arabidopsis leaves.Notably,HY5 and PIFs directly compete for binding to a shared G-box cis-element in the promoter region of genes encoding starch synthases GBSS,SS3,and SS4,which leads to antagonistic regulation of their expression and,consequently,starch synthesis.Our findings highlight the vital role of phyB in enhancing starch synthesis by stabilizing HY5 and facilitating PIFs degradation under high R:FR light conditions.Conversely,under low R:FR light,PIFs predominantly inhibit starch synthesis.This study provides insight into the physiological and molecular functions of phyB and its downstream transcription factors HY5 and PIFs in starch synthesis regulation,shedding light on the regulatory mechanism by which plants synchronize dynamic light signals with metabolic cues to module starch synthesis.