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An Evaluation of Artificial Fish Nest for Assessment Enhancement Effects of Fishery Resources of in Xiangjiang River 被引量:1
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作者 ying xia Qiang Hu Xiping Yuan 《Journal of Geoscience and Environment Protection》 2020年第6期15-19,共5页
Artificial fish nests are set in Lukou section and Hengyang section of Xiangjiang River from March 20 to May 10, 2019. The structure of artificial fish nest is bamboo frame, with the size of 2.5 m × 5.0 m × ... Artificial fish nests are set in Lukou section and Hengyang section of Xiangjiang River from March 20 to May 10, 2019. The structure of artificial fish nest is bamboo frame, with the size of 2.5 m × 5.0 m × 4 and a unit frame area of 60 m<sup>2</sup>. A total of 58,201 eggs were collected by 24 monitoring times, of which 30,441 were from Lukou and 27,760 from Hengyang. 3831 fish eggs identify 8 fish species using morphological or molecular biological methods. The results showed that the number of eggs peaked from the 8<sup>th</sup> day to the 24<sup>th</sup> day after the nest entered the water, and then decreased rapidly. There was significant negative correlation between egg number in per m<sup>2</sup> fish nest and days of fish nest (P < 0.01). The economic benefits of artificial fish nests were RMB 11.81 million. The artificial fish nest can not only increase the population of fish, but also has significant economic benefits. 展开更多
关键词 Artificial Fish Nest Enhancement Effects of Fishery Resources Xiangjiang River
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茶白星病不同病情等级下叶际细菌群落多样性与功能预测 被引量:7
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作者 周凌云 向芬 +5 位作者 刘红艳 李维 周琳 银霞 曾泽萱 王振中 《茶叶通讯》 北大核心 2019年第1期24-31,共8页
茶白星病是一种多年来严重为害高海拔茶园的叶部病害,为了突破该病害生物防控的技术瓶颈,明确不同茶白星病病情等级下叶际细菌群落具有重要意义。运用高通量测序方法比较分析3个不同发病等级的茶白星病叶片(BCK、B2和B4)中细菌群落组成... 茶白星病是一种多年来严重为害高海拔茶园的叶部病害,为了突破该病害生物防控的技术瓶颈,明确不同茶白星病病情等级下叶际细菌群落具有重要意义。运用高通量测序方法比较分析3个不同发病等级的茶白星病叶片(BCK、B2和B4)中细菌群落组成,结果发现:在门水平上,3个病情等级下优势门均为Acidobacteria (酸杆菌门)、Bacteroidetes(拟杆菌门)、Proteobacteria(变形菌门)和Firmicutes(厚壁菌门),其中Proteobacteria(变形菌门)占总数的73.53%~90.25%。相对丰度多样性与茶白星病的发病病情等级呈正相关的为Acidobacteria(酸杆菌门)和Bacteroidetes(拟杆菌门)。在属水平上,随着病情等级升高,叶际细菌群落整体OTUs、差异OTUs及其多样性均上升。优势属Methylobacterium(甲醇杆菌属)与Hymenobacter(膜杆菌属)的相对丰度与病情等级呈正相关,而Ralstonia(雷氏菌属)与Stenotrophomonas(寡养单胞菌)则相反,表明茶白星病菌与叶际细菌存在协同作用,细菌群落在3个不同病情等级下存在差异,为进一步研究茶叶叶际微生态防控茶白星病提供了科学依据。 展开更多
关键词 茶叶 叶际细菌 茶白星病 细菌群落 多样性 功能预测
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百年探秘求真相——茶白星病 被引量:7
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作者 周凌云 刘红艳 +3 位作者 李维 向芬 银霞 曾泽萱 《中国茶叶》 2020年第2期11-13,23,共4页
茶白星病是我国茶区常发真菌病害,近年发生面积及为害程度呈上升趋势,不利于我国茶产业的可持续发展。本文重点介绍了茶白星病病原菌的鉴定,并进一步综述其分布与为害、发生规律和防控措施,以供茶叶技术人员和生产者参考。
关键词 茶白星病 发生特点 防控措施
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贵州省2013-2021年羊种布鲁氏菌分离株的多位点可变数目串联重复序列分析 被引量:1
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作者 王月 谭勤琴 +5 位作者 刘英 杨幸贵 营夏 马青 韦小瑜 李世军 《中国人兽共患病学报》 CAS CSCD 北大核心 2022年第12期1087-1092,共6页
目的了解贵州省2013-2021年羊种布鲁氏菌的分子流行病学特征,为贵州省布鲁氏菌病疫情防控提供科学依据。方法以贵州省疾病预防控制中心2013-2021年分离、鉴定和保存的78株羊种布鲁氏菌分离株为研究对象,运用BCSP31-PCR及AMOS-PCR技术对... 目的了解贵州省2013-2021年羊种布鲁氏菌的分子流行病学特征,为贵州省布鲁氏菌病疫情防控提供科学依据。方法以贵州省疾病预防控制中心2013-2021年分离、鉴定和保存的78株羊种布鲁氏菌分离株为研究对象,运用BCSP31-PCR及AMOS-PCR技术对分离株分别进行布鲁氏菌属及种的鉴定,运用多位点可变数目串联重复序列分析(MLVA-16)技术进行分型分析。对MLVA分型结果进行整理及聚类分析,了解贵州省羊种布鲁氏菌的MLVA型别分布及分子流行病学特征。结果78株羊种菌分离株经BCSP31-PCR技术均鉴定为布鲁氏菌属细菌,AMOS-PCR技术将其均鉴定为羊种布鲁氏菌,MLVA-8分型技术将其分为5种MLVA型,MLVA-16分型技术将其分为46种MLVA型,其中遵义市的MLVA-16基因型型别分布最多。基于MLVA-16分型数据聚类分析显示,78株分离株亲缘关系较近,被聚类为4个群;最小生成树图显示,78株分离株被分为8个遗传复合体和14个单体。结论贵州省羊种布鲁氏菌MLVA型别呈多样性,提示贵州省布病疫情的病原体可能存在多条传播链。 展开更多
关键词 羊种布鲁氏菌 MLVA 贵州省
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表面粗糙度非接触式测量系统的实现与应用 被引量:1
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作者 张倩 翁之钥 +2 位作者 应霞 王德琪 陈建钧 《计量与测试技术》 2018年第1期25-27,30,共4页
本文基于激光测距原理,通过物体表面形貌测量、数字图像处理和数据库技术的联合,提出了一种承载鞍表面粗糙度非接触式测量系统。文中介绍了该系统的硬件和软件两部分的实现过程,硬件主要包括检测装置及控制部分,软件部分主要涉及数据的... 本文基于激光测距原理,通过物体表面形貌测量、数字图像处理和数据库技术的联合,提出了一种承载鞍表面粗糙度非接触式测量系统。文中介绍了该系统的硬件和软件两部分的实现过程,硬件主要包括检测装置及控制部分,软件部分主要涉及数据的接收和承载鞍表面轮廓图的绘制,以得到承载鞍表面粗糙度值。该测量系统的应用,可显著提高承载鞍表面粗糙度测量的效率和精度。 展开更多
关键词 承载鞍 非接触式 粗糙度 点激光测量
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习近平新时代中国特色社会主义思想的理论逻辑
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作者 徐友龙 应霞 《观察与思考》 2023年第4期63-69,共7页
习近平新时代中国特色社会主义思想开辟了马克思主义新境界,是当代中国马克思主义、21世纪马克思主义。这一理论体系的逻辑主题是坚持和发展中国特色社会主义,逻辑核心是以人民为中心的发展思想,逻辑内涵是习近平关于新时代中国特色社... 习近平新时代中国特色社会主义思想开辟了马克思主义新境界,是当代中国马克思主义、21世纪马克思主义。这一理论体系的逻辑主题是坚持和发展中国特色社会主义,逻辑核心是以人民为中心的发展思想,逻辑内涵是习近平关于新时代中国特色社会主义经济、政治、文化、社会、生态文明等方面的重要论述,逻辑构境是推动构建人类命运共同体,逻辑本质是中国共产党的领导,逻辑方法是“六个必须坚持”。 展开更多
关键词 新时代 中国特色社会主义 新思想 理论逻辑 中国化时代化
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贵州省2株牛种布鲁氏菌分子流行病学特征分析 被引量:2
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作者 谭勤琴 王月 +5 位作者 刘英 营夏 杨幸贵 马青 胡勇 李世军 《中国人兽共患病学报》 CAS CSCD 北大核心 2023年第1期51-57,共7页
目的分析贵州省牛种布鲁氏菌分子流行病学特征,为牛种布鲁氏菌病疫情的防控提供科学依据。方法采用BCSP31-PCR及AMOS-PCR技术对经传统方法鉴定为牛种布鲁氏菌的2株分离株进行属/种复核,采用基于布鲁氏菌rpoB基因的单核苷酸多态性分析了... 目的分析贵州省牛种布鲁氏菌分子流行病学特征,为牛种布鲁氏菌病疫情的防控提供科学依据。方法采用BCSP31-PCR及AMOS-PCR技术对经传统方法鉴定为牛种布鲁氏菌的2株分离株进行属/种复核,采用基于布鲁氏菌rpoB基因的单核苷酸多态性分析了解其rpoB基因型,采用MLVA-16技术进行MLVA分型,了解其与国内牛种布鲁氏菌的聚类关系,采用MLST技术分析其序列型及其与国内菌株间的遗传进化关系。结果2株分离株经BCSP31-PCR和AMOS-PCR技术被鉴定为布鲁氏菌属细菌,未能明确其具体种型;其rpoB基因型相同;MLVA分型与新疆牛种布鲁氏菌共享同一MLVA-16型(4-5-3-12-2-2-3-1-6-43-8-5-5-5-3-3);MLST分析鉴定2株菌均为ST2型,最小间距图显示2株菌与布鲁氏菌属内的牛种布鲁氏菌遗传距离最近,属同一个遗传复合体。结论贵州省2株牛种布鲁氏菌分离株间的遗传距离近,与新疆牛种3型布鲁氏菌为同一MLVA型,提示分离株引起的感染可能为外省输入。 展开更多
关键词 牛种布鲁氏菌 RPOB MLVA MLST 贵州省
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2021年贵州省一起人间钩端螺旋体病疫情的分子流行病学调查分析
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作者 营夏 刘英 +8 位作者 姚光海 王铭 胡灿 周敬祝 王丹 杨幸贵 谭勤琴 胡勇 李世军 《中国人兽共患病学报》 CAS CSCD 北大核心 2023年第2期192-199,共8页
目的对2021年贵州省一起疑似钩端螺旋体病(钩体病)疫情开展病原学调查和分子流行病学分析,为病例的确诊和疫情防控提供科学依据。方法对收集的疑似钩体病例全血样本进行分离培养和致病性钩体特异性PCR检测,同时采用显微镜凝集试验(MAT)... 目的对2021年贵州省一起疑似钩端螺旋体病(钩体病)疫情开展病原学调查和分子流行病学分析,为病例的确诊和疫情防控提供科学依据。方法对收集的疑似钩体病例全血样本进行分离培养和致病性钩体特异性PCR检测,同时采用显微镜凝集试验(MAT)对病例血清进行钩体抗体检测。采用“夹夜法”捕捉疫区鼠类宿主动物,取肾脏进行钩体分离培养,采用致病性钩体特异性PCR进行检测,进一步采用血清群特异PCR进行鉴定。应用多位点序列分析(MLST)对本起疫情菌株进行基因分型,构建聚类分析图谱,分析其与国内菌株间的遗传和进化关系。结果3份疑似钩体病例样本经分离培养均为阴性,MAT试验分别为黄疸出血群、秋季群和爪哇群抗体阳性,经致病性钩体特异性PCR检出1例阳性。从鼠类宿主动物样本中分离出6株疑似钩体菌株,经鉴定分别为黄疸出血群(5/6)和秋季群菌株(1/6)。MLST分析显示,鼠类宿主动物携带的5株钩体为ST1型(83.33%),另1株为ST129型(16.67%)。聚类分析显示,鼠类宿主动物携带钩体与四川省、湖南省、江西省和贵州省往年菌株存在同一ST型。结论本起疫情是由致病性钩体感染引起,优势血清群和ST型与本省和周边省份往年分离株一致。秋季群菌株为贵州省近年来首次发现的钩体血清群,当地应加强监测以更好地预防和控制钩体病。 展开更多
关键词 钩端螺旋体病 钩端螺旋体 显微镜凝集试验 聚合酶链式反应 多位点序列分析
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Average iron isotopic compositions of the upper continental crust:constrained by loess from the Chinese Loess Plateau 被引量:3
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作者 yingzeng Gong ying xia +1 位作者 Fang Huang Huimin Yu 《Acta Geochimica》 EI CAS CSCD 2017年第2期125-131,共7页
Iron isotopic composition of the upper continental crust(UCC) is critical for understanding Fe mobilization and migration through the Earth. Because rocks exposed at Earth's surface have heterogeneous δ^(56)Fe, f... Iron isotopic composition of the upper continental crust(UCC) is critical for understanding Fe mobilization and migration through the Earth. Because rocks exposed at Earth's surface have heterogeneous δ^(56)Fe, finegrained clastic sediments can be used to estimate the average composition of UCC. In this study, we report δ^(56)Fe of loess-paleosol sequences from Yimaguan, Chinese Loess Plateau(CLP), to constrain the average Fe isotopic composition of UCC. The loess-paleosol sequences in this area formed in glacial-interglacial cycles and are characterized by varying degrees of weathering. Our data show that the loess-paleosol layers have extremely homogeneous Fe isotopic compositions with δ^(56)Fe ranging from 0.06‰ to 0.12‰, regardless of variations in the major element composition and weathering intensity. Our study indicates that since Fe isotopes are not significantly fractionated during loess deposition, the loess can be regarded as representative of UCC. It follows that the average δ^(56)Fe of UCC is 0.09‰± 0.03‰(2SD), consistent with previous estimates based on igneous rock data. 展开更多
关键词 Yimaguan LOESS Fe isotopes Uppercontinental crust
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Zircon saturation model in silicate melts:a review and update 被引量:2
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作者 Tongbin Shao ying xia +2 位作者 Xing Ding Yongfeng Cai Maoshuang Song 《Acta Geochimica》 EI CAS CSCD 2020年第3期387-403,共17页
Zircon stability in silicate melts-which can be quantitatively constrained by laboratory measurements of zircon saturation-is important for understanding the evolution of magma.Although the original zircon saturation ... Zircon stability in silicate melts-which can be quantitatively constrained by laboratory measurements of zircon saturation-is important for understanding the evolution of magma.Although the original zircon saturation model proposed by Watson and Harrison(Earth Planet Sci Lett 64(2):295-304,1983) is widely cited and has been updated recently,the three main models currently in use may generate large uncertainties due to extrapolation beyond their respective calibrated ranges.This paper reviews and updates zircon saturation models developed with temperature and compositional parameters.All available data on zircon saturation ranging in composition from mafic to silicic(and/or peralkaline to peraluminous)at temperatures from 750 to 1400℃ were collected to develop two refined models(1 and 2) that may be applied to the wider range of compositions.Model 1 is given by lnCZr(melt)=(14.297±0.308)+(0.964 ± 0.066).M-(11113±374)/r,and model 2 given by lnCZr(melt)=(18.99±0.423)-(1.069±0.102)·lnG-(12288±593)/T,where CZr(melt) is the Zr concentration of the melt in ppm and parameters M [=(Na+K+2 Ca)/(Al·Si)](cation ratios) and G [=(3·Al2 O3+SiO2)/(Na2-O+K2 O+CaO+MgO+FeO)](molar proportions)represent the melt composition.The errors are at one sigma,and T is the temperature in Kelvin.Before applying these models to natural rocks,it is necessary to ensure that the zircon used to date is crystallized from the host magmatic rock.Assessment of the application of both new and old models to natural rocks suggests that model 1 may be the best for magmatic temperature estimates of metaluminous to peraluminous rocks and that model 2 may be the best for estimating magmatic temperatures of alkaline to peralkaline rocks. 展开更多
关键词 Zircon Zircon saturation MODEL Silicate melt Mafic to silicic melts Peraluminous to peralkaline compositions Igneous rocks THERMOMETER
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Self‐training maximum classifier discrepancy for EEG emotion recognition 被引量:2
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作者 Xu Zhang Dengbing Huang +3 位作者 Hanyu Li Youjia Zhang ying xia Jinzhuo Liu 《CAAI Transactions on Intelligence Technology》 SCIE EI 2023年第4期1480-1491,共12页
Even with an unprecedented breakthrough of deep learning in electroencephalography(EEG),collecting adequate labelled samples is a critical problem due to laborious and time‐consuming labelling.Recent study proposed t... Even with an unprecedented breakthrough of deep learning in electroencephalography(EEG),collecting adequate labelled samples is a critical problem due to laborious and time‐consuming labelling.Recent study proposed to solve the limited label problem via domain adaptation methods.However,they mainly focus on reducing domain discrepancy without considering task‐specific decision boundaries,which may lead to feature distribution overmatching and therefore make it hard to match within a large domain gap completely.A novel self‐training maximum classifier discrepancy method for EEG classification is proposed in this study.The proposed approach detects samples from a new subject beyond the support of the existing source subjects by maximising the discrepancies between two classifiers'outputs.Besides,a self‐training method that uses unlabelled test data to fully use knowledge from the new subject and further reduce the domain gap is proposed.Finally,a 3D Cube that incorporates the spatial and frequency information of the EEG data to create input features of a Convolutional Neural Network(CNN)is constructed.Extensive experiments on SEED and SEED‐IV are conducted.The experimental evaluations exhibit that the proposed method can effectively deal with domain transfer problems and achieve better performance. 展开更多
关键词 artificial intelligence BIOINFORMATICS domain adaptation EEG neural network pattern classification
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Co-transplantation of macaque autologous Schwann cells and human embryonic nerve stem cells in treatment of macaque Parkinson's disease 被引量:1
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作者 ying xia Chengchuan Jiang +2 位作者 Zuowei Cao Keshan Shi Yang Wang 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2012年第1期7-14,共8页
Objective:To investigate the therapeutic effects of co-transplantation with Schwann cells(SCs) and human embryonic nerve stem cells(NSCs) on macaque Parkinson’s disease(PD).Methods: Macaque autologous SCs and h... Objective:To investigate the therapeutic effects of co-transplantation with Schwann cells(SCs) and human embryonic nerve stem cells(NSCs) on macaque Parkinson’s disease(PD).Methods: Macaque autologous SCs and human embryonic NSCs were adopled for the treatment of macaque PD.Results:Six months after transplantation,positron emission computerized tomography showed that <sup>18</sup>F-FP-β-CIT was significantly concentrated in the injured striatum in the cotransplanted group.Immunohistoehemieal staining of transplanted area tissue showed migration of tyrosine hydroxylase positive cells from the transplant area to the surrounding area was significantly increased in the co-transplanted group.Conclusions:Co-transplantation of SCs and NSCs could effectively cure PD in macaques.SCs harvested from the autologous peripheral nerves can avoid rejection and the ethics problems,so it is expected to be applied clinically. 展开更多
关键词 Schwann CELLS NERVE stem CELLS Parkinson’s disease CO-TRANSPLANTATION MACAQUE
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Radio map updated method based on subscriber locations in indoor WLAN localization 被引量:1
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作者 ying xia Zhongzhao Zhang Lin Ma 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2015年第6期1202-1209,共8页
With the rapid development of wireless local area network (WLAN) technology, an important target of indoor positioning systems is to improve the positioning accuracy while reducing the online calibration effort to o... With the rapid development of wireless local area network (WLAN) technology, an important target of indoor positioning systems is to improve the positioning accuracy while reducing the online calibration effort to overcome signal time-varying. A novel fingerprint positioning algorithm, known as the adaptive radio map with updated method based on hidden Markov model (HMM), is proposed. It is shown that by using a collection of user traces that can be cheaply obtained, the proposed algorithm can take advantage of these data to update the labeled calibration data to further improve the position estimation accuracy. This algorithm is a combination of machine learning, information gain theory and fingerprinting. By collecting data and testing the algorithm in a realistic indoor WLAN environment, the experiment results indicate that, compared with the widely used K nearest neighbor algorithm, the proposed algorithm can improve the positioning accuracy while greatly reduce the calibration effort. 展开更多
关键词 subscriber location wireless local area network(WLAN) positioning accuracy calibration effort hidden Markovmodel (HMM).
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The relationship between chronic PTSD,cortical volumetry and white matter microstructure among Australian combat veterans
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作者 Madeline Romaniuk ying xia +4 位作者 Gina Fisher Kerstin Pannek Jurgen Fripp Justine Evans Stephen Rose 《Military Medical Research》 SCIE CAS CSCD 2023年第3期313-324,共12页
Background:Posttraumatic stress disorder(PTSD)has been associated with volumetric and white matter microstructural changes among general and veteran populations.However,regions implicated have greatly varied and often... Background:Posttraumatic stress disorder(PTSD)has been associated with volumetric and white matter microstructural changes among general and veteran populations.However,regions implicated have greatly varied and often conflict between studies,potentially due to confounding comorbidities within samples.This study compared grey matter volume and white matter microstructure among Australian combat veterans with and without a lifetime diagnosis of PTSD,in a homogenous sample assessed for known confounding comorbidities.Methods:Sixty-eight male trauma-exposed veterans(16 PTSD-diagnosed;mean age 69 years)completed a battery of psychometric assessments and underwent magnetic resonance and diffusion tensor imaging.Analyses included tractbased spatial statistics,voxel-wise analyses,diffusion connectome-based group-wise analysis,and volumetric analysis.Results:Significantly smaller grey matter volumes were observed in the left prefrontal cortex(P=0.026),bilateral middle frontal gyrus(P=0.021),and left anterior insula(P=0.048)in the PTSD group compared to controls.Significant negative correlations were found between PTSD symptom severity and fractional anisotropy values in the left corticospinal tract(R^(2)=0.34,P=0.024)and left inferior cerebellar peduncle(R^(2)=0.62,P=0.016).No connectome-based differences in white matter properties were observed.Conclusions:Findings from this study reinforce reports of white matter alterations,as indicated by reduced fractional anisotropy values,in relation to PTSD symptom severity,as well as patterns of reduced volume in the prefrontal cortex.These results contribute to the developing profile of neuroanatomical differences uniquely attributable to veterans who suffer from chronic PTSD. 展开更多
关键词 Posttraumatic stress disorder(PTSD) VETERANS Diffusion tensor imaging Magnetic resonance imaging(MRI) Fractional anisotropy
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Improvement of Parkinsonian behavior with co-grafts of Schwann cells and neural stem cells in the rat
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作者 ying xia Chengchuan Jiang +3 位作者 Zhongliang Ding Yang Wang Bin Xu Linyin Feng 《Neural Regeneration Research》 SCIE CAS CSCD 2008年第1期5-9,共5页
BACKGROUND: Due to the lack of autograft transplant rejection, Schwann cells (SCs) can promote the proliferation of embryonic stem cells and the induction of dopaminergic neurons. Mesencephalic stem cells can be in... BACKGROUND: Due to the lack of autograft transplant rejection, Schwann cells (SCs) can promote the proliferation of embryonic stem cells and the induction of dopaminergic neurons. Mesencephalic stem cells can be induced to produce dopaminergic neurons. The therapeutic effects of co-grafts of SCs and neural stem cells (NSCs) deserves further study and verification in Parkinsonian animal models. OBJECTIVE: To investigate the effects of Schwann cells and mesencephalic NSC co-grafts in Parkinsonian animal models on animal behavior and histology. DESIGN: Randomized controlled experiment. SETTING: Fudan University; Institute of Neuroscience, Chinese Academy of Sciences. MATERIALS: The following animals were obtained from the Experimental Animal Center, Shanghai Institute for Biological Science, Chinese Academy of Sciences: 5 Sprague-Dawley rats, embryonic day (E) 13-16; 16 neonatal Sprague-Dawley rats, postnatal day 1-3; and 18 adult SD rats of both genders. Animal experimentation met animal ethical approval. METHODS: The experiment was performed at the Department of Anatomy, Histology and Embryology, Shanghai Medical Center, Fudan University from September 2005 to January 2007. The mesencephalic NSCs were obtained from the brains of SD rats at E 13-16, and SCs were harvested from the sciatic nerves of neonatal rats at day 1-3. Hemiparkinsonian rats (n =18) were selected for transplantation after estimating rotational behavior in response to apomorphine and were randomly assigned to three groups: control group, NSC group, and co-graft group. There were 6 rats in each group. Either phosphate buffered saline (PBS), NSCs, or SCs plus NSCs were transplanted into the right neostriatum of Parkinsonian rats, respectively. MAIN OUTCOME MEASURES: (1) Rotational behavior was induced by apomorphine (0.05 mg/kg, i.p.) 2, 4, 6, 8, and 10 weeks after transplantation, and the number of rotations were counted. (2) Differentiation and survival of dopaminergic neurons in the right neostriatum were quantified by tyrosine hydroxylase immunohistochemistry 10 weeks after grafting. RESULTS: All 18 Parkinsonian rats were included in the final analysis,without any loss. (1)Rotation behavior and turning: Compared with the control group, the percent of apomorphine-induced rotations were significantly decreased (P 〈 0.01 ) in both the NSC group and co-graft group. (2)Differentiation and survival of dopaminergic neurons in each group: TH-immunoreactive neurons were detected in the striatum of both the NSC group and co-graft group 10 weeks after transplantation. The neuronal volume, size of the nucleus, and neuronal numbers were larger in the co-graft group compared to the NSC group (P 〈 0.05). CONCLUSION: SC and NSC co-grafts not only improve Parkinsonian behavior in rats, but also improve the survival of NSCs. 展开更多
关键词 Schwann cells neural stem cells Parkinson's disease co-graft
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Semi-Supervised Clustering Fingerprint Positioning Algorithm Based on Distance Constraints
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作者 ying xia Zhongzhao Zhang +1 位作者 Lin Ma Yao Wang 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2015年第6期55-61,共7页
With the rapid development of WLAN( Wireless Local Area Network) technology,an important target of indoor positioning systems is to improve the positioning accuracy while reducing the online computation.In this paper,... With the rapid development of WLAN( Wireless Local Area Network) technology,an important target of indoor positioning systems is to improve the positioning accuracy while reducing the online computation.In this paper,it proposes a novel fingerprint positioning algorithm known as semi-supervised affinity propagation clustering based on distance function constraints. We show that by employing affinity propagation techniques,it is able to use a fractional labeled data to adjust similarity matrix of signal space to cluster reference points with high accuracy. The semi-supervised APC uses a combination of machine learning,clustering analysis and fingerprinting algorithm. By collecting data and testing our algorithm in a realistic indoor WLAN environment,the experimental results indicate that the proposed algorithm can improve positioning accuracy while reduce the online localization computation,as compared with the widely used K nearest neighbor and maximum likelihood estimation algorithms. 展开更多
关键词 wireless local area network(WLAN) SEMI-SUPERVISED similarity matrix CLUSTERING affinity propagation
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Isolation and identification of four pathogenic bacterial strains from edible snake(Elaphe carinata and Ptyas mucosus)farms with pneumonia in China
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作者 ying xia Siyu Long +2 位作者 Yuxuan Peng Songkang Qin Yaoqin Shen 《Animal Diseases》 2023年第1期56-61,共6页
This report describes an outbreak and treatment of pneumonia and enteritis in a snake farm with more than 3000 snakes containing Elaphe carinata(one-year-old)and Ptyas mucosus(three-month-old)seedlings in Huanggang,Hu... This report describes an outbreak and treatment of pneumonia and enteritis in a snake farm with more than 3000 snakes containing Elaphe carinata(one-year-old)and Ptyas mucosus(three-month-old)seedlings in Huanggang,Hubei,China.Gentamicin was used once in the early stage as treatment,administered orally with water or feed by owners,but mortality increased.Lobar pneumonia was confirmed by dissection and histopathology in infected snakes.Four main pathogenic bacteria were isolated and identified with culture and 16S rRNA sequencing:Staphylococcus sciuri,Salmonella enteritis,Vagococcus fluvialis and Providencia vermicola.Drug susceptibility tests were performed,and amikacin,gentamicin and cefitriaxone were chosen accordingly.After two rounds of treatment,the clinical signs for Elaphe carinata were under control,and the mortality was close to 0%after treatment.However,treatments for Ptyas mucosus seedlings did not work well,potentially because of poor administration technique and weak body condition. 展开更多
关键词 Staphylococcus sciuri Antimicrobial resistant Edible snakes DERMATITIS
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Acute andchronic cytotoxicity and genotoxicity induced by 2,6-dichloro-1,4-benzoquinone in NIH3T3 cells
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作者 ying xia xiao Wei 《广西医科大学学报》 CAS 2018年第3期281-285,共5页
Objective:This study aimed to evaluate the acute and chronic cytotoxicity and genotoxicity of 2,6-dichloro-1,4-benzoquinone(2,6-DCBQ).Methods:NIH3T3 cells were exposed to 2,6-DCBQ for 3 and 72 h,and relative cell viab... Objective:This study aimed to evaluate the acute and chronic cytotoxicity and genotoxicity of 2,6-dichloro-1,4-benzoquinone(2,6-DCBQ).Methods:NIH3T3 cells were exposed to 2,6-DCBQ for 3 and 72 h,and relative cell viability was calculated.NIH3T3 cells were treated with different concentrations of 2,6-DCBQ for 24 h.The solvent and positive controls were dimethyl sulfoxide(DMSO)and 0.5μmol/L ethylmethylsulfone,respectively.The values of Olive tail moment were measured by comet assay.NIH3T3 cells were then simultaneously treated with 5μg/mL cytochalasin B and different concentrations of 2,6-DCBQ.The solvent and positive controls were DMSO and 1μmol/L mitomycin C,respectively.Micronucleus rates were calculated after 48 h.Results:The half lethal doses of 2,6-DCBQ in NIH3T3 cells were 64.93μmol/L for 3 hand 13.46μmol/L for 72 h.The values of Olive tail moment in the 7.5 and 10μmol/L groups were significantly higher than those in the solvent control(P<0.05).Moreover,the micronucleus rates in the 10 and 15μmol/L groups were significantly higher than those in the solvent control(P <0.05).The results of comet assay and micronucleus test showed a dose-response relationship.Conclusion:2,6-DCBQ exhibited strong cytotoxicity and induced DNA and chromosomal damage in NIH3T3 cells. 展开更多
关键词 DRINKING water BENZOQUINONE CYTOTOXICITY GENOTOXICITY
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Development and Characterization of 35 SNP Markers in the Fat Greenling Hexagrammos otakii
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作者 xianggang Gao Weidong Liu +1 位作者 xiangbo Bao ying xia 《Open Journal of Marine Science》 2023年第1期1-6,共6页
Hexagrammos otakii is an important kind of economic fish species in East Asia. However, wild H. otakii resources have declined sharply in recent years as a result of human disturbance and habitat destruction. Thus, it... Hexagrammos otakii is an important kind of economic fish species in East Asia. However, wild H. otakii resources have declined sharply in recent years as a result of human disturbance and habitat destruction. Thus, it is crucial to protect the current resources of H. otakii. In this study, 35 novel single nucleotide polymorphism (SNP) markers were developed based on restriction-site associated DNA sequencing. The results showed that the observed heterozygosity and expected heterozygosity ranged from 0.1875 to 0.6562 and 0.2679 to 0.5079, respectively. The minor allele frequency ranged from 0.1875 to 0.4375. Polymorphic information content ranged from 0.229 to 0.375. Six SNPs were found to be deviated significantly from the HWE (P H. otakii. 展开更多
关键词 Hexagrammos otakii SNP RAD Genetic Diversity
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Modulation of starch synthesis in Arabidopsis via phytochrome B-mediated light signal transduction 被引量:2
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作者 Qingbiao Shi ying xia +8 位作者 Na Xue Qibin Wang Qing Tao Mingjing Li Di Xu xiaofei Wang Fanying Kong Haisen Zhang Gang Li 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2024年第5期973-985,共13页
Starch is a major storage carbohydrate in plants and is critical in crop yield and quality.Starch synthesis is intricately regulated by internal metabolic processes and external environmental cues;however,the precise ... Starch is a major storage carbohydrate in plants and is critical in crop yield and quality.Starch synthesis is intricately regulated by internal metabolic processes and external environmental cues;however,the precise molecular mechanisms governing this process remain largely unknown.In this study,we revealed that high red to far-red(high R:FR)light significantly induces the synthesis of leaf starch and the expression of synthesis-related genes,whereas low R:FR light suppress these processes.Arabidopsis phytochrome B(phyB),the primary R and FR photoreceptor,was identified as a critical positive regulator in this process.Downstream of phyB,basic leucine zipper transcription factor ELONGATED HYPOCOTYL5(HY5)was found to enhance starch synthesis,whereas the basic helix-loop-helix transcription factors PHYTOCHROME INTERACTING FACTORs(PIF3,PIF4,and PIF5)inhibit starch synthesis in Arabidopsis leaves.Notably,HY5 and PIFs directly compete for binding to a shared G-box cis-element in the promoter region of genes encoding starch synthases GBSS,SS3,and SS4,which leads to antagonistic regulation of their expression and,consequently,starch synthesis.Our findings highlight the vital role of phyB in enhancing starch synthesis by stabilizing HY5 and facilitating PIFs degradation under high R:FR light conditions.Conversely,under low R:FR light,PIFs predominantly inhibit starch synthesis.This study provides insight into the physiological and molecular functions of phyB and its downstream transcription factors HY5 and PIFs in starch synthesis regulation,shedding light on the regulatory mechanism by which plants synchronize dynamic light signals with metabolic cues to module starch synthesis. 展开更多
关键词 Arabidopsis thaliana HY5 light signal PHYB PIFs starch synthesis
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