The osteoclastic activity in apical distal region of molar mesial roots affects orthodontic tooth movement and root resorption in rats

The utilization of optimal orthodontic force is crucial to prevent undesirable side effects and ensure efficient tooth movement during orthodontic treatment.However,the sensitivity of existing detection techniques is not sufficient,and the criteria for evaluating optimal force have not been yet established.Here,by employing 3D finite element analysis methodology,we found that the apical distal region(A-D region)of mesial roots is particularly sensitive to orthodontic force in rats.Tartrate-resistant acidic phosphatase(TRAP)-positive osteoclasts began accumulating in the A-D region under the force of 40 grams(g),leading to alveolar bone resorption and tooth movement.When the force reached 80 g,TRAP-positive osteoclasts started appearing on the root surface in the A-D region.Additionally,micro-computed tomography revealed a significant root resorption at 80 g.Notably,the A-D region was identified as a major contributor to whole root resorption.It was determined that 40 g is the minimum effective force for tooth movement with minimal side effects according to the analysis of tooth movement,inclination,and hyalinization.These findings suggest that the A-D region with its changes on the root surface is an important consideration and sensitive indicator when evaluating orthodontic forces for a rat model.Collectively,our investigations into this region would aid in offering valuable implications for preventing and minimizing root resorption during patients’orthodontic treatment.

1例化脓性脑膜炎伴肾功能不全小儿患者的治疗分析与药学监护

目的探讨化脓性脑膜炎伴肾功能不全小儿患者的药物治疗及开展药学监护的方法。方法临床药师利用治疗药物监测手段,与临床医师共同制订个体化给药方案。结果临床药师通过实施药学监护,提供个体化药学服务,提高了临床治疗效果。结论临床药师应当深入临床,积极参与临床实践,结合患者病情,发挥药学专业优势,协同临床医师优化给药方案。

社会网络中心性与公司捐赠

以往关于公司捐赠的研究主要从单个公司的角度研究捐赠的影响因素,忽略了公司间的互动促进相关价值信息传递如何影响公司捐赠。社会网络理论表明,公司之间的社会网络是重要的信息传递渠道,对公司决策产生重要影响。本文以2008-2016年上市公司为研究样本,基于高管和董事兼职关系构建一个新的公司网络中心性面板数据集,考察网络中心性对公司捐赠的影响。实证结果表明网络中心性越高,公司捐赠水平越高。本文运用准自然实验方法和工具变量方法缓解内生性问题,结果依然稳健。横截面差异的检验结果表明网络中心性对公司捐赠的影响在市场地位低、市场化程度低的公司中显著更强。研究结果对于理解公司捐赠的决策制定具有参考价值。

灵芝蛋白多糖抗人胰淀素诱导β细胞凋亡的实验研究

目的探讨灵芝蛋白多糖(FYGL)对人胰淀素诱导的胰岛β细胞凋亡的保护作用。方法 FYGL对正常INS-1胰岛细胞的毒性实验;FYGL对IAPP干预的INS-1细胞活性的影响;FYGL对IAPP干预的INS-1细胞凋亡及内质网应激相关因子的表达影响。结果 FYGL各剂量均未对正常INS-1细胞产生毒性作用;XTT结果显示:与CON组比较,25u M IAPP可明显降低细胞活性,差异有统计学意义(P <0.01),与MOD组比较,各剂量组FYGL可提高细胞活性,且呈剂量依赖性,差异有统计学意义(均P <0.05);real-time PCR结果:与CON组比较,MOD组中caspase3、CHOP及JNK1在分子水平表达均显著上升;与CON组比较、MOD组中bcl-2在分子水平表达均显著下降,差异有统计学意义(均P <0.01)。结论 FYGL呈剂量依赖性抑制h IAPP诱导INS-1胰岛细胞凋亡,可能通过影响内质网应激CHOP、JNK信号通路而抑制h IAPP诱导INS-1细胞凋亡。

床边教学法在助产学教学中的应用

目的探讨床边教学法在助产学教学中的应用效果。方法便利选取浙江中医药大学2017级助产专业学生(62名)为研究对象,采用床边教学法,将助产学中的理论及实验课内容融入到临床实际当中进行教学。教学结束后了解学生的参与程度,应用教学效果问卷评价床边教学的效果,并将2017级学生案例分析成绩与2016级进行比较。结果学生课堂发言率为87.1%,提问率为53.2%;2017级学生期末案例分析成绩优于2016级学生(P<0.05)。2017级所有学生认为授课形式新颖,有创新性;96.8%的学生认为授课内容更贴近临床;95.2%的学生认为师生关系更为融洽;92.0%的学生认为对今后临床工作有帮助,有必要继续开展。结论床边教学法可提升助产专业学生的课堂参与度,有效提高教学效果和教学质量。

伏诺拉生与阿莫西林二联方案根除幽门螺杆菌的真实世界研究

目的 探讨常规剂量伏诺拉生联合常规剂量阿莫西林二联方案用于幽门螺杆菌(HP)感染初次治疗患者的疗效、安全性及经济性。方法 采用前瞻性队列研究方法,根据纳排标准纳入2021年7月至2022年7月在成都新华医院消化内科门诊诊断为HP感染且初次接受治疗的患者,根据患者用药意愿分别接受伏诺拉生+阿莫西林二联方案(即VA组,富马酸伏诺拉生片20 mg,每天1次+阿莫西林胶囊1.0 g,每天2次,疗程14 d)和经典的含铋剂四联方案(即LJAF组,雷贝拉唑肠溶片20 mg,每天2次+胶体果胶铋胶囊200 mg,每天2次+阿莫西林胶囊1.0 g,每天2次+呋喃唑酮片100 mg,每天2次,疗程14 d)。治疗结束4周后,采用意向治疗(ITT)、改良的意向治疗(MITT)、按方案治疗(PP)分析,比较两组患者的HP清除率;记录其不良反应发生情况,并进行经济性评价。结果 VA组58例患者中,55例完成试验,2例失访,1例因皮疹退出;LJAF组62例患者中,57例完成试验,3例失访,2例因皮疹退出。ITT、MITT、PP分析结果显示,VA组患者的HP根除率分别为86.2%、89.3%、90.9%,LJAF组患者的HP根除率分别为87.1%、91.5%、94.7%,组间比较差异均无统计学意义(P>0.05)。VA组、LJAF组患者的不良反应发生率分别为6.9%、14.5%,组间比较差异亦无统计学意义(P>0.05)。最小成本分析结果显示,VA组患者的治疗总成本为340.9元,低于LJAF组的373.5元。结论 常规剂量伏诺拉生联合常规剂量阿莫西林二联方案用于HP感染初次治疗患者的疗效和安全性与经典的含铋剂四联方案相当,且总成本更低。

Association Between Homocysteine Level and Methylenetetrahydrofolate Reductase Gene Polymorphisms in Type 2 Diabetes Accompanied by Dyslipidemia

Objective To investigate the association between total homocysteine(tHcy)level in plasma and methylenetetrahydrofblate reductase(MTHFR)C677T and A1298C genetic polymorphisms in a Chinese Han nationality population with type 2 diabetes mellitus(T2DM)accompanied by dyslipidemia.Methods This case-control study enrolled T2DM patients with dyslipidemia and without dyslipidemia respectively.Sanger dideoxy-mediated chain-termination method was used to detect the gene polymorphisms of MTHFR C677T and A1298C.Plasma tHcy and lipid levels were measured as well.The genotype frequency and allele frequency between the dyslipidemia and non-dyslipidemia groups were compared by using Chi-square test.Plasma tHcy level ofT2DM patients who carried the different genotypes was compared by Student's t test.Results Finally,82 T2DM patients with dyslipidemia and 94 ones without dyslipidemia were included in this study.There was a significant correlation between tHcy level and MTHFR C677T gene polymorphism inT2DM patients(t=2.27,P=0.02).Moreover,the plasma tHcy level in the dyslipidemia patients who carried MTHFR 677TT genotype was significantly higher than that in those with CT+CC genotype(13.62+6.97 vs.10.95+3.62pmol/L,t=2.2O,P=0.03);while for patients without dyslipidemia,comparison of the tHcy level between those who carried the above two alleles showed no significantly difference(13.34±6.03 vs.12.04±5.09μmol/L,t=1.08,P=0.29).Conclusion MTHFR 677TT genotype might associate with higher tHcy level in T2DM patients with dyslipidemia.

疏肝理气方改善肝郁脾虚型慢性乙型肝炎肝功能、肝纤维化的作用以及对肠道菌群的影响

目的探讨疏肝理气方治疗肝郁脾虚型慢性乙型肝炎患者的疗效以及对肠道菌群的影响。方法选取2017年1月—2019年1月医院收治慢性乙型肝炎肝纤维化患者80例,以数字表法随机分为观察组和对照组各40例。对照组给予恩替卡韦片治疗,口服,0.5 mg/次,1次/d。观察组在对照组治疗基础上给予疏肝理气方内服治疗,每日1剂,2次/d。两组疗程均为6个月。比较两组患者的肝郁脾虚证症状评分、肝功能、肝纤维化、临床疗效以及双歧杆菌、乳酸杆菌、大肠杆菌、肠球菌水平。结果治疗6个月后,观察组患者肝郁脾虚证症状评分、肝功能指标[天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、总胆红素(TBIL)、γ-谷氨酸酰基转移酶(GGT)]水平以及肝纤维化指标[透明质酸(HA)、Ⅲ型前胶原(PCⅢ)、Ⅳ型胶原(Ⅳ-C)、层黏蛋白(LN)]水平明显低于对照组(P<0.01);治疗6个月后,观察组总有效率明显高于对照组(P<0.05);治疗6个月后,观察患者的双歧杆菌、乳酸杆菌水平明显高于对照组,大肠杆菌、肠球菌明显低于对照组(P<0.01)。结论在常规西医治疗基础上,疏肝理气方治疗肝郁脾虚型慢性乙型肝炎患者的疗效明显,且可改善患者的肠道菌群。

The Olig family affects central nervous system development and disease

Neural cell differentiation and maturation is a critical step during central nervous system devel-opment. The oligodendrocyte transcription family （Olig family） is known to be an important factor in regulating neural cell differentiation. Because of this, the Olig family also affects acute and chronic central nervous system diseases, including brain injury, multiple sclerosis, and even gliomas. Improved understanding about the functions of the Olig family in central nervous system development and disease will greatly aid novel breakthroughs in central nervous system diseases. This review investigates the role of the Olig family in central nervous system develop- ment and related diseases.

Methylenetetrahydrofolate Reductase Gene Polymorphism C677T is Associated with Increased Risk of Coronary Heart Disease in Chinese Type 2 Diabetic Patients

Objective Chronic cardiovascular diseases induced by long-term poor blood glucose control are the main cause of death in patients with type 2 diabetes mellitus(T2DM).Previous researches report that methylenetetrahydrofolate reductase gene(MTHFR)polymorphisms might influence the occurrence of coronary heart disease(CHD)in T2DM patients.The purpose of this study was to evaluate whether MTHFR C677T and A1298C mutations are associated with the risk of CHD inT2DM patients.Methods A total of 197 subjects with T2DM were studied,of which 95 patients with CHD.The genotypes of MTHFR C677T and A1298C were analyzed by using dideoxy chain-termination method,and compared between patients with CHD and those without CHD.Results We found that the frequency of the 677T allele was significantly higher in T2DM patients with CHD than those without CHD(P=0.011).However,there was no significant difference in any of the examined haplotypes between T2DM patients with and without CHD.Furthermore,the 677T allele was associated with a higher risk of CHD development in diabetic patients with lower homocysteine(Hey)levels(≤15μmol/L)(P=0.006),while no effect of MTHFR gene polymorphism on the incidence of CHD was found in patients with higher Hey levels(>15 μmol/L)(P=0.491).Conclusion The MTHFR C677T gene polymorphism is associated with the risk of CHD of diabetic patients and could be used as an effective marker for CHD in Chinese diabetic populations with normal Hey levels.

A Vaccine Based on the Receptor-Binding Domain of the Spike Protein Expressed in Glycoengineered Pichia pastoris Targeting SARS-CoV-2 Stimulates Neutralizing and Protective Antibody Responses

In 2020 and 2021,severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),a novel coronavirus,caused a global pandemic.Vaccines are expected to reduce the pressure of prevention and control,and have become the most effective strategy to solve the pandemic crisis.SARS-CoV-2 infects the host by binding to the cellular receptor angiotensin converting enzyme 2(ACE2)via the receptor-binding domain(RBD)of the surface spike(S)glycoprotein.In this study,a candidate vaccine based on a RBD recombinant subunit was prepared by means of a novel glycoengineered yeast Pichia pastoris expression system with characteristics of glycosylation modification similar to those of mammalian cells.The candidate vaccine effectively stimulated mice to produce high-titer anti-RBD specific antibody.Furthermore,the specific antibody titer and virus-neutralizing antibody(NAb)titer induced by the vaccine were increased significantly by the combination of the double adjuvants Al(OH)_(3) and CpG.Our results showed that the virus-NAb lasted for more than six months in mice.To summarize,we have obtained a SARS-CoV-2 vaccine based on the RBD of the S glycoprotein expressed in glycoengineered Pichia pastoris,which stimulates neutralizing and protective antibody responses.A technical route for fucose-free complex-type N-glycosylation modified recombinant subunit vaccine preparation has been established.

基于微信平台的团队培训对产科新入职护士能力及护理质量的影响研究

目的分析基于微信平台的学习型团队培训对产科新入职护士核心能力、岗位胜任能力及护理质量的影响,为新入职护士的培训提供参考。方法选取2017年7月至2020年12月我院产科新入职护士64人作为研究对象,其中2017年7月至2019年3月入职的护士为对照组,2019年4月至2020年12月入职的护士为研究组,每组各32例。对照组采用常规培训,观察组采用基于微信平台的学习型团队培训。比较两组护士产科理论知识及操作技能掌握情况、核心能力、岗位胜任能力、护理质量、护理培训模式满意度。结果培训后,两组核心能力、岗位胜任能力各方面评分及总分、理论知识及操作技能考试成绩均高于培训前(p<0.05),且观察组高于对照组(p<0.05);观察组护理投诉、护理差错、医疗纠纷发生率均低于对照组(p<0.05),对护理培训模式的满意度高于对照组(p<0.05)。结论基于微信平台的学习型团队培训有利于产科新入职护士对理论知识及操作技能的掌握,可有效提高其核心能力、岗位胜任能力、护理质量及培训满意度。

Identifying the dominant transport mechanism in single nanoscale pores and 3D nanoporous media

Gas transport mechanisms can be categorized into viscous flow and mass diffusion,both of which may coexist in a porous media with multiscale pore sizes.To determine the dominant transport mechanism and its contribution to gas transport capacity,the gas viscous flow and mass diffusion processes are analyzed in single nanoscale pores via a theoretical method,and are simulated in 3D nanoporous media via pore-scale lattice Boltzmann methods.The apparent permeability from the viscous flow and apparent diffusivity from the mass diffusion are estimated.A dimensionless parameter,i.e.,the diffusion-flow ratio,is proposed to evaluate the dominant transport mechanism,which is a function of the apparent permeability,apparent diffusivity,bulk dynamic viscosity,and working pressure.The results show that the apparent permeability increases by approximately two orders of magnitude when the average Knudsen number(Kn_(avg))of the nanoporous media or Knudsen number(Kn)of single nanoscale pores increases from 0.1 to 10.Under the same conditions,the increment in the apparent diffusivity is only approximately one order of magnitude.When Kn<0.01,the apparent permeability has a lower bound(i.e.,absolute permeability).When Kn>10,the apparent diffusivity has an upper bound(i.e.,Knudsen diffusivity).The dominant transport mechanism in single nanoscale pores is the viscous flow for 0.01<Kn<100,where the maximum diffusion-flow ratio is less than one.In nanoporous media,the dominant transport relies heavily on Kn_(avg) and the structural parameters.For nanoporous media with the pore throat diameter of 3 nm,Kn_(avg)=0.2 is the critical point,above which the mass diffusion is dominant;otherwise,the viscous flow is dominant.As Kn_(avg) increases to 3.4,the mass diffusion is overwhelming,with the maximum diffusion-flow ratio reaching ~4.

树莓中鞣花酸的提取及生物活性研究进展

鞣花酸为树莓的主要活性物质之一,存在于树莓的枝、叶、果中,因具有良好的抗氧化、抗癌、抑菌消炎等作用,受到了国内外学者的关注。基于鞣花酸较高的开发和利用价值,本文在前人研究的基础上,对近年来树莓中鞣花酸的提取方法进行了综述,同时总结了鞣花酸的生物活性,为树莓鞣花酸的进一步开发提供参考。

孕妇静观分娩训练体验的质性研究

目的探讨孕妇在静观分娩训练中的真实体验,为优化妇幼保健服务提供依据和借鉴。方法采用质性研究中诠释现象学的理论方法,对12名接受过静观分娩训练的孕妇进行半结构式深入访谈,访谈资料应用Colaizzi分析法分析整理。结果接受过静观分娩训练的孕妇体验包括学会关注当下、自我调节力改善、同情和感恩、孕期生活质量提高4大主题。结论静观分娩训练有助于围生期孕产妇的健康促进,但促进水平还需进一步定量研究。

发育性髋关节发育不良的早期Pavlik吊带治疗进展

发育性髋关节发育不良是一种儿童常见的下肢畸形,错过早期最佳治疗时间,预后较差。早期(小于6个月)通过体检筛查及超声检查可以诊断,有经验的医师可采用Pavlik吊带进行规范化治疗,同时对患儿家庭进行应用护理教育和有效沟通,常能获得良好预后。早期应用Pavlik吊带治疗发育性髋关节发育不良具有轻便、经济、护理方便等优势,值得在社区推广应用。

2015年北京市白纸坊地区健康素养现状分析

目的:了解北京市西城区白纸坊地区居民健康素养现状,为今后的健康促进工作提供科学依据。方法:采取多阶段分层整群随机抽样方法,2015年在白纸坊地区抽取231例进行健康素养问卷调查。结果:北京市西城区白纸坊地区居民具备健康素养的比例13.4%,具备基本知识理念、健康生活方式与行为和基本技能三方面素养的比例分别是16.9%、10.0%、19.0%。6个维度的健康素养水平分别为安全与急救(71.0%)、科学健康观(23.8%)、基本医疗(16.5%)、慢性病防治(14.7%)、健康信息(16.0%)和传染病防治(7.8%)。结论:研究对象中具备健康素养水平的比例比较低,需在该人群中大力开展健康促进工作,提高健康素养水平。

Solitary intraosseous neurofibroma in the mandible mimicking a cystic lesion:A case report and review of literature

BACKGROUND Neurofibromas are benign tumors of a neurogenic origin.If these tumors occur without any other signs of neurofibromatosis,they are classified as isolated neurofibromas.Neurofibromas in the oral cavity mostly occur within soft tissues,indicating that solitary intraosseous neurofibromas in the mandible are rare.Due to the absence of specific clinical manifestations,early diagnosis and treatment of these tumors are difficult to achieve.CASE SUMMARY A 37-year-old female patient visited our hospital due to numbness and swelling of the gums in the right lower molar area that had persisted for half a month.The patient’s overall condition and intraoral examination revealed no significant abnormalities.She was initially diagnosed with a cystic lesion in the right mandible.However,after a more thorough examination,the final pathological diagnosis was confirmed to be neurofibroma.Complete tumor resection and partial removal of the right inferior alveolar nerve were performed.As of writing this report,there have been no signs of tumor recurrence for nine months following the surgery.CONCLUSION This case report discusses the key features that are useful for differentiating solitary intraosseous neurofibromas from other cystic lesions.

Research Advance on the Relationship between Wee1 and Tumor Genesis and Progression

In the process of biological genetic information transmission,complete and correct genetic information can make cell mitosis proceed normally.In the development of most tumor cells,G2/M cell cycle checkpoint becomes the key checkpoint in the process of mitosis due to the lack of G1/S cell cycle checkpoint,which mainly depends on the abnormal DNA information blocked by Wee1 protein kinase in G2 phase to enter M phase and prolong the time of G2 phase to complete DNA sequencing So that the normal genetic information can be passed on.Wee1 protein kinase expression is significantly increased in most tumor cells,making it a potential target for tumor therapy.

Abound Hepatic Mitosis:Unusual Morphology in the Intrahepatic Cholelithiasis Patient

To explore the clinicopathological features of abound mitosis of the hepatocytes in intrahepatic cholelithiasis.The clinicopathological data of one case diagnosed as intrahepatic cholelithiasis was collected from Yantai Yuhuangding Hospital and the clinicopathological characters were discussed.A 68-year-old man suffered from the pain in the right upper quadrant and radiology showed multiple stones in the gallbladder and left liver.The images suggested intrahepatic cholelithiasis.The patient received gallbladder and partial hepatectomy.A large number of mitosis was observed and twelve nuclear fissions were found under high magnification,even in some area pathological nuclear fission could be observed in morphology.On the basis of detection in laboratory,the diagnosis of intrahepatic cholelithiasis was made.The patient did not receive any therapy after surgery.The patient was in a good condition after 18 months follow-up.Increased number of hepatic mitosis might be due to the stimulation from stones,hepatic biliary or secondary inflammatory.High index of proliferation should be prevented from the potential misdiagnosis of hepatic tumor.