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Evaluation of COC183B2 antibody targeting ovarian cancer by near-infrared fluorescence imaging 被引量:4
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作者 Chen zhang Xinyu Ling +10 位作者 Yanxiu Guo Cunzhong Yuan Hongyan Cheng Xue Ye Ruiqiong Ma yinli zhang Yi Li Xiaohong Chang Beihua Kong Tao Liu Heng Cui 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 2019年第4期673-685,共13页
Objective: To evaluate the imaging potential of a novel near-infrared(NIR) probe conjugated to COC183 B2 monoclonal antibodies(MAb) in ovarian cancer(OC).Methods: The expression of OC183 B2 antigen in OC was determine... Objective: To evaluate the imaging potential of a novel near-infrared(NIR) probe conjugated to COC183 B2 monoclonal antibodies(MAb) in ovarian cancer(OC).Methods: The expression of OC183 B2 antigen in OC was determined by immunohistochemical(IHC) staining using tissue microarrays with the H-score system and immunofluorescence(IF) staining of tumor cell lines.Imaging probes with the NIR fluorescent dye cyanine 7(Cy7) conjugated to COC183 B2 Mab were chemically engineered. OC183 B2-positive human OC cells(SKOV3-Luc) were injected subcutaneously into BALB/c nude mice. Bioluminescent imaging(BLI) was performed to detect tumor location and growth. COC183 B2-Cy7 at 1.1,3.3, 10, or 30 μg were used for in vivo fluorescence imaging, and phosphate-buffered saline(PBS), free Cy7 dye and mouse isotype immunoglobulin G(IgG)-Cy7(delivered at the same doses as COC183 B2-Cy7) were used as controls.Results: The expression of OC183 B2 with a high H-score was more prevalent in OC tissue than fallopian tube(FT) tissue. Among 417 OC patients, the expression of OC183 B2 was significantly correlated with the histological subtype, histological grade, residual tumor size, relapse state and survival status. IF staining demonstrated that COC183 B2 specifically expressed in SKOV3 cells but not HeLa cells. In vivo NIR fluorescence imaging indicated that COC183 B2-Cy7 was mainly distributed in the xenograft and liver with optimal tumor-to-background(T/B)ratios in the xenograft at 30 μg dose. The highest fluorescent signals in the tumor were observed at 96 h postinjection(hpi). Ex vivo fluorescence imaging revealed the fluorescent signals mainly from the tumor and liver. IHC analysis confirmed that xenografts were OC183 B2 positive.Conclusions: COC183 B2 is a good candidate for NIR fluorescence imaging and imaging-guided surgery in OC. 展开更多
关键词 COC183B2 ANTIBODY NEAR-INFRARED FLUORESCENCE imaging OVARIAN CANCER
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Recent applications of radical cascade reaction in the synthesis of functionalized 1-indenones 被引量:2
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作者 yinli zhang Kai Sun +3 位作者 Qiyan Lv Xiaolan Chen Lingbo Qua Bing Yu 《Chinese Chemical Letters》 SCIE CAS CSCD 2019年第7期1361-1368,共8页
1-Indenones are important scaffolds in natural products, biologically active molecules as well as functional materials. Recently, radical cascade cyclization has emerged as an efficient and powerful strategy for the c... 1-Indenones are important scaffolds in natural products, biologically active molecules as well as functional materials. Recently, radical cascade cyclization has emerged as an efficient and powerful strategy for the construction of valuable and versatile functionalized 1-indenones. In this review, the recent advances of this rapidly growing area were summarized. The selected examples have been classified according to the type of reaction substrates such as 1,3-diarylpropynones, 2-alkynylbenzonitriles, arylpropynols, 1,5-enynes and 2-alkynylated bromocinnamates. 展开更多
关键词 RADICAL CASCADE reaction 1 3-Diarylpropynones 2-Alkynylbenzonitriles Arylpropynols 1-Indenones
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