Maintaining chromosome euploidy in zebrafish embryonic cells is challenging because of the degradation of genomic integrity during cell passaging.In this study,we report the derivation of zebrafish cell lines from sin...Maintaining chromosome euploidy in zebrafish embryonic cells is challenging because of the degradation of genomic integrity during cell passaging.In this study,we report the derivation of zebrafish cell lines from single blastomeres.These cell lines have a stable chromosome status attributed to BMP4 and exhibit continuous proliferation in vitro.Twenty zebrafish cell lines are successfully established from single blastomeres.Single-cell transcriptome sequencing analysis confirms the fidelity of gene expression profiles throughout long-term culturing of at least 45 passages.The long-term cultured cells are specialized into epithelial cells,exhibiting similar expression patterns validated by integrative transcriptomic analysis.Overall,this work provides a protocol for establishing zebrafish cell lines from single blastomeres,which can serve as valuable tools for in vitro investigations of epithelial cell dynamics in terms of lifeedeath balance and cell fate determination during normal homeostasis.展开更多
The first long-term cultured haploid embryonic stem cell(ESC)line was established from medaka fish,which represented a breakthrough in vertebrates(Yi et al.,2009).Subsequently,haploid ESC lines were established from m...The first long-term cultured haploid embryonic stem cell(ESC)line was established from medaka fish,which represented a breakthrough in vertebrates(Yi et al.,2009).Subsequently,haploid ESC lines were established from mammals and used for genetic analysis and complex genetic manipulation(Elling et al.,2011;Yang et al.,2012).The diploidization of haploid cells is an interesting biological phenomenon and has been widely studied,but the mechanism of diploidization has not yet been fully resolved(Sun et al.,2020).The zebrafish is an excellent vertebrate animal model used in biological studies(Huang et al.,2022).Researchers have isolated many cell lines from zebrafish,such as the zebrafish fibroblast line ZF4(Driever and Rangini,1993).However,there have been no reports regarding stable haploid cell lines of zebrafish,and whether diploidization occurs in zebrafish is unclear.展开更多
基金supported by the National Key Research and Development Program of China(2018YFA0801003 and 2019YFA0109900)the Center for Excellence in Molecular Cell Science(2021DF06)supported by the Shanghai Municipal Science and Technology Major Project.
文摘Maintaining chromosome euploidy in zebrafish embryonic cells is challenging because of the degradation of genomic integrity during cell passaging.In this study,we report the derivation of zebrafish cell lines from single blastomeres.These cell lines have a stable chromosome status attributed to BMP4 and exhibit continuous proliferation in vitro.Twenty zebrafish cell lines are successfully established from single blastomeres.Single-cell transcriptome sequencing analysis confirms the fidelity of gene expression profiles throughout long-term culturing of at least 45 passages.The long-term cultured cells are specialized into epithelial cells,exhibiting similar expression patterns validated by integrative transcriptomic analysis.Overall,this work provides a protocol for establishing zebrafish cell lines from single blastomeres,which can serve as valuable tools for in vitro investigations of epithelial cell dynamics in terms of lifeedeath balance and cell fate determination during normal homeostasis.
基金supported by the National Key Research and Development Program of China(2019YFA0109900 and 2018YFA0801003)CAS Center for Excellence in Molecular Cell Science(2021DF06)Shanghai Municipal Science and Technology Major Project.
文摘The first long-term cultured haploid embryonic stem cell(ESC)line was established from medaka fish,which represented a breakthrough in vertebrates(Yi et al.,2009).Subsequently,haploid ESC lines were established from mammals and used for genetic analysis and complex genetic manipulation(Elling et al.,2011;Yang et al.,2012).The diploidization of haploid cells is an interesting biological phenomenon and has been widely studied,but the mechanism of diploidization has not yet been fully resolved(Sun et al.,2020).The zebrafish is an excellent vertebrate animal model used in biological studies(Huang et al.,2022).Researchers have isolated many cell lines from zebrafish,such as the zebrafish fibroblast line ZF4(Driever and Rangini,1993).However,there have been no reports regarding stable haploid cell lines of zebrafish,and whether diploidization occurs in zebrafish is unclear.
