Simultaneous Degradation, Dehalogenation, and Detoxification of Halogenated Antibiotics by Carbon Dioxide Radical Anions

Despite the extensive application of advanced oxidation processes(AOPs)in water treatment,the efficiency of AOPs in eliminating various emerging contaminants such as halogenated antibiotics is constrained by a number of factors.Halogen moieties exhibit strong resistance to oxidative radicals,affecting the dehalogenation and detoxification efficiencies.To address these limitations of AOPs,advanced reduction processes(ARPs)have been proposed.Herein,a novel nucleophilic reductant—namely,the carbon dioxide radical anion(CO_(2)^(·-))—is introduced for the simultaneous degradation,dehalogenation,and detoxification of florfenicol(FF),a typical halogenated antibiotic.The results demonstrate that FF is completely eliminated by CO_(2)^(·-),with approximately 100%of Cland 46%of Freleased after 120 min of treatment.Simultaneous detoxification is observed,which exhibits a linear response to the release of free inorganic halogen ions(R^(2)=0.97,p<0.01).The formation of halogen-free products is the primary reason for the superior detoxification performance of this method,in comparison with conventional hydroxyl-radical-based AOPs.Products identification and density functional theory(DFT)calculations reveal the underlying dehalogenation mechanism,in which the chlorine moiety of FF is more susceptible than other moieties to nucleophilic attack by CO_(2)^(·-).Moreover,CO_(2)^(·-)-based ARPs exhibit superior dehalogenation efficiencies(>75%)in degrading a series of halogenated antibiotics,including chloramphenicol(CAP),thiamphenicol(THA),diclofenac(DLF),triclosan(TCS),and ciprofloxacin(CIP).The system shows high tolerance to the pH of the solution and the presence of natural water constituents,and demonstrates an excellent degradation performance in actual groundwater,indicating the strong application potential of CO_(2)^(·-)-based ARPs in real life.Overall,this study elucidates the feasibility of CO_(2)^(·-)for the simultaneous degradation,dehalogenation,and detoxification of halogenated antibiotics and provides a promising method for their regulation during water or wastewater treatment.

Umami taste components in chicken-spices blends and potential effect of aroma on umami taste intensity

In this study,umami taste intensity(UTI)and umami taste components in chicken breast(CB)and chickenspices blends were characterized using sensory and instrumental analysis.Our main objective was to assess the aroma-umami taste interactions in different food matrices and reconcile the aroma-taste perception to assist future product development.The impact of key aroma,including vegetable-note"2-pentylfuran",meaty"methional",green"hexanal",and spicy-note-estragole and caryophyllene"on UTI was evaluated in monosodium glutamate and chicken extract.We found that spices significantly decreased UTI and umami taste components in CB.Interestingly,the perceptually similar odorants and tastants exhibited the potential to enhance UTI in food matrices.Methional was able to increase the UTI,whereas spicy and green-note components could reduce the UTI significantly.This information would be valuable to food engineers and formulators in aroma selection to control the UTI perceived by consumers,thus,improving the quality and acceptability of the chicken products.

Hydroxysafflor yellow A induced ferroptosis of Osteosarcoma cancer cells by HIF-1α/HK2 and SLC7A11 pathway

Osteosarcoma is a very serious primary bone cancer with a high death rate and a dismal prognosis.Since there is no permanent therapy for this condition,it is necessary to develop a cure.Therefore,this investigation was carried out to assess the impacts and biological functions of hydroxysafflor yellow A(HYSA)in osteosarcoma cell lines(MG63).In this investigational study,MG63 cells were utilized.Microarray experiments,quantitative polymerase chain reaction(qPCR),immunofluorescent staining,extracellular acidification rate(ECAR),oxygen consumption rate(OCR),glucose consumption,lactate production,and ATP levels,proliferation assay,5-Ethynyl-2′-deoxyuridine(EDU)staining,and Western blot were performed.In MG63 cells,HYSA lowered cell proliferation and metastasis rates,suppressed EDU cell number,and enhanced caspase-3/9 activity levels.HYSA reduced the Warburg effect and induced ferroptosis(FPT)in MG63 cells.Inhibiting ferroptosis diminished HYSA’s anti-cancer activities in MG63 cells.The stimulation of the HIF-1α/SLC7A11 pathway decreased HYSA’s anti-cancer activities in MG63 cells.HIF-1αis one target spot for HYSA in a model of osteosarcoma cancer(OC).HYSA altered HIF-1α’s thermophoretic activity;following binding with HYSA,HIF-1α’s melting point increased from~55°C to~60°C.HYSA significantly enhanced the thermal stability of exogenous WT HIF-1αwhile not affecting Mut HIF-1α,suggesting that ARG-311,GLY-312,GLN-347,and GLN-387 may be involved in the interaction between HIF-1αand HYSA.Conclusively,our study revealed that HYSA induced FPT and reduced the Warburg effect of OC through mitochondrial damage by HIF-1α/HK2/SLC7A11 pathway.HYSA is a possible therapeutic option for OC or other cancers.

Effect of different drying methods on the taste and volatile compounds,sensory characteristics of Takifugu obscurus

Pufferfish is prone to deterioration due to abundant nutrients and high moisture content.Drying technology can extend the shelf life and enhance the flavor quality of aquatic products.The study investigated the effect of hot air drying(HAD),microwave vacuum drying(MVD)and hot air assisted radio frequency drying(HARFD)on the taste and volatile profiles of Takifugu obscurus.Different drying methods had significant influence on the color,rehydration,5’-nucleotides,free amino acids and volatile components(P<0.05).The results showed that HAD and HARFD could promote the flavor of T.obscurus by producing higher equivalent umami concentration(EUC)values,which were about two times of MVD group,and more pronounced pleasant odor according to sensory analysis.HAD is more appropriate for industrial application than HARFD and MVD considering the economic benefits.This study could provide a reference for the industrial application of drying T.obscurus.

A Dual Detection Method for Siemens Inverter Motor Modbus RTU Attack

Since the Modbus RTU wired communication protocol of Siemens variable frequency motors is unstable and lacks a protection mechanism, there is a risk of user information leakage. Aiming at the problems of insufficient flexibility of traditional defense methods and poor defense effects, The present work proposed a new dual detection method based on MODBUS RTU, which combines the dual monitoring mechanism of “Address Resolution Protocol (ARP) request detection” and “ARP response detection”. In order to improve detection efficiency, two real-time updated linear tables are introduced, which can effectively deal with the three ARP spoofing methods of updating the ARP buffer. Based on the analysis of the hidden dangers of the Modbus RTU wired communication protocol, a wired connection between the S7-1200 PLC and the variable frequency motor was established, and a real experimental platform was constructed to demonstrate the attack. The intensity of ARP attacks has gradually increased over time. Through comparative experiments with traditional defense methods, it is proved that the algorithm enhances the protocol mechanism in principle, and is more flexible and reliable than traditional methods.

Research on DoS Attack Detection Method of Modbus TCP in OpenPLC

With the development of new information technologies such as cloud computing, Internet of Things, and mobile Internet of Things, Industry 4.0, Smart Manufacturing and Made in China 2025 have been proposed as the main content of the development of the next industrial revolution. In order to realize these projects with the common characteristics of intelligence, service, and green, a new manufacturing model, digital twin, is proposed, which combines the digital twin with industrial systems, that is, the industrial control virtualization system. However, due to the frequent occurrence of industrial control system security incidents in recent years, the industrial control virtualization system is vulnerable to attacks. The industrial control system is huge and cumbersome. Once attacked, it will cause consequences that affect the whole body. In response to this problem, this article carried out a research on DoS attack detection methods for Modbus TCP in OpenPLC, using OpenPLC as a tool for industrial control system virtualization, building a digital twin system with Raspberry Pi, and launching DoS attacks on the system, combined with Snort Intrusion detection is carried out, and the experimental results show that the built digital twin system can detect DoS attacks in OpenPLC.

大规模能量收集物联网基于信息年龄的自适应接入

信息年龄是衡量通信系统传输时效性的重要性能指标,其优化对于实时检测监控和精准决策控制至关重要.尽管如此,针对多源多宿大规模物联网,现有研究缺乏考虑能量收集动态性、互干扰时空差异性以及接入参数时变性对信息年龄的综合影响.鉴于此,本文首次面向此类物联网提出一种允许发送节点在能量充足时根据对应实时信息年龄采用不同发送概率的自适应接入协议,并且联合运用空间点过程、马尔可夫(Markov)过程以及不动点理论推导了信息年龄空间分布和系统平均信息年龄,最后提出一种低复杂度获取次优自适应接入参数的方法.仿真结果验证了理论分析的准确性,并表明了所提自适应策略的信息年龄性能明显优于已有策略.

Research on Dance Evaluation Technology Based on Human Posture Recognition

In view of the increase in the number of people participating in dance rating assessments,this paper proposes a dance assessment technology based on human body posture recognition.This technique adopts the human target detection of the dance video,extracts bone key points,and then uses the video data set col-lected by professional dancers to conduct PoseC3D model training,enabling the model to classify the basic movements of the dance;then,the dynamic time nor-malization algorithm is used to evaluate the classified movements.The experimen-tal results show that this technology can accurately identify the basic movements of various dances and accurately give the evaluation score of the corresponding movements,thus reducing the work intensity of the assessment staff.

Flexible electrostatic hydrogels from marine organism for nitric oxide-enhanced photodynamic therapy against multidrug-resistant bacterial infection

The abuse of antibiotics in treating microbial infections has led to the emergence and prevalence of drugresistant bacteria.Thus,the development of novel antibacterial materials is attracting increasing attention.Here,a series of flexible electrostatic hydrogels with excellent antibacterial ability were constructed using a mixture of nitric oxide(NO)-releasing nitrated chitosan(CSNO)and mesotetra(4-carboxyphenyl)porphine(TCPP)with salmon sperm DNA(ssDNA)solution.When cultured with gram-negative bacteria under solar simulator irradiation,TCPP-CSNO_(m)ssDNA_(n) hydrogels released reactive oxygen species(ROS)and NO to produce peroxynitrite ions(ONOO^(−)).ONOO−is efficient at killing bacteria,thereby improving the antimicrobial ability of photodynamic therapy against gram-negative bacteria.The hydrogels exhibited powerful antibacterial activity in vivo when used to treat skin infections caused by drugresistant bacteria,making them a promising candidate for clinical applications.A string of antibacterial hydrogels that release ROS and NO synergistically can bring new possibilities for effectively killing drug-resistant bacteria and be of great value in anti-infection wound dressings and other applications.

Regioselective Synthesis of 2,6-Dimethyl-3,5-bis[（3-aryl-5- trifluoromethyl）-isoxazol-4-carbonyl]-pyridine Derivatives

Ethyl 4,4,4-trifluoro-3-oxo-butyrate reacted with 2,6-dimethyl-3,5-diacetyl-pyridine 1 in the presence of NaOC2H5 at 0 ℃ to give 2,6-dimethyl-3,5-bis（4,4,4-trifluoro-1,3-oxo-butyl）-pyridine （2a） in good yield. Cyclization reaction of 2a and aryl imidoyl chlorides 5a--5i, obtained from chlorination of aryl oximes with N-chlorosuccimide, afforded 2,6-dimethyl-3,5-bis[（3-aryl-5-trifluoromethyl）-isoxazol-4-carbonyl]-pyridine derivatives 6a=6i.

Application of acidic protease in the pickling to simplify the pelt bating process

Traditionally,universally used pelt bating technologies rely on the application of trypsin,neutral and alkaline microbial proteases but suffer from complicated operation,limited bating efficiency and unsatisfac-tory leather performance.Therefore,devising a new pelt bating approach to achieve high bating efficiency and excellent leather performance has always been wished for by the leather industry.To pursue this goal,years of persistent research work enabled us to develop a novel approach for pelt bating by means of acidic proteases in pickling process.Initially,basic enzymatic characteristics and bating effectiveness of several typical acidic pro-teases in pelt pickling medium were investigated;then,the bating effectiveness through the quantitative charac-terization of protease activity of the optimal acidic protease was compared with that of the conventional bating enzyme.The results indicated that all of the selected acidic proteases had good salt-tolerance and exhibited optimum activity at pH 3.0–4.0.The novel pickling-bating method based on microbial origin acidic protease L80A led to an outstanding performance on pelt bating at the dosage of 150 U/mL of collagenolytic activity.The bating effectiveness of acidic protease L80A was comparable to and even better than that of trypsin BEM due to its mod-erate proteolytic ability.Moreover,the deep and even penetration of acidic protease in the pelt permitted it to produce soft,organoleptically stable and overall better quality crust leather than that of the conventional trypsin bating method.Additionally,pelt bating was performed along with the pickling process without extra inactiva-tion and washing operation,making the bating operation more efficient,economical,and environment friendly.Results had made us to conclude that this cutting-edge acidic proteases based pickling-bating method could be the first step/way forward to replace the decades-old traditional pelt bating technology.

Automatic Generation of Graduation Thesis Comments Based on Multilevel Analysis

In the evaluation of graduation theses,teachers’evaluation criteria for graduation theses are inconsistent,subjective and not completely reasonable and fair.This paper proposes using the BERT model to analyze the existing graduation papers in colleges and universities and make quantitatively evaluate students’graduation projects according to the given relevant parameters.The purpose of this method is to use standards to make comprehensive,systematic and accurate evaluations and avoid the phenomenon of high repetition and similarity caused by a large number of teachers’comments.This can not only effectively improve the efficiency of graduation design evaluation but also improve the fairness of evaluation.In this paper,changing the review work of the graduation thesis from pure manual operation to machine review combined with manual operation can not only reduce manpower consumption but also make the review work more objective and fair,making it more objective on the basis of traditional subjective review.