Effect of a negative DC bias on a capacitively coupled Ar plasma operated at different radiofrequency voltages and gas pressures

The effect of a negative DC bias,|V_(dc)|,on the electrical parameters and discharge mode is investigated experimentally in a radiofrequency(RF)capacitively coupled Ar plasma operated at different RF voltage amplitudes and gas pressures.The electron density is measured using a hairpin probe and the spatio-temporal distribution of the electron-impact excitation rate is determined by phase-resolved optical emission spectroscopy.The electrical parameters are obtained based on the waveforms of the electrode voltage and plasma current measured by a voltage probe and a current probe.It was found that at a low|V_(dc)|,i.e.inα-mode,the electron density and RF current decline with increasing|V_(dc)|;meanwhile,the plasma impedance becomes more capacitive due to a widened sheath.Therefore,RF power deposition is suppressed.When|V_(dc)|exceeds a certain value,the plasma changes toα–γhybrid mode(or the discharge becomes dominated by theγ-mode),manifesting a drastically growing electron density and a moderately increasing RF current.Meanwhile,the plasma impedance becomes more resistive,so RF power deposition is enhanced with|V_(dc)|.We also found that the electrical parameters show similar dependence on|V_(dc)|at different RF voltages,andα–γmode transition occurs at a lower|V_(dc)|at a higher RF voltage.By increasing the pressure,plasma impedance becomes more resistive,so RF power deposition and electron density are enhanced.In particular,theα–γmode transition tends to occur at a lower|V_(dc)|with increase in pressure.

Ultrathin metal-organic framework nanosheets (Cu-TCPP)-based isothermal nucleic acid amplification for food allergen detection

The rapid and accurate detection of peanuts and soybeans allergen is important to the food safety. In this study, Cu-TCPP nanosheet, a kind of ultra-thin metal-organic framework(MOF)was synthesized and applied in loop-mediated isothermal amplification(named Cu-TCPP@LAMP), which can inhibit the non-specific amplification by absorbing and precise temperature releasing of single primer. As thus, Cu-TCPP@LAMP can achieve high sensitivity and specific amplification of the target gene. As a result, peanut and soybean allergens genes contained in food were successfully detected with a favorable detection sensitivity(5 ng/μL for peanuts and 10 ng/μL for soybeans)and reliable repeatability(The coefficient of variation was 3.38% for peanuts and 3.33% for soybeans). Moreover, the established method was utilized for detection of several commercial products, and had a high consistency with the standard method. Apart from food allergens, this novel assay can be widely used in other areas, such as pathogen detection, tumor nucleic acid detection and so on.

Paradigm of Numerical Simulation of SpatialWind Field for Disaster Prevention of Transmission Tower Lines

Numerical simulation of the spatial wind field plays a very important role in the study of wind-induced response law of transmission tower structures.A reasonable construction of a numerical simulation method of the wind field is conducive to the study of wind-induced response law under the action of an actual wind field.Currently,many research studies rely on simulating spatial wind fields as Gaussian wind,often overlooking the basic non-Gaussian characteristics.This paper aims to provide a comprehensive overview of the historical development and current state of spatial wind field simulations,along with a detailed introduction to standard simulation methods.Furthermore,it delves into the composition and unique characteristics of spatial winds.The process of fluctuating wind simulation based on the linear filter AR method is improved by introducing spatial correlation and non-Gaussian distribution characteristics.The numerical simulation method of the wind field is verified by taking the actual transmission tower as a calculation case.The results show that the method summarized in this paper has a broader application range and can effectively simulate the actual spatial wind field under various conditions,which provides a valuable data basis for the subsequent research on the wind-induced response of transmission tower lines.

Study on Flow Field Simulation at Transmission Towers in Loess Hilly Regions Based on Circular Boundary Constraints

When using high-voltage transmission lines for energy transmission in loess hilly regions,local extreme wind fields such as turbulence and high-speed cyclones occur from time to time,which can cause many kinds of mechanical and electrical failures,seriously affecting the reliable and stable energy transmission of the power grid.The existing research focuses on the wind field simulation of ideal micro-terrain and actual terrain with mostly single microterrain characteristics.Model boundary constraints and the influence of constrained boundaries are the main problems that need to be solved to accurately model and simulate complex flow fields.In this paper,a flow field simulation method based on circular boundary constraints is carried out.During the study,the influence of the model boundary and the selection conditions of themodeling range are systematically analyzed.It is more suitable to make sure that the air domain is 4 times higher than the height of the hill undulations,in addition to ensuring that there should be a minimum of 400 m between the study region and the boundary.Then,an actual terrain model of a power grid line in Shanxi is established,through the method proposed in this paper,the wind speed at the location of the transmission tower line under different wind directions is analyzed,and it is found that when the incidence direction is 45 degrees north by east the wind speed is the highest.The findings demonstrate that the circular boundary model has the advantage of more easily adjusting the wind incidence direction,in addition to theoretically reducing the errors caused by traditional models in boundary processing.It can accurately obtain the distribution characteristics of the flow field affected by the terrain,and quickly screen out the extreme working conditions that are most harmful to the transmission lines in the actual transmission line for energy transmission in complex loess hilly regions.

Optimization of Supercritical Extraction Process of Laoshan Black Tea by Response Surface Methodology

[Objectives]Laoshan black tea was subjected to supercritical CO_(2) extraction. [Methods]The extraction conditions of Laoshan black tea were studied by an orthogonal experiment and optimized by response surface methodology. [Results] The optimum extraction conditions of black tea extract by supercritical CO_(2) extraction were as follows: extraction pressure 23.53 MPa, extraction time 1.73 h, and extraction temperature 49.75 ℃, with which the extract yield could reach 5.15% theoretically. [Conclusions] Based on the traditional extraction process, a supercritical extraction method optimized by response surface methodology and a unique extraction process were formed, which enriches the extraction processes and methods of natural raw materials.

Experimental Measurement of the Generalized Stokes Parameters of a Radially Polarized Random Electromagnetic Beam

Utilizing the Young’s double slits and Mach-Zehnder interferometer, we proposed an experimental method to measure the generalized Stokes parameters of a radially polarized random electromagnetic beam. After the partially coherent beam propagating through the Young’s double slits, the interference fringe is obtained by the help of a Mach-Zehnder interferometer consisting of apertures, quarter-wave plates and polarizers. The electric cross-spectral density matrix is detected by the coherence degree of interference fringe and the density of each single slit. The generalized Stokes parameters can be obtained from the electric cross-spectral density matrix. This experiment measures the generalized Stokes parameters of the random electromagnetic beam successfully. The results show that the spectral degree of coherence for copolarized cases (xx and yy) is similar with that for cross-polaried cases (xy and yx) for the radially polarized random electromagnetic beam. This method will help us determine the change of the polarization and coherence of the light in propagation by detecting the change of the generalized Stokes parameters.

水稻典型品种日本晴和IR24根系微生物组的解析

植物的各项生命活动与其根系微生物组密不可分,且根系微生物组的组成易受到植物生长环境和基因型的影响。为进一步探究中国北方地区种植的不同品种水稻根系微生物组的差异及其相互作用机制,本研究以种植于北京昌平和上庄农场的水稻典型品种日本晴(Nipponbare)和IR24为研究对象,基于16S rRNA基因扩增子测序技术获得根系微生物组序列,利用多样性分析、组成型分析、机器学习的随机森林和网络分析等方法,对旺盛生长期的两种不同品种的水稻根系微生物组进行详细比较。研究发现,种植地点和水稻基因型显著影响了水稻根系微生物组的群落结构,不同基因型导致了根系微生物组在物种分类组成上以及细菌间相互关系的差异,而且根系微生物组能作为生物标记跨地点区分宿主的基因型。本研究结果为深入理解我国北方种植的水稻根系微生物组的组成规律以及从根系微生物与植物互作的角度对品种进行改良提供了数据和理论基础。

微生物组数据分析方法与应用

高通量测序技术的发展衍生出一系列微生物组(microbiome)研究技术,如扩增子、宏基因组、宏转录组等,快速推动了微生物组领域的发展。微生物组数据分析涉及的基础知识、软件和数据库较多,对于同领域研究者开展学习和选择合适的分析方法具有一定困难。本文系统概述了微生物组数据分析的基本思想和基础知识,详细总结比较了扩增子和宏基因组分析中的常用软件和数据库,并对高通量数据下游分析中常用的几种方法,包括统计和可视化、网络分析、进化分析、机器学习和关联分析等,从可用性、软件选择以及应用等几个方面进行了概述。本文拟通过对当前微生物组主流分析方法的整理和总结,为同领域研究者更方便、灵活的开展数据分析,快速选择研究分析工具,高效挖掘数据背后的生物学意义提供参考,进一步推动微生物组研究在生物学领域的发展。

Pilot Domain NOMA for Grant-Free Massive Random Access in Massive MIMO Marine Communication System

We propose a pilot domain non-orthogonal multiple access(NOMA)for uplink massive devices grant-free random access scenarios in massive multiple-input multiple-output(MIMO)maritime communication systems.These scenarios are characterized by numerous devices with sporadic access behavior,and therefore only a subset of them are active.Due to massive potential devices in the network,it is infeasible to assign a unique orthogonal pilot to each device in advance.In such scenarios,pilot decontamination is a crucial problem.In this paper,the devices are randomly assigned non-orthogonal pilots which are constructed by a linear combination of some orthogonal pilots.We show that a bipartite graph can conveniently describe the interference cancellation(IC)processes of pilot decontamination.High spectrum efficiency(SE)and low outage probability can be obtained by selecting the numbers of orthogonal pilots according to the given probability distribution.Numerical evaluatioDs show that the proposed pilot domain NOMA decreases the outage probability from 20%to 2 e-12 at the SE of 4 bits/s/Hz for a single device,compared to the conventional method of slotted ALOHA with 1024 antennas at the BS,or increases the spectrum efficiency from 1.2 bits/s/Hz to 4 bit/s/Hz at the outage probability of2 e-12 in contrast with the Welch bound equality(WBE)non-orthogonal pilots.

Experimental investigation of the electromagnetic effect and improvement of the plasma radial uniformity in a large-area,very-high frequency capacitive argon discharge

We performed an experimental investigation on the electromagnetic effect and the plasma radial uniformity in a larger-area, cylindrical capacitively coupled plasma reactor. By utilizing a floating hairpin probe, dependences of the plasma radial density on the driving frequency and the radio-frequency power over a wide pressure range of 5-40 Pa were presented. At a relatively low frequency(LF, e.g. 27 MHz), an evident peak generally appears near the electrode edge for all pressures investigated here due to the edge field effect, while at a very high frequency(VHF, e.g.60 or 100 MHz), the plasma density shows a sharp peak at the discharge center at lower pressures, indicating a strong standing wave effect. As the RF power increases, the center-peak structure of plasma density becomes more evident. With increasing the pressure, the standing wave effect is gradually overwhelmed by the ‘stop band’ effect, resulting in a transition in the plasma density profile from a central peak to an edge peak. To improve the plasma radial uniformity, a LF source is introduced into the VHF plasma by balancing the standing wave effect with the edge effect. A much better plasma uniformity can be obtained if one chooses appropriate LF powers, pressures and other corresponding discharge parameters.

Magnetic probe diagnostics of nonlinear standing waves and bulk ohmic electron power absorption in capacitive discharges

It is recognized that standing wave effects appearing in large-area,very-high-frequency capacitively coupled plasma(CCP)reactors cause center-high plasma non-uniformity.Using a high-frequency magnetic probe,we present a direct experimental diagnostic of the nonlinear standing waves and bulk ohmic electron power absorption dynamics in low pressure CCP discharges for different driving frequencies of 13.56,30,and 60 MHz.The design,principle,calibration,and validation of the probe are described in detail.Spatial structures of the harmonics of the magnetic field,determined by the magnetic probe,were used to calculate the distributions of the harmonic current and the corresponding ohmic electron power deposition,providing insights into the behavior of nonlinear harmonics.At a low driving frequency,i.e.13.56 MHz,no remarkable nonlinear standing waves were identified and the bulk ohmic electron power absorption was observed to be negligible.The harmonic magnetic field/current was found to increase dramatically with the driving frequency,due to decreased sheath reactance and more remarkable nonlinear standing waves at a higher driving frequency,leading to the enhancements of the ohmic heating and the plasma density in the bulk,specifically at the electrode center.At a high driving frequency,i.e.60 MHz,the high-order harmonic current density and the corresponding ohmic electron power absorption exhibited a similar node structure,with the main peak on axis,and one or more minor peaks between the electrode center and the edge,contributing to the center-high profile of the plasma density.

The rice histone methylation regulates hub species of the root microbiota

Plants have a close relationship with their root microbiota,which comprises a complex microbial network.Histone methylation is an important epigenetic modification influencing multiple plant traits;however,little is known about the role of plant histone methylation in the assembly and network structure of the root microbiota.In this study,we established that the rice(Oryza sativa)histone methylation regulates the structure and composition of the root microbiota,especially the hub species in the microbial network.DJjmj703(defective in histone H3K4 demethylation)and ZH11-sdg714(defective in H3K9 methylation)showed significant different root microbiota compared with the corresponding wild types at the phylum and family levels,with a consistent increase in the abundance of Betaproteobacteria and a decrease in the Firmicutes.In the root microbial network,35 of 44 hub species in the top 10 modules in the tested field were regulated by at least one histone methylation-related gene.These observations establish that the rice histone methylation plays a pivotal role in regulating the assembly of the root microbiota,providing insights into the links between plant epigenetic regulation and root microbiota.

Vision-based adaptive variable rate spraying approach for unmanned aerial vehicles

The rapid developments of unmanned aerial vehicles(UAV)and vision sensor are contributing a great reformation in precision agriculture.Farmers can fly their UAV spraying pesticides around their crop fields while staying at their remote control room or any place that is separated from their farm land.However,there is a common phenomenon in rice planting management stage that some empty areas are randomly located in farmland.Therefore,a critical problem is that the waste of pesticides that occurs when spraying pesticides over rice fields with empty areas by using the common UAV,because it is difficult to control the flow accuracy based on the empty areas changing.To tackle this problem,a novel vision-based spraying system was proposed that can identify empty areas automatically while spraying a precise amount of pesticides on the target regions.By this approach,the image was preprocessed with the Lucy-Richardson algorithm,then the target area was split from the background with k-means and the feature parameters were extracted,finally the feature parameters were filtered out with a positive contribution which would serve as the input parameters of the support vector machine(SVM)to identify the target area.Also a fuzzy control model was analyzed and exerted to compensate the nonlinearity and hysteresis of the variable rate spraying system.Experimental results proved that the approach was applicable to reducing the amount of pesticides during UAV spraying,which can provide a reference for precision agriculture aviation in the future.

China is initiating the Aquatic 10-100-1,000 Genomics Program

On June 17 of 2016,a group of scientists from Chinese Academy of Fishery Sciences（CAFS）and Shenzhen BGI Fisheries Sci＆Tech Co.Ltd.（BGI Fisheries）met together in Shenzhen,China to launch a novel project,the China Aquatic 10-100-1,000 Genomics Program,to conduct molecular breeding of-10 aquaculture species and sequence genomes of-100 and transcriptomes of-1,000 aquatic species.

Ultrasensitive detection of IgE levels based on magnetic nanocapturer linked immunosensor assay for early diagnosis of cancer

Rapid and accurate detection of immunoglobulin E(Ig E) in serum and reduction of serum dosage are of great significance for clinical detection. Herein, we described a rapid magnetic separation of Ig E from patient serum based on Fe3 O4@Si O2-NTA@026 sdab as the capture probe and multiple horseradish peroxidase(HRP)-labeled antibodies linked gold nanoparticles(Au NPs) as chemiluminescence(CL) signal amplifier for ultrasensitive detection of total Ig E. Results showed that the limit of detection of our immunosensor system in serum samples was 0.03 k U/L, which is lowest in comparison with current methods, and far lower than that of Immuno CAP for Ig E detection(0.1 k U/L). Furthermore, our immunosensor possessed satisfied repeatability and accuracy, as well as good stability. In comparison with the Immuno CAP for the quantitative detection of Ig E, highly consistent results were achieved in 20 serum samples. Specially, this method was also successfully utilized for assessing the Ig E traces in breast cancer patients,which provides a new idea for the diagnosis of early cancer. Therefore, we believe that such versatile immunosensor will offer an alternative method for the on-site monitoring and determination of various Ig E-related diseases.