Sex pheromones are considered to play critical roles in partner communication of most parasitic Hymenoptera.However,the identification of sex pheromone components remains limited to a few families of parasitoid wasps....Sex pheromones are considered to play critical roles in partner communication of most parasitic Hymenoptera.However,the identification of sex pheromone components remains limited to a few families of parasitoid wasps.In this study,we functionally characterized a candidate sex pheromone component in Microplitis mediator(Hymenoptera:Braconidae),a solitary parasitoid of Noctuidae insects.We found that the body surface extract from female wasps could significantly stimulate courtship behavior of males.Gas chromatography-electroantennographic detection(GC-EAD)analysis revealed that a candidate semiochemical from extract triggered significant electrophysiological response of antennae of males.By performing gas chromatography-mass spectrometer(GC-MS)measurement,GC-EAD active compound was identified as n-octyl acrylate,a candidate sex pheromone component in female M.mediator.In electroantennogram(EAG)tests,antennae of male wasps showed significantly higher electrophysiological responses to n-octyl acrylate than those of females.Y-tube olfactometer assays indicated that male wasps significantly chose n-octyl acrylate compared with the control.Furthermore,male wasps showed a remarkable preference for n-octyl acrylate in a simulated field condition behavioral trial;simultaneously,n-octyl acrylate standard could also trigger significant courtship behavior in males.We propose that n-octyl acrylate,as a candidate vital sex pheromone component,could be utilized to design behavioral regulators of M.mediator to implement the protection and utilization of natural enemies.展开更多
基金supported by the National Natural Science Foundation of China(32150410366,31972338,and 32372639)China Agriculture Research System(CARS-02-26)the National Key Research and Development Program of China(2021YFD1400700).
文摘Sex pheromones are considered to play critical roles in partner communication of most parasitic Hymenoptera.However,the identification of sex pheromone components remains limited to a few families of parasitoid wasps.In this study,we functionally characterized a candidate sex pheromone component in Microplitis mediator(Hymenoptera:Braconidae),a solitary parasitoid of Noctuidae insects.We found that the body surface extract from female wasps could significantly stimulate courtship behavior of males.Gas chromatography-electroantennographic detection(GC-EAD)analysis revealed that a candidate semiochemical from extract triggered significant electrophysiological response of antennae of males.By performing gas chromatography-mass spectrometer(GC-MS)measurement,GC-EAD active compound was identified as n-octyl acrylate,a candidate sex pheromone component in female M.mediator.In electroantennogram(EAG)tests,antennae of male wasps showed significantly higher electrophysiological responses to n-octyl acrylate than those of females.Y-tube olfactometer assays indicated that male wasps significantly chose n-octyl acrylate compared with the control.Furthermore,male wasps showed a remarkable preference for n-octyl acrylate in a simulated field condition behavioral trial;simultaneously,n-octyl acrylate standard could also trigger significant courtship behavior in males.We propose that n-octyl acrylate,as a candidate vital sex pheromone component,could be utilized to design behavioral regulators of M.mediator to implement the protection and utilization of natural enemies.
