To improve the reliability and reduce energy consumption,a conceptual design of a freeze valve is proposed for the thorium-based molten salt reactor(TMSR)concept.Fins were utilized in this new design to enhance heat t...To improve the reliability and reduce energy consumption,a conceptual design of a freeze valve is proposed for the thorium-based molten salt reactor(TMSR)concept.Fins were utilized in this new design to enhance heat transfer and realize passive shut-off function,which could not be realized by the previous design.An experimental apparatus using the fluoride salt FLiNaK was constructed to conduct a series of preliminary solidification and melting experiments.In addition,the enthalpy-porosity method of ANSYS■Fluent solver was applied to simulate the solidification process of the salt at a specified operating temperature.Temperature distributions of the fluoride salt,solidification/melting time,and frozen plug effect were analyzed under natural convection heat transfer in an open space.The calculated salt temperatures exhibited good agreement with the experimental values.The results indicated that the range of effective operating temperature is 530-600℃ for the finned freeze valve.In this study,the ideal set operating temperature of the finned freeze valve was chosen as 560℃ to achieve competent performance.Moreover,560℃ is additionally the highest set operating temperature for maintaining excellent cooling performance and sustaining deep-frozen condition of the salt plug.At this set operating temperature,the simulation data indicated that the molten salt in the flat part of the finned freeze valve will completely solidify at 10.5 min.The percentage of solid salt in the flat and lower transitional parts of the valve reaches 29.60% in 30.0 min.Furthermore,the surface temperature of the proposed freeze valve is 11.10% lower compared with that of the TMSR freeze valve at a cooling gas supply of 173 m^3/h.Therefore,the new freeze valve was proven to be capable of reducing the energy consumption and realizing the passive shut-off function.展开更多
The transcription factor early growth response protein 3 (EGR3) is involved in schizophrenia. We developed a putative rat model of schizophrenia by transfecting lentiviral particles carrying the Egr3 gene into bilat...The transcription factor early growth response protein 3 (EGR3) is involved in schizophrenia. We developed a putative rat model of schizophrenia by transfecting lentiviral particles carrying the Egr3 gene into bilateral hippocampal dentate gyrus. We assessed spatial working memory using the Morris water maze test, and neuronal metabolite levels in bilateral hippocampus and thalamus were determined by 3.0 T proton magnetic resonance spectroscopy. Choline content was significantly greater in the hippocampus after transfection, while N-acetylaspartate and the ratio of N-acetylaspartate to creatine/phosphocreatine in the thalamus were lower than in controls. This study is the first to report evaluation of brain metabolites using 3,0 T proton magnetic resonance spectroscopy in rats transfected with Egr3, and reveals metabolic abnormalities in the hippocampus and thalamus in this putative model of schizophrenia.展开更多
The high-temperature molten salt pump is the core equipment in a molten salt reactor that drives the flow of the molten salt coolant.Rotor stability is key to the continuous and reliable operation of the molten salt p...The high-temperature molten salt pump is the core equipment in a molten salt reactor that drives the flow of the molten salt coolant.Rotor stability is key to the continuous and reliable operation of the molten salt pump,and the liquid seal at the wear ring can affect the dynamic characteristics of the rotor system.When the molten salt pump is operated in the hightemperature molten salt medium,thermal deformation of the submerged parts inevitably occurs,changing clearance between the stator and rotor,affecting the leakage and dynamic characteristics of the seal.In this study,the seal leakage,seal dynamic characteristics,and rotor system dynamic characteristics are simulated and analyzed using finite element simulation software based on two cases of considering the effect of seal thermal deformation effect or not.The results show a significant difference in the leakage characteristics and dynamic characteristics of the seal obtained by considering the effect of seal thermal deformation and neglecting the effect of thermal deformation.The leakage flow rate decreases,and the first-order critical speed of the seal-bearing-rotor system decrease after considering the seal’s thermal deformation.展开更多
Molten salt pump is applied to pump high-temperature molten salt as the primary coolant of a molten salt reactor.The pump,generally a vertical rotor system,suffers from radial force generated by the liquid seal compon...Molten salt pump is applied to pump high-temperature molten salt as the primary coolant of a molten salt reactor.The pump,generally a vertical rotor system,suffers from radial force generated by the liquid seal component,and the rotordynamic characteristics of the pump are affected considerably.In this paper,the rotordynamic coefficients of the tooth-on-stator liquid seal in molten salt pump are studied.The flow in the seal region is simulated using computational fluid dynamics technique.Parameters of the inlet loss and the pre-swirl at the inlet region of the seal are calculated.The coefficients of resistance and the wall parameters are obtained from the simulation results by data fitting method.The rotordynamic coefficients are analyzed based on the bulk-flow model of liquid seal.The rotordynamic characteristics,with and without the liquid seal,of the objective molten salt pump are inspected.The first critical speed of the rotor is found to increase.Harmonic analysis shows that the pump,being sensitive to unbalance force though,can operate safely under its design specifications.展开更多
We consider a two-way relay network where the Amplify-and-Forward (AF) protocol is adopted by all relays in this paper.The network consists of two multi-antenna source nodes and multiple distributed single-antenna rel...We consider a two-way relay network where the Amplify-and-Forward (AF) protocol is adopted by all relays in this paper.The network consists of two multi-antenna source nodes and multiple distributed single-antenna relays.Two opportunistic relaying schemes are proposed to efficiently utilize the antennas of the source nodes and the relay nodes.In the first scheme,the best relay is selected out by a max-min-max criterion before transmitting.After that,at each source,only the antenna with the largest channel gain between itself and the best relay is activated to transmit and receive signals with full power.In the second scheme,assisted by the best relay which is selected by the typical max-min criterion,both source nodes use all their antennas to exchange data,and match filter beamforming techniques are employed at both source nodes.Further analyses show that all schemes can achieve the full diversity order,and the conclusions are not only mathematically demonstrated but numerically illustrated.System performance comparisons are carried out by numerical methods in terms of rate sum and outage probability,respectively.The beamforming assisted scheme can be found to be superior to the antenna selection scheme when accurate Channel State Information (CSI) is available at the transmitters.Otherwise,the latter is very suitable.展开更多
Based on cloud-probe data and airborne Ka-band cloud radar data collected in Baoding on 5 August 2018,the microphysical structural characteristics of cumulus(Cu)cloud at the precipitation stage were investigated.The c...Based on cloud-probe data and airborne Ka-band cloud radar data collected in Baoding on 5 August 2018,the microphysical structural characteristics of cumulus(Cu)cloud at the precipitation stage were investigated.The cloud droplets in the Cu cloud were found to be significantly larger than those in stratiform(STF)cloud.In the Cu cloud,most cloud particles were between 7 and 10μm in diameter,while in the STF cloud the majority of cloud particles grew no larger than 2μm.The sensitivity of cloud properties to aerosols varied with height.The cloud droplet effective radius showed a negative relationship with the aerosol number concentration(Na)in the cloud planetary boundary layer(PBL)and upper layer above the PBL.However,the cloud droplet concentration(Nc)varied little with decreased Na in the high liquid water content region above 1500 m.High Na values of between 300 and 1853 cm-3 were found in the PBL,and the maximum Na was sampled near the surface in August in the Hebei region,which was lower than that in autumn and winter.High radar reflectivity corresponded to large FCDP(fast cloud droplet probe)particle concentrations and small aerosol particle concentrations,and vice versa for low radar reflectivity.Strong updrafts in the Cu cloud increased the peak radius and Nc,and broadened cloud droplet spectrum;lower air temperature was favorable for particle condensational growth and produced larger droplets.展开更多
observation data obtained in a mesoscale convective system are compared to Weather Research and Forecasting(WRF)model simulations using four microphysics schemes(Morrison,WSM6,P3,SBM)with different complexities.The ma...observation data obtained in a mesoscale convective system are compared to Weather Research and Forecasting(WRF)model simulations using four microphysics schemes(Morrison,WSM6,P3,SBM)with different complexities.The main purpose of this paper is to assess the performance of the microphysics ensemble in terms of cloud microphysical properties.Results show that although the vertical distributions of liquid water content(LWC)and ice water content(IWC)simulated by the four members are quite different in the convective cloud region,they are relatively uniform in the stratiform cloud region.Overall,the results of the Morrison scheme are very similar to the ensemble average,and both of them are closer to the observations compared to the other schemes.Besides,the authors also note that all members still overpredict the LWC by a factor of 2–8 in some regions,resulting in large deviation between the observation and ensemble average.展开更多
Alzheimer's disease (AD) is a neurodegenerative disease characterized by progressive memory loss and cognitive dysfunction. Currently there is no effective treatment. Given the depth of current stem cell research,...Alzheimer's disease (AD) is a neurodegenerative disease characterized by progressive memory loss and cognitive dysfunction. Currently there is no effective treatment. Given the depth of current stem cell research, researchers have attempted to compensate for neuronal loss in the brains of patients with Alzheimer's disease using stem cell technology. This article will review the pathogenesis of AD, the latest advances in stem cell therapy, and the challenges and prospects of its development.展开更多
Waldenstrom's macroglobulinemia/lymphoplasmacytic lymphoma (WM/LPL) is a low-grade B-cell non- Hodgkin's lymphoma with an indolent clinical course. Higher-grade non-Hodgkin lymphoma (NHL) and therapy- related my...Waldenstrom's macroglobulinemia/lymphoplasmacytic lymphoma (WM/LPL) is a low-grade B-cell non- Hodgkin's lymphoma with an indolent clinical course. Higher-grade non-Hodgkin lymphoma (NHL) and therapy- related myelodysplasia/acute leukemia (t-MDS/AML) have been reported in patients with WM/LPL in previous studies. However, only two cases with WM/LPL were reported to develop to Hodgkin lymphoma (HL). Here, we report the first case of WM/LPL who developed classical HL simultaneously 3 years after initial nucleoside analog-based chemotherapy.展开更多
Objective:To construct a recombinant lentiviral vector that co-express ZsGreen and BDNF and establish an Adipose derived stem cell (ADSC) line with stable BDNF expression down- regulation.Methods: BDNF were designed a...Objective:To construct a recombinant lentiviral vector that co-express ZsGreen and BDNF and establish an Adipose derived stem cell (ADSC) line with stable BDNF expression down- regulation.Methods: BDNF were designed and inserted into the lentiviral vector p HBLV-CMVIE-Zs Green-Puro. After identification by DNA sequencing, the lentiviral vectors carrying BDNF were packaged in 293 cells. The transfection efficiency was observed under fluorescence microscope;the lentiviral particles were collected to infect mouse ADSCs, and BDNF level were assessed using real-time PCR and Western blotting.Results: DNA sequencing demonstrated successful construction of the BDNF lentivirus vectors. Real- time PCR and Western blotting showed a high level BDNF mRNA and protein.Conclusion: We have successfully established an ADSC model with stable BDNF expression which establish the basis for the potential application of ADSCs-BDNF in the treatment of AD.展开更多
Plant root-nodule symbiosis(RNS)with mutualistic nitrogen-fixing bacteria is restricted to a single clade of angiosperms,the Nitrogen-Fixing Nodulation Clade(NFNC),and is best understood in the legume family.Nodulatin...Plant root-nodule symbiosis(RNS)with mutualistic nitrogen-fixing bacteria is restricted to a single clade of angiosperms,the Nitrogen-Fixing Nodulation Clade(NFNC),and is best understood in the legume family.Nodulating species share many commonalities,explained either by divergence from a common ancestor over 100 million years ago or by convergence following independent origins over that same time period.Regardless,comparative analyses of diverse nodulation syndromes can provide insights into constraints on nodulation—what must be acquired or cannot be lost for a functional symbiosis—and the latitude for Plant Communications Genomic landscape of nodulation variation in the symbiosis.However,much remains to be learned about nodulation,especially outside of legumes.Here,we employed a large-scale phylogenomic analysis across 88 species,complemented by 151 RNA-seq libraries,to elucidate the evolution of RNS.Our phylogenomic analyses further emphasize the uniqueness of the transcription factor NIN as a master regulator of nodulation and identify key muta-tions that affect its function across the NFNC.Comparative transcriptomic assessment revealed nodule-specific upregulated genes across diverse nodulating plants,while also identifying nodule-specific and nitrogen-response genes.Approximately 70%of symbiosis-related genes are highly conserved in the four representative species,whereas defense-related and host-range restriction genes tend to be lineage specific.Our study also identified over 900000 conserved non-coding elements(CNEs),over 300000 of which are unique to sampled NFNC species.NFNC-specific CNEs are enriched with the active H3K9ac mark and are correlated with accessible chromatin regions,thus representing a pool of candidate regula-tory elements for genes involved in RNS.Collectively,our results provide novel insights into the evolution of nodulation and lay a foundation for engineering of RNS traits in agriculturally important crops.展开更多
Positive effects of fermented foods consumption on humans have stimulated lots of research attention.The aim of this study was to determine the effect of Lactobacillus paracasei M-8R on the physicochemical and antioxi...Positive effects of fermented foods consumption on humans have stimulated lots of research attention.The aim of this study was to determine the effect of Lactobacillus paracasei M-8R on the physicochemical and antioxidant properties of mead during its fermentation.The mixed fermentation mead with Lactobacillus paracasei M-8R had an alcohol content of 17%,higher total polyphenols(107.14 mg GAE/L)and total flavonoids(74.53 mg RE/L)and the highest antioxidant capacity.The control sample(fermented only by yeast)was successive:11.2%,94 mg GAE/L and 68.74 mg RE/L.The most abundant volatile flavor compounds(80 species)were detected in the mead fermented by Saccharomyces Only.Combined with sensory evaluation,the mixed fermentation of Saccharomyces cerevisiae G10 and Lactobacillus paracasei M-8R is considered as the more promising fermentation method for mead production.展开更多
基金supported by the National Natural Science Foundation of China(No.91326201)the Strategic Pilot Technology Chinese Academy of Sciences(No.XDA0201002)
文摘To improve the reliability and reduce energy consumption,a conceptual design of a freeze valve is proposed for the thorium-based molten salt reactor(TMSR)concept.Fins were utilized in this new design to enhance heat transfer and realize passive shut-off function,which could not be realized by the previous design.An experimental apparatus using the fluoride salt FLiNaK was constructed to conduct a series of preliminary solidification and melting experiments.In addition,the enthalpy-porosity method of ANSYS■Fluent solver was applied to simulate the solidification process of the salt at a specified operating temperature.Temperature distributions of the fluoride salt,solidification/melting time,and frozen plug effect were analyzed under natural convection heat transfer in an open space.The calculated salt temperatures exhibited good agreement with the experimental values.The results indicated that the range of effective operating temperature is 530-600℃ for the finned freeze valve.In this study,the ideal set operating temperature of the finned freeze valve was chosen as 560℃ to achieve competent performance.Moreover,560℃ is additionally the highest set operating temperature for maintaining excellent cooling performance and sustaining deep-frozen condition of the salt plug.At this set operating temperature,the simulation data indicated that the molten salt in the flat part of the finned freeze valve will completely solidify at 10.5 min.The percentage of solid salt in the flat and lower transitional parts of the valve reaches 29.60% in 30.0 min.Furthermore,the surface temperature of the proposed freeze valve is 11.10% lower compared with that of the TMSR freeze valve at a cooling gas supply of 173 m^3/h.Therefore,the new freeze valve was proven to be capable of reducing the energy consumption and realizing the passive shut-off function.
文摘The transcription factor early growth response protein 3 (EGR3) is involved in schizophrenia. We developed a putative rat model of schizophrenia by transfecting lentiviral particles carrying the Egr3 gene into bilateral hippocampal dentate gyrus. We assessed spatial working memory using the Morris water maze test, and neuronal metabolite levels in bilateral hippocampus and thalamus were determined by 3.0 T proton magnetic resonance spectroscopy. Choline content was significantly greater in the hippocampus after transfection, while N-acetylaspartate and the ratio of N-acetylaspartate to creatine/phosphocreatine in the thalamus were lower than in controls. This study is the first to report evaluation of brain metabolites using 3,0 T proton magnetic resonance spectroscopy in rats transfected with Egr3, and reveals metabolic abnormalities in the hippocampus and thalamus in this putative model of schizophrenia.
基金the Strategic Pilot Technology Chinese Academy of Sciences(No.XDA02010500).
文摘The high-temperature molten salt pump is the core equipment in a molten salt reactor that drives the flow of the molten salt coolant.Rotor stability is key to the continuous and reliable operation of the molten salt pump,and the liquid seal at the wear ring can affect the dynamic characteristics of the rotor system.When the molten salt pump is operated in the hightemperature molten salt medium,thermal deformation of the submerged parts inevitably occurs,changing clearance between the stator and rotor,affecting the leakage and dynamic characteristics of the seal.In this study,the seal leakage,seal dynamic characteristics,and rotor system dynamic characteristics are simulated and analyzed using finite element simulation software based on two cases of considering the effect of seal thermal deformation effect or not.The results show a significant difference in the leakage characteristics and dynamic characteristics of the seal obtained by considering the effect of seal thermal deformation and neglecting the effect of thermal deformation.The leakage flow rate decreases,and the first-order critical speed of the seal-bearing-rotor system decrease after considering the seal’s thermal deformation.
基金funded by the National Natural Science Foundation of China(Nos.51576125 and 51406114)
文摘Molten salt pump is applied to pump high-temperature molten salt as the primary coolant of a molten salt reactor.The pump,generally a vertical rotor system,suffers from radial force generated by the liquid seal component,and the rotordynamic characteristics of the pump are affected considerably.In this paper,the rotordynamic coefficients of the tooth-on-stator liquid seal in molten salt pump are studied.The flow in the seal region is simulated using computational fluid dynamics technique.Parameters of the inlet loss and the pre-swirl at the inlet region of the seal are calculated.The coefficients of resistance and the wall parameters are obtained from the simulation results by data fitting method.The rotordynamic coefficients are analyzed based on the bulk-flow model of liquid seal.The rotordynamic characteristics,with and without the liquid seal,of the objective molten salt pump are inspected.The first critical speed of the rotor is found to increase.Harmonic analysis shows that the pump,being sensitive to unbalance force though,can operate safely under its design specifications.
基金supported by the National Natural Science Foundation of China under Grant No.60902092
文摘We consider a two-way relay network where the Amplify-and-Forward (AF) protocol is adopted by all relays in this paper.The network consists of two multi-antenna source nodes and multiple distributed single-antenna relays.Two opportunistic relaying schemes are proposed to efficiently utilize the antennas of the source nodes and the relay nodes.In the first scheme,the best relay is selected out by a max-min-max criterion before transmitting.After that,at each source,only the antenna with the largest channel gain between itself and the best relay is activated to transmit and receive signals with full power.In the second scheme,assisted by the best relay which is selected by the typical max-min criterion,both source nodes use all their antennas to exchange data,and match filter beamforming techniques are employed at both source nodes.Further analyses show that all schemes can achieve the full diversity order,and the conclusions are not only mathematically demonstrated but numerically illustrated.System performance comparisons are carried out by numerical methods in terms of rate sum and outage probability,respectively.The beamforming assisted scheme can be found to be superior to the antenna selection scheme when accurate Channel State Information (CSI) is available at the transmitters.Otherwise,the latter is very suitable.
基金funded by the National Key Research and Devel-opment Program of China[grant number 2017YFC1501405]the National Natural Science Foundation of China[grant numbers 41975180,41705119,and 41575131]the National Center of Meteorology,Abu Dhabi,AE(UAE Research Program for Rain Enhancement Science)。
文摘Based on cloud-probe data and airborne Ka-band cloud radar data collected in Baoding on 5 August 2018,the microphysical structural characteristics of cumulus(Cu)cloud at the precipitation stage were investigated.The cloud droplets in the Cu cloud were found to be significantly larger than those in stratiform(STF)cloud.In the Cu cloud,most cloud particles were between 7 and 10μm in diameter,while in the STF cloud the majority of cloud particles grew no larger than 2μm.The sensitivity of cloud properties to aerosols varied with height.The cloud droplet effective radius showed a negative relationship with the aerosol number concentration(Na)in the cloud planetary boundary layer(PBL)and upper layer above the PBL.However,the cloud droplet concentration(Nc)varied little with decreased Na in the high liquid water content region above 1500 m.High Na values of between 300 and 1853 cm-3 were found in the PBL,and the maximum Na was sampled near the surface in August in the Hebei region,which was lower than that in autumn and winter.High radar reflectivity corresponded to large FCDP(fast cloud droplet probe)particle concentrations and small aerosol particle concentrations,and vice versa for low radar reflectivity.Strong updrafts in the Cu cloud increased the peak radius and Nc,and broadened cloud droplet spectrum;lower air temperature was favorable for particle condensational growth and produced larger droplets.
基金supported by the National Key R&D Program of Chinagrant number 2018YFC1507900the Demonstration Project of Artificial Precipitation Enhancement and Hail Suppression Operation Technology at the Eastern Side of the Taihang Mountains grant number hbrywcsy-2017-2sponsored by the National Natural Science Foundation of China grant numbers 41530427 and 41875172。
文摘observation data obtained in a mesoscale convective system are compared to Weather Research and Forecasting(WRF)model simulations using four microphysics schemes(Morrison,WSM6,P3,SBM)with different complexities.The main purpose of this paper is to assess the performance of the microphysics ensemble in terms of cloud microphysical properties.Results show that although the vertical distributions of liquid water content(LWC)and ice water content(IWC)simulated by the four members are quite different in the convective cloud region,they are relatively uniform in the stratiform cloud region.Overall,the results of the Morrison scheme are very similar to the ensemble average,and both of them are closer to the observations compared to the other schemes.Besides,the authors also note that all members still overpredict the LWC by a factor of 2–8 in some regions,resulting in large deviation between the observation and ensemble average.
基金National Natural Science Foundation of China (81660224,31760310)
文摘Alzheimer's disease (AD) is a neurodegenerative disease characterized by progressive memory loss and cognitive dysfunction. Currently there is no effective treatment. Given the depth of current stem cell research, researchers have attempted to compensate for neuronal loss in the brains of patients with Alzheimer's disease using stem cell technology. This article will review the pathogenesis of AD, the latest advances in stem cell therapy, and the challenges and prospects of its development.
文摘Waldenstrom's macroglobulinemia/lymphoplasmacytic lymphoma (WM/LPL) is a low-grade B-cell non- Hodgkin's lymphoma with an indolent clinical course. Higher-grade non-Hodgkin lymphoma (NHL) and therapy- related myelodysplasia/acute leukemia (t-MDS/AML) have been reported in patients with WM/LPL in previous studies. However, only two cases with WM/LPL were reported to develop to Hodgkin lymphoma (HL). Here, we report the first case of WM/LPL who developed classical HL simultaneously 3 years after initial nucleoside analog-based chemotherapy.
基金The National Natural Science Foundation of China.Project No:(81660224,81100246)Hainan Provincial Key Research and Development Project.Project No:(ZDYF2017120,ZDYF2016110)+2 种基金National College’s Students’Innovation and Entrepreneurship Training Program Project.Project No:(201711810010,HYCX2016010,HYCX2018032)Hainan Provincial Health and Family Planning Industry Research Project.Project No:(18A200115)Haikou People’s Hospital Project.Project No:(2018-SNP-01-003).
文摘Objective:To construct a recombinant lentiviral vector that co-express ZsGreen and BDNF and establish an Adipose derived stem cell (ADSC) line with stable BDNF expression down- regulation.Methods: BDNF were designed and inserted into the lentiviral vector p HBLV-CMVIE-Zs Green-Puro. After identification by DNA sequencing, the lentiviral vectors carrying BDNF were packaged in 293 cells. The transfection efficiency was observed under fluorescence microscope;the lentiviral particles were collected to infect mouse ADSCs, and BDNF level were assessed using real-time PCR and Western blotting.Results: DNA sequencing demonstrated successful construction of the BDNF lentivirus vectors. Real- time PCR and Western blotting showed a high level BDNF mRNA and protein.Conclusion: We have successfully established an ADSC model with stable BDNF expression which establish the basis for the potential application of ADSCs-BDNF in the treatment of AD.
基金supported by the National Natural Science Foundation of China (32022006)the Program for Guangdong"ZhuJiang"Innovation Teams (2019ZT08N628)+6 种基金the Agricultural Science and Technology Innovation Program (ASTIP) (CAAS-XTCX2016001)the special funds for science technology innovation and industrial development of Shenzhen Dapeng New District (PT202101-01)supported by the National Natural Science Foundation of China (32070250)the Natural Science Foundation of Guangdong Province (2020A1515011030)the open research project of"Cross-Cooperative Team"of the Germplasm Bank of Wild Species,Kunming Institute of Botany,Chinese Academy of Sciencessupported by the Natural Science Foundation of Guangdong Province (2022A1515110240)support was provided by IRD and the Agence Nationale de la Recherche (Project SESAM,2010 BLAN 170801).
文摘Plant root-nodule symbiosis(RNS)with mutualistic nitrogen-fixing bacteria is restricted to a single clade of angiosperms,the Nitrogen-Fixing Nodulation Clade(NFNC),and is best understood in the legume family.Nodulating species share many commonalities,explained either by divergence from a common ancestor over 100 million years ago or by convergence following independent origins over that same time period.Regardless,comparative analyses of diverse nodulation syndromes can provide insights into constraints on nodulation—what must be acquired or cannot be lost for a functional symbiosis—and the latitude for Plant Communications Genomic landscape of nodulation variation in the symbiosis.However,much remains to be learned about nodulation,especially outside of legumes.Here,we employed a large-scale phylogenomic analysis across 88 species,complemented by 151 RNA-seq libraries,to elucidate the evolution of RNS.Our phylogenomic analyses further emphasize the uniqueness of the transcription factor NIN as a master regulator of nodulation and identify key muta-tions that affect its function across the NFNC.Comparative transcriptomic assessment revealed nodule-specific upregulated genes across diverse nodulating plants,while also identifying nodule-specific and nitrogen-response genes.Approximately 70%of symbiosis-related genes are highly conserved in the four representative species,whereas defense-related and host-range restriction genes tend to be lineage specific.Our study also identified over 900000 conserved non-coding elements(CNEs),over 300000 of which are unique to sampled NFNC species.NFNC-specific CNEs are enriched with the active H3K9ac mark and are correlated with accessible chromatin regions,thus representing a pool of candidate regula-tory elements for genes involved in RNS.Collectively,our results provide novel insights into the evolution of nodulation and lay a foundation for engineering of RNS traits in agriculturally important crops.
基金This work was supported by the Plan of Science and Technology Department of Jilin Province of China[20210202057NC].
文摘Positive effects of fermented foods consumption on humans have stimulated lots of research attention.The aim of this study was to determine the effect of Lactobacillus paracasei M-8R on the physicochemical and antioxidant properties of mead during its fermentation.The mixed fermentation mead with Lactobacillus paracasei M-8R had an alcohol content of 17%,higher total polyphenols(107.14 mg GAE/L)and total flavonoids(74.53 mg RE/L)and the highest antioxidant capacity.The control sample(fermented only by yeast)was successive:11.2%,94 mg GAE/L and 68.74 mg RE/L.The most abundant volatile flavor compounds(80 species)were detected in the mead fermented by Saccharomyces Only.Combined with sensory evaluation,the mixed fermentation of Saccharomyces cerevisiae G10 and Lactobacillus paracasei M-8R is considered as the more promising fermentation method for mead production.