Research on Reform and Innovation of Curriculum System of Construction Equipment Specialty based on BIM application

BIM technology entered China at the beginning of this century and has played a prominent role in many industries,Firstly,it is applied in design enterprises,and then extends to construction enterprises,with emphasis on construction engineering.As the main force in the development of the construction industry,construction enterprises pay more and more attention to the learning and application of BIM in enterprises.BIM technology will bring more possibilities to construction equipment.With the development of The Times,the original teaching technology has been unable to meet and achieve students'future career needs and goals.This paper follows closely the steps of teaching reform,With the application of BIM technology as the entry point.The reform and innovation of BIM are deeply integrated into the professional core courses,curriculum design and graduation design,etc,in order to keep up with the industry demand and employment guidance,improve the social competitiveness of student’s employment.

Radiomics models to predict bone marrow metastasis of neuroblastoma using CT

Background:Bone marrow is the leading site for metastasis from neuroblastoma and affects the prognosis of patients with neuroblastoma.However,the accurate diagnosis of bone marrow metastasis is limited by the high spatial and temporal heterogeneity of neuroblastoma.Radiomics analysis has been applied in various cancers to build accurate diagnostic models but has not yet been applied to bone marrow metastasis of neuroblastoma.Methods:We retrospectively collected information from 187 patients pathologically diagnosed with neuroblastoma and divided them into training and validation sets in a ratio of 7:3.A total of 2632 radiomics features were retrieved from venous and arterial phases of contrastenhanced computed tomography(CT),and nine machine learning approaches were used to build radiomics models,including multilayer perceptron(MLP),extreme gradient boosting,and random forest.We also constructed radiomics‐clinical models that combined radiomics features with clinical predictors such as age,gender,ascites,and lymph gland metastasis.The performance of the models was evaluated with receiver operating characteristics(ROC)curves,calibration curves,and risk decile plots.Results:The MLP radiomics model yielded an area under the ROC curve(AUC)of 0.97(95%confidence interval[CI]:0.95–0.99)on the training set and 0.90(95%CI:0.82–0.95)on the validation set.The radiomics‐clinical model using an MLP yielded an AUC of 0.93(95%CI:0.89–0.96)on the training set and 0.91(95%CI:0.85–0.97)on the validation set.Conclusions:MLP‐based radiomics and radiomics‐clinical models can precisely predict bone marrow metastasis in patients with neuroblastoma.

纳秒激光调控CFRP复合材料表面润湿性及其对胶接性能的影响

采用纳秒激光对碳纤维增强树脂基(CFRP)复合材料进行表面预处理,调控其表面成分、粗糙度和表面润湿性,然后采用SEM、接触角测量仪、光学轮廓仪、XPS等表征CFRP复合材料的表面微观形貌、接触角、粗糙度和化学成分,并通过拉伸剪切实验评价和分析激光表面处理对CFRP复合材料胶接强度的影响规律和机制。结果表明:优化激光表面处理参数,可以去除CFRP复合材料表面的环氧树脂胶,调控其表面成分、粗糙度和表面润湿性;与未处理的CFRP复合材料相比,激光表面处理后的CFRP复合材料表面化学成分改变,表面粗糙度有所增加,润湿性提高,胶接强度也增大;与未处理CFRP复合材料相比,激光离焦量分别为5 mm、10 mm和15 mm时,处理后的CFRP复合材料胶接强度分别提高了129.41%、112.13%和105.88%;激光表面处理CFRP复合材料的表面润湿性和表面粗糙度均高于机械处理CFRP复合材料,但激光处理导致的热损伤对胶接强度提高有负面影响。

Fe_3O_4@C nanoparticles as high-performance Fenton-like catalyst for dye decoloration

Water pollution has become serious environmental problem nowadays. Advanced oxidation processes(AOP) have been widely applied in water treatment.However, traditional Fenton reaction based on Fe2﹢-H2O2 system has obvious drawbacks, which limit its applications In this study, magnetic Fe3O4core-C shell nanoparticles(Fe3O4@C NPs) were prepared for the decoloration of methylene blue(MB) via the co-precipitation followed by the hydrothermal dehydrogenation of glucose. Fe3O4@C NPs showed high catalytic activity of the decoloration of MB through the decomposition of H2O2 in Fenton-like reactions. Fe3O4@C NPs had much higher activity than bare Fe3O4 cores, suggesting the coating of carbon enhanced the catalytic activity. The performance of Fe3O4@C NPs was better at lower pH and higher temperature, but was significantly inhibited in the presence of radical scavenger tertiary butanol. Fe3O4@C NPs could be magnetic separated and regenerated, and maintained with very good catalytic activity. The implication for the applications of Fe3O4@C NP-catalyzed Fenton-like reactions in water treatment was discussed.

PEGylation of Double-Walled Carbon Nanotubes for Increasing Their Solubility in Water

Polyethylene glycol(PEG)functionalized double-walled carbon nanotubes(DWNTs)have been synthesized by a[2+1]cycloaddition reaction and characterized by transmission electron microscopy,atomic force microscopy,Raman spectroscopy,thermal gravimetric analysis,and UV-visible spectroscopy.Functionalization affords a large increase in the aqueous solubility of DWNTs.The saturated concentrations of DWNTs functionalized with diazido-terminated PEG800(with a molecular weight of 800)and azido-terminated PEG750 monomethylether(with a molecular weight of 750)are very similar-0.36 and 0.37 mg/mL(DWNTs equivalent concentration),respectively.

Interaction of multi-walled carbon nanotubes and zinc ions enhances cytotoxicity of zinc ions

More and more nanomaterials enter the environment along with their production, application and deposal. They may alter the biological effect of pollutants already existing in the real environment by different interactions. Therefore efforts should also be paid to investigate the combined toxicity of nanomaterials and pollutants. Herein, we studied the combined toxicity of oxi- dized multi-walled carbon nanotubes （O-MWCNTs） and zinc ions on ceils. It is found that cytotoxicity of the combined O-MWCNTs and zinc ions elevates significantly, compared with O-MWCNTs or zinc ions alone. This result comes from the assays of cell morphology, cell viability and proliferation, cell membrane integrity, mitochondrial membrane potential and cell apoptosis. Mechanism studies indicate that O-MWCNTs absorb zinc ions and form slight aggregation. These enhance remark- ably the cellular uptake of O-MWCNTs, and thus induce the death of cells by bringing in more zinc ions into cells. Our study indicates that the existence of nanomaterials could change the bioconsequence of other pollutants and emphasizes the im- portance of the combined toxicity research in the presence of nanomaterials.

Direct Imaging of Titania Nanotubes Located in Mouse Neural Stem Cell Nuclei

Titania nanotubes(TiO2-NTs)are a potential drug vehicle for use in nanomedicine.To this end,a preliminary study of the interaction of a model cell with TiO2-NTs has been carried out.TiO2-NTs were first conjugated with a fl uorescent label,fl uorescein isothiocyanate(FITC).FITC-conjugated titania nanotubes(FITC-TiO2-NTs)internalized in mouse neural stem cells(NSCs,line C17.2)can be directly imaged by confocal microscopy.The confocal imaging showed that FITC-TiO2-NTs readily entered into the cells.After co-incubation with cells for 24 h,FITC-TiO2-NTs localized around the cell nucleus without crossing the karyotheca.More interestingly,the nanotubes passed through the karyotheca entering the cell nucleus after co-incubation for 48 h.Atomic force microscopy(AFM)and transmission electron microscopy(TEM)were also employed in tracking the nanotubes in the cell.These results will be of benefit in future studies of TiO2-NTs for use as a drug vehicle,particularly for DNA-targeting drugs.

Machine‐learning radiomics to predict bone marrow metastasis of neuroblastoma using magnetic resonance imaging

Background: Neuroblastoma is one common pediatric malignancy notorious forhigh temporal and spatial heterogeneities. More than half of its patients developdistant metastases involving vascularized organs, especially the bone marrow. It isthus necessary to have an economical, noninvasive method without muchradiation for follow‐ups. Radiomics has been used in many cancers to assistaccurate diagnosis but not yet in bone marrow metastasis in neuroblastoma.Methods: A total of 182 patients with neuroblastoma were retrospectivelycollected and randomly divided into the training and validation sets. Fivehundredand seventy‐two radiomics features were extracted from magneticresonance imaging, among which 41 significant ones were selected via T‐testfor model development. We attempted 13 machine‐learning algorithms andeventually chose three best‐performed models. The integrative performanceevaluations are based on the area under the curves (AUCs), calibration curves,risk deciles plots, and other indexes.Results: Extreme gradient boosting, random forest (RF), and adaptiveboosting were the top three to predict bone marrow metastases in neuroblastoma while RF was the most accurate one. Its AUC was 0.90(0.86–0.93), F1 score was 0.82, sensitivity was 0.76, and negative predictivevalue was 0.79 in the training set. The values were 0.82 (0.71–0.93), 0.80,0.75, and 0.92 in the validation set, respectively.Conclusions: Radiomics models are likely to contribute more to metastaticdiagnoses and the formulation of personalized healthcare strategies in clinics.It has great potential of being a revolutionary method to replace traditionalinterventions in the future.

Silica nanoparticle with a single His-tag for addressable functionalization, reversible assembly, and recycling

For many biomedical and catalytic applications, such as encapsulation of proteins/enzymes in nanopartides （NPs）, it is preferable to have well-dispersed small NPs that are stable in solution and behave quasi-homogeneously. However, conventional liquid phase methods for small-NP synthesis and functionalization usually face great difficulties in separation/purification and recycling. In addition, controlling the orientation of proteins inside NPs is also a crucial issue to maximize the activity of the encapsulated proteins. Herein, we report a solid phase method to solve these problems. Using His-tagged proteins as cores, well-dispersed core-sheU silica NPs are facilely synthesized and functionalized in a column. The core His-tagged proteins are kept bound on the surface of the resIn beads in the column during the entire process, making the separation/purification of NPs and their precursors during the multiple-step process as simple as a few-minutes procedure of draining and washing the column. Each obtained silica NP has an adjustable eccentric core-shell structure with only one His-tag sticking out of the particle. This single His-tag on the surface of each NP not only makes it easy for addressable and stoichiometric functionalization of the NP but also provides an easy way to reversibly assemble NPs into dimers or be oriented on the surface of large particles. Notably, this solid phase approach also provides a versatile means to control the orientation of proteins inside NPs, and the His-tag makes it easy to recycle those well-dispersed small NPs.

The conformation alteration of mouse hepatic histones after reacting with nicotine in vitro

UV differential spectroscopy, fluorescence spectroscopy and circular dichroism (CD) spectroscopy assays have been applied to studying the conformation alteration of mouse hepatic histones H1 and H3 after reacting with nicotine in vitro. The results indicate that their conformation changes from regular form to random form with the increasing reaction dose of nicotine. The adduction of nicotine or its metabolites with histones H1 and H3 accounts for the conformation alteration. Nicotine may affect the structure, function and expression of genes of chromosome by changing the conformation of histones.