基于FLEXPART模型的复杂地形大气扩散能力研究——以山西省孝义市和古县为例

基于2012—2021年地面气象观测资料,采用无人机观测气温廓线,计算分别位于开阔盆地和狭长山谷地形的山西省孝义市和古县大气混合层厚度、大气自净能力指数、逆温层厚度;利用FLEXPART模型模拟两地不同时期(清洁期、重污染期)大气中颗粒物传输路径及高度.结果表明:孝义市和古县大气自净能力指数分别为13.4和6.2,大气自净能力等级分别为二级和四级,春季大气自净能力最强,冬季最弱.冬季古县逆温强度大于孝义市,不利于大气污染物的扩散.清洁时期,两地污染物均沿西北方向传输,孝义市大气中粒子传输高度较高,传输距离更远;重污染期,两地空气中粒子传输高度均偏低,传输距离受地形影响,易造成当地大气污染.因此,开阔盆地相较于狭长山谷地区,大气污染物更易清除;开阔盆地大气污染物传输高度高于狭长山谷地区,且传输距离也更远.

The DNA damage repair complex MoMMS21-MoSMC5 is required for infection-related development and pathogenicity of Magnaporthe oryzae

The conserved DNA damage repair complex,MMS21-SMC5/6(Methyl methane sulfonate 21-Structural maintenance of chromosomes 5/6),has been extensively studied in yeast,animals,and plants.However,its role in phytopathogenic fungi,particularly in the highly destructive rice blast fungus Magnaporthe oryzae,remains unknown.In this study,we functionally characterized the homologues of this complex,MoMMS21 and MoSMC5,in M.oryzae.We first demonstrated the importance of DNA damage repair in M.oryzae by showing that the DNA damage inducer phleomycin inhibited vegetative growth,infection-related development and pathogenicity in this fungus.Additionally,we discovered that MoMMS21 and MoSMC5 interacted in the nuclei,suggesting that they also function as a complex in M.oryzae.Gene deletion experiments revealed that both MoMMS21 and MoSMC5 are required for infection-related development and pathogenicity in M.oryzae,while only MoMMS21 deletion affected growth and sensitivity to phleomycin,indicating its specific involvement in DNA damage repair.Overall,our results provide insights into the roles of MoMMS21 and MoSMC5 in M.oryzae,highlighting their functions beyond DNA damage repair.

Complement factor Ⅰ knockdown inhibits colon cancer development by affecting Wnt/β-catenin/c-Myc signaling pathway and glycolysis

BACKGROUND Colon cancer(CC)occurrence and progression are considerably influenced by the tumor microenvironment.However,the exact underlying regulatory mechanisms remain unclear.AIM To investigate immune infiltration-related differentially expressed genes(DEGs)in CC and specifically explored the role and potential molecular mechanisms of complement factor I(CFI).METHODS Immune infiltration-associated DEGs were screened for CC using bioinformatics.Quantitative reverse transcription polymerase chain reaction was used to examine hub DEGs expression in the CC cell lines.Stable CFI-knockdown HT29 and HCT116 cell lines were constructed,and the diverse roles of CFI in vitro were assessed using CCK-8,5-ethynyl-2’-deoxyuridine,wound healing,and transwell assays.Hematoxylin and eosin staining and immunohistochemistry staining were employed to evaluate the influence of CFI on the tumorigenesis of CC xenograft models constructed using BALB/c male nude mice.Key proteins associated with glycolysis and the Wnt pathway were measured using western blotting.RESULTS Six key immune infiltration-related DEGs were screened,among which the expression of CFI,complement factor B,lymphoid enhancer binding factor 1,and SRY-related high-mobility-group box 4 was upregulated,whereas that of fatty acid-binding protein 1,and bone morphogenic protein-2 was downregulated.Furthermore,CFI could be used as a diagnostic biomarker for CC.Functionally,CFI silencing inhibited CC cell proliferation,migration,invasion,and tumor growth.Mechanistically,CFI knockdown downregulated the expression of key glycolysis-related proteins(glucose transporter type 1,hexokinase 2,lactate dehydrogenase A,and pyruvate kinase M2)and the Wnt pathway-related proteins(β-catenin and c-Myc).Further investigation indicated that CFI knockdown inhibited glycolysis in CC by blocking the Wnt/β-catenin/c-Myc pathway.CONCLUSION The findings of the present study demonstrate that CFI plays a crucial role in CC development by influencing glycolysis and the Wnt/β-catenin/c-Myc pathway,indicating that it could serve as a promising target for therapeutic intervention in CC.

柚皮苷通过HIF-1α/VEGF信号促进H型血管抗骨质疏松的研究

目的观察柚皮苷对去卵巢骨质疏松大鼠骨组织HIF-1α/VEGF信号通路和H型血管形成的影响,探讨柚皮苷防治骨质疏松症的作用机制。方法建立去卵巢骨质疏松大鼠模型(随机将36只大鼠分为去卵巢组、假手术组和柚皮苷治疗组)。柚皮苷治疗3个月后,检测血清和骨髓上清骨代谢指标和HIF-1α/VEGF信号通路变化,检测骨密度(bone mineral density,BMD)、骨微结构及H型血管变化。结果柚皮苷治疗组大鼠血清中成骨代谢指标[骨特异性碱性磷酸酶(BALP)、骨钙素(BGP)、I型原胶原分子N端前肽(PINP)]升高;而骨吸收标志物血清C端交联肽(CTX)降低;HIF-1α在血清和骨髓上清中表达均升高;VEGF在骨髓上清中表达升高,而血清中无明显改变;BMD和Micro-CT结果显示柚皮苷治疗组大鼠骨密度和骨量增加;同时股骨近端H型血管表达升高。结论柚皮苷可以调节骨代谢,改善骨质疏松大鼠骨密度,发挥抗骨质疏松作用,其作用机制可能是通过调节HIF-1α/VEGF信号通路、促进H型血管形成来实现的。

单侧上尿路梗阻解除前后肾皮质厚度与肾小球滤过率的关系

目的探讨单侧上尿路梗阻解除前后肾皮质厚度(RCT)与肾小球滤过率(GFR)的关系。方法选取2015年7月-2016年6月该院泌尿外科收治的63例上泌尿道梗阻患者为研究对象。其中,男34例,女29例,年龄22~73(52.21±11.56)岁;手术方式:输尿管镜碎石+取石术24例,一期经皮肾造瘘+二期经皮肾镜取石术(PCNL)21例,一期PCNL 18例。采用腹部增强CT、单光子发射计算机断层(SPECT)扫描分别测量RCT和GFR,根据术前患肾GFR情况分为3组:A组(中度肾损害,15.0 ml/min≤GFR<30.0 ml/min)、B组(重度肾损害,7.5 ml/min≤GFR<15.0 ml/min)、C组(极重度肾损害,GFR<7.5 ml/min),比较3组的手术前后的GFR变化。采用简单线性相关分析A和B组患者术前RCT与各指标的相关性。结果 9例患者因肾积水严重未测量RCT,患者的术前RCT>10 mm 20例,5~10 mm 23例,<5 mm 11例,平均值为(10.75±4.91)mm。A组术前RCT明显高于B组,差异有统计学意义(P<0.05)。3组患者术后1和9个月的GFR均明显高于同组术前,差异均有统计学意义(P<0.05)。A组术后1和9个月的GFR均明显高于另外两组,B组术后9个月的GFR明显高于C组,差异均有统计学意义(P<0.05),但术后1个月B组和C组的GFR差异无统计学意义(P>0.05)。B组术后1个月的GFR恢复值明显低于另外两组,差异有统计学意义(P<0.05),3组术后9个月的GFR恢复值比较差异无统计学意义(P>0.05)。术前RCT与术前GFR(r=0.613)、术后1个月GFR(r=0.697)及恢复值(r=0.552)、术后9个月GFR(r=0.589)及恢复值(r=0.488)均呈显著正相关,差异有统计学意义(P<0.05)。结论解除上泌尿道梗阻后患者的肾功能得到不同程度的恢复,即使是极重度肾功能损伤者,多数均能挽回患肾。术前RCT能有助于判断围手术期的肾功能,尤其是术后肾功能恢复情况。

Effects of Supplemental Feeding of Probiotics during Lactation on Rumen Microflora of Calves after Weaning

For the experiment, 8 newborn male Holstein calves were selected that had the same feeding environment, and were of similar ages. They were randomly divided into 2 groups, with 4 in each group. The treatments consisted of feeding active probiotics (Group P) and a normal fed control group (Group C). The growth performance and blood indices were measured;rumen fluid samples were collected after weaning, and 16SrDNA sequencing and LC-MS metabolome detection were performed. Compared with the control group, relative abundances of Deltaproteobacteria, Desulfovibrionales, Bacteroidales_ BS11_gut_group, Desulfovibrionaceae, Bacteroidales_S24-7_group, Acetobacteraceae, Ruminococcaceae_NK4A214_group, Asaia, [Ruminococcus] gauvreauii_group, Desulfovibrio, Kingella, Selenomonas, Lachnoclostridium in group P were significantly different (P < 0.05). In group P, the metabolite of 2-methylbenzoic acid and myo-inositol were significantly increased (P < 0.05). These results showed that compared with normally fed calves, the growth performance and blood indices of probiotic-fed calves were changed, but the differences were not significant. Probiotic-fed calves showed significant differences in rumen fluid and a small number of metabolites, which were mainly involved in the pathway of carbohydrate metabolism. It proves that the supplemental active probiotics had an effect on the rumen microflora.

一种边缘和过渡区域相结合的红外目标提取方法

传统的基于过渡区域提取的目标分割算法存在噪声敏感问题,从而会影响到过渡区域提取的准确性。与可见光图像相比,红外图像特别是红外光谱图像,受到探测器无法消除的热噪声影响,传统的目标提取算法准确率普遍降低。此外,虽然通过边缘能够精确定位目标,但是无法获取目标完整边缘。而过渡区域的灰度分布特点是可以解决基于边缘的目标提取难题。因此为了提高目标提取的抗噪性和准确性,提出了一种将过渡区域提取与边缘检测结合的自适应红外目标提取方法。首先利用像元空间邻域信息构造密度,以此有效降低噪声影响和获取图像边缘信息。然后基于像元密度信息最大分离目标边缘与背景,得到有效边缘和过渡区域,进而以此生长出目标。将边缘与过渡区域结合,可以很好地抑制噪声,多幅复杂场景实验评估了该方法的抗噪性能,结果显示,提出的方法在噪声的干扰下能较好的提取目标。

法尼酯衍生物X受体基因多态性与代谢性疾病的相关性

法尼酯X受体(farnesoid X receptor,FXR),是由配体激活、维持机体稳态的一种转录因子。作为一种多功能核受体,入核后与视黄醛衍生物受体(retinoid X receptor,RXR)形成异质二聚体,与反向重复序列-1(inverted repeat-1,IR1)结合调节靶基因表达。其靶基因涉及脂肪酸、胆汁酸、葡萄糖代谢,进而决定了FXR与胆汁淤积、糖尿病、高血压、脂质紊乱等代谢性疾病的密切联系。研究表明,多个FXR基因多态性位点与代谢性疾病的发生密切相关,如rs56163822与糖代谢紊乱相关,rs35724和rs10860603会影响身体质量指数(body mass index,BMI)。本文拟就近年来FXR参与代谢途径及其基因多态性与代谢性疾病的相关性作一综述,为代谢性疾病的发生几率评估及提前干预提供新思路。

糖脂代谢在新型冠状病毒肺炎普通型和重型患者中的特点分析及比较

目的·分析比较新型冠状病毒肺炎(coronavirus disease 2019,COVID-19)普通型和重型患者的糖脂代谢特点。方法·2020年2—3月,将武汉雷神山医院普通病房住院的36例COVID-19普通型患者和重症监护室(intensive care unit,ICU)的50例COVID-19重型患者设为普通型患者组和重型患者组,对他们的电子病历进行提取和分析。收集2组的人口学资料、临床资料、实验室检查结果、合并疾病和临床结局,并采用独立样本t检验、非参数检验、χ^(2)检验等比较2组之间的差异。从糖脂代谢角度初步分析COVID-19普通型和重型患者的特点及患者入住ICU的相关因素。结果·2组患者的性别、合并糖尿病或冠心病的人数间无统计学差异;重型患者平均年龄较普通型患者明显偏大(P<0.05);重型患者组患高血压的比例(52.0%)显著高于普通型患者(22.2%)(P<0.05);重型患者组淋巴细胞计数明显低于普通型患者组(P<0.05);2组患者间谷丙转氨酶、谷草转氨酶、血清肌酐、血尿酸值无统计学差异;重型患者组血清白蛋白、校正钙离子浓度、总胆固醇、三酰甘油、高密度脂蛋白和低密度脂蛋白均显著低于普通型患者组(均P<0.05);重型患者组的空腹血糖水平显著高于普通型患者组(P=0.001)。多因素Logistic回归发现空腹血糖、总胆固醇、高密度脂蛋白、低密度脂蛋白、血清白蛋白水平与COVID-19患者入住ICU存在一定的相关性。结论·COVID-19患者,尤其是重型患者,存在更加严重的糖脂代谢紊乱。空腹血糖、总胆固醇、高密度脂蛋白、低密度脂蛋白和血清白蛋白水平可能与患者入住ICU相关。

CYP2R1的遗传多态性及其个体化治疗应用

CYP2R1是细胞色素P450家族2亚家族的成员,作为维生素D的25-羟化酶,负责将维生素D羟基化为25-羟基维生素D(25-OH-D),影响活性激素1,25(OH)2D的生成。CYP2R1突变会引发维生素D依赖型佝偻病1B型,且CYP2R1的多态性与25-OH-D水平显著相关,尤其是rs10741657。CYP2R1基因多态性会影响其蛋白表达水平和酶活性,对其催化的代谢过程产生显著影响。CYP2R1通过调节基因功能,从而调节免疫反应,影响1型糖尿病的发病风险。其多态性与多种癌症的风险相关,包括乳腺癌,前列腺癌和结肠直肠癌。研究CYP2R1基因多态性有利于深入了解代谢途径的改变在相关疾病发生发展中的作用,加深对癌症、佝偻病、1型糖尿病等疾病的认识,促进从基因层面检测和评估相关疾病的发生风险。本文拟就近五年CYP2R1参与的代谢途径和基因多态性对其影响的研究进展作一综述。

不同番茄品种幼苗期耐盐性鉴定

为筛选番茄耐盐种质资源,给番茄抗盐栽培提供理论依据及参考,以20个番茄品种为试材,4叶1心时在3种不同NaCl浓度(40 mmol/L、80 mmol/L、120 mmol/L)的盐胁迫下进行幼苗期耐盐鉴定。盐胁迫处理10 d后,测定各个番茄品种的形态指标(株高、茎粗)及生理指标(丙二醛含量、质膜透性、可溶性蛋白质含量、脯氨酸含量、SOD活性、POD活性、CAT活性),以聚类分析为依据,比较20个番茄品种苗期的耐盐性。结果表明:不同番茄品种的过氧化氢酶活性、过氧化物酶活性这两项指标随盐溶液浓度的增加酶活性的变化差异也随之增大。120 mmol/L NaCl浓度胁迫下,耐盐性较强的番茄品种的脯氨酸含量明显低于耐盐性弱的品种,丙二醛含量明显高于耐盐性较弱的品种,可溶性蛋白质含量则无明显变化。低浓度(40 mmol/L)的盐溶液可促进番茄植株生长,但随盐浓度增加,明显表现出抑制番茄幼苗生长。20个番茄品种中,耐盐性强的3个品种为爱美特、凌派、宝迪,耐盐性弱的4个品种为卡西欧、紫罗兰、富康、粉桃。

Effect of High Concentrate Corn Stalk Particle Size on Physical State and Production Performance of Lactating Dairy Cows

In order to realize the application of corn stalk in cow feed, we designed experiments to explore the effect of a certain proportion of corn stalk on the performance of lactating dairy cows. 9 multiparous mid-lactating cows were allocated at random to three groups, each containing 3 intact cows. The trial consisted of three periods and three dietary treatments with a 3 × 3 Latin square design. The diets were normal concentrats plus dried corn stalk chopped to 5 - 8 cm long (N), high concentrates plus dried corn stalks chopped to a length of approximately 5 - 8 cm by a mower (H) while the milled corn stalks were passed through a pulviser with a 2 cm pore size (MH). Each cow was measured for dry matter intake (DMI), ruminal pH, rumen fermentation, selective feeding behavior and production performance. The results showed that MH led to a significantly higher intake of DM, neutral detergent fiber (NDF), forage NDF (FNDF), acid detergent fiber (ADF), crude protein (CP) and organic matter (OM) than N and H (P < 0.05). Cows fed H and MH showed similar selective feeding behavior, while those fed H showed various selectivity for the dietary component. MH resulted in a significantly higher milk production (P < 0.05), and tended to have a higher milk fat production than N (P = 0.055). There were no significant differences in the milk components (P = 0.424) and lactose (P = 0.113) between cows fed N and MH. The high-concentrates plus milled corn stalk diet can increase the milk yield under the premise of normal rumen pH in dairy cows, thereby generating higher economic benefits. And milled corn stalk can effectively inhibit the cow’s selective eating of low-quality roughage.

Effects of Supplementation with <i>Saccharomyces cerevisiae</i>and Its Fermentation Products on Production Performance and Its Mechanism in Perinatal Dairy Cows

The aim of this study was to investigate the effects of Saccharomyces cerevisiae and its fermentation products on performance, blood hormone levels and rumen floral composition in peripartum dairy cows. Sixty perinatal cows were selected and allocated to two groups according to parity and expected date of delivery. Each group was supplemented with Saccharomyces cerevisiae and its fermentation product 0 or 100 g. The results showed that Saccharomyces cerevisiae and its fermentation products could significantly increase the feed intake of peripartum dairy cows (P < 0.01), increase the lactose content after 21 days postpartum (P < 0.01), and tend to increase milk production (P = 0.052). There was no significant effect on other milk components, the apparent digestibility of nutrients. There was a tendency to increase milk production and reduce the number of somatic cells in milk;increase blood levels of glucagon (P < 0.01) and β-hydroxybutyrate (P = 0.01), reducing the insulin content (P = 0.02). Saccharomyces cerevisiae reduced the abundance of rumen microbes in peripartum dairy cows but had no effect on rumen microbial diversity. Compared with the control group, the supplemented group showed reductions in the abundance of genera Bacillus (P = 0.03), Butyrivibrio (P = 0.01), Denitrobacterium (P = 0.01), and Mogibacterium (P < 0.01), Porphyromonas (P = 0.05), Saccharofermentans (P < 0.01), Sphaerochaeta (P = 0.02), Streptococcus (P = 0.04) and other genera. There were significant increase in the content of Acidaminococcus (P = 0.03), Allisonella (P < 0.01), Bulleidia (P < 0.01), Corynebacterium (P = 0.01), Dialister (P < 0.01), Faecalibacterium (P = 0.02), Faekalitalea (P = 0.03), Fibrobacter (P = 0.04), Flavobacterium (P = 0.03), Kandleria (P < 0.01), Paraprevotella (P < 0.01), Pyramidobacter (P = 0.05), Roseburia (P < 0.01), Succinivibrio (P < 0.01) and other genera. The main metabolic pathways such as tryptophan metabolism and steroid hormone biosynthesis in perinatal dairy cows were determined for Saccharomyces cerevisiae and its fermentation products.

介入溶栓治疗LIVCS并发LDVT患者的效果及安全性研究

目的研究介入溶栓治疗左髂静脉受压综合征(LIVCS)并发左下肢深静脉血栓形成(LDVT)患者的效果及安全性。方法选择2020年1月至2023年1月汉中市中心医院收治的90例LIVCS并发LDVT患者作为研究对象,按照随机数表法将患者分为观察组和对照组各45例。对照组患者给予外周静脉溶栓治疗,观察组患者给予介入溶栓治疗。均治疗1周后,比较两组患者的临床疗效、治疗前后的患侧与健侧大小腿周径差、血流速度、受压处血管内径、血栓清除率,并统计两组患者的并发症发生率。结果治疗后,观察组患者的治疗总有效率为97.78%,明显高于对照组的80.00%,差异有统计学意义(P<0.0.5);治疗后,两组患者的患侧与健侧大、小腿周径差均明显降低,且观察组患者患侧与健侧大腿和小腿周径差分别为(1.88±0.43)cm、(1.84±0.36)cm,明显低于对照组的(2.67±0.58)cm、(2.71±0.67)cm,差异均有统计学意义(P<0.05);观察组患者受压处血管内径、血栓清除率分别为(4.57±0.33)mm、(66.74±2.52)%,高于对照组的(4.16±0.23)mm、(61.42±2.31)%,血流速度为(60.18±7.17)cm/s,明显低于对照组的(66.26±7.26)cm/s,差异均有统计学意义(P<0.05);治疗后,观察组患者的并发症发生率为8.89%,明显低于对照组的24.44%,差异有统计学意义(P<0.05)。结论介入溶栓治疗LIVCS并发LDVT疗效确切,可有效改善患者下肢肿胀情况,调节血管狭窄闭塞,降低血栓水平,且并发症发生率低,安全可靠。

CYP1B1遗传多态性与个体化治疗

CYP1B1是细胞色素P450 (cytochrome P450,CYP450s)家族成员,作为I相代谢酶,主要通过催化性激素、脂肪酸、维生素、褪黑素及外源性化学物质和药物的代谢,参与人体组织正常结构和生理功能的维持。研究发现,CYP1B1基因多态性会影响其蛋白表达水平和酶活性,对其催化的代谢过程产生影响,尤其是对性激素和外源物质的代谢过程的改变,与青光眼、各类癌症的发生发展及治疗密切相关。研究CYP1B1参与的机体代谢途径及基因多态性对其影响,有利于深入了解代谢途径的改变在相关疾病发生发展和药物治疗中的作用,加深对癌症、青光眼等疾病的认识,促进从基因层面检测和评估相关疾病的发生风险和预后,从而实现疾病的预防和早期发现,为个体化治疗提供依据,改善患者生存质量,延长生存期。本文拟就近五年CYP1B1参与的主要代谢途径及基因多态性对其影响的研究进展作一综述。

PGC-1α基因多态性与代谢性疾病的相关性

过氧化物酶体增殖物激活受体γ共激活因子1-α(peroxisome proliferator-activated receptor gamma coactivator 1-alpha,PGC-1α)属于人体内一种重要的核转录辅助激活因子,能与多种核受体及转录因子相互作用,以调控其靶基因的表达。PGC1-α在人体能量代谢中发挥着重要的作用,其参与脂肪酸氧化,肝糖异生以及调节线粒体ATP的生物合成等。研究表明PGC-1α基因多态性与Ⅱ型糖尿病、糖尿病肾病、肥胖以及冠心病等代谢性疾病的发生有着密切的关系。探究PGC-1α基因多态性与代谢性疾病的相关性有利于从基因层面检测和评估代谢类疾病的发生风险,从而实现早期发现和预防该疾病的发生,提高代谢性疾病的生存质量,延长生存期。本文拟就近五年PGC-1α参与的代谢途径和其基因多态性与代谢性疾病的相关性的研究进展做一综述。

大腿骨骼肌肌内脂肪组织的含量对肥胖症男性患者糖代谢的 影响

目的·探讨单纯性肥胖症男性患者中大腿骨骼肌肌内脂肪组织(intramuscular adipose tissue,IMAT)含量与糖代谢的相关性。方法·选取2019年12月—2020年12月于上海交通大学医学院附属仁济医院内分泌科肥胖专病门诊就诊的80例单纯性肥胖症男性患者,根据口服葡萄糖耐量试验(oral glucose tolerance test,OGTT)结果将其分为糖耐量正常(normal glucose tolerance,NGT)组与糖调节受损(impaired glucose regulation,IGR)组。收集并比较2组患者的一般资料及实验室检测指标。采用mDixon-Quant技术测量2组患者的大腿各骨骼肌IMAT含量,并对其骨骼肌质子密度脂肪分数(proton density fat fraction,PDFF)进行比较。通过多因素Logistic回归模型分析IGR发生的独立影响因素。结果·与NGT组相比,IGR组患者的腰围较大(P=0.017),谷丙转氨酶、谷草转氨酶、γ-谷氨酰转移酶(γ-glutamyl transferase,GGT)、三酰甘油(triacylglycerol,TAG)、血清游离脂肪酸(nonestesterified fatty acid,NEFA)水平以及缝匠肌PDFF均较高(均P<0.05)。经对年龄、体质量指数、GGT、TAG、NEFA等混杂因素进行校正后,多因素Logistic回归分析的结果显示股外侧肌PDFF、半腱肌PDFF与缝匠肌PDFF是IGR发生的危险因素(均P<0.05)。结论·单纯性肥胖症男性患者中大腿股外侧肌IMAT、半腱肌IMAT、缝匠肌IMAT的含量升高将增加IGR的发生风险。

山西省7条河的流域水生态承载力评估

为科学研判山西省7条河的流域水生态承载现状和存在问题,有效提升河流水生态承载力,构建7条河的流域水生态承载力评估指标体系,应用模糊综合评价法定级评估7条河的流域水生态承载力,使用差分自回归移动平均(autoregressive integrated moving average,ARIMA)模型和长短期记忆(long short-term memory,LSTM)模型对比预测水生态承载力变化趋势。结果表明,2011—2021年,7条河的流域水生态承载力总体为一般超载等级。其中,汾河流域水生态承载力呈基本承载,其他流域为一般超载,水环境指数和水资源指数对7条河的流域水生态承载力影响较大。ARIMA-LSTM模型预测表明:2022—2031年汾河水生态承载力变化呈上升趋势,涑水河、沁(丹)河流域水生态承载力未来趋势存在较大不确定性,其他流域水生态承载力呈不同幅度波动趋势;2031年左右,各流域水生态承载力将得到提升。建议通过提升流域水资源利用率以及加强7条河的流域绿色生态走廊建设来推进流域水生态保护工作。

局部晚期非小细胞肺癌患者血清中Ape1/Ref-1、ICAM-1及IL-17A水平与放射性肺炎发生的相关性研究

背景与目的放射性肺炎主要表现为肺泡上皮及内皮细胞损伤、细胞炎性因子异常表达、纤维母细胞异常增殖及纤维基质合成,其发生与多种细胞因子水平异常相关,这些细胞因子亦可作为预测放射性肺炎发生的生物学指标。本研究评价局部晚期非小细胞肺癌(non-small cell lung cancer,NSCLC)同步放化疗患者放疗前和放疗后血清中脱嘌呤脱嘧啶核酸内切酶1(apurinic/apyrimidinic endonuclease 1/redox factor-1,Ape1/Ref-1)、细胞间粘附分子-1(intercellular adhesion molecules 1,ICAM-1)和白细胞介素-17A(interleukin-17A,IL-17A)水平的变化与放射性肺炎发生的相关性。方法 68例晚期NSCLC患者均行同步放化疗,每位患者正常组织受照剂量相同。在治疗前及治疗后第14周,采用ELISA法检测血清中Ape1/Ref-1、ICAM-1及IL-17A水平。应用美国肿瘤放射治疗协作组和欧洲肿瘤治疗研究协作组(Radiation Therapy Oncology Group/European Organization For Research and Treatment,RTOG/EORTC)诊断分级标准作为急性和晚期肺放射损伤分级标准评价,评价终点≥2级判定为放射性肺炎。结果 18例发生放射性肺炎,50例无放射性肺炎发生。放射性肺炎组放疗前后Ape1/Ref-1水平无显著性变化(P>0.05);放射性肺炎组与非放射性肺炎组Ape1/Ref-1水平无显著性变化(P>0.05)。与放疗前相比,放疗后放射性肺炎组ICAM-1水平上调幅度比非放射性肺炎组ICAM-1水平上调幅度大(P<0.05)。放射性肺炎组患者的IL-17A水平在放疗后显著上升(P<0.05),而非放射性肺炎组IL-17A水平放疗前后水平无显著性变化(P>0.05);放疗后,放射性肺炎组IL-17A水平比非放射性肺炎显著性升高(P<0.05)。相关性分析发现放疗前后ICAM-1的变化与放射性肺炎的发生无明显相关性,而IL-17A的变化与放射性肺炎的发生具有明显相关性。结论晚期NSCLC患者同步放化疗时,在严格限制正常组织受照剂量的同时,血清IL-17A可能是放射性肺炎的预测因子。

基于蛋白芯片技术的多囊卵巢综合征患者卵泡液差异蛋白的研究

目的·运用蛋白芯片技术筛选多囊卵巢综合征(polycystic ovary syndrome,PCOS)患者卵泡液的差异蛋白,为进一步的PCOS研究提供理论依据。方法·采用AAH-BLG-507芯片检测6例正常体质量指数PCOS患者和6例年龄、体质量指数匹配的非PCOS患者的卵泡液,筛选差异蛋白。采用功能注释分析、KEGG通路分析和蛋白互作网络分析方法对差异蛋白进行生物信息学分析。结果·与非PCOS患者相比,PCOS组患者卵泡液中表达上调蛋白25个,表达下调蛋白49个。根据分子功能分类,差异蛋白主要具有细胞因子活性和趋化因子活性。KEGG通路分析提示差异蛋白富集于细胞因子间受体相互作用和趋化因子信号通路。蛋白互作网络分析得到的核心蛋白主要为趋化因子及其受体。结论·运用蛋白芯片技术能够建立PCOS卵泡液差异表达蛋白谱,细胞因子间受体相互作用和趋化因子信号通路可能与PCOS的发病机制有关。