Regulation of different light conditions for efficient biomass production and protein accumulation of Spirulina platensis

Light plays an important role in the photosynthesis and metabolic process of microalgae.However,how different light conditions regulate the biomass production and protein accumulation of microalgae is mostly unknown.In this study,the influence of different light conditions,including light colors,densities,and light:dark cycles on the cell growth and biochemical composition of Spirulina platensis was symmetrically characterized.Under different colored lights,S.platensis all shows an increase trend within the increased light intensity ranges;however,each showing different optimal light intensities.At the same light intensity,different colored lights show different growth rate of S.platensis following the sequence of red>white>green>yellow>blue.The maximum growth rate and protein accumulation were determined as 21.88 and 5.10 mg/(L·d)when illuminated under red LED.The energy efficiency of different light sources was calculated and ranked as red>white>blue≈green>yellow.Transcriptomic analysis suggests that red light can promote cell growth and protein accumulation by upregulating genes related to photosynthesis,carbon fixation,and C-N metabolism pathways.This study provides a conducive and efficient way to promote biomass production and protein accumulation of S.platensis by regulating light conditions.

Erratum to:Regulation of different light conditions for efficient biomass production and protein accumulation of Spirulina platensis

In this article,the legend for Fig.3 f&g was inadvertently mislabeled.The figure below shows the wrong one.The figure should have appeared as shown below.

A Review of Rural Policy-favored Petty Loan Forest Property Certificate Mortgage

Studying petty loan forestry property mortgage mode has significant practical meaning in fund-raising and income-increasing in the forest- agriculture industry,restructuring and expanding the industry as well as setting up scientific industrial public policies. On the basis of literature analysis and field research data,this paper will classify and analyze research papers studying in forestry,property mortgage,and therefore draws conclusions on relevant concepts of petty loan forestry property mortgage,economics and property rights theory as well. At present,the studies of petty loan forestry property mortgage are more practical that are lack of an overall theoretical framework. Considering China's real situation of petty loan forestry property mortgage mode,we have investigated some foreign cases in operating forestry property mortgage systemically and some challenges and difficulties confronted by stakeholders in real cases. Therefore,we put forward that in the current period the fund-raising in forestry industry should focus on establishing and improving an overall rural petty loan forestry property mortgage mode.

Mechanically Inducted Three-Dimensional Hydrogel Fiber Alignment and its Application in Skeletal Muscle Regeneration

Skeletal muscle accounts for 40%~45%of the body weight and is composed of a large number of parallel arrangement of muscle fiber bundles through attaching to the skeleton by the tendon.And robust skeletal muscles provide people the ability of daily activities such as walk,lift and so on.Muscle fibers are generated by multinucleated myotubes which form from the fusion of myoblasts with polarity development,and the synergistic effect between muscle fibers and spontaneous contraction provides support for the movement of the bodies and limbs.However,because of common clinical genetic defect,trauma,tumor,primary myopathy there are so many people cannot exercise like normal people.So it is an urgent task to treat these diseases.Traditional treatment methods are mainly through acupuncture,rehabilitation,drug treatment,etc.But these methods have long treatment times,poor efficacy,and high costs.Recently,the clinical researchers find that these diseases have similar pathological characteristics including structural damage,loss of skeletal muscle function,apoptosis and degradation of muscle fibers Therefore,there is academic and clinical value to study the repair and regeneration of skeletal muscle.Nowadays,tissue engineering provides a new idea for repairing damaged muscle tissue.Tissue engineering uses biological materials as a carrier to regulate the structural properties of materials.By applying biological/chemical/physical stimuli to mimic the microenvironment of the living body.And then construct a living tissue and transplant it into the body for wound repair.With the development of tissue engineering,we regulate the micro-structure of the cell matrix and applying mechanical stimulation to reconstruct a functional muscle microtissue in vitro.Such fabrication and mechanical loading strategy provide an easily adaptable platform to create functional muscle tissue constructs.And the mature muscle microtissues provide a useful tool for the clinical application to treat muscle injury.In this work,we regulate ECM microstructure and apply mechanical stimulation to construct three-dimensional muscle cell mechanical microenvironment.We first regulate the collagen fiber orientation by utilizing the characteristics of collagen gelation,which is a slow process and often need half an hour even more.Before gelation,we fabricate a PDMS mould with rectangle chamber and apply a strain along the long axis to a certain extent utilizing its elasticity.Then we pour collagen solution into the chamber and release the force a few minutes later and gelatinize.After gelation,we apply stretching on the end of the rectangle collagen hydrogel to promote further alignment.We adjust the strain loading time and the strain level to form different groups.Determining the optimal experimental conditions for myotube formation by counting the length and area ofthe myotube and immunofluorescence about relevant proteins.Later,we use western blot and RT-PCR to prove our result.It was found that the application of pre-strain and tensile stimulation can promote the alignment of collagen fibers.Results show that strain loading time and strain extent can regulate the morphology and function of myotubes.The muscle mechanical microenvironment we constructed promotes the formation and activity of polar multinuclear myotubes and builds mature muscle tissue,and determines the related molecular pathways and their transmission mechanisms during the mechanical conduction process.

城乡收入差距的空间相关性及溢出效应分析——以甘肃省为例

缩小城乡收入差距是实现共同富裕的应有之义。本研究采用莫兰指数和空间杜宾模型等方法,探析了2009-2021年甘肃省城乡收入差距时空变化特征和驱动因素。研究表明,2009年至2021年甘肃省各市州城乡收入差距具有正的空间相关性,在空间上呈现较强的集聚现象,存在一定的空间差异性。由空间杜宾固定效应模型及空间溢出效应结果可知,经济、城镇化、交通、环境等都是影响城乡收入差距的重要因素。因此,今后甘肃省在制定发展政策上需要建立多方面的政策配套体系,打破城乡藩篱,因地制宜地进一步支持农业和农村的发展,促进农民增产增收。

Non-contact tensile viscoelastic characterization of microscale biological materials

Many structures and materials in nature and physiology have important ＂meso-scale＂ structures at the micron lengthscale whose tensile responses have proven difficult to characterize mechanically. Although techniques such as atomic force microscopy and micro- and nano-identation are mature for compression and indentation testing at the nano-scale, and standard uniaxial and shear rheometry techniques exist for the macroscale, few techniques are applicable for tensile-testing at the micrometre-scale, leaving a gap in our understanding of hierarchical biomaterials. Here, we present a novel magnetic mechanical testing （MMT） system that enables viscoelastic tensile testing at this critical length scale. The MMT system applies non-contact loading, avoiding gripping and surface interaction effects. We demonstrate application of the MMT system to the first analyses of the pure tensile responses of several native and engineered tissue systems at the mesoscale, showing the broad potential of the system for exploring micro- and meso-scale analysis of structured and hierarchical biological systems.

IR and Raman Spectra Properties of Bi₂O₃-ZnO-B₂O₃-BaO Quaternary Glass System

Among new low-melting-point glasses, bismuth ate glass is deemed to have the most potential as an environmentally friendly replacement for polluting Pb-containing glasses. Current studies of boro-bismuthate glasses focus on the structural influence of the additional oxide in the context of low-melting-point electronic sealing applications. In this study, the structure of quaternary Bi2O3- ZnO-B2O3-BaO glasses was investigated spectroscopic ally, with Fourier-transform-infrared (FT-IR) and Raman spectra recorded for glasses with different main oxide contents. Signals in the FT-IR are mainly observed around 500 cm﹣1, 720 cm﹣1, 840 cm﹣1, 980 - 1080 cm﹣1, and 1200 - 1500 cm﹣1, while the Raman scattering peaks are located at 130 cm﹣1, 390 cm﹣1, 575 cm﹣1, 920 cm﹣1, and 1250 cm﹣1. The glasses are mainly structured around [BO3] units and the numbers of [BiO6] and [BiO3] units increase with the Bi2O3 content increasing. Concurrently, the FT-IR absorption peaks associated with [BO4] units shift to lower wave numbers, indicating a loosening of the glass structure. However, as the B2O3 content is increased, the numbers of [BO3] and [BO4] units increase, while those of [BiO3] and [BiO6] units decrease, highlighting a densification of the glass structure. ZnO acts as a network modifier in these glasses.

Nuclear Mechanotransduction under Geometric Constraint

Cells in vivo reside within a complex microenvironment that is rich in biological,chemical and mechanical cues,playing critical roles in regulating cellular activities(e.g.,proliferation,migration,differentiation)both spatially and temporally.Although it is well accepted that biochemical cues can significantly influence cell functions,accumulating evidence has also shown that mechanical feedback from the cell microenvironment(e.g.,stiffness of ECM,morphology,and tension force)also plays an important role in controlling cell fate.Disequilibrium of the mechanical microenvironment is associated with a series of diseases,such as cancer migration and tissue fibrosis.Thus,there is a pressing need to understand how cells transduce these mechanobiological cues.The cell cytoskeleton is linked to both the nuclear lamina via LINC complexes and to focal adhesions.This enables the intriguing possibility that forces directly transduced by the nucleus might in fact affect gene expression.Can force transmitted to nucleus and associated alterations to the special organization of genome inside the nucleus modulate gene expression programmes and change cell behaviors? This kind of putative mechanotransduction dominated by the nucleus is termed as nuclear mechanotransduction.Evidence shows that isolated nuclei regulate their stiffness to in response to force applied on nesprin with integrated nuclear lamina and emerin required.Another example is that the force applied on integrins in focal adhesions can be transmitted through actin filaments to the LINC complex and then stretch the chromatin directly through lamina-chromatin interactions.However,the mechanism of nuclear mechanotransduction is still unclear.Three hypotheses have been proposed.The first proposed mechanism is that the proteins on the nuclear lamina are phosphorylated induced by force and their special organization is changed to regulate downstream signal transduction.Transcription factors like YAP and calcium ions would enter the nucleus in the context of force stretching the nuclear lamina and opening nuclear pore complexes(NPC)and calcium channels.Another proposed hypothesis in this case is that force propagated through the cytoskeleton stretches,opens or condenses chromatin directly,leading to an entirely different genome organization.Nevertheless,due to the lack of research methods and instruments,researchers have not reached a consensus on how cells sense external forces and react specifically through nucleus.In this study,we used micropatterned techniques to modify poly(N-isopropyl-acrylamide)(PA)hydrogel surface with fibronectin(FN)which promote cell adhesion to shape-engineer the cells to investigate the effects of matrix stiffness on nuclear mechanotransduction.To illustrate the impact on nuclear shape induced by matrix stiffness,the nuclei were stained byDAPI and observed by a laser confocal microscopy with small step sizes.The nuclear shape index(NSI),which indicate the variation of projected nuclear shape was firstly researched thoroughly.Meanwhile,the nuclear height,width and volume were characterized in this study.To investigate the force transmitted to the nuclei in cells cultured on hydrogels with multiple stiffness,the cell traction force was measured and the cytoskeleton like actin cap was studied by pharmacological treatments.We also found that the impacts of matrix stiffness on nuclear mechanics,which indicated by the condensation of chromatin and the overexpression of Lamin A/C.

Genetic Mapping of Laminaria japonica and L. Iongissima Using Amplified Fragment Length Polymorphism Markers in a ＂Two-Way Pseudo- Testcross＂ Strategy

With a ＂two-way pseudo-testcross＂ mapping strategy, we applied the amplified fragment length polymorphism （AFLP） markers to construct two moderate density genetic linkage maps for Laminaria. The linkage maps were generated from the 60 progenies of the F1 cross family （Laminaria iongissima Aresch. × L. Japonica Miyabe） with twenty pairs of primer combinations. Of the 333 polymorphic loci scored in 60 progenies, 173 segregated in a 1：1 ratio, corresponding to DNA polymorphisms heterozygous in a single parent, and the other 58 loci existing in both parents followed a 3：1 Mendelian segregation ratio. Among the loci with 1：1 segregating ratios, 79 loci were ordered in 14 linkage groups （648.6 cM） of the paternal map, and 72 loci were ordered in 14 linkage groups （601.9 cM） of the maternal map. The average density of loci was approximately 1 per 8 cM. To Investigate the homologies between two parental maps, we used 58 loci segregated 3：1 for further analysis, and deduced one homologous linkage group. The linkage data developed in these maps will be useful for detecting loci-controlling commercially important traits for Laminaria.

Hollow TiO2-x porous microspheres composed of wellcrystalline nanocrystals for high-performance lithiumion batteries

Hollow TiO2-x porous microspheres consisted of numerous well-crystalline nanocrystals with superior structural integrity and robust hollow interior were synthesized by a facile sol-gel template-assisted approach and two-step carbonprotected calcination method, together with hydrogenation treatment. They exhibit a uniform diameter of -470 nm with a thin porous wall shell of -50 nm in thickness. The Brunauer-Emmett-Teller （BET） surface area and pore volume are -19 m2/g and 0.07 crnB/g, respectively. These hollow TiOR_x porous microspheres demonstrated excellent lithium storage performance with stable capacity retention for over 300 cycles （a high capacity of 151 mAh/g can be obtained up to 300 cycles at I C, retaining 81.6% of the initial capacity of 185 mAh/g） and enhanced rate capability even up to 10 C （222, 192, 121, and 92.1 mAh/g at current rates of 0.5, 1, 5, and 10 C, respectively）. The intrinsic increased conductivity of the hydrogenated TiO2 microspheres and their robust hollow structure benefidal for lithium ion-electron diffusion and mitigating the structural strain synergistically contribute to the remarkable improvements in their cycling stability and rate performance.

Efficacy and safety of low-dose interleukin-2 in combination with methotrexate in patients with active rheumatoid arthritis:a randomized,double-blind,placebo-controlled phase 2 trial

Rheumatoid arthritis(RA)is an aggressive autoimmune arthritis,and current therapies remain unsatisfactory due to low remission rate and substantially adverse effects.Low-dose interleukin-2(Ld-IL2)is potentially a therapeutic approach to further improve the disease.This randomized,double-blind,placebo-controlled trial was undertaken to evaluate the efficacy and safety of Ld-IL2 in patients with active RA.Patients were randomly assigned(1:1)to receive Ld-IL2,defined as a dose of 1 million IU,or placebo in a 12-week trial with a 12-week follow-up.Three cycles of Ld-IL2 or placebo were administered subcutaneously every other day for 2 weeks(a total of 7 doses),followed by a 2-week break.All patients received a stable dose of methotrexate(MTX).The primary outcomes were the proportion of patients achieving the ACR20,DAS28-ESR<2.6,and the change from baseline in CDAI or SDAI at week 24.Secondary endpoints included other clinical responses and safety.The primary outcomes were achieved in the perprotocol population.The improvements from baseline in CDAI and SDAI were significantly greater across time points for the LdIL2+MTX group(n=17)than for the placebo+MTX group(n=23)(P=0.018 and P=0.015,respectively).More patients achieved ACR20 response in the Ld-IL2+MTX group than those in the placebo+MTX group at week 12(70.6%vs 43.5%)and at week 24(76.5%vs 56.5%)(P=0.014).In addition,low Treg and high IL-21 were associated with good responses to Ld-IL2.Ld-IL-2 treatment was well-tolerated in this study.These results suggested that Ld-IL2 was effective and safe in RA.ClinicalTrials.gov number:NCT 02467504.

Controllable synthesis of highly crystallized mesoporous TiO2/WO3 heterojunctions for acetone gas sensing

Mesoporous semiconducting metal oxides(SMOs)heterojunctions are appealing sensors for gas detecting.However,due to the different hydrolysis and condensation mechanism of every metal precursor and the contradiction between high crystallinity and high surface area,the synthesis of mesoporous SMOs heterojunctions with highly o rdered mesostructures,highly crystallized frameworks,and high surface area remains a huge challenge.In this work,we develop a novel"acid-base pair"adjusted solvent evaporation induced self-assembly(EISA)strategy to prepare highly crystallized ordered mesoporous TiO2/WO3(OM-TiO2/WO3)heterojunctions.The WCl6 and titanium isopropoxide(TIPO)are used as the precursors,respectively,which function as the"acid-base pair",enabling the coassembly with the structure directing agent(PEO-b-PS)into highly ordered meso structures.In addition,PEO-b-PS can be converted to rigid carbon which can protect the meso structures from collapse during the crystallization process.The resultant OM-TiO2/WO3 heterojunctions possess primitive cubic mesostructures,large pore size(~21.1 nm),highly crystalline frameworks and surface area(~98 m2/g).As a sensor for acetone,the obtained OM-TiO2/WO3 show excellent re sponse/recovery perfo rmance(3 s/5 s),good linear dependence,repeatability,selectivity,and long-term stability(35 days).

Effect of grassland degradation on soil quality and soil biotic community in a semi-arid temperate steppe

Grasslands provide a number of ecosystem services for human society.Degradation of grasslands results in the loss of biodiversity and leads to the deterioration of ecosystem functions.In order to accurately assess the influence of grassland degradation on belowground ecosystems,we conducted experiments on a temperate steppe with different levels of degradation and investigated the influence of degradation on soil quality and soil biotic communities.Our results showed that grassland degradation significantly decreased soil quality,with lower values of soil quality index(SQI)observed in the degraded grassland than the meadow steppe and the grassland from the forest-steppe ecotone.Changes in the SQI along the grassland degradation gradient were positively correlated with soil carbon stock and the aboveground biomass,and negatively correlated with the root shoot ratio.Nematode trophic diversity and the ratio of fungal to bacterial PLFA were lower in the degraded grassland than the grassland from the forest-steppe ecotone.The dissimilarities in soil microbial and nematode community composition increased with the changes in soil quality index.Our results indicate that soil quality index based on the minimum data sets could effectively assess the influence of grassland degradation on soil biodiversity and ecosystem function.In order to effectively restore degraded grasslands,the key contributors to the soil quality,such as soil carbon,should be taken on priority basis for revitalizing the soil biodiversity and ecosystem function.

High-sensitive refractive index sensing and excellent slow light based on tunable triple plasmon-induced transparency in monolayer graphene based metamaterial

A patterned monolayer graphene metamaterial structure consisting of six graphene blocks and two graphene strips is proposed to generate triple plasmon-induced transparency(PIT).TriplePIT can be effectively modulated by Fermi levels of graphene.The theoretically calculated results by coupled mode theory show a high matching degree with the numerically simulated results by finite-difference time-domain.Intriguingly,the high-sensitive refractive index sensing and excellent slow-light performance can be realized in the proposed graphene metamaterial structure.The sensitivity(S)and figure of merit can reach up to 5.7115 THz RIU^(-1)and 116.32,respectively.Moreover,the maximum group refractive index is 1036.Hence,these results may provide a new idea for designing graphene-based sensors and slow light devices.

Multi-frequency switch and excellent slow light based on tunable triple plasmon-induced transparency in bilayer graphene metamaterial

We propose a novel bilayer graphene terahertz metamaterial composed of double graphene ribbons and double graphene rings to excite a dynamically adjustable triple plasma-induced transparency(PIT)effect.The coupled mode theory(CMT)is used to explain the PIT phenomenon,and the results of the CMT and the finite-difference time-domain simulation show high matching degree.By adjusting the Fermi levels of graphene,we have realized a pentafrequency asynchronous optical switch.The performance of this switch,which is mainly manifested in the maximum modulation depth(MD=99.97%)and the minimum insertion loss(IL=0.33 dB),is excellent.In addition,we have studied the slow-light effect of this triple-PIT and found that when the Fermi level of graphene reaches 1.2 eV,the time delay can reach 0.848ps.Therefore,this metamaterial provides a foundation for the research of multi-frequency optical switches and excellent slow-light devices in the terahertz band.

Childhood economic status predicting later income： the role of networking ability and encouragement of participation

Drawing from a social capital perspective, this study examines the psychological mechanism and boundary conditions of the relationship between individuals＇ childhood economic status （CES） and later income. Specifically, we tested the mediating role of networking ability and the moderating role of encouragement of participation in this relationship. With a sample of 3635 employees in China and the use of multistage stratified sampling, the results indicate that networking ability mediates the CES and later income relationship. Moreover, encouragement of participation acts as a necessary condition in the relationship between networking ability and later income. Specifically, when encouragement of participation was high, increased networking ability was associated with increased later income. When encouragement of participation was low, the positive relationship between employees＇ networking ability and later income was no longer significant. Finally, the indirect effect of CES on later income mediated by networking ability was stronger for organizations with a higher level of encouragement of participation. Findings and future directions in the theory and practice of career development are discussed.

Identification of lipopolysaccharide-binding protein as a novel citrullinated autoantigen in rheumatoid arthritis

Background:A specific feature of the autoimmune response in rheumatoid arthritis(RA)is the presence of anti-citrullinated protein antibodies(ACPAs)in patient sera.These antibodies can appear several years before disease onset and are involved in the development of RA.Objective:We performed proteomic analysis by mass spectroscopy to identify novel citrullinated antigens and autoantibodies in RA patients.Methods:Polypeptides isolated from the sera of RA patients were identified by Orbitrap high-precision mass spectrometry and then citrulline-containing proteins were selected.The levels of ACPAs against these newly identified citrullinated autoantigens in sera of 100 RA patients and 50 healthy controls were determined by enzyme-linked immunosorbent assays.Results:A total of 135 proteins were identified in RA patients and the protein profile included 11 citrulline-containing antigens.Three of the 11 citrullinated proteins had been reported in previous studies.ACPAs against the novel citrullinated epitopes from these proteins were increased in sera from the RA patients compared with those from healthy controls.Autoantibodies against one of the citrullinated antigens,lipopolysaccharide-binding protein(LBP),was significantly increased in RA patients and associated with disease activities.The titer of anti-citrullinated LBP antibodies(anti-cLBP)was closely related to the infection incidence in RA patients.Conclusion:Serum protein analysis by high-precision proteomic technology is a feasible method to identify novel citrullinated epitopes in RA patients.Anti-cLBP antibodies are associated with disease severity and infection in RA patients.

CEPC Technical Design Report

The Circular Electron Positron Collider(CEPC)is a large scientific project initiated and hosted by China,fostered through extensive collaboration with international partners.The complex comprises four accelerators:a 30 GeV Linac,a 1.1 GeV Damping Ring,a Booster capable of achieving energies up to 180 GeV,and a Collider operating at varying energy modes(Z,W,H,and tt).The Linac and Damping Ring are situated on the surface,while the subterranean Booster and Collider are housed in a 100 km circumference underground tunnel,strategically accommodating future expansion with provisions for a potential Super Proton Proton Collider(SPPC).The CEPC primarily serves as a Higgs factory.In its baseline design with synchrotron radiation(SR)power of 30 MW per beam,it can achieve a luminosity of 5×10^(34)cm^(-2)s^(-1)per interaction point(IP),resulting in an integrated luminosity of 13 ab^(-1)for two IPs over a decade,producing 2.6 million Higgs bosons.Increasing the SR power to 50 MW per beam expands the CEPC's capability to generate 4.3 million Higgs bosons,facilitating precise measurements of Higgs coupling at sub-percent levels,exceeding the precision expected from the HL-LHC by an order of magnitude.This Technical Design Report(TDR)follows the Preliminary Conceptual Design Report(Pre-CDR,2015)and the Conceptual Design Report(CDR,2018),comprehensively detailing the machine's layout,performance metrics,physical design and analysis,technical systems design,R&D and prototyping efforts,and associated civil engineering aspects.Additionally,it includes a cost estimate and a preliminary construction timeline,establishing a framework for forthcoming engineering design phase and site selection procedures.Construction is anticipated to begin around 2027-2028,pending government approval,with an estimated duration of 8 years.The commencement of experiments and data collection could potentially be initiated in the mid-2030s.

硫铁矿尾矿中铜的硫酸浸出

采用酸浸法提取硫铁矿尾矿中有价元素铜。研究了硫酸质量分数、浸出时间、矿石粒度、矿石投加量、浸出温度和转速对铜浸出的影响。在单因素实验的基础上,采用正交实验法对浸出工艺条件进行优化。采用扫描电镜(SEM)和能谱仪(EDS)对酸浸前后尾矿表面的微观形貌进行了分析,初步探讨了酸浸反应后期抑制Cu浸出的影响机制。结果表明,酸浸铜的最佳工艺条件为:硫酸质量分数30%、浸出时间6 h、矿石粒度150μm、矿石用量5 g、浸出温度108℃、转速440 r/min,在此条件下,铜的浸出率为50.68%。扫描电镜和能谱分析表明,浸出残渣表面被微米级的二氧化硅颗粒紧密包裹,钝化了后期的浸出反应。

危机下的组织沟通网络变化与知识分享行为演变:一项基于工作系统大数据的追踪研究

随着互联网技术的普及,企业行为越来越多地发生和存储于网络当中,如何对工作网络中的动态数据进行有效利用和分析成为AI时代管理研究的热点和难点。另一方面,当下中国企业面临着前所未有的风险挑战和环境变化,有效应对不确定性和环境变化也成为核心命题。由此,探索企业应对危机的有效管理对策和行为规律显得尤为重要。本研究以高新科技企业M公司为对象,针对该企业在2021年初遭遇的一次重大危机事件进行了为期两个月的追踪研究。通过记录公司的具体管理动作,抽取工作系统内的沟通网络平台和知识管理平台大数据来进行分析,尝试构建企业实施危机管理的动态过程与有效机制,最后采用深度访谈对危机管理效果进行了评估。研究结合大数据分析技术与定性研究方法 ,更为全面地描述了危机发生后企业管理动作与沟通网络变化、知识分享行为演变之间的动态规律,揭示出企业危机管理的有效路径,既丰富了危机管理领域的实证研究,也是将新技术应用于案例研究的一次大胆尝试。