黄瓜CsaDMR6-2基因的克隆及特征分析

通过系统进化树和黄瓜霜霉病主效QTL分析,确定了与拟南芥抗霜霉病突变基因DMR6同源性较高的Csa DMR6-2基因为黄瓜霜霉病感病候选基因。以15份不同遗传背景的抗病和感病黄瓜自交系为材料克隆了Cs DMR6-2基因,经过与已知功能的拟南芥基因DMR6、DLO1和DLO2的氨基酸进行多序列比对、蛋白质二级和三级结构的预测,结果显示:欧洲温室型的抗病材料K15和K58的Csa DMR6-2的CDS序列在856bp处有1个碱基的突变,引起相应氨基酸由丝氨酸(TCA)变成丙氨酸(GCA),进而引起其部分蛋白质二级和三级结构的变化;Csa DMR6-2与拟南芥的DMR6、DLO1和DLO2基因具有很高的同源性,且同属于一类氧化酶,两者具有相同的结构域,而抗病材料的变异位点均发生在催化区。试验结果为进一步研究Csa DMR6-2的基因功能和利用感病基因获得霜霉病持久广谱抗性打下了良好的基础。

Breeding melon(Cucumis melo)with resistance to powdery mildew and downy mildew

Melon(Cucumis melo L.)production is often restricted by a plethora of pests and diseases,including powdery mildew and downy mildew caused respectively by the fungal species Podosphaera xanthii/Golovinomyces orontii and oomycete species Pseudoperonospora cubensis.Many efforts have been directed on identification of resistant sources by screening(wild)melon germplasm.In the current review,we summarized such efforts from various publications of the last 50 plus years.Resistance to powdery mildew has been identified in 239 melon accessions and downy mildew resistance in 452 accessions of both C.melo and the wild relative species C.figarei.Among the resistance sources,C.melo var.cantalupensis accessions PMR 45,PMR 5,PMR 6,and WMR 29 as well as C.melo var.momordica accessions PI 124111,PI 124112,and PI 414723 have been considered as the most valuable germplasm because multiple resistance genes have been identified from these accessions and are widely used in melon resistance breeding.Further genetic mapping in a number of resistant sources has enabled identification of 25 dominant genes,two recessive genes and seven QTLs conferring powdery mildew resistance,as well as eight dominant genes and 11 QTLs for downy mildew resistances.Based on the reported sequences of associated markers,we anchored physically(many of)these genes and QTLs to chromosomes of the melon cv.DHL92 genome.In addition to presenting a comprehensive overview on powdery mildew and downy mildew resistance in(wild)melon germplasm,we suggest strategies aiming at breeding melon with durable and broad-spectrum resistance to pathogens and pests.

Expression and role of PTV1 lncRNA in glioma cells progression

Objective The aim of this study was to investigate the expression of PTV1 lncRNA in gliomas and themechanism of its interaction with miR-203a.Methods U87 and U251 cells were cultured stably and transfected with sh-PTV1 or ov-PTV1, respectively.The proliferative activity of U87 and U251 cells was detected and the transplanted tumor model nude micewere divided into U87 and U251 groups. U87-sh and u251-ov cells were injected into the armpit, thenmiR-203a mic and miR-203a inhibitors were administered to detect the changes in the expression of tumorrelatedproteins.Results The relative expression of PTV1 in gliomas was significantly higher than that in normal braintissues, while in GBM it was significantly higher than that in low-grade gliomas. Knockdown of PTV1significantly inhibited the proliferation of U87 cells, resulting in fewer cell clones;overexpression of rPTV1significantly promoted the proliferation of U251 cells, resulting in more cell colonies. The dual LuciferaseReporter assay showed that SP2 was a potential target of miR-203a. When U87 cells were treated with amiR-203a mimic, the expression of SP2 decreased;and when U251 cells were treated with a miR-203ainhibitor, the expression of SP2 increased significantly. SP2 was overexpressed in u87-sh cells and theproliferation, migration, and invasion of u87-sh cells were significantly enhanced. U251-ov cells showedthe opposite trend. Compared with the control group mice, the tumor volume in u87-sh group mice wassignificantly smaller and the positive rate of SP2 in tumor tissue was significantly lower. After administrationof the miR-203a inhibitor, the tumor volume increased gradually and the positive rate of SP2 increasedsignificantly, while u251-ov mice showed the opposite trend.Conclusion lncRNA PTV1 can be used as a molecule to interfere with miR-203a expression in order todownregulate SP2 and to promote the proliferation and invasion of glioma cells. lncRNA PTV1 may be anew biomarker and therapeutic target for glioma.

Fashion Shopping Value in WeChat Moments

Fashion shopping in WeChat(微信)Moments(朋友圈)has become an important new channel because of the rapid development and broad application of the smart phone technology.The purpose of the paper is to test a model dealing with direct effects of shopping values on purchase intention.The research model was tested using smart phone in a survey of 276 respondents.The results as follows:First,higher shopping value perceived consumers have more purchase intention of fashion products in WeChat.Second,utilitarian shopping value play more positive impact on purchase intention than hedonic shopping value.Additional managerial implications are discussed.

蒙药明目十一味丸对糖尿病视网膜病变Ⅰ期视网膜功能的影响

目的:评价蒙药明目十一味丸治疗糖尿病视网膜病变(DR)Ⅰ期的临床疗效。方法:前瞻性临床研究。选取2016年7月至2017年3月在内蒙古民族大学附属医院眼科就诊的DRⅠ期患者120例(120眼),通过随机数字表法将其分成蒙药组和对照组各60例,蒙药组加用明目十一味丸,对照组加用羟苯磺酸钙胶囊,一个疗程28d,共3个疗程。分别在治疗前后对2组患者进行多焦视网膜电图(mfERG)及超广角眼底照相检查,记录N1区域和P1区域的潜伏期、振幅变化及视网膜微动脉瘤和出血点的数目。采用t检验、χ^2检验对数据进行分析。结果:对照组和蒙药组在治疗前后mfERG测得的R1~R5P1区域的振幅密度值差异有统计学意义(t对照组=66.86、18.05、36.33、47.43、30.56,P<0.001;t蒙药组=31.52、54.56、101.98、127.02、45.74,P<0.001),N1区域的振幅密度值差异有统计学意义(t对照组=70.18、47.02、78.08、57.44、64.51,P<0.001;t蒙药组=46.09、140.47、145.14、50.46、73.94,P<0.001)。与对照组相比,蒙药组治疗前后R1~R5P1、R1~R3N1区域的振幅密度值差值差异有统计学意义(tP1=8.96、23.96、25.10、28.80、13.67,P<0.001;tN1=9.38、30.34、52.06,P<0.001),R4~R5N1区域的振幅密度值差值差异无统计学意义。2组治疗前后潜伏期比较,R1~R5P1区域、N1区域的差异均无统计学意义。2组治疗前后微动脉瘤及出血点数目比较,差异有统计学意义(t=2.08,P=0.042;t=2.07,P=0.043)。眼底照相蒙药组与对照组有效率分别达88%、73%,差异有统计学意义(χ^2=4.36,P=0.037)。结论:蒙药明目十一味丸能明显改善Ⅰ期DR视网膜功能的损伤,疗效显著,可作为治疗DRⅠ期的常规用药。