Osteoarthritis (OA) is a degenerative joint disease and a major cause of pain and disability in older adults. We have previously identified epidermal growth factor receptor (EGFR) signaling as an important regulat...Osteoarthritis (OA) is a degenerative joint disease and a major cause of pain and disability in older adults. We have previously identified epidermal growth factor receptor (EGFR) signaling as an important regulator of cartilage matrix degradation during epiphyseal cartilage development. To study its function in OA progression, we performed surgical destabilization of the medial meniscus (DMM) to induce OA in two mouse models with reduced EGFR activity, one with genetic modification (, was/+ mice) and the other one with pharmacological inhibition (gefitinib treatment). Histological analyses and scoring at 3 months post-surgery revealed increased cartilage destruction and accelerated OA progression in both mouse models. TUNEL staining demonstrated that EGFR signaling protects chondrocytes from OA-induced apoptosis, which was further confirmed in primary chondrocyte culture. Immunohistochemistry showed increased aggrecan degradation in these mouse models, which coincides with elevated amounts of ADAMTS5 and matrix metalloproteinase 13 (MMP13), the principle proteinases responsible for aggrecan degradation, in the articular cartilage after DMM surgery. Furthermore, hypoxia-inducible factor 2α (HIF2α), a critical catabolic transcription factor stimulating MMP13 expression during OA, was also upregulated in mice with reduced EGFR signaling. Taken together, our findings demonstrate a primarily protective role of EGFR during OA progression by regulating chondrocyte survival and cartilage degradation.展开更多
An entropy-based statistic TpE has been proposed for genomic association study for disease-susceptibility locus. The statistic TpE may be directly adopted and/or extended to quantitative-trait locus (QTL)mapping for...An entropy-based statistic TpE has been proposed for genomic association study for disease-susceptibility locus. The statistic TpE may be directly adopted and/or extended to quantitative-trait locus (QTL)mapping for quantitative traits. In this article, the statistic TpE was extended and applied to quantitative trait for association analysis of QTL by means of selective genotyping. The statistical properties (the type I error rate and the power) were examined under a range of parameters and population-sampling strategies (e.g., various genetic models, various heritabilities, and various sample-selection threshold values) by simulation studies. The results indicated that the statistic TpE is robust and powerful for genomic association study of QTL. A simulation study based on the haplotype frequencies of 10 single nucleotide polymorphisms (SNPs) of angiotensin-I converting enzyme genes was conducted to evaluate the performance of the statistic TPE for genetic association study.展开更多
This paper considers the problem of delay-dependent exponential stability in mean square for stochastic systems with polytopic-type uncertainties and time-varying delay. Applying the descriptor model transformation an...This paper considers the problem of delay-dependent exponential stability in mean square for stochastic systems with polytopic-type uncertainties and time-varying delay. Applying the descriptor model transformation and introducing free weighting matrices, a new type of Lyapunov-Krasovskii functional is constructed based on linear matrix inequalities (LMIs), and some new delay-dependent criteria are obtained. These criteria include the delay-independent/rate- dependent and delay-dependent/rate-independent exponential stability criteria. These new criteria are less conservative than existing ones. Numerical examples demonstrate that these new criteria are effective and are an improvement over existing ones.展开更多
Background The prevalence of Alzheimer's disease(AD)comorbid with depression is common.However,the mechanisms of AD with depression remain unclear.Aims To investigate the regional alterations of brain activity of ...Background The prevalence of Alzheimer's disease(AD)comorbid with depression is common.However,the mechanisms of AD with depression remain unclear.Aims To investigate the regional alterations of brain activity of AD with depression in resting-state functional magnetic resonance imaging(rs-fMRI).Methods 154 patients with AD who met the inclusion criteria were recruited from the Zhejiang Provincial People’s Hospital from October 2014 to October 2016.According to whether the core symptoms of depression were present,patients were divided into two groups,22 patients with AD with depression(AD-D)and 52 patients with AD without depression(AD-nD).The amplitude of low frequency fluctuations(ALFF)was compared between two groups by performing independent-samples f-test.Results Compared with the AD-D group,increased ALFF values in the bilateral superior frontal gyrus,left middle frontal gyrus and left inferior frontal gyrus were observed in the AD-nD group.The brain activity in the AD-nD group in the bilateral superior frontal gyrus,left middle frontal gyrus and the left inferior frontal gyrus was higher than the AD-D group.Conclusions Resting-state brain functional alterations may be closely bound up with the pathophysiologic features of patients with AD with depressive symptoms.展开更多
A precedence order is defined based on the release dates of jobs' direct successors. Using the defined precedence order and Heap Sort, a new polynomial algorithm is provided which aims to solve the parallel schedulin...A precedence order is defined based on the release dates of jobs' direct successors. Using the defined precedence order and Heap Sort, a new polynomial algorithm is provided which aims to solve the parallel scheduling problem P|pj = 1, r j, outtree| ∑Cj Cj. The new algorithm is shown to be more compact and easier to implement.展开更多
Straw mulching is a widespread practice for reducing the soil carbon loss caused by erosion.However,the effects of straw mulching on dissolved organic matter(DOM)runoff loss from black soil are not well studied.How st...Straw mulching is a widespread practice for reducing the soil carbon loss caused by erosion.However,the effects of straw mulching on dissolved organic matter(DOM)runoff loss from black soil are not well studied.How straw mulching affects the composition and loss of runoff DOM by changing soil aggregates remains largely unclear.Here,a straw mulching treatment was compared to a no mulching treatment(as a control)on sloping farmland with black soil erosion in Northeast China.We divided the soil into large macroaggregates(>2 mm),small macroaggregates(0.25-2 mm),and microaggregates(<0.25 mm).After five rain events,the effects of straw mulching on the concentration(characterized by dissolved organic carbon(DoC)and composition(analyzed by fluorescence spectroscopy)of runoff and soil aggregate DOM were studied.The results showed that straw mulching reduced the runoff amount by 54.7%.Therefore,although straw mulching increased the average DOc concentration in runoff,it reduced the total runoff DOM loss by 48.3%.The composition of runoff DOM is similar to that of soil,as both contain humic-like acid and protein-like components.With straw mulching treatment,the protein-like components in small macroaggregates accumulated and the protein-like components in runoff declined with rain events.Fluorescence spectroscopy technology may help in understanding the hydrological paths of rain events by capturing the dynamic changes of runoff and soil DOM characteristics.A variation partitioning analysis(VPA)indicated that the DOM concentration and composition of microaggregates explained 68.2%of the change in runoff DOM from no mulching plots,while the change in runoff DOM from straw mulching plots was dominated by small macroaggregates at a rate of 55.1%.Taken together,our results demonstrated that straw mulching reduces the fragmentation of small macroaggregates and the loss of microaggregates,thus effecting DOM compositions in soil and reducing the DOM loss in runoff.These results provide a theoretical basis for reducing carbon loss in sloping farmland.展开更多
To effectively remove n-propylbenzene(n-PBZ)and isopropylbenzene(i-PBZ)leaked into seawater using Rhinomonas reticulata S6A(a newly isolated marine microalga),the effects of three inorganic nutrients and four environm...To effectively remove n-propylbenzene(n-PBZ)and isopropylbenzene(i-PBZ)leaked into seawater using Rhinomonas reticulata S6A(a newly isolated marine microalga),the effects of three inorganic nutrients and four environmental factors on their degradation were determined after 7 d of inoculation.Results show that NaNO_(3) at 300 mg/L caused a higher removal efficiency of both n-PBZ and i-PBZ(44.79%and 39.26%),while for NaH_(2) PO_(4)·H_(2) O,greater removal rates of two PBZs(47.30%and 42.23%)were achieved at 30 and 20 mg/L,respectively.NaHCO_(3) supplementation(500-750 mg/L)resulted in a large reduction(43.67%-45.04%)in i-PBZ concentration.The change in seawater pH(from 6 to 9)did not affect the elimination of n-PBZ and i-PBZ.The most suitable salinity and temperature were 30 and 25-30℃,respectively,leading to the PBZs removal of~40%.Light intensity exhibited significant influence on elimination of PBZs,and the maximum removal efficiencies of 56.07%(n-PBZ)and 55.00%(i-PBZ)were recorded under 200 and 600μmol/(m^(2)·s),respectively.In addition,the microalga could still remove PBZs when it failed to grow well due to darkness,strong light,low temperature,or low salinity,which might mean that good growth of alga is not always a necessary condition for PBZs removal.Therefore,attention should be paid to the suitability of nutrient levels and environmental conditions(excluding pH)in seawater when using microalgae for bioremediating PBZs-contaminated seawater.展开更多
Ribosomopathies encompass a spectrum of disorders arising from impaired ribosome biogenesis and reduced functionality.Mutation or dysexpression of the genes that disturb any finely regulated steps of ribosome biogenes...Ribosomopathies encompass a spectrum of disorders arising from impaired ribosome biogenesis and reduced functionality.Mutation or dysexpression of the genes that disturb any finely regulated steps of ribosome biogenesis can result in different types of ribosomopathies in clinic,collectively known as ribosomopathy genes.Emerging data suggest that ribosomopathy patients exhibit a significantly heightened susceptibility to cancer.Abnormal ribosome biogenesis and dysregulation of some ribosomopathy genes have also been found to be intimately associated with cancer development.The correlation between ribosome biogenesis or ribosomopathy and the development of malignancies has been well established.This work aims to review the recent advances in the research of ribosomopathy genes among human cancers and meanwhile,to excavate the potential role of these genes,which have not or rarely been reported in cancer,in the disease development across cancers.We plan to establish a theoretical framework between the ribosomopathy gene and cancer development,to further facilitate the potential of these genes as diagnostic biomarker as well as pharmaceutical targets for cancer treatment.展开更多
The MYB transcription factor is one of the largest gene families in plants,playing an important role in regulating plant growth,development,response to stress,senescence,and especially the anthocyanin biosynthesis.In ...The MYB transcription factor is one of the largest gene families in plants,playing an important role in regulating plant growth,development,response to stress,senescence,and especially the anthocyanin biosynthesis.In this study,A total of 217 MYB genes,including 901R-MYBs,124 R2R3-MYBs,and 3 R1R2R3-MYBs have been identified from the potato genome.The 1R-MYB and R2R3-MYB family members could be divided into 20 and 35 subgroups respectively.Analysis of gene structure and protein motifs revealed that members within the same subgroup presented similar exon/intron and motif organization,further supporting the results of phylogenetic analysis.Potato is an ideal plant to reveal the tissue-specific anthocyanins biosynthesis regulated by MYB,as the anthocyanins could be accumulated in different tissues,showing colorful phenotypes.Five pairs of colored and colorless tissues,stigma,petal,stem,leaf,and tuber flesh,were applied to the transcriptomic analysis.A total of 70 MYB genes were found to be differentially expressed between colored and colorless tissues,and these differentially expressed genes were suspected to regulate the biosynthesis of anthocyanin of different tissues.Co-expression analysis identified numerous potential interactive regulators of anthocyanins biosynthesis,involving 39 MYBs,24 bHLHs,2 WD-repeats,and 29 biosynthesis genes.Genome-wide association study(GWAS)of tuber flesh color revealed amajor signal at the end of Chromosome 10,which was co-localized with reported I gene(StMYB88),controlling tuber peel color.Analyses of DEGs(Differentially Expression Genes)revealed that both StMYB88 and StMYB89 were closely related to regulating anthocyanin biosynthesis of tuber flesh.This work offers a comprehensive overview of the MYB family in potato and will lay a foundation for the functional validation of these genes in the tissue-specific regulation of anthocyanin biosynthesis.展开更多
Objective: Cancer metastasis remains the primary cause of cancer-related death worldwide.In a previous study, we found that levels of BTB/POZ domain-containing protein KCTD12 are lower in metastatic melanoma cells tha...Objective: Cancer metastasis remains the primary cause of cancer-related death worldwide.In a previous study, we found that levels of BTB/POZ domain-containing protein KCTD12 are lower in metastatic melanoma cells than in parental melanoma cells.The purpose of this study was to identify the roles of KCTD12 in cancer metastasis.Methods: The Cancer Genome Atlas(TCGA) datasets were used to evaluate the relationship between KCTD12 and skin cutaneous melanoma(SKCM) prognosis.The effects of endogenous KCTD12 on biological behaviors were examined using the MTT assay.The impacts of KCTD12 on melanoma stemness were explored using spheroid formation assay.KCTD12 knockout A375 cells were generated to confirm the inhibitory effect of KCTD12 on CD271, and a mouse metastatic model was used to determine the impact of KCTD12 on melanoma metastasis in vivo.Results: KCTD12 levels were lower in lung metastatic cells than in paired parental melanoma cells, and low KCTD12 expression indicated a poor prognosis in SKCM.Cancer metastasis-related capacities were higher in lung metastatic cells than in parental melanoma cells.Moreover, KCTD12 knockdown enhanced tumor growth and metastasis both in vitro and in vivo.Mechanistically, the interaction between KCTD12 and CD271 might be responsible for the stemness transformation after KCTD12 knockdown.Conclusions: This study identifies for the first time the role of the interaction between KCTD12 and CD271 in inducing melanoma cell stemness transformation.Moreover, KCTD12 repression enhances melanoma cell growth, adhesion, migration and invasion.展开更多
This present study was aimed to investigate the roles of the receptors of Thl/Th2 cytokines and chemokines in lhe pathogenesis of chronic idiopathic urticaria (CIU). Thirty patients with CIU, 30 patients with dermog...This present study was aimed to investigate the roles of the receptors of Thl/Th2 cytokines and chemokines in lhe pathogenesis of chronic idiopathic urticaria (CIU). Thirty patients with CIU, 30 patients with dermographism and 30 healthy controls were randomly enrolled. Reverse transcription-PCR (RT-PCR) was used to analyze the mRNA of cytokine receptors in peripheral blood mononuclear cells (PBMCs). The mRNA levels of tumor necro- sis factor receptor (TNFR), interferon-7 receptor (IFN-yR), and interleukin-10 receptor (IL-IOR) were statistically increased in the CIU group (P 〈 0.05), while IL-2R, IL-4R, IL-6R, and IL-13R showed no significant differences between the CIU and other groups. The mRNA levels of CCR3 and CCR6 were statistically increased in the CIU group (P 〈 0.05). The toll-like receptor 2 (TLR2) mRNA level was significantly lower in the CIU group than the healthy control group (P 〈 0.05). These findings indicate that the regulation of mRNA of TNFR, IFN-γR, IL-IOR, CCR3, CCR6 and TLR2 may be involved in the pathogenesis of CIU.展开更多
Objective:To understand the rate of cognition and protection of COVID-19 in a community in Hubei province.Methods:The related knowledge,epidemic situation and self-protective measures of COVID-19 were investigated by ...Objective:To understand the rate of cognition and protection of COVID-19 in a community in Hubei province.Methods:The related knowledge,epidemic situation and self-protective measures of COVID-19 were investigated by mobile phone questionnaire among 836 people in a community in Hubei province.Results:The rate of the awareness of COVID-19’s relevant knowledge and the ways to obtain the epidemic situation varied among different age groups.After multi-channel intervention in the community,the rate of the awareness of COVID-19’s relevant knowledge increased from 75.0%to 96.2%,and the implementation rate of personal protection increased from 59.6%to 92.6%.Conclusion:It is of great significance to improve residents’awareness and protection consciousness and block the spread of COVID-19 in the community through multi-channel intervention.展开更多
Climate and land-use changes are predicted to impact biodiversity,threatening ecosystem services and functions.However,their combined effects on the functional diversity of mammals at the regional scale remain unclear...Climate and land-use changes are predicted to impact biodiversity,threatening ecosystem services and functions.However,their combined effects on the functional diversity of mammals at the regional scale remain unclear,especially at the beta level.Here,we use projected climate and land-use changes in China to investigate their potential effects on the alpha and beta functional diversities of terrestrial mammals under low-and high-emission scenarios.In the current projection,we showed strong positive spatial correlations between functional richness and species richness.Functional evenness(FEve),functional specialization(FSpe),and functional originality(FOri)decreased with species richness,and functional divergence(FDiv)increased first and then plateaued.Functional beta diversity was dominated by its nestedness component,in contrast to the taxonomic facet.Potential changes in species richness are more strongly influenced by land-use change under the low-emission scenario,while under the high-emission scenario,they are more strongly influenced by climate change.Changes in functional richness(FRic)were inconsistent with those in species richness,with a magnitude of decreases greater than predicted from species richness.Moreover,mammal assemblages showed potential functional differentiation(FD)across the country,and the trends exceeded those towards taxonomic differentiation(TD).Our findings help us understand the processes underlying biodiversity responses to global changes on multiple facets and provide new insight for conservation plans.展开更多
Background:Atopic dermatitis(AD)affects approximately 10%of adults worldwide.CM310 is a humanized monoclonal antibody targeting interleukin-4 receptor alpha that blocks interleukin-4 and interleukin-13 signaling.This ...Background:Atopic dermatitis(AD)affects approximately 10%of adults worldwide.CM310 is a humanized monoclonal antibody targeting interleukin-4 receptor alpha that blocks interleukin-4 and interleukin-13 signaling.This trial aimed to evaluate the efficacy and safety of CM310 in Chinese adults with moderate-to-severe AD.Methods:This multicenter,randomized,double-blind,placebo-controlled,phase 2b trial was conducted in 21 medical institutions in China from February to November 2021.Totally 120 eligible patients were enrolled and randomized(1:1:1)to receive subcutaneous injections of 300 mg CM310,150 mg CM310,or placebo every 2 weeks for 16 weeks,followed by an 8-week follow-up period.The primary endpoint was the proportion of patients achieving≥75%improvement in the Eczema Area and Severity Index(EASI-75)score from baseline at week 16.Safety and pharmacodynamics were also studied.Results:At week 16,the proportion of EASI-75 responders from baseline was significantly higher in the CM310 groups(70%[28/40]for high-dose and 65%[26/40]for low-dose)than that in the placebo group(20%[8/40]).The differences in EASI-75 response rate were 50%(high vs.placebo,95%CI 31%-69%)and 45%(low vs.placebo,95%CI 26%-64%),with both P values<0.0001.CM310 at both doses also significantly improved the EASI score,Investigator’s Global Assessment score,daily peak pruritus Numerical Rating Scale,AD-affected body surface area,and Dermatology Life Quality Index compared with placebo.CM310 treatment reduced levels of thymus and activation-regulated chemokine,total immunoglobulin E,lactate dehydrogenase,and blood eosinophils.The incidence of treatment-emergent adverse events(TEAEs)was similar among all three groups,with the most common TEAEs reported being upper respiratory tract infection,atopic dermatitis,hyperlipidemia,and hyperuricemia.No severe adverse events were deemed to be attributed to CM310.Conclusion:CM310 at 150 mg and 300 mg every 2 weeks demonstrated significant efficacy and was well-tolerated in adults with moderate-to-severe AD.Trial Registration:ClinicalTrials.gov,NCT04805411.展开更多
This study examined how a dialogic approach in an online media literacy class at a university in China helped to develop college students’global awareness when the world was disrupted by the coronavirus disease(COVID...This study examined how a dialogic approach in an online media literacy class at a university in China helped to develop college students’global awareness when the world was disrupted by the coronavirus disease(COVID-19).Using writing exemplars from students’online dialogues and reflective journals,this article demonstrates the potentialities of an online dialogic approach to guide a sense of togetherness and critical solidarity.The digital dialogical approach provides an expanded space for students to converse with multiple voices,meditate on tensions,and rethink their own stances as citizens of their country and the world.The article also underscores the role of higher education in cultivating a sense of global community among the younger generation and bridging the ideological divide in society.展开更多
ABO_(3)-type perovskite oxides with abounding defect structures and diverse physical chemistry attributes have been extensively studied in heterogeneous catalysis.Semiconductor LaFeO_(3)perovskites fabricated via the ...ABO_(3)-type perovskite oxides with abounding defect structures and diverse physical chemistry attributes have been extensively studied in heterogeneous catalysis.Semiconductor LaFeO_(3)perovskites fabricated via the sol-gel method were used as peroxydisulfate(PDS)activators for methylene blue(MB)degradation under low-intensity ultra-violet(UV)-light to evaluate the degradation efficiency of organic pollutants and mechanism.The results indicate that under low-intensity UV irradiation,the developed UV/LaFeO_(3)/PDS system shows excellent degradation ability of organic pollutants in a wide pH range.Electron spin resonance and radical quenching experiments verify that SO_(4)^(·-),·OH,h^(+)and ^(1)O_(2)are generated during the activation process,and ^(1)O_(2)plays a dominant role in MB degradation.Reduction of low oxidation state Fe(Ⅱ)and O_(2)^(2-)/O^(-)on the LaFeO_(3)surface shows that oxygen vacancy,as the electron transfer mediator,enhances the redox cycle efficiency of Fe(Ⅱ)/Fe(Ⅲ).Photogenerated electrons of LaFeO_(3)involved in the cyclic transformation of Fe(Ⅱ)/Fe(Ⅲ)and PDS activation further increase the number of active radicals and thus promote the synergistic effect of photocatalytic coupled with sulfate radicalbased advanced oxidation processes.展开更多
Dear Editor,Metastasis is the cause of most fatalities in cancer patients and remains the phenomenon poorly understood mechanistically. Deciphering of the regulatory networks underlying the cancer cell metastasis is u...Dear Editor,Metastasis is the cause of most fatalities in cancer patients and remains the phenomenon poorly understood mechanistically. Deciphering of the regulatory networks underlying the cancer cell metastasis is urgently needed. Nucleolar protein 7 (NOL7) has been reported to function as a tumor suppressor in cervical cancer.1 Our current study reveals a novel tumor-promoting capacity of NOL7 in melanoma. We first detected that NOL7 expression is upregulated in metastatic melanoma as compared with its expression at the primary site through isobaric tag for relative and absolute quantitation proteomic screening, further confirming this finding with the analysis of NOL7 protein and messenger RNA (mRNA) levels (Supplementary Fig. S1). Importantly, NOL7 expression increased with the disease progression from benign nevus to primary melanoma and further to metastatic melanoma (Fig. 1a and Supplementary Fig. S1d). Previous studies have shown that melanoma is commonly associated with the amplification of the chromosome region 6p, particularly 6p21–23, where the NOL7 gene resides, and that this region frequently undergoes heterozygous loss in cervical cancer.2 It might therefore be predicted that NOL7 exhibits a different expression pattern and plays different roles in melanoma and cervical cancer.展开更多
基金supported by ASBMR Research Career Enhancement Award (to LQ)NIH grants AR060991 (to LQ)AR062908 (to ME-I)
文摘Osteoarthritis (OA) is a degenerative joint disease and a major cause of pain and disability in older adults. We have previously identified epidermal growth factor receptor (EGFR) signaling as an important regulator of cartilage matrix degradation during epiphyseal cartilage development. To study its function in OA progression, we performed surgical destabilization of the medial meniscus (DMM) to induce OA in two mouse models with reduced EGFR activity, one with genetic modification (, was/+ mice) and the other one with pharmacological inhibition (gefitinib treatment). Histological analyses and scoring at 3 months post-surgery revealed increased cartilage destruction and accelerated OA progression in both mouse models. TUNEL staining demonstrated that EGFR signaling protects chondrocytes from OA-induced apoptosis, which was further confirmed in primary chondrocyte culture. Immunohistochemistry showed increased aggrecan degradation in these mouse models, which coincides with elevated amounts of ADAMTS5 and matrix metalloproteinase 13 (MMP13), the principle proteinases responsible for aggrecan degradation, in the articular cartilage after DMM surgery. Furthermore, hypoxia-inducible factor 2α (HIF2α), a critical catabolic transcription factor stimulating MMP13 expression during OA, was also upregulated in mice with reduced EGFR signaling. Taken together, our findings demonstrate a primarily protective role of EGFR during OA progression by regulating chondrocyte survival and cartilage degradation.
文摘An entropy-based statistic TpE has been proposed for genomic association study for disease-susceptibility locus. The statistic TpE may be directly adopted and/or extended to quantitative-trait locus (QTL)mapping for quantitative traits. In this article, the statistic TpE was extended and applied to quantitative trait for association analysis of QTL by means of selective genotyping. The statistical properties (the type I error rate and the power) were examined under a range of parameters and population-sampling strategies (e.g., various genetic models, various heritabilities, and various sample-selection threshold values) by simulation studies. The results indicated that the statistic TpE is robust and powerful for genomic association study of QTL. A simulation study based on the haplotype frequencies of 10 single nucleotide polymorphisms (SNPs) of angiotensin-I converting enzyme genes was conducted to evaluate the performance of the statistic TPE for genetic association study.
基金supported by the National Natural Science Foundation of China (No.60525303, 60604004, 60704009) Natural Science Foundationof Hebei Province, China (No.F2005000390, F2006000270)
文摘This paper considers the problem of delay-dependent exponential stability in mean square for stochastic systems with polytopic-type uncertainties and time-varying delay. Applying the descriptor model transformation and introducing free weighting matrices, a new type of Lyapunov-Krasovskii functional is constructed based on linear matrix inequalities (LMIs), and some new delay-dependent criteria are obtained. These criteria include the delay-independent/rate- dependent and delay-dependent/rate-independent exponential stability criteria. These new criteria are less conservative than existing ones. Numerical examples demonstrate that these new criteria are effective and are an improvement over existing ones.
基金This study was funded by Science and Technology Department of Zhejiang Province(2014C33126)Public Welfare Technology Research and Social Development Projects(2014-)+3 种基金This study is also funded by the National Natural Science Foundation of China(81201057)Shanghai Municipal Health Bureau Project(20124109)Chinese Medical Association,Psychiatry-Servier Youth Research Fund,Shanghai Mental Health Center international cooperation project(2013-)Shanghai Municipal Center for Mental Health Clinical Research Program.This study is funded by Key Research Project of Zhejiang TCM Science and Technology Plan of China(2018ZZ010).
文摘Background The prevalence of Alzheimer's disease(AD)comorbid with depression is common.However,the mechanisms of AD with depression remain unclear.Aims To investigate the regional alterations of brain activity of AD with depression in resting-state functional magnetic resonance imaging(rs-fMRI).Methods 154 patients with AD who met the inclusion criteria were recruited from the Zhejiang Provincial People’s Hospital from October 2014 to October 2016.According to whether the core symptoms of depression were present,patients were divided into two groups,22 patients with AD with depression(AD-D)and 52 patients with AD without depression(AD-nD).The amplitude of low frequency fluctuations(ALFF)was compared between two groups by performing independent-samples f-test.Results Compared with the AD-D group,increased ALFF values in the bilateral superior frontal gyrus,left middle frontal gyrus and left inferior frontal gyrus were observed in the AD-nD group.The brain activity in the AD-nD group in the bilateral superior frontal gyrus,left middle frontal gyrus and the left inferior frontal gyrus was higher than the AD-D group.Conclusions Resting-state brain functional alterations may be closely bound up with the pathophysiologic features of patients with AD with depressive symptoms.
基金This work was supported by the National Natural Science Foundation of China(No.60474023)Science and Technology Key Project Fund of theMinistry of Education(No.03184)the Major State Basic Research Development Program of China(No.2002CB312200).
文摘A precedence order is defined based on the release dates of jobs' direct successors. Using the defined precedence order and Heap Sort, a new polynomial algorithm is provided which aims to solve the parallel scheduling problem P|pj = 1, r j, outtree| ∑Cj Cj. The new algorithm is shown to be more compact and easier to implement.
基金supported by the National Key Research and Development Project of China (2022YFD1601102)the Key R&D Plan of Heilongjiang Province, China (JD22B002)+1 种基金the Program on Industrial Technology System of National Soybean, China (CARS-04-PS17)the UNDP Project, China (cpr/21/401) and the National Natural Science Foundation of China (41771284)
文摘Straw mulching is a widespread practice for reducing the soil carbon loss caused by erosion.However,the effects of straw mulching on dissolved organic matter(DOM)runoff loss from black soil are not well studied.How straw mulching affects the composition and loss of runoff DOM by changing soil aggregates remains largely unclear.Here,a straw mulching treatment was compared to a no mulching treatment(as a control)on sloping farmland with black soil erosion in Northeast China.We divided the soil into large macroaggregates(>2 mm),small macroaggregates(0.25-2 mm),and microaggregates(<0.25 mm).After five rain events,the effects of straw mulching on the concentration(characterized by dissolved organic carbon(DoC)and composition(analyzed by fluorescence spectroscopy)of runoff and soil aggregate DOM were studied.The results showed that straw mulching reduced the runoff amount by 54.7%.Therefore,although straw mulching increased the average DOc concentration in runoff,it reduced the total runoff DOM loss by 48.3%.The composition of runoff DOM is similar to that of soil,as both contain humic-like acid and protein-like components.With straw mulching treatment,the protein-like components in small macroaggregates accumulated and the protein-like components in runoff declined with rain events.Fluorescence spectroscopy technology may help in understanding the hydrological paths of rain events by capturing the dynamic changes of runoff and soil DOM characteristics.A variation partitioning analysis(VPA)indicated that the DOM concentration and composition of microaggregates explained 68.2%of the change in runoff DOM from no mulching plots,while the change in runoff DOM from straw mulching plots was dominated by small macroaggregates at a rate of 55.1%.Taken together,our results demonstrated that straw mulching reduces the fragmentation of small macroaggregates and the loss of microaggregates,thus effecting DOM compositions in soil and reducing the DOM loss in runoff.These results provide a theoretical basis for reducing carbon loss in sloping farmland.
基金Supported by the National Natural Science Foundation of China(No.42077335)。
文摘To effectively remove n-propylbenzene(n-PBZ)and isopropylbenzene(i-PBZ)leaked into seawater using Rhinomonas reticulata S6A(a newly isolated marine microalga),the effects of three inorganic nutrients and four environmental factors on their degradation were determined after 7 d of inoculation.Results show that NaNO_(3) at 300 mg/L caused a higher removal efficiency of both n-PBZ and i-PBZ(44.79%and 39.26%),while for NaH_(2) PO_(4)·H_(2) O,greater removal rates of two PBZs(47.30%and 42.23%)were achieved at 30 and 20 mg/L,respectively.NaHCO_(3) supplementation(500-750 mg/L)resulted in a large reduction(43.67%-45.04%)in i-PBZ concentration.The change in seawater pH(from 6 to 9)did not affect the elimination of n-PBZ and i-PBZ.The most suitable salinity and temperature were 30 and 25-30℃,respectively,leading to the PBZs removal of~40%.Light intensity exhibited significant influence on elimination of PBZs,and the maximum removal efficiencies of 56.07%(n-PBZ)and 55.00%(i-PBZ)were recorded under 200 and 600μmol/(m^(2)·s),respectively.In addition,the microalga could still remove PBZs when it failed to grow well due to darkness,strong light,low temperature,or low salinity,which might mean that good growth of alga is not always a necessary condition for PBZs removal.Therefore,attention should be paid to the suitability of nutrient levels and environmental conditions(excluding pH)in seawater when using microalgae for bioremediating PBZs-contaminated seawater.
基金the National Natural Science Foundation of China(Grant No.:82360542)Jiangxi Provincial Natural Science Foundation,China(Grant Nos.:20224BAB214030 and 20224BAB216072)+2 种基金Doctoral Startup Fund of Gannan Medical University,China(Grant Nos.:QD202136 and QD202132)Science and Technology Planning Projects of Fuzhou,China(Grant No.:2021FZR0101)the Natural Science Foundation of Fujian Province,China(Grant No.:2022YZ0104).
文摘Ribosomopathies encompass a spectrum of disorders arising from impaired ribosome biogenesis and reduced functionality.Mutation or dysexpression of the genes that disturb any finely regulated steps of ribosome biogenesis can result in different types of ribosomopathies in clinic,collectively known as ribosomopathy genes.Emerging data suggest that ribosomopathy patients exhibit a significantly heightened susceptibility to cancer.Abnormal ribosome biogenesis and dysregulation of some ribosomopathy genes have also been found to be intimately associated with cancer development.The correlation between ribosome biogenesis or ribosomopathy and the development of malignancies has been well established.This work aims to review the recent advances in the research of ribosomopathy genes among human cancers and meanwhile,to excavate the potential role of these genes,which have not or rarely been reported in cancer,in the disease development across cancers.We plan to establish a theoretical framework between the ribosomopathy gene and cancer development,to further facilitate the potential of these genes as diagnostic biomarker as well as pharmaceutical targets for cancer treatment.
基金the National Natural Science Foundation of China(Grant No.31601756)the National Science Fund of Yunnan for Distinguished Young Scholars(Grant No.202001AV070003)。
文摘The MYB transcription factor is one of the largest gene families in plants,playing an important role in regulating plant growth,development,response to stress,senescence,and especially the anthocyanin biosynthesis.In this study,A total of 217 MYB genes,including 901R-MYBs,124 R2R3-MYBs,and 3 R1R2R3-MYBs have been identified from the potato genome.The 1R-MYB and R2R3-MYB family members could be divided into 20 and 35 subgroups respectively.Analysis of gene structure and protein motifs revealed that members within the same subgroup presented similar exon/intron and motif organization,further supporting the results of phylogenetic analysis.Potato is an ideal plant to reveal the tissue-specific anthocyanins biosynthesis regulated by MYB,as the anthocyanins could be accumulated in different tissues,showing colorful phenotypes.Five pairs of colored and colorless tissues,stigma,petal,stem,leaf,and tuber flesh,were applied to the transcriptomic analysis.A total of 70 MYB genes were found to be differentially expressed between colored and colorless tissues,and these differentially expressed genes were suspected to regulate the biosynthesis of anthocyanin of different tissues.Co-expression analysis identified numerous potential interactive regulators of anthocyanins biosynthesis,involving 39 MYBs,24 bHLHs,2 WD-repeats,and 29 biosynthesis genes.Genome-wide association study(GWAS)of tuber flesh color revealed amajor signal at the end of Chromosome 10,which was co-localized with reported I gene(StMYB88),controlling tuber peel color.Analyses of DEGs(Differentially Expression Genes)revealed that both StMYB88 and StMYB89 were closely related to regulating anthocyanin biosynthesis of tuber flesh.This work offers a comprehensive overview of the MYB family in potato and will lay a foundation for the functional validation of these genes in the tissue-specific regulation of anthocyanin biosynthesis.
基金supported by grants from the Ministry of Science and Technology of China (Grant No.2015CB931804)National Natural Science Foundation of China (Grant No.U1505225, 81773063, 81273548, 81571802)+1 种基金the Natural Science Foundation of Fujian Province (Grant No.2016J06020)Fujian Development and Reform Commission project (Grant No.829054)
文摘Objective: Cancer metastasis remains the primary cause of cancer-related death worldwide.In a previous study, we found that levels of BTB/POZ domain-containing protein KCTD12 are lower in metastatic melanoma cells than in parental melanoma cells.The purpose of this study was to identify the roles of KCTD12 in cancer metastasis.Methods: The Cancer Genome Atlas(TCGA) datasets were used to evaluate the relationship between KCTD12 and skin cutaneous melanoma(SKCM) prognosis.The effects of endogenous KCTD12 on biological behaviors were examined using the MTT assay.The impacts of KCTD12 on melanoma stemness were explored using spheroid formation assay.KCTD12 knockout A375 cells were generated to confirm the inhibitory effect of KCTD12 on CD271, and a mouse metastatic model was used to determine the impact of KCTD12 on melanoma metastasis in vivo.Results: KCTD12 levels were lower in lung metastatic cells than in paired parental melanoma cells, and low KCTD12 expression indicated a poor prognosis in SKCM.Cancer metastasis-related capacities were higher in lung metastatic cells than in parental melanoma cells.Moreover, KCTD12 knockdown enhanced tumor growth and metastasis both in vitro and in vivo.Mechanistically, the interaction between KCTD12 and CD271 might be responsible for the stemness transformation after KCTD12 knockdown.Conclusions: This study identifies for the first time the role of the interaction between KCTD12 and CD271 in inducing melanoma cell stemness transformation.Moreover, KCTD12 repression enhances melanoma cell growth, adhesion, migration and invasion.
文摘This present study was aimed to investigate the roles of the receptors of Thl/Th2 cytokines and chemokines in lhe pathogenesis of chronic idiopathic urticaria (CIU). Thirty patients with CIU, 30 patients with dermographism and 30 healthy controls were randomly enrolled. Reverse transcription-PCR (RT-PCR) was used to analyze the mRNA of cytokine receptors in peripheral blood mononuclear cells (PBMCs). The mRNA levels of tumor necro- sis factor receptor (TNFR), interferon-7 receptor (IFN-yR), and interleukin-10 receptor (IL-IOR) were statistically increased in the CIU group (P 〈 0.05), while IL-2R, IL-4R, IL-6R, and IL-13R showed no significant differences between the CIU and other groups. The mRNA levels of CCR3 and CCR6 were statistically increased in the CIU group (P 〈 0.05). The toll-like receptor 2 (TLR2) mRNA level was significantly lower in the CIU group than the healthy control group (P 〈 0.05). These findings indicate that the regulation of mRNA of TNFR, IFN-γR, IL-IOR, CCR3, CCR6 and TLR2 may be involved in the pathogenesis of CIU.
文摘Objective:To understand the rate of cognition and protection of COVID-19 in a community in Hubei province.Methods:The related knowledge,epidemic situation and self-protective measures of COVID-19 were investigated by mobile phone questionnaire among 836 people in a community in Hubei province.Results:The rate of the awareness of COVID-19’s relevant knowledge and the ways to obtain the epidemic situation varied among different age groups.After multi-channel intervention in the community,the rate of the awareness of COVID-19’s relevant knowledge increased from 75.0%to 96.2%,and the implementation rate of personal protection increased from 59.6%to 92.6%.Conclusion:It is of great significance to improve residents’awareness and protection consciousness and block the spread of COVID-19 in the community through multi-channel intervention.
基金supported by the National Natural Science Foundation of China(32225033,32071496)Ministry of Science and Technology of China(2022YFF1301500)。
文摘Climate and land-use changes are predicted to impact biodiversity,threatening ecosystem services and functions.However,their combined effects on the functional diversity of mammals at the regional scale remain unclear,especially at the beta level.Here,we use projected climate and land-use changes in China to investigate their potential effects on the alpha and beta functional diversities of terrestrial mammals under low-and high-emission scenarios.In the current projection,we showed strong positive spatial correlations between functional richness and species richness.Functional evenness(FEve),functional specialization(FSpe),and functional originality(FOri)decreased with species richness,and functional divergence(FDiv)increased first and then plateaued.Functional beta diversity was dominated by its nestedness component,in contrast to the taxonomic facet.Potential changes in species richness are more strongly influenced by land-use change under the low-emission scenario,while under the high-emission scenario,they are more strongly influenced by climate change.Changes in functional richness(FRic)were inconsistent with those in species richness,with a magnitude of decreases greater than predicted from species richness.Moreover,mammal assemblages showed potential functional differentiation(FD)across the country,and the trends exceeded those towards taxonomic differentiation(TD).Our findings help us understand the processes underlying biodiversity responses to global changes on multiple facets and provide new insight for conservation plans.
基金funded by the National 13th Five-Year Plan for Major New Drug Development of China(No.2017ZX09302010)
文摘Background:Atopic dermatitis(AD)affects approximately 10%of adults worldwide.CM310 is a humanized monoclonal antibody targeting interleukin-4 receptor alpha that blocks interleukin-4 and interleukin-13 signaling.This trial aimed to evaluate the efficacy and safety of CM310 in Chinese adults with moderate-to-severe AD.Methods:This multicenter,randomized,double-blind,placebo-controlled,phase 2b trial was conducted in 21 medical institutions in China from February to November 2021.Totally 120 eligible patients were enrolled and randomized(1:1:1)to receive subcutaneous injections of 300 mg CM310,150 mg CM310,or placebo every 2 weeks for 16 weeks,followed by an 8-week follow-up period.The primary endpoint was the proportion of patients achieving≥75%improvement in the Eczema Area and Severity Index(EASI-75)score from baseline at week 16.Safety and pharmacodynamics were also studied.Results:At week 16,the proportion of EASI-75 responders from baseline was significantly higher in the CM310 groups(70%[28/40]for high-dose and 65%[26/40]for low-dose)than that in the placebo group(20%[8/40]).The differences in EASI-75 response rate were 50%(high vs.placebo,95%CI 31%-69%)and 45%(low vs.placebo,95%CI 26%-64%),with both P values<0.0001.CM310 at both doses also significantly improved the EASI score,Investigator’s Global Assessment score,daily peak pruritus Numerical Rating Scale,AD-affected body surface area,and Dermatology Life Quality Index compared with placebo.CM310 treatment reduced levels of thymus and activation-regulated chemokine,total immunoglobulin E,lactate dehydrogenase,and blood eosinophils.The incidence of treatment-emergent adverse events(TEAEs)was similar among all three groups,with the most common TEAEs reported being upper respiratory tract infection,atopic dermatitis,hyperlipidemia,and hyperuricemia.No severe adverse events were deemed to be attributed to CM310.Conclusion:CM310 at 150 mg and 300 mg every 2 weeks demonstrated significant efficacy and was well-tolerated in adults with moderate-to-severe AD.Trial Registration:ClinicalTrials.gov,NCT04805411.
文摘This study examined how a dialogic approach in an online media literacy class at a university in China helped to develop college students’global awareness when the world was disrupted by the coronavirus disease(COVID-19).Using writing exemplars from students’online dialogues and reflective journals,this article demonstrates the potentialities of an online dialogic approach to guide a sense of togetherness and critical solidarity.The digital dialogical approach provides an expanded space for students to converse with multiple voices,meditate on tensions,and rethink their own stances as citizens of their country and the world.The article also underscores the role of higher education in cultivating a sense of global community among the younger generation and bridging the ideological divide in society.
基金Project supported by Natural Science Foundation of Inner Mongolia Autonomous Region of China(2019LH02006,2021LHMS04003)。
文摘ABO_(3)-type perovskite oxides with abounding defect structures and diverse physical chemistry attributes have been extensively studied in heterogeneous catalysis.Semiconductor LaFeO_(3)perovskites fabricated via the sol-gel method were used as peroxydisulfate(PDS)activators for methylene blue(MB)degradation under low-intensity ultra-violet(UV)-light to evaluate the degradation efficiency of organic pollutants and mechanism.The results indicate that under low-intensity UV irradiation,the developed UV/LaFeO_(3)/PDS system shows excellent degradation ability of organic pollutants in a wide pH range.Electron spin resonance and radical quenching experiments verify that SO_(4)^(·-),·OH,h^(+)and ^(1)O_(2)are generated during the activation process,and ^(1)O_(2)plays a dominant role in MB degradation.Reduction of low oxidation state Fe(Ⅱ)and O_(2)^(2-)/O^(-)on the LaFeO_(3)surface shows that oxygen vacancy,as the electron transfer mediator,enhances the redox cycle efficiency of Fe(Ⅱ)/Fe(Ⅲ).Photogenerated electrons of LaFeO_(3)involved in the cyclic transformation of Fe(Ⅱ)/Fe(Ⅲ)and PDS activation further increase the number of active radicals and thus promote the synergistic effect of photocatalytic coupled with sulfate radicalbased advanced oxidation processes.
基金This work was supported by the grants from the National Natural Science Foundation of China(81961138017,81773063,and U1505225)Ministry of Science and Technology of China(2015CB931804)Young and Middle-aged Teacher Education Research Project of Fujian Province,JAT190623。
文摘Dear Editor,Metastasis is the cause of most fatalities in cancer patients and remains the phenomenon poorly understood mechanistically. Deciphering of the regulatory networks underlying the cancer cell metastasis is urgently needed. Nucleolar protein 7 (NOL7) has been reported to function as a tumor suppressor in cervical cancer.1 Our current study reveals a novel tumor-promoting capacity of NOL7 in melanoma. We first detected that NOL7 expression is upregulated in metastatic melanoma as compared with its expression at the primary site through isobaric tag for relative and absolute quantitation proteomic screening, further confirming this finding with the analysis of NOL7 protein and messenger RNA (mRNA) levels (Supplementary Fig. S1). Importantly, NOL7 expression increased with the disease progression from benign nevus to primary melanoma and further to metastatic melanoma (Fig. 1a and Supplementary Fig. S1d). Previous studies have shown that melanoma is commonly associated with the amplification of the chromosome region 6p, particularly 6p21–23, where the NOL7 gene resides, and that this region frequently undergoes heterozygous loss in cervical cancer.2 It might therefore be predicted that NOL7 exhibits a different expression pattern and plays different roles in melanoma and cervical cancer.