Transcription factor CabHLH035 promotes cold resistance and homeostasis of reactive oxygen species in pepper

Plant basic helix-loop-helix(bHLH)transcription factors(TFs)play central roles in various abiotic stresses.However,its role in plant cold resistance is largely unknown.Previously,we characterised CaNAC035 in pepper,which positively regulates tolerance to cold,salt and drought stresses tolerance.Here,we identified CabHLH035,a CaNAC035-interacting protein in pepper.To explore its functions in cold stress tolerance,we silenced the gene in pepper via virus-induced gene silencing(VIGS)and overexpressed the gene in Arabidopsis.The results showed that CabHLH035 expression was induced by cold treatment,and silencing of CabHLH035 decreased cold stress tolerance.Conversely,overexpression of CabHLH035 in Arabidopsis increased cold stress tolerance.To investigate homologs genes of C-repeat binding factor(CBF)pathway proteins and reactive oxygen species(ROS)marker gene expression blocking by CabHLH035,we performed yeast one-hybrid(Y1H),dual luciferase and electrophoretic mobility shift assay experiments.The results showed that CabHLH035 bound to the region upstream of the CaCBF1A and CaAPX promoters.Additionally,CaCBF1A bound to the CaDHN4 promoter.Taken together,our results showed that CabHLH035 plays a crucial role in cold stress tolerance and its potential as a target for breeding cold-resistant crops.The findings provide a basis for studying the functions and regulatory network of cold stress tolerance in pepper.

Polysaccharide extract from Rosa laevigata fruit attenuates inflammatory obesity by targeting redox balance and gut interface in high-fat diet-fed rats

Low-molecular-weight polysaccharides(RLPs)extracted from Rosa laevigata fruits showed multiple biofunctions in Asia.This study aimed to investigate its anti-inflammatory obesity effect in high-fat dietfed rats and further elucidated the underlying molecular mechanism by multi-omics methods.The results showed that RLPs administration had significantly restored immune organ indexes and reduced body weight gain.RNA-seq revealed that the effect of RLPs was partially attributed to its regulation on PPARs signaling by increasing the expressions of Scd,Acox3 and Hmgcs2,and on other redox-related pathways by decreasing the expressions of Cyp2 e1,Il1-r1 and Lbp.Moreover,16 S rRNA sequencing coupled with metagenome sequencing showed that RLPs had significantly reduced the ratio of Firmicutes/Bacteroidetes from 8.01 to2.37,and significantly increased the relative abundances of Alistipes,Prevotella,and Akkermansia from 0.36%,1.10%and 2.61%to 0.65%,2.37%and 4.42%,respectively.Spearman correlation analysis result indicated that the abundances of Lachnospiraceae,Prevotella and Bacteroidales were significantly negatively correlated with obesity phenotype,liver function and inflammatory factors.These results revealed that RLPs exerted significant anti-inflammatory obesity property partially via regulation on gut microbiota interface and the redox balance.Therefore,RLPs could be a promising functional food resource with the potential for redox imb alance-related diseases chemoprevention.

Research Progress on the Invasion Mechanism of Erigeron annuus

Erigeron annuus(L.)Pers.,an alien species,is widely distributed in most areas of China.It was included in the third batch of invasive alien species list by the Ministry of Ecology and Environment of China,and it is a first-level malignant invasive plant.Due to its great reproducibility with a mass of seeds,power adaptability to the external environment and strong allelopathy,it has highly invasive and adaptability,and has affected the development of the agriculture and forestry,the diversity of local species and the ecosystem.In this paper,we summarized the current invasion situation,invasion and adaptation mechanism of E.annuus,and prospected further research on E.annuus invasion mechanism,hoping to provide a reference for realizing its effective management.

Optimization of Enzymatic Extraction of Sodium Chondroitin Sulfate From Bovine Nasal Bone

Taking bovine nasal bone as a raw material,the sodium chondroitin sulfate was extracted by enzymolysis-ion adsorption-alcohol precipitation method,and the enzymolysis process was optimized.After single factor experiments,the response surface analysis was performed by software Design-Expert 8.0,and the optimal enzymolysis conditions were as follows:the trypsin concentration of 0.8%,the pH value of 9.0,the enzymolysis temperature at 52℃,and the enzymolysis time of 2.0 h.With this combination of conditions,the concentration of sodium chondroitin sulfate extract reached 11.3 g/L,the bovine nasal bone yield was 35%,and the finished product content was 92.5%,which provided guidance for industrial production.

Joint Toxicity Determination of Clopyralid,Picloram and Benazolin Mixtures on Broad-leaved Weed Lapsana apogonoides Maxim.in Rape Fields

[Objectives]This study was conducted to investigate the compounding of three herbicides:clopyralid,picloram and benazolin,so as to improve the effectiveness of herbicides.[Methods]With Lapsana apogonoides Maxim.as the target and clopyralid,picloram and benazolin as the test agents,seven gradient concentrations were set up to determine the joint toxicity of the three agents.[Results]When the compounding ratio of picloram,clopyralid and benazolin was 2∶1∶6,the maximum co-toxicity coefficient was 290.0.[Conclusions]The compounding of picloram,clopyralid and benazolin has a significant synergistic effect on L.apogonoides,which reduces production costs and environmental pressure,providing technical support for the effective control of broad-leaved weeds such as L.apogonoides.

Allelopathic Effects of Extracts from Three Asteraceae Plants on Seed Germination and Seedling Growth of Four Plants

[Objectives]This study was conducted to investigate the allelopathic effects of the extracts of Asteraceae plants on plant seeds and seedlings.[Methods]The effects of the extracts of three Asteraceae plants(Erigeron annuus,Bidens pilosa and Inula japonica)on the seed germination and seedling growth of four plants(Setaria viridis,Erigeron canadensis,Chenopodium album and Lactuca sativa)were studied by the Petri dish filter paper method and bioassay method.[Results]Different concentrations(10,20,40,100,200 mg/ml)of the extracts of E.annuus,B.pilosa and I.japonica had different allelopathic effects on the seed germination of the same recipient plant,and with the increase of the concentrations of the extracts,the inhibitory effects were more significant.The extract of B.pilosa had the strongest inhibitory effect.The extracts at the low concentration(10 mg/ml)had no significant effects on the germination rates and germination energy of the four kinds of plant seeds.In terms of root growth and biomass,the extracts of E.annuus and I.japonica showed the effect of low promotion and high inhibition.Under the treatment with high concentrations(100,200 mg/ml)of the extracts from the three Asteraceae plants,the germination of plant seeds,plant root length,stem height and biomass were inhibited.With the concentrations of the extracts of the three Compositae plants increasing,the chlorophyll contents of the four plants showed a downward trend,and the activity of SOD,POD,CAT and other antioxidant protective enzymes showed a trend of first increasing and then decreasing.[Conclusions]These three species of Asteraceae plants had certain allelopathic inhibitory effects on the germination and growth of the four kinds of plant seeds,including S.viridis,E.canadensis,C.album and L.sativa,and they have potential in the development of botanical herbicides or plant protection.

Study on the Resistance Levels of Different Echinochloa crusgalli Populations to Penoxsulam and Bispyribac-sodium

[Objectives]This study was conducted to screen the resistance of Echinochloa crusgalli in some paddy fields in Changde City,Yiyang City and Yueyang City in Hunan Province to penoxsulam and bispyribac-sodium.[Methods]The resistance to penoxsulam and bispyribac-sodium was screened in 11 E.crusgalli populations collected from rice production areas in Changde,Yiyang and Yueyang Cities,Hunan Province,and the resistance level of population HU-2 to penoxsulam was determined.[Results]HU-1 to HU-6 and HU-8 A,a total of seven populations showed high levels of resistance to penoxsulam and bispyribac-sodium.The resistance level of population HU-2 to penoxsulam was extremely high;population HU-7 showed low-level resistance to both drugs;HU-11 population showed low-level resistance to penoxsulam and was susceptible to bispyribac-sodium;and populations HU-8 and HU-9 were susceptible to penoxsulam and bispyribac-sodium.[Conclusions]This study provides a technical basis for the rational use of penoxsulam and bispyribac-sodium and the selection of control chemicals for E.crusgalli in paddy fields.

Effects of Polygonatum odoratum Polysaccharides on the Quality of White Chili Peppers in Jars

[Objectives]This study was conducted to investigate the effects of Polygonatum odoratum polysaccharides on the quality of white chili peppers in jars.[Methods]White chili peppers were pickled by adding different concentrations of P.odoratum polysaccharides with traditional pickling technology,and its nitrite content,pH and sensory quality were analyzed and detected.[Results]The results showed that,compared with the control group without adding P.odoratum polysaccharides,the nitrite content in white chili peppers in jars decreased after adding P.odoratum polysaccharides.When the addition amount of P.odoratum polysaccharides was 0.002%,the inhibition rate of nitrite reached the maximum.Under the same fermentation time,P.odoratum polysaccharides could significantly reduce the pH value of pickles.When the addition amount of P.odoratum polysaccharide was 0.002%and the pickling time was 3 months,the sensory quality of white chili peppers in jars was the best.At this point,white chili peppers in jars had rich aroma and tasted soft and sour,and the sour and salinity were suitable.As the fermentation time continued to increase,the chili aroma of white chili peppers in jars gradually decreased,and the sour taste became more pronounced.[Conclusions]This study can provide reference for the safety and quality control of white chili peppers in jars.

Occurrence and Control of Weeds in Abandoned Fields in Central Hunan

[Objectives]This study was conducted to clarify the occurrence of weeds,and give relevant comprehensive control suggestions accordingly.[Methods]The species and community characteristics of weeds in abandoned rice fields in central Hunan were investigated and analyzed.[Results]There were 75 species of weeds in the abandoned farmland in central Hunan,belonging to 66 genera,27 families.Among them,22 species had a relative abundance of more than 5%.According to the different water environments of abandoned fields,there were differences in the types and structures of weeds.They succeeded to wetland vegetation types and dryland vegetation types respectively.Species grown in the original farmland still exist in abandoned fields,but they were mainly invaded by field weeds,such as Digitaria sanguinalis,Eleusine indica,Panicum acroanthum,Bidens pilosa,and Xanthium sibiricum.[Conclusions]This study lays a foundation for fully guaranteeing the actual results of re-cultivation of abandoned land in rural areas.

Study on the Optimization of Extraction Technology of Rose laevigate Michx. Polysaccharides and Its in vitro Antioxidant Activity

[Objectives]This study was conducted to optimize the optimal process conditions for extracting polysaccharides from Rose laevigate Michx.with hot water.[Methods]The extraction rate of R.laevigate was used as an evaluation index,and extraction temperature,material-to-liquid ratio,extraction time and extraction times were selected as influencing factors.On the basis of single factor tests,L 9(34)orthogonal tests were carried out for optimization,and the content of polysaccharides was determined by the phenol-sulfuric acid method.The in vitro antioxidant activity of the extracted R.laevigate polysaccharides was studied.[Results]The effects of various influencing factors on the extraction rate of R.laevigate polysaccharides ranked as material-liquid ratio>extraction temperature>extraction times>extraction time.The optimal extraction process was extraction temperature 80℃,extraction time 2 h,and material-to-liquid ratio 1∶25,extraction times 3 times,under which the extraction rate was(9.55±0.12)%.R.laevigate polysaccharides had certain scavenging capacities of DPPH free radicals and hydroxyl free radicals,and its scavenging capacity of DPPH free radicals was better than that of hydroxyl free radicals.[Conclusions]This study provides a theoretical basis for the optimization of the extraction process of R.laevigate polysaccharides and the in-depth development and application of polysaccharides.

Study on Dormancy Mechanism and Dormancy-breaking Method of Velvetleaf(Abutilon theophrasti)Seeds

[Objectives]This study was conducted to explore how can we better break the dormancy of velvetleaf seeds and the best method to promote seed germination.[Methods]With the seeds of velvetleaf(Abutilon theophrasti)as experimental materials,the dormancy of velvetleaf seeds was revealed through seed vigor determination,water absorption rate determination and germination tests,and the seeds of velvetleaf were treated by physical and chemical methods to explore the best method for breaking dormancy of velvetleaf seeds.[Results]Poor water permeability of seed coat and endogenous inhibitory substances present in the seeds were the main reasons leading to the dormancy of velvetleaf seeds.The shell-breaking treatment,98%concentrated sulfuric acid treatment and 40%NaOH strong alkali treatment all could break the dormancy obstacle of velvetleaf seed coat.Compositing the above measures with 200 mg/ml gibberellin solution could further improve the germination ability of seeds.Among them,compositing the mechanical shell-breaking treatment with gibberellin had the best effect,followed by the composite of 10 and 15 min of 98%concentrated sulfuric acid treatment with gibberellin.[Conclusions]This study lays a foundation for the utilization and development and the comprehensive prevention and control research of velvetleaf seeds.

Research Progress on Biological Characteristics and Integrated Management of Digitaria sanguinalis

Digitaria sanguinalis is an annual worst weed in the grass family that occurs in all parts of China.It mainly does harm to dryland crops and direct-sowing rice fields.In agricultural production,chemical herbicides are mainly used to control them,but the problems of environmental pollution and drug resistance caused by chemical herbicides have become more and more severe,which is in urgent need of integrated management.In this paper,the biological characteristics,chemical control,microbial control and allelopathy of D.sanguinalis were reviewed.

Research Progress of Application of Microbial Inoculants in Agricultural Production

Microbial inoculants have received increasing attention in strengthening plant biological barriers,antagonizing and inhibiting harmful microorganisms,and ensuring the safe production of agricultural products.This paper summarized the research status of agricultural microbial inoculants,the application of microbial inoculants in agriculture,and the trends and prospects of agricultural microbial research.

基于红外相机技术的林麝行为PAE编码与多样性

了解野生动物在自然环境中的行为表现,对于制定该物种的保护政策尤为重要。野生林麝(Moschus berezovskii)是湖南省分布的几种国家一级重点保护动物之一,但对其在野外的行为表现研究甚为匮乏。2019年4月至2023年6月,作者对湖南壶瓶山国家级自然保护区的固定红外相机监测数据进行了分类汇总,使用PAE编码系统开展林麝的行为谱及多样性研究。本文旨在:(1)参照PAE动物行为编码范式,建立林麝的行为谱及编码系统。(2)对林麝的行为数据进行统计并计算行为多样性指数,分析不同季节、性别、年龄组和生境间的差异。结果显示:(1)通过野生林麝845张有效照片和133段视频辨识了林麝7种姿势、65种动作和32种行为,基本涵盖了林麝的主要日常行为;(2)不同季节的行为多样性指数存在差异,春季(H_(春)=0.8922,r_(春)=0.3850)>夏季(H_(夏)=0.7954,r_(夏)=0.3432)>秋季(H_(秋)=0.7775,r_(秋)=0.3355)>冬季(H_(冬)=0.5094,r_(冬)=0.2198);(3)雄麝、雌麝和幼体行为差异明显,幼体未记录到排遗、标记、自我指向3种行为,雌麝有较多的自我指向行为,雄麝独有标记行为,行为多样性指数表现为雌麝(H_(雌)=0.6338)>雄麝(H_(雄)=0.5814)>幼麝(H_(幼)=0.3816);(4)林麝在针阔混交林的行为多样性指数最高,摄食行为频次居于各生境前列。综上,本研究首次提供了野生林麝的行为谱及其编码,将为深入地开展林麝行为生态学研究和保护管理提供基础数据。

Synthesis and Bioactivities Evaluation of Novel Anthranilic Diamides Containing N-(tert-Butyl)benzohydrazide Moiety as Potent Ryanodine Receptor Activator

Anthranilic diamides have been proven to be a class of efficacious and safe insecticides targeting ryanodine receptors (RyRs) during the last decade. In order to develop innovative scaffolds with high insecticidal activity, two series of N-pyridylpyrazole derivatives containing N-(tert-butyl)benzohydrazide substructures were designed and synthesized. Their insecticidal activities against oriental armyworm (Mythimna separata) and diamondback moth (Plutella xylostella) were evaluated. Preliminary bioassay results revealed that some of the new compounds exhibited good insecticidal activity against the oriental armyworm and diamondback moth. The mode of action of these compounds were verified using calcium-imaging technique and the results showed that some new compounds could effectively modulate the insect cytosolic Ca^2+ level, and break the cellular calcium homeostasis, indicating some of these compounds were potent activators of the RyRs.

The unusual physicochemical properties of azulene and azulene-based compounds

Azulene,an isomer of naphthalene,has become one of hot chemical structures in the research field of functional materials,due to its anti-Kasha's rule emissions and unusual physicochemical properties(e.g.,photophysical,electrochemical,and photoelectrochemical properties).In the past,the synthesis of azulene-based compounds is relatively inconvenient.Recently,there have been more and more reports about the synthesis strategies of the azulene-based compounds for finely tuning the physicochemical properties.In this article,we introduce several synthetic methods for kinds of azulene-based compounds which has unusual physicochemical properties.With these convenient methods and unique physicochemical properties,azulene-based compounds can be applied into many fields such as specific bioimaging,advanced molecular switches,organic field-effect transistor(OFET),organic light emitting diode(OLED),solar cells,and so forth.And these properties are also summarized here.

Design, synthesis and SAR study of novel sulfonylurea derivatives containing arylpyrimidine moieties as potential anti-phytopathogenic fungal agents

Acetohydroxyacid synthase(AHAS) was considered as a promising target for antifungal agents.Herein,three series of novel sulfonylureas(SUs) 9-11 containing aromatic-substituted pyrimidines were designed and synthesized according to pharmacophore-combination and bioisosterism strategy.The in vitro fungicidal activities against ten phytopathogenic fungi indicated that most of the title compounds exhibited broad-spectrum and excellent fungicidal activities.Based on the preliminary fungicidal activities,a CoMFA model was constructed and the 3 D-QSAR analysis indicated that either a bulky group around the 5-position of the pyrimidine ring or electropositive group around the 2-position of the benzene ring would be favour to fungicidal activities.In order to study interaction mechanism,10 k was automatically docked into yeast AHAS and it further indicated that bearing bulky groups-aryl at the pyrimidine ring was critical to enhance antifungal activities.It revealed that the antifungal activity of derivatives 9-11 probably results from the inhibition of fungal AHAS.Thus,the present results strongly showed that SUs should be considered as lead compounds or model molecules to develop novel antiphyt o pathogenic fungal agents.

High-contrast flicker luminescence on dynamic covalent structure based nanoaggregates

In nanoscience, molecular switches have played a significant role to deliver different control abilities to practical functions,whereas high-contrast luminescence switchable manipulation at nanoscale is still limited. Since the tuning for emission behavior with high contrast ratio strongly connects to numerous visualized sensing and optoelectronic applications, we here report that autonomous p H control can be enrolled to address a high-contrast molecular emission change at the nanoaggregated level, for gaining a flicker luminescence performance in aqueous media. Employing a BODIPY contained dynamic covalent dye, we find its luminescent signal and nanoaggregate size can be spontaneously adjusted in water. On this basis, high-contrast luminescence switching of the material can be achieved upon the alternate introduction of base and acid into the aggregation state. Such a behavior can be attributed to a p H triggered photo-induced electron transfer regulation process. The dye aggregates can be well endocytosed for bioimaging and its luminescent variation can be autonomously displayed as a flicker effect. These results provide new visions for the design and development of smart materials with a dynamic luminescence behavior.

Transformer-based DNA methylation detection on ionic signals from Oxford Nanopore sequencing data

Background:Oxford Nanopore long-read sequencing technology addresses current limitations for DNA methylation detection that are inherent in short-read bisulfite sequencing or methylation microarrays.A number of analytical tools,such as Nanopolish,Guppy/Tombo and DeepMod,have been developed to detect DNA methylation on Nanopore data.However,additional improvements can be made in computational efficiency,prediction accuracy,and contextual interpretation on complex genomics regions(such as repetitive regions,low GC density regions).Method:In the current study,we apply Transformer architecture to detect DNA methylation on ionic signals from Oxford Nanopore sequencing data.Transformer is an algorithm that adopts self-attention architecture in the neural networks and has been widely used in natural language processing.Results:Compared to traditional deep-learning method such as convolutional neural network(CNN)and recurrent neural network(RNN),Transformer may have specific advantages in DNA methylation detection,because the self-attention mechanism can assist the relationship detection between bases that are far from each other and pay more attention to important bases that carry characteristic methylation-specific signals within a specific sequence context.Conclusion:We demonstrated the ability of Transformers to detect methylation on ionic signal data.