Photodynamic therapy with reactive oxygen species production is a prospective treatment to combat cancer stem cells(CSCs).However,the innate drawbacks,including short lifetime and diffusion distance of reactive oxygen...Photodynamic therapy with reactive oxygen species production is a prospective treatment to combat cancer stem cells(CSCs).However,the innate drawbacks,including short lifetime and diffusion distance of reactive oxygen species and hypoxia within solid tumors,have become bottlenecks for clinical applications of photodynamic therapy.Here,we develop a mitochondria-targeting hemicyanine-oleic acid conjugate(CyOA),which can self-assemble into supramolecular nanoparticles(NPs)without any exogenous excipients.CyOA is also shown for targeting the mitochondrial complex II protein succinate dehydrogenase to inhibit oxidative phosphorylation and reverse tumor hypoxia,resulting in 50.4-fold higher phototoxicity against breast cancer stem cells(BCSCs)compared to SO_(3)-CyOA NPs that cannot target to mitochondria.In 4T1 and BCSC tumor models,CyOA NPs achieve higher tumor inhibition and less lung metastasis nodules compared to the clinically used photosensitizer Hiporfin.This study develops a self-assembled small molecule that can serve as both oxidative phosphorylation inhibitor and photosensitizer for eradication of CSCs and treatment of solid tumors.展开更多
Speckle patterns generated by the intermodal interference of multimode fibers enable accurate broadband wavelength measurements.However,the measurement speed is limited by the frame rate of the camera that captures th...Speckle patterns generated by the intermodal interference of multimode fibers enable accurate broadband wavelength measurements.However,the measurement speed is limited by the frame rate of the camera that captures the patterns.We propose a compact and cost-effective ultrafast wavemeter based on multimode and multicore fibers,which employs spectral-spatial-temporal mapping.The speckle patterns generated by multimode fibers enable spectral-to-spatial mapping,which is then sampled by a multicore fiber into a pulse sequence to implement spatial-to-temporal mapping.A high-speed single-pixel photodetector is employed to capture the pulse sequence,which is analysed using a multilayer perceptron to estimate the wavelength.The feasibility of the proposed wavelength estimation method is experimentally verified,achieving a measurement rate of 100 MHz with a resolution of 2.7 pm in a 1 nm operation bandwidth.展开更多
基金supported by the National Research and Development Program of China(2018YFA0208900,2020YFA0710700,and 2020YFA0211200)the National Science Foundation of China(82172757 and 31972927)+2 种基金the Program for HUST Academic Frontier Youth Team(2018QYTD01)the Scientific Research Foundation of HUST(3004170130)the HCP Program for HUST.
文摘Photodynamic therapy with reactive oxygen species production is a prospective treatment to combat cancer stem cells(CSCs).However,the innate drawbacks,including short lifetime and diffusion distance of reactive oxygen species and hypoxia within solid tumors,have become bottlenecks for clinical applications of photodynamic therapy.Here,we develop a mitochondria-targeting hemicyanine-oleic acid conjugate(CyOA),which can self-assemble into supramolecular nanoparticles(NPs)without any exogenous excipients.CyOA is also shown for targeting the mitochondrial complex II protein succinate dehydrogenase to inhibit oxidative phosphorylation and reverse tumor hypoxia,resulting in 50.4-fold higher phototoxicity against breast cancer stem cells(BCSCs)compared to SO_(3)-CyOA NPs that cannot target to mitochondria.In 4T1 and BCSC tumor models,CyOA NPs achieve higher tumor inhibition and less lung metastasis nodules compared to the clinically used photosensitizer Hiporfin.This study develops a self-assembled small molecule that can serve as both oxidative phosphorylation inhibitor and photosensitizer for eradication of CSCs and treatment of solid tumors.
基金supported by the National Key Research and Development Program of China(2021YFB2800902)National Natural Science Foundation of China(61931010,62225110)Hubei Province Key Research and Development Program(2020BAA006).
文摘Speckle patterns generated by the intermodal interference of multimode fibers enable accurate broadband wavelength measurements.However,the measurement speed is limited by the frame rate of the camera that captures the patterns.We propose a compact and cost-effective ultrafast wavemeter based on multimode and multicore fibers,which employs spectral-spatial-temporal mapping.The speckle patterns generated by multimode fibers enable spectral-to-spatial mapping,which is then sampled by a multicore fiber into a pulse sequence to implement spatial-to-temporal mapping.A high-speed single-pixel photodetector is employed to capture the pulse sequence,which is analysed using a multilayer perceptron to estimate the wavelength.The feasibility of the proposed wavelength estimation method is experimentally verified,achieving a measurement rate of 100 MHz with a resolution of 2.7 pm in a 1 nm operation bandwidth.
