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P物质/NK1受体对实验性胃溃疡大鼠胃肠动力的调节 被引量:2
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作者 王舒怡 徐进 +3 位作者 葛婉悦 王佑华 张玉平 吴靖芳 《神经药理学报》 2021年第6期10-16,共7页
目的:通过检测大鼠实验性胃溃疡愈合过程中胃肠功能及P物质(substance P,SP)表达改变,探讨SP/NK1受体(neurokinin1,NK1)通路对胃溃疡离体幽门平滑肌条收缩功能的影响及机制。方法:62只SD大鼠分为正常组(6只)、盐水对照组(24只)和溃疡组... 目的:通过检测大鼠实验性胃溃疡愈合过程中胃肠功能及P物质(substance P,SP)表达改变,探讨SP/NK1受体(neurokinin1,NK1)通路对胃溃疡离体幽门平滑肌条收缩功能的影响及机制。方法:62只SD大鼠分为正常组(6只)、盐水对照组(24只)和溃疡组(32只)。采用常规冰乙酸腐蚀法制备大鼠乙酸性胃溃疡模型并测量溃疡面积;免疫组化染色观察SP的表达。甲基纤维素酚红溶液灌胃观察胃排空率和肠推进功能;张力换能器及多通道生理信号采集处理系统观察SP及NK1受体拮抗剂对离体胃幽门环形平滑肌条收缩反应性的影响。结果:溃疡组术后4 d形成呈典型的“环堤征”,10~14 d溃疡逐渐愈合,28 d溃疡处形成瘢痕组织。免疫组化结果显示:SP阳性细胞主要分布于黏膜胃底腺细胞、黏膜下层和肌层,溃疡4、10和14 d SP积分光密度值明显低于正常组和对照组(P<0.05,P<0.01)。溃疡组的胃排空率明显低于正常组和盐水组(P<0.05),盐水组胃排空率与正常组相比无统计学意义。幽门环形肌条收缩功能显示:溃疡组不同时相的肌肉收缩幅度与正常组相比均有不同程度降低(P<0.01);加入SP后,胃幽门环形肌收缩幅度增加,在加入NK1受体拮抗剂后,收缩幅度、收缩频率大幅度下降(P<0.05,P<0.01)。结论:胃溃疡时胃肠运动功能显著降低,其机制与SP/NK1受体信号通路受损有关。 展开更多
关键词 胃溃疡 NK1受体 P物质 动力
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Effects of chilling tolerance induced by spermidine pretreatment on antioxidative activity,endogenous hormones and ultrastructure of indica-japonica hybrid rice seedlings 被引量:4
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作者 ZENG Yan-hua zahng yu-ping +4 位作者 XIANG Jing WU Hui CHEN Hui-zhe ZHANG Yi-kai ZHU De-feng 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2016年第2期295-308,共14页
Spermidine(Spd) is known to be involved in the regulation of plant responses to chilling stress and counteract the adverse effect of stress conditions.Antioxidant activities,endogenous hormones and ultrastructure ch... Spermidine(Spd) is known to be involved in the regulation of plant responses to chilling stress and counteract the adverse effect of stress conditions.Antioxidant activities,endogenous hormones and ultrastructure change under chilling stress were investigated in indica-japonica hybrid rice seedlings.12-d-old seedlings were subjected to exogenous Spd(1 mmol L^(-1)) and then a chilling stress(6℃,4 d) was induced,followed by a subsequent recovery(25℃,4 d).Results showed that malondialdehyde(MDA) and proline content were enhanced significantly,whereas shoot fresh and dry weights decreased during chilling stress and after recovery;chlorophyll content of chilling-stressed seedlings increased slightly but declined after recovery;additionally,total soluble sugar,sucrose,fructose and starch contents increased significantly during chilling stress,and only soluble sugar and fructose contents were observed in increase after recovery;chilling stress-induced increases in superoxide dismutase(SOD),peroxidase(POD) and catalase(CAT) activities,but declined after recovery,and the level of ascorbate peroxidase was lower during chilling stress and after recovery;however,endogenous indole-3-acetic acid(IAA),zeatin riboside(ZR),gibberellic acid(GA_3),and abscisic acid(ABA) levels were induced decreased compared with Spd pretreatment.The microscopic analysis revealed that chilling stress-induced destruction of the chloroplast envelope during chilling stress and increased the number of plastoglobuli along with aberrations in thylakoid membranes after recovery.In contrast,exogenous Spd protected rice seedlings from chilling-induced injuries in terms of lower malondialdehyde,proline and carbohydrates accumulation coupled with increased endogenous hormones metabolism.After recovery,Spd pretreatment chilling-exposed seedlings showed higher activities of antioxidant enzymes and normal physiological function of chloroplasts.These results suggest that Spd could promote effectively chilling tolerance which might be largely attributable to the integrity of cell structure and normal metabolism of endogenous hormones in indica-japonica hybrid rice seedlings. 展开更多
关键词 polyamines chilling stress antioxidative activity endogenous hormones ultrastructure indica-japonica hybrid rice(Oryza sativa L.)
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