Objeαive： To determine whether Interferon-alpha-2b （IFN-α2b） can modulate the autophagic response in hepatocellular carcinoma cells. Methods： Hepatocellular carcinoma cells were treated with IFN-α2b. Autophagy ...Objeαive： To determine whether Interferon-alpha-2b （IFN-α2b） can modulate the autophagic response in hepatocellular carcinoma cells. Methods： Hepatocellular carcinoma cells were treated with IFN-α2b. Autophagy was assessed by acridine orange staining, GFP-LC3 dotted assay, transmission eleαron microscopy and immunoblotting. Results： Acridine orange staining showed that IFN-α2b triggered the accumulation of acidic vesicular and autolysosomes in HepG2 cells. The acridine orange HepG2 cell ratios were （4.3±1.0）%, （6.9±1.4）%, and （13.1±2.3）%, respeαively, after treatment with 100, 1,000, and 10,000 IU/mL IFN-α2b for 48 h. A markedly punαate pattern was observed in HepG2 cells treated with 10,000 IU/mL [FN-α2b for 48 h, but only diffuse and weakly fluorescent GFP-LC3 punαa was observed in control cells. HepG2 cells treated with 10,000 IU/mL IFN-α2b for 48 h developed autophagosome-like charaαeristics, including single- or double-membrane vacuoles containing intaα and degraded cellular debris. The Beclinl and LC3-II protein expression was up-regulated by IFN-α2b treatment. Conclusion： Autophagy can be induced in a dose-dependent manner by treatment with IFN-α2b in HepG2 cells, and the Beclinl signaling pathway was stimulated by IFN-α2b.展开更多
Root-knot nematodes(RKNs) cause huge yield losses to agricultural crops worldwide. Meanwhile, livestock manure is often improperly managed by farmers, which leads to serious environmental pollution. To resolve these t...Root-knot nematodes(RKNs) cause huge yield losses to agricultural crops worldwide. Meanwhile, livestock manure is often improperly managed by farmers, which leads to serious environmental pollution. To resolve these two problems, this study developed a procedure for the conversion of chicken manure to organic fertilizer by larvae of Hermetia illucens L. and Bacillus subtilis BSF-CL. Chicken manure organic fertilizer was then mixed thoroughly with Paenibacillus polymyxa KM2501-1 to a final concentration of 1.5×10^(8)CFU g^(-1). The efficacy of KM2501-1 microbial organic fertilizer in controlling root-knot nematodes was evaluated in pot and field experiments. In pot experiments, applying KM2501-1 microbial organic fertilizer either as a base fertilizer or as a fumigant at the dose of 40 g/pot suppressed root-knot disease by 61.76 and 69.05% compared to the corresponding control treatments, respectively. When applied as a fumigant at the dose of 1 kg m;in field experiments, KM2501-1 microbial organic fertilizer enhanced the growth of tomato plants, suppressed root-knot disease by 49.97%, and reduced second stage juveniles of RKN in soil by 88.68%. KM2501-1 microbial organic fertilizer controlled RKNs better than commercial bio-organic fertilizer in both pot and field experiments. These results demonstrate that this co-conversion process efficiently transforms chicken manure into high value-added larvae biomass and KM2501-1 microbial organic fertilizer with potential application as a novel nematode control agent.展开更多
A micro-displacement sensor based on fiber Bragg grating(FBG) is proposed. The device consists of a pair of FBGs with different central wavelengths fabricated by femtosecond laser phase mask method and a metal substra...A micro-displacement sensor based on fiber Bragg grating(FBG) is proposed. The device consists of a pair of FBGs with different central wavelengths fabricated by femtosecond laser phase mask method and a metal substrate with lever structure. The displacement is amplified by lever structure and it converts into axial tension of FBG, which has a high displacement sensitivity. The amplification factors obtained by theoretical analysis and finite element simulation are 2.67 and 2.50, respectively. The experimental results show that in the range of 0-50 μm the shift of FBG center wavelength is linearly related to the displacement of measured object and displacement sensitivity reaches 121 pm/μm. In addition, the cascaded FBG is used to compensate the temperature.展开更多
The flake-like basic magnesium carbonate was prepared from the ammonium sulfate leaching liquid of asbestos tailings. The method of X-ray diffraction, Fourier transform infrared spectra, TA-DTG and SEM analysis had be...The flake-like basic magnesium carbonate was prepared from the ammonium sulfate leaching liquid of asbestos tailings. The method of X-ray diffraction, Fourier transform infrared spectra, TA-DTG and SEM analysis had been used to research the influence of the reaction temperature on the phase, structure and morphology of the product during the preparation of the basic magnesium carbonate. The results indicates that the product was needle-like magnesium carbonate hydrate at the lower reaction temperature (from 35 ℃ to 65℃), with the increase of the reaction temperature (75 ℃ to 95 ℃ ), the needle-like magnesium carbonate hydrate (MgCO3·xH2O) transformed to flake-like basic magnesium carbonate [Mg5(CO3)4(OH)2·4H2O], the reaction temperature plays an important role in the preparation of flake-like basic magnesium carbonate, the preparation of flake-like basic magnesium carbonate was a nucleation-dissolution-recrystallization growth mechanism process.展开更多
This paper focuses on the extraction of a harmonic signal from multiplicative and additive noises. A method is proposed in two stages： （1） to square the original discrete time series, which includes both signals an...This paper focuses on the extraction of a harmonic signal from multiplicative and additive noises. A method is proposed in two stages： （1） to square the original discrete time series, which includes both signals and noises, and form a new time series. By this means, the multiplicative noise is converted to additive noise; and （2） to filter out the noise by using existing noise removal schemes. With a large amount of simulation, experimental results demonstrated the efficiency and effectiveness of this newly developed method in terms of Signal-to-Noise Ratio （SNR） and other criteria. Prom the experiment, it is also found that： the two kinds of noises affect the SNR differently. In general, the SNR is not influenced by multiplicative Gaussian noise regardless of its variance. However, if both kinds of noise exist, the SNR decreases with the incensement of the Variance of Additive Noise to Multiplicative Noise Ratio （VAMNR）. This analysis is also supported by simulation work.展开更多
[Objective]The paper was to understand the field resistance performance of different banana varieties and the prevention and control ef-fect of different fertilizers,so as to provide technical reference for the preven...[Objective]The paper was to understand the field resistance performance of different banana varieties and the prevention and control ef-fect of different fertilizers,so as to provide technical reference for the prevention and control of Fusarium wilt.[Method]Field trials were set up with three treatments:shrimp peptide organic fertilizer+shrimp peptide special protection+shrimp peptide fruit Yekang(simplified as shrimp peptide organic fertilizer treatment),conventional organic fertilizer+microbial preparations(simplified as microbial treatment),and conventional or-ganic fertilizer(simplified as control).Four different banana varieties of Brazilian banana,Guijiao No.1,Nantianhuang,and Yunjiao No.1 were se-lected for the field trial.The disease incidence of Fusarium wilt and the control effects of three fertilizers were investigated during four time periods.[Result]The disease incidence of four varieties in three treatments varied.The disease incidence of Nantianhuang and Yunjiao No.1 were signifi-cantly lower than that of other two varieties.There was also significant difference in disease incidence of three treatments.The disease incidence from high to low was control>shrimp peptide organic fertilizer treatment>microbial treatment.The average monthly TR4 pathogen content in heavily infected banana plantation was more than 2000 copies,while the highest one reached 15148.9 copies.[Conclusion]Microbial agents reduced the disease incidence of Fusarium wilt to some content.Nantianhuang and Yunjiao No.1 showed the highest disease resistance compared with other varieties.However,their resistance needs to be further improved before practical application.展开更多
Based on the sample data of the listed firms on the A stock market, we analyze how the system of independent directors influence the earning conservatism from 4 dimensions designed to measure the monitoring power of t...Based on the sample data of the listed firms on the A stock market, we analyze how the system of independent directors influence the earning conservatism from 4 dimensions designed to measure the monitoring power of these independent directors： Percentage within BDs, professional capacities, stimulation and work conditions. From the empirical results, we conclude that the more powerful of the independent directors, the better the accounting conservatism, and that their positive impact increases along with the improvement on corporate governance, meanwhile the work conditions plays the most important role, and then are the percentage within BDs and their professional capacities, the stimulation and personal reputation appear the least importance.展开更多
基金supported by grants from the Scientific Support Project of Anhui Province Education Department of China (Grant No. KJ2012ZD08 and KJ2012Z162)the National Scientific and Technological Support Projects of China (Grant No. 81101273)the National Natural Science Foundation of China (Grant No. 30872253)
文摘Objeαive： To determine whether Interferon-alpha-2b （IFN-α2b） can modulate the autophagic response in hepatocellular carcinoma cells. Methods： Hepatocellular carcinoma cells were treated with IFN-α2b. Autophagy was assessed by acridine orange staining, GFP-LC3 dotted assay, transmission eleαron microscopy and immunoblotting. Results： Acridine orange staining showed that IFN-α2b triggered the accumulation of acidic vesicular and autolysosomes in HepG2 cells. The acridine orange HepG2 cell ratios were （4.3±1.0）%, （6.9±1.4）%, and （13.1±2.3）%, respeαively, after treatment with 100, 1,000, and 10,000 IU/mL IFN-α2b for 48 h. A markedly punαate pattern was observed in HepG2 cells treated with 10,000 IU/mL [FN-α2b for 48 h, but only diffuse and weakly fluorescent GFP-LC3 punαa was observed in control cells. HepG2 cells treated with 10,000 IU/mL IFN-α2b for 48 h developed autophagosome-like charaαeristics, including single- or double-membrane vacuoles containing intaα and degraded cellular debris. The Beclinl and LC3-II protein expression was up-regulated by IFN-α2b treatment. Conclusion： Autophagy can be induced in a dose-dependent manner by treatment with IFN-α2b in HepG2 cells, and the Beclinl signaling pathway was stimulated by IFN-α2b.
基金supported by the project from China Ocean Mineral Resources R&D Association(COMRADY135-B2-17)+1 种基金the National Key Technologies R&D Program of China(2018YFD0500203 and 2018YFF0213503)the Major Projects of Technological Innovation in Hubei Province,China(2017ABA072)。
文摘Root-knot nematodes(RKNs) cause huge yield losses to agricultural crops worldwide. Meanwhile, livestock manure is often improperly managed by farmers, which leads to serious environmental pollution. To resolve these two problems, this study developed a procedure for the conversion of chicken manure to organic fertilizer by larvae of Hermetia illucens L. and Bacillus subtilis BSF-CL. Chicken manure organic fertilizer was then mixed thoroughly with Paenibacillus polymyxa KM2501-1 to a final concentration of 1.5×10^(8)CFU g^(-1). The efficacy of KM2501-1 microbial organic fertilizer in controlling root-knot nematodes was evaluated in pot and field experiments. In pot experiments, applying KM2501-1 microbial organic fertilizer either as a base fertilizer or as a fumigant at the dose of 40 g/pot suppressed root-knot disease by 61.76 and 69.05% compared to the corresponding control treatments, respectively. When applied as a fumigant at the dose of 1 kg m;in field experiments, KM2501-1 microbial organic fertilizer enhanced the growth of tomato plants, suppressed root-knot disease by 49.97%, and reduced second stage juveniles of RKN in soil by 88.68%. KM2501-1 microbial organic fertilizer controlled RKNs better than commercial bio-organic fertilizer in both pot and field experiments. These results demonstrate that this co-conversion process efficiently transforms chicken manure into high value-added larvae biomass and KM2501-1 microbial organic fertilizer with potential application as a novel nematode control agent.
基金Projects(51875585, 51875584, 51935013) supported by the National Natural Science Foundation of ChinaProject(2020JJ4247) supported by the Natural Science Foundation of Hunan Province,ChinaProject(ZHD202001) supported by the Opening Project of Science and Technology on Reliability Physics and Application Technology of Electronic Component Laboratory,China。
文摘A micro-displacement sensor based on fiber Bragg grating(FBG) is proposed. The device consists of a pair of FBGs with different central wavelengths fabricated by femtosecond laser phase mask method and a metal substrate with lever structure. The displacement is amplified by lever structure and it converts into axial tension of FBG, which has a high displacement sensitivity. The amplification factors obtained by theoretical analysis and finite element simulation are 2.67 and 2.50, respectively. The experimental results show that in the range of 0-50 μm the shift of FBG center wavelength is linearly related to the displacement of measured object and displacement sensitivity reaches 121 pm/μm. In addition, the cascaded FBG is used to compensate the temperature.
文摘The flake-like basic magnesium carbonate was prepared from the ammonium sulfate leaching liquid of asbestos tailings. The method of X-ray diffraction, Fourier transform infrared spectra, TA-DTG and SEM analysis had been used to research the influence of the reaction temperature on the phase, structure and morphology of the product during the preparation of the basic magnesium carbonate. The results indicates that the product was needle-like magnesium carbonate hydrate at the lower reaction temperature (from 35 ℃ to 65℃), with the increase of the reaction temperature (75 ℃ to 95 ℃ ), the needle-like magnesium carbonate hydrate (MgCO3·xH2O) transformed to flake-like basic magnesium carbonate [Mg5(CO3)4(OH)2·4H2O], the reaction temperature plays an important role in the preparation of flake-like basic magnesium carbonate, the preparation of flake-like basic magnesium carbonate was a nucleation-dissolution-recrystallization growth mechanism process.
基金Supported by the Natural Science Foundation of Shaanxi Province (No.2003F40).
文摘This paper focuses on the extraction of a harmonic signal from multiplicative and additive noises. A method is proposed in two stages： （1） to square the original discrete time series, which includes both signals and noises, and form a new time series. By this means, the multiplicative noise is converted to additive noise; and （2） to filter out the noise by using existing noise removal schemes. With a large amount of simulation, experimental results demonstrated the efficiency and effectiveness of this newly developed method in terms of Signal-to-Noise Ratio （SNR） and other criteria. Prom the experiment, it is also found that： the two kinds of noises affect the SNR differently. In general, the SNR is not influenced by multiplicative Gaussian noise regardless of its variance. However, if both kinds of noise exist, the SNR decreases with the incensement of the Variance of Additive Noise to Multiplicative Noise Ratio （VAMNR）. This analysis is also supported by simulation work.
基金Supported by Major Crop Pests Monitoring,Disaster Regulation and Prevention and Control Mecha-nism Project in Yunnan Province(2018BB016)Yunling Scholar Programme of Yunnan Provincial Government,Science and Technology Innovation Talents Program in Yunnan Province(2015HA034)+1 种基金Special Project of National Banana Industry Technical System(CARS-31-08)Key Laboratory of Green Prevention and Control of Agricultural Transboundary Pests of Yunnan Province&the CGIAR Research Program on Roots,Tubers and Bananas(RTB)。
文摘[Objective]The paper was to understand the field resistance performance of different banana varieties and the prevention and control ef-fect of different fertilizers,so as to provide technical reference for the prevention and control of Fusarium wilt.[Method]Field trials were set up with three treatments:shrimp peptide organic fertilizer+shrimp peptide special protection+shrimp peptide fruit Yekang(simplified as shrimp peptide organic fertilizer treatment),conventional organic fertilizer+microbial preparations(simplified as microbial treatment),and conventional or-ganic fertilizer(simplified as control).Four different banana varieties of Brazilian banana,Guijiao No.1,Nantianhuang,and Yunjiao No.1 were se-lected for the field trial.The disease incidence of Fusarium wilt and the control effects of three fertilizers were investigated during four time periods.[Result]The disease incidence of four varieties in three treatments varied.The disease incidence of Nantianhuang and Yunjiao No.1 were signifi-cantly lower than that of other two varieties.There was also significant difference in disease incidence of three treatments.The disease incidence from high to low was control>shrimp peptide organic fertilizer treatment>microbial treatment.The average monthly TR4 pathogen content in heavily infected banana plantation was more than 2000 copies,while the highest one reached 15148.9 copies.[Conclusion]Microbial agents reduced the disease incidence of Fusarium wilt to some content.Nantianhuang and Yunjiao No.1 showed the highest disease resistance compared with other varieties.However,their resistance needs to be further improved before practical application.
文摘Based on the sample data of the listed firms on the A stock market, we analyze how the system of independent directors influence the earning conservatism from 4 dimensions designed to measure the monitoring power of these independent directors： Percentage within BDs, professional capacities, stimulation and work conditions. From the empirical results, we conclude that the more powerful of the independent directors, the better the accounting conservatism, and that their positive impact increases along with the improvement on corporate governance, meanwhile the work conditions plays the most important role, and then are the percentage within BDs and their professional capacities, the stimulation and personal reputation appear the least importance.
