Shaanxi Province is rich in archaeological heritage.Its capital,Xi’an,was the capital of 13 dynasties up until the end of the Tang Dynasty(618-907),nine of which were stable,united,multiethnic societies that created ...Shaanxi Province is rich in archaeological heritage.Its capital,Xi’an,was the capital of 13 dynasties up until the end of the Tang Dynasty(618-907),nine of which were stable,united,multiethnic societies that created rich cultures and left behind an abundance of precious relics.展开更多
China is pushing to develop child-friendly cities Li Jingyu,a 10-year-old student from Peixin Primary School in Beijing’s Dongcheng District,has been an art enthusiast for six years.On 3 November,one of her creations...China is pushing to develop child-friendly cities Li Jingyu,a 10-year-old student from Peixin Primary School in Beijing’s Dongcheng District,has been an art enthusiast for six years.On 3 November,one of her creations was exhibited at the capital’s Today Art Museum.展开更多
目的探讨银杏叶提取物EGB761对糖尿病肾病(DKD)小鼠肾小管损伤及内质网应激的影响。方法将50只C57BL/6N小鼠随机分为正常组、DKD模型组、DKD+EGB761组(36 mg·kg-1)以及DKD+4-苯基丁酸(4-PBA)组(1 g·kg-1)。通过高脂饲料联合...目的探讨银杏叶提取物EGB761对糖尿病肾病(DKD)小鼠肾小管损伤及内质网应激的影响。方法将50只C57BL/6N小鼠随机分为正常组、DKD模型组、DKD+EGB761组(36 mg·kg-1)以及DKD+4-苯基丁酸(4-PBA)组(1 g·kg-1)。通过高脂饲料联合腹腔注射链脲佐菌素(STZ)制备糖尿病小鼠模型,糖尿病模型复制成功并继续高脂饲料喂养8周后检测各组尿蛋白,阳性者给药8周,给药期间观察各组小鼠一般情况。给药8周后检测血肌酐(Scr)、尿素氮(BUN)、24 h尿蛋白(24 h Pro)、空腹血糖(FBG)、肾脏肥大指数;HE染色、Masson染色、PAS染色观察各组小鼠肾脏病理改变;透射电镜观察肾小球及肾小管超微结构;ELLSA法检测各组小鼠肾小管损伤标志物尿β2-微球蛋白(β2-MG)、视黄醇结合蛋白4(RBP4)、中性粒细胞明胶酶相关脂质运载蛋白(NGAL)的表达;以免疫组化、蛋白免疫印迹法(Western blot)、实时荧光定量聚合酶链式反应(Realtime-PCR)检测肾组织内质网应激标志物GRP78、ATF6的表达。结果与正常组比,DKD模型组精神状态较差、反应迟钝、多饮多食多尿症状明显,Scr、BUN、24 h Pro、FBG、肾脏肥大指数均明显升高(P<0.01),肾脏病理损伤加重,尿β2-MG、RBP4、NGAL明显升高(P<0.01),肾组织GRP78、ATF6的表达明显升高(P<0.01)。与DKD模型组相比,DKD+EGB761组精神状态、反应情况及多饮多食多尿症状好转,Scr、BUN、24 h Pro、FBG水平均明显降低(P<0.01),肾脏肥大指数明显降低(P<0.05),肾脏病理损伤明显改善,尿β2-MG、RBP4、NGAL表达降低(P<0.05,P<0.01),肾组织GRP78、ATF6蛋白的表达降低(P<0.05,P<0.01)。结论银杏叶提取物EGB761可以改善DKD小鼠肾功能,减少蛋白尿,减轻肾小管损伤,其作用机制可能与抑制内质网应激相关。展开更多
文摘Shaanxi Province is rich in archaeological heritage.Its capital,Xi’an,was the capital of 13 dynasties up until the end of the Tang Dynasty(618-907),nine of which were stable,united,multiethnic societies that created rich cultures and left behind an abundance of precious relics.
文摘China is pushing to develop child-friendly cities Li Jingyu,a 10-year-old student from Peixin Primary School in Beijing’s Dongcheng District,has been an art enthusiast for six years.On 3 November,one of her creations was exhibited at the capital’s Today Art Museum.
文摘目的探讨银杏叶提取物EGB761对糖尿病肾病(DKD)小鼠肾小管损伤及内质网应激的影响。方法将50只C57BL/6N小鼠随机分为正常组、DKD模型组、DKD+EGB761组(36 mg·kg-1)以及DKD+4-苯基丁酸(4-PBA)组(1 g·kg-1)。通过高脂饲料联合腹腔注射链脲佐菌素(STZ)制备糖尿病小鼠模型,糖尿病模型复制成功并继续高脂饲料喂养8周后检测各组尿蛋白,阳性者给药8周,给药期间观察各组小鼠一般情况。给药8周后检测血肌酐(Scr)、尿素氮(BUN)、24 h尿蛋白(24 h Pro)、空腹血糖(FBG)、肾脏肥大指数;HE染色、Masson染色、PAS染色观察各组小鼠肾脏病理改变;透射电镜观察肾小球及肾小管超微结构;ELLSA法检测各组小鼠肾小管损伤标志物尿β2-微球蛋白(β2-MG)、视黄醇结合蛋白4(RBP4)、中性粒细胞明胶酶相关脂质运载蛋白(NGAL)的表达;以免疫组化、蛋白免疫印迹法(Western blot)、实时荧光定量聚合酶链式反应(Realtime-PCR)检测肾组织内质网应激标志物GRP78、ATF6的表达。结果与正常组比,DKD模型组精神状态较差、反应迟钝、多饮多食多尿症状明显,Scr、BUN、24 h Pro、FBG、肾脏肥大指数均明显升高(P<0.01),肾脏病理损伤加重,尿β2-MG、RBP4、NGAL明显升高(P<0.01),肾组织GRP78、ATF6的表达明显升高(P<0.01)。与DKD模型组相比,DKD+EGB761组精神状态、反应情况及多饮多食多尿症状好转,Scr、BUN、24 h Pro、FBG水平均明显降低(P<0.01),肾脏肥大指数明显降低(P<0.05),肾脏病理损伤明显改善,尿β2-MG、RBP4、NGAL表达降低(P<0.05,P<0.01),肾组织GRP78、ATF6蛋白的表达降低(P<0.05,P<0.01)。结论银杏叶提取物EGB761可以改善DKD小鼠肾功能,减少蛋白尿,减轻肾小管损伤,其作用机制可能与抑制内质网应激相关。
