目的:研究不同剂量依巴斯汀联合复方甘草酸苷递减疗法治疗慢性荨麻疹的临床应用效果。方法:选取2022年1月至2023年9月我院就诊的44例慢性荨麻疹患者为研究对象。按照不同治疗方案分为对照组和观察组,各22例。对照组采用10 mg依巴斯汀片...目的:研究不同剂量依巴斯汀联合复方甘草酸苷递减疗法治疗慢性荨麻疹的临床应用效果。方法:选取2022年1月至2023年9月我院就诊的44例慢性荨麻疹患者为研究对象。按照不同治疗方案分为对照组和观察组,各22例。对照组采用10 mg依巴斯汀片,观察组采用20 mg依巴斯汀,两组均联合复方甘草酸苷递减疗法治疗。比较两组治疗前后临床症状积分、血液学指标[血清白介素-17(Interleukin-17,IL-17)、白介素-25(Interleukin-25,IL-25)、白介素-33(Interleukin,33,IL-33)]、T淋巴细胞计数[辅助性T细胞(Helper T cell,CD4^(+))、细胞毒性T细胞(Cytotoxic T cell,CD8^(+))]水平、临床疗效及用药不良反应发生率。结果:治疗后,观察组临床症状积分总有效率均高于对照组(P<0.05);观察组总有效率均高于对照组(P<0.05);治疗后,观察组IL-17、IL-25、IL-33及CD8^(+)均低于对照组,CD4^(+)高于对照组(P<0.05);两组不良反应发生率无显著差异。结论:20 mg依巴斯汀联合复方甘草酸苷递减疗法可以更有效缓解慢性荨麻疹患者临床症状,降低血清IL-17、IL-25、IL-33水平,改善机体免疫功能,且较为安全。展开更多
The application of large amounts of fertilizers, a conventional practice in northern China for the production of vegetable crops, generally leads to substantial accumulation of soil nutrients within a relatively short...The application of large amounts of fertilizers, a conventional practice in northern China for the production of vegetable crops, generally leads to substantial accumulation of soil nutrients within a relatively short period of time. A fixed field experiment was designed to study the effects of nitrogen （N） and potassium （K） fertilizers applied to optimize the yield and quality of typical vegetable crops. Application of N and K fertilizers significantly increased the yields of kidney bean. The largest yields were obtained in the first and second years after application of 1 500 kg N and 300 kg K20 ha^-1. In the third year, however, there was a general decline in yields. Maximum yields occurred when intermediate rates of N and K （750 kg N and 300 kg K20 ha 1） were applied. However, no significant differences were observed in the concentrations of vitamin C （VC） in kidney bean among different years and various rates of fertilizer treatments. Yields of tomato grown in rotation after kidney bean showed significant responses to the application of N and K in the first year. In the second year, the yields of tomato were much lower. This suggested that the application of N fertilizer did not have any effect upon tomato yield, whereas application of K fertilizer did increase the yield. Application of K fertilizer was often associated with increased sugar concentrations.展开更多
Recently, the light-absorbing organic carbon, i.e., brown carbon(Br C), has received an increasing attention, because they could significantly absorb the solar radiation in the range of short wavelengths rather than t...Recently, the light-absorbing organic carbon, i.e., brown carbon(Br C), has received an increasing attention, because they could significantly absorb the solar radiation in the range of short wavelengths rather than the purely scattering effect. Br C is ubiquitous in the troposphere. It could undergo long range transport within the atmospheric circulation. After the deposition on the surface of snow or ice in the cryospheric region, as the major light absorbing impurities with black carbon and dust, Br C could reduce the snow albedo and accelerate the glacier melting. In this context, this paper summarized the current knowledge of Br C(in aerosols and snow) in the cryospheric regions including the Arctic, Antarctic,and Alpines. Although some works have been conducted in those region, the current dataset on the optical properties of Br C like Absorption?ngstr€om Exponent(AAE) and Mass Absorption Efficiency(MAE) is still limited, which hampers stimulating an accurate evaluation of its climate effects. Especially in the Himalayas and Tibetan Plateau, where very limited information concerning Br C is available. Considering biomass burning as a dominant source of Br C, a large amount of emissions from biomass burning in South Asia could reach the Himalayas and Tibetan Plateau, where the climate effect of Br C merits more investigation in the future.展开更多
Construction of a food-grade expression vector for application to lactic acid bacteria（LAB） is of importance for dairy fermentation system. An α-galactosidase（aga） gene encoding an enzyme degrading melibiose was ...Construction of a food-grade expression vector for application to lactic acid bacteria（LAB） is of importance for dairy fermentation system. An α-galactosidase（aga） gene encoding an enzyme degrading melibiose was amplified by PCR from the plasmid p RAF800 of Lactococcus lactis NZ9000. The aga gene was introduced into pMG36 e to substitute the p rimary antibiotic selectable marker of pMG36 e, resulting in construction of a new food-grade expression vector pMG36-aga. To testify the expression efficiency of exogenous gene in pMG36-aga, a 1.5 kb long α-amylase（amy） gene from Ba cillus li cheniformis was cloned by PCR and introduced into the plasmid pMG36-aga. The resultant plasimd pMG36-aga-amy was transformed into L. lactis ML23 by electroporation. The positive clones were selected with the medium containing melibiose as the sole carbon source. Th e selection efficiency of aga was 8.71×103 CFU with a standard deviation of 9.1×102 CFU ？g-1 DNA of pMG36-aga. Furthermore, the SDS-PAGE analysis showed that the pMG36-aga-amy expressed a 56.4 kDa protein which was the same as the putati ve molecular weight of α-amylase. The starch plate assay also indicated that L. lactis ML23 displayed high activity of α-amylase by expressing of amy gene of pMG36-aga-amy.展开更多
文摘目的:研究不同剂量依巴斯汀联合复方甘草酸苷递减疗法治疗慢性荨麻疹的临床应用效果。方法:选取2022年1月至2023年9月我院就诊的44例慢性荨麻疹患者为研究对象。按照不同治疗方案分为对照组和观察组,各22例。对照组采用10 mg依巴斯汀片,观察组采用20 mg依巴斯汀,两组均联合复方甘草酸苷递减疗法治疗。比较两组治疗前后临床症状积分、血液学指标[血清白介素-17(Interleukin-17,IL-17)、白介素-25(Interleukin-25,IL-25)、白介素-33(Interleukin,33,IL-33)]、T淋巴细胞计数[辅助性T细胞(Helper T cell,CD4^(+))、细胞毒性T细胞(Cytotoxic T cell,CD8^(+))]水平、临床疗效及用药不良反应发生率。结果:治疗后,观察组临床症状积分总有效率均高于对照组(P<0.05);观察组总有效率均高于对照组(P<0.05);治疗后,观察组IL-17、IL-25、IL-33及CD8^(+)均低于对照组,CD4^(+)高于对照组(P<0.05);两组不良反应发生率无显著差异。结论:20 mg依巴斯汀联合复方甘草酸苷递减疗法可以更有效缓解慢性荨麻疹患者临床症状,降低血清IL-17、IL-25、IL-33水平,改善机体免疫功能,且较为安全。
基金the National Key Technology R&D Program of China (No.2006BAD17B07)the IndependentInnovation Program of the Shandong Academy of Agricultural Sciences of China (No.2007ycx023-01).
文摘The application of large amounts of fertilizers, a conventional practice in northern China for the production of vegetable crops, generally leads to substantial accumulation of soil nutrients within a relatively short period of time. A fixed field experiment was designed to study the effects of nitrogen （N） and potassium （K） fertilizers applied to optimize the yield and quality of typical vegetable crops. Application of N and K fertilizers significantly increased the yields of kidney bean. The largest yields were obtained in the first and second years after application of 1 500 kg N and 300 kg K20 ha^-1. In the third year, however, there was a general decline in yields. Maximum yields occurred when intermediate rates of N and K （750 kg N and 300 kg K20 ha 1） were applied. However, no significant differences were observed in the concentrations of vitamin C （VC） in kidney bean among different years and various rates of fertilizer treatments. Yields of tomato grown in rotation after kidney bean showed significant responses to the application of N and K in the first year. In the second year, the yields of tomato were much lower. This suggested that the application of N fertilizer did not have any effect upon tomato yield, whereas application of K fertilizer did increase the yield. Application of K fertilizer was often associated with increased sugar concentrations.
基金supported by National Science Foundation of China (41522103, 41501082 and 41225002)Strategic Priority Research Program-Climate Change:Carbon Budget and Relevant Issues (XDA05100105)
文摘Recently, the light-absorbing organic carbon, i.e., brown carbon(Br C), has received an increasing attention, because they could significantly absorb the solar radiation in the range of short wavelengths rather than the purely scattering effect. Br C is ubiquitous in the troposphere. It could undergo long range transport within the atmospheric circulation. After the deposition on the surface of snow or ice in the cryospheric region, as the major light absorbing impurities with black carbon and dust, Br C could reduce the snow albedo and accelerate the glacier melting. In this context, this paper summarized the current knowledge of Br C(in aerosols and snow) in the cryospheric regions including the Arctic, Antarctic,and Alpines. Although some works have been conducted in those region, the current dataset on the optical properties of Br C like Absorption?ngstr€om Exponent(AAE) and Mass Absorption Efficiency(MAE) is still limited, which hampers stimulating an accurate evaluation of its climate effects. Especially in the Himalayas and Tibetan Plateau, where very limited information concerning Br C is available. Considering biomass burning as a dominant source of Br C, a large amount of emissions from biomass burning in South Asia could reach the Himalayas and Tibetan Plateau, where the climate effect of Br C merits more investigation in the future.
基金supported by the National High-Tech R&D Program of China (863 Program, 2006AA10Z317)
文摘Construction of a food-grade expression vector for application to lactic acid bacteria（LAB） is of importance for dairy fermentation system. An α-galactosidase（aga） gene encoding an enzyme degrading melibiose was amplified by PCR from the plasmid p RAF800 of Lactococcus lactis NZ9000. The aga gene was introduced into pMG36 e to substitute the p rimary antibiotic selectable marker of pMG36 e, resulting in construction of a new food-grade expression vector pMG36-aga. To testify the expression efficiency of exogenous gene in pMG36-aga, a 1.5 kb long α-amylase（amy） gene from Ba cillus li cheniformis was cloned by PCR and introduced into the plasmid pMG36-aga. The resultant plasimd pMG36-aga-amy was transformed into L. lactis ML23 by electroporation. The positive clones were selected with the medium containing melibiose as the sole carbon source. Th e selection efficiency of aga was 8.71×103 CFU with a standard deviation of 9.1×102 CFU ？g-1 DNA of pMG36-aga. Furthermore, the SDS-PAGE analysis showed that the pMG36-aga-amy expressed a 56.4 kDa protein which was the same as the putati ve molecular weight of α-amylase. The starch plate assay also indicated that L. lactis ML23 displayed high activity of α-amylase by expressing of amy gene of pMG36-aga-amy.
