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Reservoir characterization of the Odovician oil and gas pools in the Tahe Oilfield, Tarim Basin, Northwest China 被引量:15
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作者 zhangkang WANGDa-rui BryanGHuff 《石油勘探与开发》 SCIE EI CAS CSCD 北大核心 2004年第1期123-126,共4页
About 88. 1% of the proven reserves in the Tahe Oilfield in the Tarim Basin of Northwest China are trapped in Ordovician carbonate reservoirs. These reservoirs are formed by unconnected and interconnected networks of ... About 88. 1% of the proven reserves in the Tahe Oilfield in the Tarim Basin of Northwest China are trapped in Ordovician carbonate reservoirs. These reservoirs are formed by unconnected and interconnected networks of karstic porosity forming a heterogeneous and complex reservoir system. Oil, water and gas characteristics vary significantly in different portions of the Ordovician reservoir. There is no uniform oil/water contact in the field, adding to its complexity.An acid fracture treatment is beneficial in 76% of the wells, stimulating nonproductive wells and enhancing production in other wells by fracturing into unconnected reservoirs and enhancing flow pathways with acid. Acid fracture treatments should be a standard procedure for developing this and similar oil fields. 展开更多
关键词 Tahe OILFIELD TARIM Basin ORDOVICIAN reservoir carbonate acid fracture
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Types of Karst-fractured and Porous Reservoirs in China's Carbonates and the Nature of the Tahe Oilfield in the Tarim Basin 被引量:11
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作者 zhangkang WANGDarui 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2004年第3期866-872,共7页
Almost all the oil and gas reservoirs developed in marine sedimentary strata of China have undergone processes of multi-phase reservoir formation and later modification. The irregular reservoirs are classified into th... Almost all the oil and gas reservoirs developed in marine sedimentary strata of China have undergone processes of multi-phase reservoir formation and later modification. The irregular reservoirs are classified into three types as the Naxi, Tahe and Renqiu ones, increasing successively in the development degree of karstificated pores and fissures and the connection degree of independent reservoirs. In these reservoirs, the unity in the fluid feature, pressure and oil-gas-water interface also increases successively from the Naxi to the Renqiu type. The main body of Ordovician reservoirs of the Tahe Oilfield in the Tarim Basin is a network pool rather than a stratified, massive, stratigraphically-unconformed or weathering-crust one. The fluid nature of oil, gas and water, the interface positions and the pressures, as well as the dynamic conditions of fluids within the reservoirs during the production are all different from those in stratified or massive oil and gas reservoirs. Carbonates in the Akekule uplift and the Tahe Oilfield are assemblages of various types of reservoirs, which have an overall oil-bearing potential and obvious uneven distribution. Testing and producing tests are the major means to evaluate this type of reservoirs and acid fracturing improvement is a key link in petroleum exploration and development. 展开更多
关键词 Tarim Basin Tahe Oilfield carbonate reservoir pore and fissure reservoir network oil and gas trap
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嗜热古菌超高温α-淀粉酶在枯草芽孢杆菌中的分泌表达 被引量:2
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作者 檀瑞婷 张康 吴敬 《基因组学与应用生物学》 CAS CSCD 北大核心 2021年第5期2109-2117,共9页
本研究将来源于嗜热古菌Pyrococcus furiosus的超高温α-淀粉酶基因在枯草芽孢杆菌中成功表达,通过信号肽筛选和伴侣蛋白基因共表达提高超高温α-淀粉酶在枯草芽孢杆菌中的分泌表达,并对重组超高温α-淀粉酶的酶学性质进行初步研究。首... 本研究将来源于嗜热古菌Pyrococcus furiosus的超高温α-淀粉酶基因在枯草芽孢杆菌中成功表达,通过信号肽筛选和伴侣蛋白基因共表达提高超高温α-淀粉酶在枯草芽孢杆菌中的分泌表达,并对重组超高温α-淀粉酶的酶学性质进行初步研究。首先构建信号肽筛选文库,结合高通量筛选方法对枯草来源的173种信号肽进行筛选,最终筛选出一种新的信号肽AspB’,引导PFA胞外分泌表达,摇瓶发酵60 h后其酶活达到119.01 U/mL。在此基础上,共表达伴侣蛋白PrsA,使酶活进一步提高,发酵培养60 h后其酶活进一步提高12.72%,达134.15 U/mL。该菌株发酵潜力较好,摇瓶培养310 h后其酶活可达938.35 U/m L。对枯草芽孢杆菌中重组PFA的酶学性质进行测定,最适反应温度为100℃,最适反应pH值为4.5,在100℃条件下处理4 h仍有50%以上的酶活,在pH值4.5~7.0的条件下其稳定性良好。这在淀粉制糖工艺中具有重要的工业价值。 展开更多
关键词 超高温α-淀粉酶 枯草芽孢杆菌 信号肽 伴侣蛋白 酶学性质
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