To dissect the genetic basis of low phosphorus tolerance （LPT）, 114 BC2F4 introgression lines （ILs） were developed from Shuhui 527 and Minghui 86 （recurrent parents）, and Yetuozai （donor parent）. The progenies...To dissect the genetic basis of low phosphorus tolerance （LPT）, 114 BC2F4 introgression lines （ILs） were developed from Shuhui 527 and Minghui 86 （recurrent parents）, and Yetuozai （donor parent）. The progenies were tested for 11 quantitative traits under three treatments including normal fertilization in normal soil （as control）, normal fertilization in barren soil and low phosphorus stress in barren soil in Langfang, Hebei Province, China. Moreover, the ILs were investigated at the seedling stage using nutrient solution culture method in greenhouse in Beijing, China. A total of 49 main-effect quantitative trait loci （QTLs） underlying yield related traits were identified in Langfang, and their contributions to phenotypic variations ranged from 6.7% to 16.5%. Among them, 25 （51.0%） QTLs had favorable alleles from donor parent. A total of 48 main-effect QTLs were identified for LPT-related traits in Beijing, and their contributions to phenotypic variations ranged from 7.7% to 16.6%. Among them, 21 （43.8%） QTLs had favorable alleles from donor parent. About 79.6% of the QTLs can be detected repeatedly under two or more treatments, especially QTLs associated with spikelet number per panicle, spikelet fertility and 1000-grain weight, displaying consistent phenotypic effects. Among all the detected QTLs, eight QTLs were simultaneously identified under low phosphorus stress across two environments. These results can provide useful information for the genetic dissection of LPT in rice.展开更多
Heading date is a crucial agronomic trait.However,rice usually delays heading due to the photoperiod,temperature,hormones or age.The present research was conducted to analyze the mechanism cotrlling heading date in F,...Heading date is a crucial agronomic trait.However,rice usually delays heading due to the photoperiod,temperature,hormones or age.The present research was conducted to analyze the mechanism cotrlling heading date in F,hybrid rice.We constructed two test-crossing populations using two introgression lines(ILs),P20 and P21 coming from SH527/FH838 as the male parent,respectively,and male sterile line Jin23A as the female parent.Meanwhile,the F,hybrids of H20,obtained by mating P20 with Jin23A and having no heading,and H21,from the crossing between P21 and Jin23A having normal heading,were both observed under long days.Here,we analyzed the photoperiodic response of F,hybrids by transcriptome and metabolome profiling.The greater differences displayed in the transcriptome and the metabolome were caused by photoperiod(exogenous)instead of genes(endogenous).The coping mechanism resulted from long days(LD)in H20,leading to differences in the circadian rhythm and glutathione metabolism relative to other samples.The circadian oscillator and GSH/GSSG cycle typically regulate ROS homeostasis,and both of them are responsible for modulating ROS in H20 under LD condition.Both circadian rhythm genes and the reported genes related to heading date function via the DHD1/OsMFT1-Ehd1-RFT1-OsMADS14/OsMADS18 pathway and the glutathione metabolism pathway by regulating oxidative reduction processes.Both pathways are involved in the heading process and they interacted through the oxidative reduction process which was induced by photoperiod regulation,and all of them collectively modulated the heading process.The results of this study will be helpful for unraveling the mechanism of F,hybrid responses to unheading under LD condition.展开更多
基金funded by the National High-Technology Research and Development Program of China (Grant No.2014AA10A604)Shenzhen Peacock Plan in China
文摘To dissect the genetic basis of low phosphorus tolerance （LPT）, 114 BC2F4 introgression lines （ILs） were developed from Shuhui 527 and Minghui 86 （recurrent parents）, and Yetuozai （donor parent）. The progenies were tested for 11 quantitative traits under three treatments including normal fertilization in normal soil （as control）, normal fertilization in barren soil and low phosphorus stress in barren soil in Langfang, Hebei Province, China. Moreover, the ILs were investigated at the seedling stage using nutrient solution culture method in greenhouse in Beijing, China. A total of 49 main-effect quantitative trait loci （QTLs） underlying yield related traits were identified in Langfang, and their contributions to phenotypic variations ranged from 6.7% to 16.5%. Among them, 25 （51.0%） QTLs had favorable alleles from donor parent. A total of 48 main-effect QTLs were identified for LPT-related traits in Beijing, and their contributions to phenotypic variations ranged from 7.7% to 16.6%. Among them, 21 （43.8%） QTLs had favorable alleles from donor parent. About 79.6% of the QTLs can be detected repeatedly under two or more treatments, especially QTLs associated with spikelet number per panicle, spikelet fertility and 1000-grain weight, displaying consistent phenotypic effects. Among all the detected QTLs, eight QTLs were simultaneously identified under low phosphorus stress across two environments. These results can provide useful information for the genetic dissection of LPT in rice.
基金Supported by the National Basic Research Program of China(2014AA10A604).
文摘Heading date is a crucial agronomic trait.However,rice usually delays heading due to the photoperiod,temperature,hormones or age.The present research was conducted to analyze the mechanism cotrlling heading date in F,hybrid rice.We constructed two test-crossing populations using two introgression lines(ILs),P20 and P21 coming from SH527/FH838 as the male parent,respectively,and male sterile line Jin23A as the female parent.Meanwhile,the F,hybrids of H20,obtained by mating P20 with Jin23A and having no heading,and H21,from the crossing between P21 and Jin23A having normal heading,were both observed under long days.Here,we analyzed the photoperiodic response of F,hybrids by transcriptome and metabolome profiling.The greater differences displayed in the transcriptome and the metabolome were caused by photoperiod(exogenous)instead of genes(endogenous).The coping mechanism resulted from long days(LD)in H20,leading to differences in the circadian rhythm and glutathione metabolism relative to other samples.The circadian oscillator and GSH/GSSG cycle typically regulate ROS homeostasis,and both of them are responsible for modulating ROS in H20 under LD condition.Both circadian rhythm genes and the reported genes related to heading date function via the DHD1/OsMFT1-Ehd1-RFT1-OsMADS14/OsMADS18 pathway and the glutathione metabolism pathway by regulating oxidative reduction processes.Both pathways are involved in the heading process and they interacted through the oxidative reduction process which was induced by photoperiod regulation,and all of them collectively modulated the heading process.The results of this study will be helpful for unraveling the mechanism of F,hybrid responses to unheading under LD condition.
