目的:对比并评估达芬奇机器人手术与传统腹腔镜手术治疗妊娠期附件包块的效果,分析达芬奇机器人手术治疗妊娠期附件包块的安全性及可行性。方法:收集2019年1月—2023年1月郑州大学第一附属医院妇科收治的57例妊娠期附件包块患者的临床资...目的:对比并评估达芬奇机器人手术与传统腹腔镜手术治疗妊娠期附件包块的效果,分析达芬奇机器人手术治疗妊娠期附件包块的安全性及可行性。方法:收集2019年1月—2023年1月郑州大学第一附属医院妇科收治的57例妊娠期附件包块患者的临床资料,按手术方式不同分为机器人组(n=17)和传统腹腔镜组(n=40)。比较两组患者的一般情况资料、围手术期资料、妊娠结局、终止妊娠方式及新生儿结局。结果:两组患者的年龄、体质指数、既往腹部手术史发生率、产次、糖类抗原125、附件包块侧性、附件包块性质、术后并发症发生率、附件包块病理类型相比,差异均无统计学意义(P>0.05)。与传统腹腔镜组相比,机器人组患者孕周更长,附件包块直径更大,急诊手术率更低,手术时间更短,术中估计失血量更少,术后排气时间更短,术后住院时间更短,住院总费用更多。两组患者术中均未发生并发症、输血或中转开腹。两组患者的妊娠结局、终止妊娠方式、新生儿出生孕周、新生儿出生体重、新生儿1 min Apgar评分、新生儿5 min Apgar评分及新生儿并发症发生率相比,差异均无统计学意义(P>0.05)。结论:达芬奇机器人手术治疗妊娠期附件包块是安全、有效、可行的。妊娠期附件包块患者接受达芬奇机器人手术与传统腹腔镜手术术后的妊娠结局、终止妊娠方式和新生儿结局相似。与传统腹腔镜手术相比,即使在更大的妊娠期子宫和(或)更大的附件包块而导致手术难度较高的情况下,达芬奇机器人手术仍能表现出手术时间更短、术中出血量更少、术后肠道功能恢复更快、术后住院时间更短等优势。展开更多
双供水供气系统是保证结冰风洞实现过冷大水滴(Super-cooled Large Droplet,SLD)云雾模拟能力的关键,针对现有喷雾系统存在SLD云雾模拟能力不足、运行包线有待扩展等问题,研制了一套大型结冰风洞双供水供气喷雾控制系统。针对喷雾系统...双供水供气系统是保证结冰风洞实现过冷大水滴(Super-cooled Large Droplet,SLD)云雾模拟能力的关键,针对现有喷雾系统存在SLD云雾模拟能力不足、运行包线有待扩展等问题,研制了一套大型结冰风洞双供水供气喷雾控制系统。针对喷雾系统控制变量多、测量点数大等特点,基于工业以太网络建立分布式控制系统,制定了试验流程、方法与策略,建立了完善的双供水供气喷雾试验技术,并开展了系统调试与验证。试验结果表明,双路水压、气压控制精度均稳定在1%以内,达到了系统设计指标,该系统可为后续我国飞行器适航审定提供有力支撑。展开更多
BACKGROUND Glioma is one of the most common intracranial tumors,characterized by invasive growth and poor prognosis.Actin cytoskeletal rearrangement is an essential event of tumor cell migration.The actin dynamics-rel...BACKGROUND Glioma is one of the most common intracranial tumors,characterized by invasive growth and poor prognosis.Actin cytoskeletal rearrangement is an essential event of tumor cell migration.The actin dynamics-related protein scinderin(SCIN)has been reported to be closely related to tumor cell migration and invasion in several cancers.AIM To investigate the role and mechanism of SCIN in glioma.METHODS The expression and clinical significance of SCIN in glioma were analyzed based on public databases.SCIN expression was examined using real-time quantitative polymerase chain reaction and Western blotting.Gene silencing was performed using short hairpin RNA transfection.Cell viability,migration,and invasion were assessed using cell counting kit 8 assay,wound healing,and Matrigel invasion assays,respectively.F-actin cytoskeleton organization was assessed using F-actin staining.RESULTS SCIN expression was significantly elevated in glioma,and high levels of SCIN were associated with advanced tumor grade and wild-type isocitrate dehydrogenase.Furthermore,SCIN-deficient cells exhibited decreased proliferation,migration,and invasion in U87 and U251 cells.Moreover,knockdown of SCIN inhibited the RhoA/focal adhesion kinase(FAK)signaling to promote F-actin depolymerization in U87 and U251 cells.CONCLUSION SCIN modulates the actin cytoskeleton via activating RhoA/FAK signaling,thereby promoting the migration and invasion of glioma cells.This study identified the cancer-promoting effect of SCIN and provided a potential therapeutic target for the treatment of glioma.展开更多
文摘目的:对比并评估达芬奇机器人手术与传统腹腔镜手术治疗妊娠期附件包块的效果,分析达芬奇机器人手术治疗妊娠期附件包块的安全性及可行性。方法:收集2019年1月—2023年1月郑州大学第一附属医院妇科收治的57例妊娠期附件包块患者的临床资料,按手术方式不同分为机器人组(n=17)和传统腹腔镜组(n=40)。比较两组患者的一般情况资料、围手术期资料、妊娠结局、终止妊娠方式及新生儿结局。结果:两组患者的年龄、体质指数、既往腹部手术史发生率、产次、糖类抗原125、附件包块侧性、附件包块性质、术后并发症发生率、附件包块病理类型相比,差异均无统计学意义(P>0.05)。与传统腹腔镜组相比,机器人组患者孕周更长,附件包块直径更大,急诊手术率更低,手术时间更短,术中估计失血量更少,术后排气时间更短,术后住院时间更短,住院总费用更多。两组患者术中均未发生并发症、输血或中转开腹。两组患者的妊娠结局、终止妊娠方式、新生儿出生孕周、新生儿出生体重、新生儿1 min Apgar评分、新生儿5 min Apgar评分及新生儿并发症发生率相比,差异均无统计学意义(P>0.05)。结论:达芬奇机器人手术治疗妊娠期附件包块是安全、有效、可行的。妊娠期附件包块患者接受达芬奇机器人手术与传统腹腔镜手术术后的妊娠结局、终止妊娠方式和新生儿结局相似。与传统腹腔镜手术相比,即使在更大的妊娠期子宫和(或)更大的附件包块而导致手术难度较高的情况下,达芬奇机器人手术仍能表现出手术时间更短、术中出血量更少、术后肠道功能恢复更快、术后住院时间更短等优势。
文摘双供水供气系统是保证结冰风洞实现过冷大水滴(Super-cooled Large Droplet,SLD)云雾模拟能力的关键,针对现有喷雾系统存在SLD云雾模拟能力不足、运行包线有待扩展等问题,研制了一套大型结冰风洞双供水供气喷雾控制系统。针对喷雾系统控制变量多、测量点数大等特点,基于工业以太网络建立分布式控制系统,制定了试验流程、方法与策略,建立了完善的双供水供气喷雾试验技术,并开展了系统调试与验证。试验结果表明,双路水压、气压控制精度均稳定在1%以内,达到了系统设计指标,该系统可为后续我国飞行器适航审定提供有力支撑。
文摘BACKGROUND Glioma is one of the most common intracranial tumors,characterized by invasive growth and poor prognosis.Actin cytoskeletal rearrangement is an essential event of tumor cell migration.The actin dynamics-related protein scinderin(SCIN)has been reported to be closely related to tumor cell migration and invasion in several cancers.AIM To investigate the role and mechanism of SCIN in glioma.METHODS The expression and clinical significance of SCIN in glioma were analyzed based on public databases.SCIN expression was examined using real-time quantitative polymerase chain reaction and Western blotting.Gene silencing was performed using short hairpin RNA transfection.Cell viability,migration,and invasion were assessed using cell counting kit 8 assay,wound healing,and Matrigel invasion assays,respectively.F-actin cytoskeleton organization was assessed using F-actin staining.RESULTS SCIN expression was significantly elevated in glioma,and high levels of SCIN were associated with advanced tumor grade and wild-type isocitrate dehydrogenase.Furthermore,SCIN-deficient cells exhibited decreased proliferation,migration,and invasion in U87 and U251 cells.Moreover,knockdown of SCIN inhibited the RhoA/focal adhesion kinase(FAK)signaling to promote F-actin depolymerization in U87 and U251 cells.CONCLUSION SCIN modulates the actin cytoskeleton via activating RhoA/FAK signaling,thereby promoting the migration and invasion of glioma cells.This study identified the cancer-promoting effect of SCIN and provided a potential therapeutic target for the treatment of glioma.
