[ Objective] To observe the expression of telomerase in antler of sika deer during growth stage and thus to provide a new line for revealing the mechanisms of development and regeneration of antlers. [Method] The upda...[ Objective] To observe the expression of telomerase in antler of sika deer during growth stage and thus to provide a new line for revealing the mechanisms of development and regeneration of antlers. [Method] The updated TRAP （Telomedc repeat amplification protocol） argentumdying method was used to detect the activity of telomerase in mesenchyme, precartilage, cartilage and ossification tissue of antler tip during the growth stage. Reverse transcription polymerase chain reaction （RT-PCR） was used to detect the expression of telomerase reverse transcriptase （TERT） in these cells. I-Result] The activity of telomerase was detected in the mesanchyme, precartilage and cartilage but not in the sclerotic tissue. The relative telomerase activity in the mesanchyme, precartilage and cartilage was 91.2, 46.4 and 13.7, respectively. In addition, the results of the expression of TERT gene also supported the detection result of telomerase activity. I-Conclmionl During growth phase, telomerase is expressed in multiplication area of deer antlers, and its activity decreases gradually with further differentiation of cells in antlers. Therefore, telomerase may play a key role in develoomant and rsoaneration of antlers.展开更多
Background The purpose of this study was to investigate the feasibility of avoiding axillary lymph node dissection （ALND） for patients with only one sentinel lymph node （SLN） metastasis. The characteristics and pr...Background The purpose of this study was to investigate the feasibility of avoiding axillary lymph node dissection （ALND） for patients with only one sentinel lymph node （SLN） metastasis. The characteristics and predictive factors for non-sentinel lymph node （NSLN） metastasis of patients with single positive SLN were also analyzed. Methods Patients with no and only one SLN metastasis （OIn and 1In group, n ≥2） were selected from 1228 cases of invasive breast carcinoma, who underwent axillary dissection in Shandong Cancer Hospital between November 1999 and December 2011, to compare the characteristics of NSLN metastasis between them. For the 1In group, the factors that influenced the NSLN metastasis were analyzed by univariate and multivariate analysis. Results Differences of the NSLN metastasis between the OIn and the 1In groups were significant （P 〈0.001）. There was no significant difference between the axillary lymph node metastasis on level III in 1In group and OIn group （P=0.570）. When the total SLN number was 〉4 and with one positive case, the NSLN metastasis was not significantly different from that in the OIn group （P=-0.118）. In the 1In group, clinical tumor size （P = 0.012）, over-expression of Her-2 （P=0.003）, tumor grade （P=0.018） and the total number of SLN （P=0.047） significantly correlated with non-SLN metastasis. Clinical tumor size （P=0.015） and the expression of Her-2 （P=0.01） were independent predictive factors for non-SLN metastasis by the Logistic regression model. Conclusion Under certain conditions, breast cancer patients with single SLN metastasis could avoid ALND.展开更多
Background Osteopontin （OPN） is a secreted phosphoglycoprotein （SSP） that is overexpressed in a variety of tumors and was regarded as a molecular marker of tumors.In this study,we intended to demonstrate the role ...Background Osteopontin （OPN） is a secreted phosphoglycoprotein （SSP） that is overexpressed in a variety of tumors and was regarded as a molecular marker of tumors.In this study,we intended to demonstrate the role of OPN in human breast cancer cell line MDA-MB-231.Methods Recombinant plasmid expressing small interfering RNA （siRNA） specific to OPN mRNA was transfected into MDA-MB-231 cells to generate the stable transfected cell line MDA-MB-343,and the empty plasmid tansfected cells （MDA-MB-neg） or wildtype MDA-MB-231 cells were used as control cells respectively.Expression of OPN,hypoxia inducible factor-1 （HIF-1） and vascular endothelial growth factor （VEGF） proteins was analyzed by Western blotting analysis.The radiosensitivity of cells was determined by detecting cell apoptosis,cell proliferation and cell senescence.Results HIF-1 and VEGF proteins in MDA-MB-343 cells were significantly downregulated upon the efficient knockdown of OPN expression under either hypoxia or normoxia environment.Moreover,expression of OPN protein was upregualted upon hypoxic culture.Stable OPN-silencing also decreased cell invasion,increased cell apoptosis and cell senescence,as well as reduced clonogenic survival,resulting in increase radiation tolerance.Conclusions Suppression of OPN gene expression can enhance radiosensitivity and affect cell apoptosis in breast cancer cells.OPN seems to be an attractive target for the improvement of radiotherapy.展开更多
基金funded by the Changchun Science and Technology Program (08KZ39)
文摘[ Objective] To observe the expression of telomerase in antler of sika deer during growth stage and thus to provide a new line for revealing the mechanisms of development and regeneration of antlers. [Method] The updated TRAP （Telomedc repeat amplification protocol） argentumdying method was used to detect the activity of telomerase in mesenchyme, precartilage, cartilage and ossification tissue of antler tip during the growth stage. Reverse transcription polymerase chain reaction （RT-PCR） was used to detect the expression of telomerase reverse transcriptase （TERT） in these cells. I-Result] The activity of telomerase was detected in the mesanchyme, precartilage and cartilage but not in the sclerotic tissue. The relative telomerase activity in the mesanchyme, precartilage and cartilage was 91.2, 46.4 and 13.7, respectively. In addition, the results of the expression of TERT gene also supported the detection result of telomerase activity. I-Conclmionl During growth phase, telomerase is expressed in multiplication area of deer antlers, and its activity decreases gradually with further differentiation of cells in antlers. Therefore, telomerase may play a key role in develoomant and rsoaneration of antlers.
文摘Background The purpose of this study was to investigate the feasibility of avoiding axillary lymph node dissection （ALND） for patients with only one sentinel lymph node （SLN） metastasis. The characteristics and predictive factors for non-sentinel lymph node （NSLN） metastasis of patients with single positive SLN were also analyzed. Methods Patients with no and only one SLN metastasis （OIn and 1In group, n ≥2） were selected from 1228 cases of invasive breast carcinoma, who underwent axillary dissection in Shandong Cancer Hospital between November 1999 and December 2011, to compare the characteristics of NSLN metastasis between them. For the 1In group, the factors that influenced the NSLN metastasis were analyzed by univariate and multivariate analysis. Results Differences of the NSLN metastasis between the OIn and the 1In groups were significant （P 〈0.001）. There was no significant difference between the axillary lymph node metastasis on level III in 1In group and OIn group （P=0.570）. When the total SLN number was 〉4 and with one positive case, the NSLN metastasis was not significantly different from that in the OIn group （P=-0.118）. In the 1In group, clinical tumor size （P = 0.012）, over-expression of Her-2 （P=0.003）, tumor grade （P=0.018） and the total number of SLN （P=0.047） significantly correlated with non-SLN metastasis. Clinical tumor size （P=0.015） and the expression of Her-2 （P=0.01） were independent predictive factors for non-SLN metastasis by the Logistic regression model. Conclusion Under certain conditions, breast cancer patients with single SLN metastasis could avoid ALND.
文摘Background Osteopontin （OPN） is a secreted phosphoglycoprotein （SSP） that is overexpressed in a variety of tumors and was regarded as a molecular marker of tumors.In this study,we intended to demonstrate the role of OPN in human breast cancer cell line MDA-MB-231.Methods Recombinant plasmid expressing small interfering RNA （siRNA） specific to OPN mRNA was transfected into MDA-MB-231 cells to generate the stable transfected cell line MDA-MB-343,and the empty plasmid tansfected cells （MDA-MB-neg） or wildtype MDA-MB-231 cells were used as control cells respectively.Expression of OPN,hypoxia inducible factor-1 （HIF-1） and vascular endothelial growth factor （VEGF） proteins was analyzed by Western blotting analysis.The radiosensitivity of cells was determined by detecting cell apoptosis,cell proliferation and cell senescence.Results HIF-1 and VEGF proteins in MDA-MB-343 cells were significantly downregulated upon the efficient knockdown of OPN expression under either hypoxia or normoxia environment.Moreover,expression of OPN protein was upregualted upon hypoxic culture.Stable OPN-silencing also decreased cell invasion,increased cell apoptosis and cell senescence,as well as reduced clonogenic survival,resulting in increase radiation tolerance.Conclusions Suppression of OPN gene expression can enhance radiosensitivity and affect cell apoptosis in breast cancer cells.OPN seems to be an attractive target for the improvement of radiotherapy.
