Objective:Alzheimer’s disease( AD) is the most common neurodegenerative disorder which is characterized by amyloid-β( Aβ) aggradation in the brain and impairment of cognitive function. Thioredoxin-1( Trx-1) is a re...Objective:Alzheimer’s disease( AD) is the most common neurodegenerative disorder which is characterized by amyloid-β( Aβ) aggradation in the brain and impairment of cognitive function. Thioredoxin-1( Trx-1) is a redox regulating protein,and plays roles in resisting the oxidative stress and protecting neurons. Our previous study found that Trx-1 improved the cognitive function of Parkinson’s Disease( PD) mice. Geranylgeranylacetone( GGA) is an antiulcer drug and induces the expression of Trx-1 in vivo and in vitro. However,whether Trx-1 improves cognitive functions in mice of APP/PS1 or GGA protects SH-SY5 Y cells from cytotoxicity induced by Aβ is still unknown. The objective of present is to investigate the roles of Trx-1 and GGA in inhibiting neurotoxicity of Aβ. Methods:We used MTT assay to test the cell viability induced by Aβ(25-35) and western blot to detect the expression of Trx-1 in SH-SY5 Y cells. Trx-1 overexpression transgenic mice were hybridized with APP/PS1 transgenic mice to get control,Trx-1,Tx-1/APP/PS1 and APP/PS1 mice. Then we used Morris water maze,high plus maze and object recognition test to detect the cognitive function of different kinds of mice. We also used RT-PCR and western blot to test the mRNA level and expression of Trx-1,APP,PS1 and Aβ. Results:In our present study,we demonstrated that Aβ(25-35) decreased the cell viability and the expression of Trx-1 in SH-SY5 Y cells. The cell viability and the expression of Trx-1 were reversed by GGA. Our results showed that the escape latency in APP/PS1 mice was longer when compared with the Trx-1/APP/PS1 mice in Morris water maze and high plus maze. Whereas navigational experiments in Morris water maze result showed that the total number of crossings and the percentage of time spent in the target quadrant were significantly decreased in APP/PS1 mice when compared to Trx-1/APP/PS1 mice. Object recognition test the discrimination index was significantly decreased in APP/PS1 mice when compared with Trx-1/APP/PS1 mice. The mRNA levels and the expression of APP,PS1 and Aβ were decreased in Trx-1/APP/PS1 mice when compared to APP/PS1 mice. Conclusion:These results suggest that GGA protects SH-SY5 Y cells from cytotoxicity induced by Aβ(25-35) and restored the expression of Trx-1. Trx-1 overexpression improves cognitive function of APP/PS1 mice. Trx-1 may be a potential therapeutic target for the clinical management of AD.展开更多
Background Low potassium dextran （LPD） solution can attenuate acute lung injury （ALl）. However, LPD solution for treating acute kidney injury secondary to ALl has not been reported. The present study was performed...Background Low potassium dextran （LPD） solution can attenuate acute lung injury （ALl）. However, LPD solution for treating acute kidney injury secondary to ALl has not been reported. The present study was performed to examine the renoprotective effect of LPD solution in ALl induced by oleic acid （OA） in piglets. Methods Twelve animals that suffered an ALl induced by administration of OA into the right atrium were divided into two groups： the placebo group （n=6） pretreated with normal saline and the LPD group （n=6）, pretreated with LPD solution. LPD solution was injected intravenously at a dose of 12.5 ml/kg via the auricular vein 1 hour before OA injection. Results All animals survived the experiments with mild histopathological injury to the kidney. There were no significant differences in mean arterial pressure （MAP）, creatinin and renal damage scores between the two groups. Compared with the placebo group, the LPD group had better gas exchange parameters at most of the observation points （（347.0±12.6） mmHg vs. （284.3±11.3） mmHg at 6 hours after ALl, P 〈0.01）. After 6 hours of treatment with OA, the plasma concentrations of NGAL and intedeukin （IL）-6 in both groups increased dramatically compared to baseline （（6.0±0.6） and （2.50±0.08） folds in placebo group; and （2.5±0.5） and （1.40±0.05） folds in LPD group）, but the change of both parameters in the LPD group was significantly lower （P 〈0.01） than in the placebo group. And 6 hours after ALl the kidney tissue concentration of IL-6 in the LPD group （（165.7 ± 22.5） pg·m-1·g-1 protein） was significantly lower （P 〈0.01） than that in placebo group （（67.2± 25.3） pg·m-1·g-1 protein）. Conclusion These findings suggest that pretreatment with LPD solution via systemic administration might attenuate acute kidney injury and the cytokine response of IL-6 in the ALl piglet model induced by OA injection.展开更多
利用简单的自组装法合成了二维PANI@MXene复合材料,其中MXene作为导电网络,提高了PANI的电导率,缩短了在PANI中的离子运输路径。这使得具有最佳质量比例的PANI_(0.5)@MXene展现出608 F g^(-1)的高比电容和良好的耐低温性能(-30℃时容量...利用简单的自组装法合成了二维PANI@MXene复合材料,其中MXene作为导电网络,提高了PANI的电导率,缩短了在PANI中的离子运输路径。这使得具有最佳质量比例的PANI_(0.5)@MXene展现出608 F g^(-1)的高比电容和良好的耐低温性能(-30℃时容量相对于室温时,保持率超过90%)。将PANI_(0.5)@MXene电极与过量的多孔MXene电极匹配,可构建具备1.3 V宽电压窗口的非对称赝电容超级电容器,以40 wt%硫酸为电解液,在20℃下容量可达32 mAh g^(-1)的容量,在-60℃下仍可保持室温下80%以上的容量。展开更多
文摘Objective:Alzheimer’s disease( AD) is the most common neurodegenerative disorder which is characterized by amyloid-β( Aβ) aggradation in the brain and impairment of cognitive function. Thioredoxin-1( Trx-1) is a redox regulating protein,and plays roles in resisting the oxidative stress and protecting neurons. Our previous study found that Trx-1 improved the cognitive function of Parkinson’s Disease( PD) mice. Geranylgeranylacetone( GGA) is an antiulcer drug and induces the expression of Trx-1 in vivo and in vitro. However,whether Trx-1 improves cognitive functions in mice of APP/PS1 or GGA protects SH-SY5 Y cells from cytotoxicity induced by Aβ is still unknown. The objective of present is to investigate the roles of Trx-1 and GGA in inhibiting neurotoxicity of Aβ. Methods:We used MTT assay to test the cell viability induced by Aβ(25-35) and western blot to detect the expression of Trx-1 in SH-SY5 Y cells. Trx-1 overexpression transgenic mice were hybridized with APP/PS1 transgenic mice to get control,Trx-1,Tx-1/APP/PS1 and APP/PS1 mice. Then we used Morris water maze,high plus maze and object recognition test to detect the cognitive function of different kinds of mice. We also used RT-PCR and western blot to test the mRNA level and expression of Trx-1,APP,PS1 and Aβ. Results:In our present study,we demonstrated that Aβ(25-35) decreased the cell viability and the expression of Trx-1 in SH-SY5 Y cells. The cell viability and the expression of Trx-1 were reversed by GGA. Our results showed that the escape latency in APP/PS1 mice was longer when compared with the Trx-1/APP/PS1 mice in Morris water maze and high plus maze. Whereas navigational experiments in Morris water maze result showed that the total number of crossings and the percentage of time spent in the target quadrant were significantly decreased in APP/PS1 mice when compared to Trx-1/APP/PS1 mice. Object recognition test the discrimination index was significantly decreased in APP/PS1 mice when compared with Trx-1/APP/PS1 mice. The mRNA levels and the expression of APP,PS1 and Aβ were decreased in Trx-1/APP/PS1 mice when compared to APP/PS1 mice. Conclusion:These results suggest that GGA protects SH-SY5 Y cells from cytotoxicity induced by Aβ(25-35) and restored the expression of Trx-1. Trx-1 overexpression improves cognitive function of APP/PS1 mice. Trx-1 may be a potential therapeutic target for the clinical management of AD.
基金This work was supported by grants from the National Natural Science Foundation of China （No. 30971380 and No. 31071026） and by the National Excellent Doctoral Dissertation of China.
文摘Background Low potassium dextran （LPD） solution can attenuate acute lung injury （ALl）. However, LPD solution for treating acute kidney injury secondary to ALl has not been reported. The present study was performed to examine the renoprotective effect of LPD solution in ALl induced by oleic acid （OA） in piglets. Methods Twelve animals that suffered an ALl induced by administration of OA into the right atrium were divided into two groups： the placebo group （n=6） pretreated with normal saline and the LPD group （n=6）, pretreated with LPD solution. LPD solution was injected intravenously at a dose of 12.5 ml/kg via the auricular vein 1 hour before OA injection. Results All animals survived the experiments with mild histopathological injury to the kidney. There were no significant differences in mean arterial pressure （MAP）, creatinin and renal damage scores between the two groups. Compared with the placebo group, the LPD group had better gas exchange parameters at most of the observation points （（347.0±12.6） mmHg vs. （284.3±11.3） mmHg at 6 hours after ALl, P 〈0.01）. After 6 hours of treatment with OA, the plasma concentrations of NGAL and intedeukin （IL）-6 in both groups increased dramatically compared to baseline （（6.0±0.6） and （2.50±0.08） folds in placebo group; and （2.5±0.5） and （1.40±0.05） folds in LPD group）, but the change of both parameters in the LPD group was significantly lower （P 〈0.01） than in the placebo group. And 6 hours after ALl the kidney tissue concentration of IL-6 in the LPD group （（165.7 ± 22.5） pg·m-1·g-1 protein） was significantly lower （P 〈0.01） than that in placebo group （（67.2± 25.3） pg·m-1·g-1 protein）. Conclusion These findings suggest that pretreatment with LPD solution via systemic administration might attenuate acute kidney injury and the cytokine response of IL-6 in the ALl piglet model induced by OA injection.
文摘利用简单的自组装法合成了二维PANI@MXene复合材料,其中MXene作为导电网络,提高了PANI的电导率,缩短了在PANI中的离子运输路径。这使得具有最佳质量比例的PANI_(0.5)@MXene展现出608 F g^(-1)的高比电容和良好的耐低温性能(-30℃时容量相对于室温时,保持率超过90%)。将PANI_(0.5)@MXene电极与过量的多孔MXene电极匹配,可构建具备1.3 V宽电压窗口的非对称赝电容超级电容器,以40 wt%硫酸为电解液,在20℃下容量可达32 mAh g^(-1)的容量,在-60℃下仍可保持室温下80%以上的容量。
