为了探析甜瓜(Cucumis melo L.)响应涝害胁迫的分子机制,采用转录组和蛋白组学方法研究了甜瓜耐涝型材料L45和涝敏感型材料L39在正常培养和涝害胁迫处理条件下的差异表达基因和蛋白。结果表明,L39-W vs L39-C的差异表达基因数为3532个,...为了探析甜瓜(Cucumis melo L.)响应涝害胁迫的分子机制,采用转录组和蛋白组学方法研究了甜瓜耐涝型材料L45和涝敏感型材料L39在正常培养和涝害胁迫处理条件下的差异表达基因和蛋白。结果表明,L39-W vs L39-C的差异表达基因数为3532个,差异表达蛋白数为105个;L45-W vs L45-C的差异表达基因数为1842个,差异表达蛋白数为38个。转录组与蛋白组联合分析发现,L39-W vs L39-C中38个基因在mRNA和蛋白质水平上联合上调表达,12个基因联合下调表达;L45-W vs L45-C中7个基因联合上调表达,3个基因联合下调表达。KEGG显著性富集分析结果发现,L39-W vs L39-C中联合上调表达的基因显著富集在类黄酮生物合成、苯丙烷生物合成、苯丙氨酸代谢、缬氨酸、亮氨酸和异亮氨酸生物合成、精氨酸和脯氨酸代谢等;而L45-W vs L45-C中联合上调表达的基因显著富集在α-亚麻酸代谢、类黄酮生物合成、苯丙烷生物合成、亚油酸代谢等。本研究联合转录组与蛋白组数据进行了差异表达基因与蛋白分析,为进一步挖掘甜瓜响应涝害胁迫的关键基因及通路奠定了一定基础。展开更多
Purpose-Temperature is an important load for a ballastless track.However,little research has been conducted on the dynamic responses when a train travels on a ballastless track under the temperature gradient.The dynam...Purpose-Temperature is an important load for a ballastless track.However,little research has been conducted on the dynamic responses when a train travels on a ballastless track under the temperature gradient.The dynamic responses under different temperature gradients of the slab are theoretically investigated in this work.Design/methodology/approach-Considering the moving train,the temperature gradient of the slab,and the gravity of the slab track,a dynamic model for a high-speed train that runs along the CRTS Ⅲ slab track on subgrade is developed by a nonlinear coupled way in Abaqus.Findings-The results are as follows:(1)The upward transmission of the periodic deformation of the slab causes periodic track irregularity.(2)Because of the geometric constraint of limiting structures,the maximum bending stresses of the slab occur near the end of the slab under positive temperature gradients,but in the middle of the slab under negative temperature gradients.(3)The periodic deformation of the slab can induce periodic changes in the interlayer stiffness and contact status,leading to a large vibration of the slab.Because of the vibration-reduction capacity of the fastener and the larger mass of the concrete base,the accelerations of both the slab and concrete base are far less than the acceleration of the rail.Originality/value-This study reveals the influence mechanism of temperature gradient-induced periodic deformation in the dynamic responses of the train-track system,and it also provides a guide for the safe service of CRTS Ⅲ slab track.展开更多
文摘为了探析甜瓜(Cucumis melo L.)响应涝害胁迫的分子机制,采用转录组和蛋白组学方法研究了甜瓜耐涝型材料L45和涝敏感型材料L39在正常培养和涝害胁迫处理条件下的差异表达基因和蛋白。结果表明,L39-W vs L39-C的差异表达基因数为3532个,差异表达蛋白数为105个;L45-W vs L45-C的差异表达基因数为1842个,差异表达蛋白数为38个。转录组与蛋白组联合分析发现,L39-W vs L39-C中38个基因在mRNA和蛋白质水平上联合上调表达,12个基因联合下调表达;L45-W vs L45-C中7个基因联合上调表达,3个基因联合下调表达。KEGG显著性富集分析结果发现,L39-W vs L39-C中联合上调表达的基因显著富集在类黄酮生物合成、苯丙烷生物合成、苯丙氨酸代谢、缬氨酸、亮氨酸和异亮氨酸生物合成、精氨酸和脯氨酸代谢等;而L45-W vs L45-C中联合上调表达的基因显著富集在α-亚麻酸代谢、类黄酮生物合成、苯丙烷生物合成、亚油酸代谢等。本研究联合转录组与蛋白组数据进行了差异表达基因与蛋白分析,为进一步挖掘甜瓜响应涝害胁迫的关键基因及通路奠定了一定基础。
基金supported by National Key R&D Program of China[Grant No.2022YFB2603400]R&D Project of China State Railway Group Corporation Limited[Grant No.P2021G053]R&D Project of China Academy of Railway Science Corporation Limited[Grant No.2023YJ200].
文摘Purpose-Temperature is an important load for a ballastless track.However,little research has been conducted on the dynamic responses when a train travels on a ballastless track under the temperature gradient.The dynamic responses under different temperature gradients of the slab are theoretically investigated in this work.Design/methodology/approach-Considering the moving train,the temperature gradient of the slab,and the gravity of the slab track,a dynamic model for a high-speed train that runs along the CRTS Ⅲ slab track on subgrade is developed by a nonlinear coupled way in Abaqus.Findings-The results are as follows:(1)The upward transmission of the periodic deformation of the slab causes periodic track irregularity.(2)Because of the geometric constraint of limiting structures,the maximum bending stresses of the slab occur near the end of the slab under positive temperature gradients,but in the middle of the slab under negative temperature gradients.(3)The periodic deformation of the slab can induce periodic changes in the interlayer stiffness and contact status,leading to a large vibration of the slab.Because of the vibration-reduction capacity of the fastener and the larger mass of the concrete base,the accelerations of both the slab and concrete base are far less than the acceleration of the rail.Originality/value-This study reveals the influence mechanism of temperature gradient-induced periodic deformation in the dynamic responses of the train-track system,and it also provides a guide for the safe service of CRTS Ⅲ slab track.
